2024 |
Pastirčáková, K; Baková, K; Adamčíková, K; Barta, M; Lalík, M; Pavlík, M; Kunca, A; Galko, J; Pastirčák, M Fungi associated with ambrosia beetle Xylosandrus germanus in Slovakia Journal Article Biologia, 79 (8), pp. 2387-2400, 2024, ISSN: 0006-3088. @article{Pastirčáková2024, title = {Fungi associated with ambrosia beetle \textit{Xylosandrus germanus} in Slovakia}, author = {K. Pastirčáková and K. Baková and K. Adamčíková and M. Barta and M. Lalík and M. Pavlík and A. Kunca and J. Galko and M. Pastirčák}, doi = {10.1007/s11756-024-01712-7}, issn = {0006-3088}, year = {2024}, date = {2024-08-21}, journal = {Biologia}, volume = {79}, number = {8}, pages = {2387-2400}, abstract = {The black timber bark beetle (Xylosandrus germanus) is a strongly invasive ambrosia beetle and an important forest pest in Slovakia. This pest is closely associated with symbiotic fungi used as its food source. We investigated the fungi associated with X. germanus adults in Slovakia. In this study, Beauveria bassiana, B. pseudobassiana, Clonostachys rosea, Fusarium oxysporum, Ophiostoma quercus, Phaeoacremonium scolyti, and Talaromyces amestolkiae were isolated and identified by morphological and molecular analyses. The fungus Ophiostoma quercus was most frequently isolated from living beetles, while the entomopathogenic Beauveria bassiana was the most commonly isolated from dead beetles. The morphological descriptions of fungi based on isolates from the surface of X. germanus adults are provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The black timber bark beetle (Xylosandrus germanus) is a strongly invasive ambrosia beetle and an important forest pest in Slovakia. This pest is closely associated with symbiotic fungi used as its food source. We investigated the fungi associated with X. germanus adults in Slovakia. In this study, Beauveria bassiana, B. pseudobassiana, Clonostachys rosea, Fusarium oxysporum, Ophiostoma quercus, Phaeoacremonium scolyti, and Talaromyces amestolkiae were isolated and identified by morphological and molecular analyses. The fungus Ophiostoma quercus was most frequently isolated from living beetles, while the entomopathogenic Beauveria bassiana was the most commonly isolated from dead beetles. The morphological descriptions of fungi based on isolates from the surface of X. germanus adults are provided. |
Jančovičová, S; Adamčíková, K; Caboň, M; Graddy, M G; Matheny, P B; Noffsinger, Ch. R; Wheeler, T B; Adamčík, S Taxonomic reintroduction of the holarctic saprotrophic fungus Crepidotus cinnamomeus Journal Article Mycological Progress, 23 , pp. 49, 2024, ISSN: 1617-416X. @article{Jančovičová2024, title = {Taxonomic reintroduction of the holarctic saprotrophic fungus Crepidotus cinnamomeus}, author = {S. Jančovičová and K. Adamčíková and M. Caboň and M. G. Graddy and P. B. Matheny and Ch. R. Noffsinger and T. B. Wheeler and S. Adamčík}, doi = {https://doi.org/10.1007/s11557-024-01991-z}, issn = {1617-416X}, year = {2024}, date = {2024-08-16}, journal = {Mycological Progress}, volume = {23}, pages = {49}, abstract = {Crepidotus is a genus of common saprotrophic fungi well known especially in the Northern Hemisphere, but distribution patterns of individual species are not sufficiently understood. We redefined a taxonomic circumscription of Crepidotus cinnamomeus based on morphological and molecular congruencies between the type material and recent collections. The species is well delimited from other similar and currently accepted species of the genus. Crepidotus cinnamomeus was found to have a broad holarctic distribution with occurrences in North America, Europe and Asia where it grows on twigs and branches of deciduous trees and shrubs in preferably cold humid habitats. Here we present the first multilocus phylogeny of the genus, including portions of the RPB2 gene. Our study highlights the importance of sufficient sampling from broader areas supported by sequence data, which is essential for estimation of species delimitation, distribution and correct name assignment for Crepidotus species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Crepidotus is a genus of common saprotrophic fungi well known especially in the Northern Hemisphere, but distribution patterns of individual species are not sufficiently understood. We redefined a taxonomic circumscription of Crepidotus cinnamomeus based on morphological and molecular congruencies between the type material and recent collections. The species is well delimited from other similar and currently accepted species of the genus. Crepidotus cinnamomeus was found to have a broad holarctic distribution with occurrences in North America, Europe and Asia where it grows on twigs and branches of deciduous trees and shrubs in preferably cold humid habitats. Here we present the first multilocus phylogeny of the genus, including portions of the RPB2 gene. Our study highlights the importance of sufficient sampling from broader areas supported by sequence data, which is essential for estimation of species delimitation, distribution and correct name assignment for Crepidotus species. |
Bradshaw, M; Braun, U; Götz, M; Dogan, G; Erdogdu, M; Ates, M A; Pastirčáková, K; Pastirčák, M; Fernández-Pavía, S; Takamatsu, S; Abbasi, M; Moparthi, S; Gafforov, Y; Chinan, V C; Kummer, V; Kruse, J; Pfister, D H Contributions to the knowledge of the phylogeny and taxonomy of the Erysiphaceae (powdery mildews) – part 2 Journal Article Sydowia, 76 , pp. 113-145, 2024, ISSN: 0082-0598. @article{Bradshaw2024, title = {Contributions to the knowledge of the phylogeny and taxonomy of the Erysiphaceae (powdery mildews) – part 2}, author = {M. Bradshaw and U. Braun and M. Götz and G. Dogan and M. Erdogdu and M.A. Ates and K. Pastirčáková and M. Pastirčák and S. Fernández-Pavía and S. Takamatsu and M. Abbasi and S. Moparthi and Y. Gafforov and V.C. Chinan and V. Kummer and J. Kruse and D.H. Pfister}, doi = {10.12905/0380.sydowia76-2024-0113}, issn = {0082-0598}, year = {2024}, date = {2024-05-06}, journal = {Sydowia}, volume = {76}, pages = {113-145}, abstract = {The phylogeny and taxonomy of several powdery mildews (Erysiphaceae) have been examined. Sequences of the rDNA ITS region have been retrieved from types and additional specimens, collected in Germany, Iran, Turkey, Uzbekistan, and several other countries. Erysiphe cruciferarum s. lat. has been revised and split into E. cruciferarum s. str. on Alyssum and Berteroa (tribe Alysseae), E. alliariicola sp. nov. on Alliaria petiolata, and E. radulescui, a plurivorous species that occurs on a wide range of cruciferous hosts as well as on Cleome spp. and Papaver spp. The revision of this complex is supported by phylogenetic multilocus analyses of rDNA (ITS, 28S and IGS) and protein coding genes (CAM, GAPDH, GS, RPB2, and TUB). The Erysiphe euphorbiicola complex is phylogenetically examined. Oidium poinsettiae is very closely allied to Erysiphe euphorbiicola, but final conclusions on the taxonomy of this species and its relation to E. euphorbiicola are not yet possible and require additional sequences and multilocus examinations. E. euphorbiae-cotinifoliae sp. nov. is described on Euphorbia cotinifolia from Mexico. Podosphaera aucupariae is epitypified, and the new variety P. aucupariae var. arcuatispora on Sorbus aucuparia in Turkey is described. Furthermore, the phylogeny and taxonomy of Erysiphe pachypodii, Euoidium parthenii-integrifolii, Golovinomyces clematidis, G. hyoscyami, G. prenanthis, G. senecionis on Ligularia persica and Senecio spp., Fibroidium pelargonii, and Podosphaera clandestina var. cydoniae have been examined. Podosphaera pannosa has been reported on Mandevilla splendens (Apocynaceae). – 2 new species, 5 epitypifications, 1 lectotypification, 4 new synonyms.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The phylogeny and taxonomy of several powdery mildews (Erysiphaceae) have been examined. Sequences of the rDNA ITS region have been retrieved from types and additional specimens, collected in Germany, Iran, Turkey, Uzbekistan, and several other countries. Erysiphe cruciferarum s. lat. has been revised and split into E. cruciferarum s. str. on Alyssum and Berteroa (tribe Alysseae), E. alliariicola sp. nov. on Alliaria petiolata, and E. radulescui, a plurivorous species that occurs on a wide range of cruciferous hosts as well as on Cleome spp. and Papaver spp. The revision of this complex is supported by phylogenetic multilocus analyses of rDNA (ITS, 28S and IGS) and protein coding genes (CAM, GAPDH, GS, RPB2, and TUB). The Erysiphe euphorbiicola complex is phylogenetically examined. Oidium poinsettiae is very closely allied to Erysiphe euphorbiicola, but final conclusions on the taxonomy of this species and its relation to E. euphorbiicola are not yet possible and require additional sequences and multilocus examinations. E. euphorbiae-cotinifoliae sp. nov. is described on Euphorbia cotinifolia from Mexico. Podosphaera aucupariae is epitypified, and the new variety P. aucupariae var. arcuatispora on Sorbus aucuparia in Turkey is described. Furthermore, the phylogeny and taxonomy of Erysiphe pachypodii, Euoidium parthenii-integrifolii, Golovinomyces clematidis, G. hyoscyami, G. prenanthis, G. senecionis on Ligularia persica and Senecio spp., Fibroidium pelargonii, and Podosphaera clandestina var. cydoniae have been examined. Podosphaera pannosa has been reported on Mandevilla splendens (Apocynaceae). – 2 new species, 5 epitypifications, 1 lectotypification, 4 new synonyms. |
Jánošíková, Z; Kobza, M; Ondrušková, E; Ostrovský, R; Pažitný, J; Adamčíková, K Virulence of Dothistroma septosporum and D. pini on Pinus nigra and P. mugo under conditions of natural infection Journal Article European Journal of Plant Pathology, 168 (2), pp. 775-785, 2024. @article{Jánošíková2024, title = {Virulence of Dothistroma septosporum and D. pini on Pinus nigra and P. mugo under conditions of natural infection}, author = {Z. Jánošíková and M. Kobza and E. Ondrušková and R. Ostrovský and J. Pažitný and K. Adamčíková}, url = {http://ife.sk/wp-content/uploads/2020/09/Fig_edited.jpghttp://ife.sk/wp-content/uploads/2020/09/final-1-2.jpg}, doi = {https://doi.org/10.1007/s10658-023-02799-5}, year = {2024}, date = {2024-04-04}, journal = {European Journal of Plant Pathology}, volume = {168}, number = {2}, pages = {775-785}, abstract = {Dothistroma needle blight (DNB) is a severe needle disease of pines worldwide, caused by two closely related species, Dothistroma septosporum and D. pini. The two fungal species are similar not only in their morphological characteristics, but also cause very similar symptoms in their hosts, and have a similar ecology. The aim of this study was to compare the virulence of the two Dothistroma species in natural infection experiments on 2-year-old seedlings of two DNB susceptible pine species, Pinus nigra and P. mugo, in two seedling stands for each pathogen species. The virulence of the pathogens and presence of symptoms (symptomatic needles, red bands and acervuli) were assessed after 2 years of exposure to inoculum. The incidence of seedlings with DNB symptoms was 65% and 76% for P. nigra and P. mugo, respectively. No difference was found between D. septosporum and D. pini in any of the three DNB symptoms evaluated on seedlings of P. mugo. However, symptoms of disease differed between the two Dothistroma species on P. nigra. Variables, which reflect the intensity of disease development, the number of red bands and acervuli per needle, showed a difference in virulence between D. septosporum and D. pini, but only in the case of the host species P. nigra. The results suggest that the virulence of the two Dothistroma species could be affected by host pine species and that there are differences in susceptibility of individual pine species to D. septosporum and D. pini. Further factors could affect the virulence of these pathogens, including isolate origin, climatic or environmental factors.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a severe needle disease of pines worldwide, caused by two closely related species, Dothistroma septosporum and D. pini. The two fungal species are similar not only in their morphological characteristics, but also cause very similar symptoms in their hosts, and have a similar ecology. The aim of this study was to compare the virulence of the two Dothistroma species in natural infection experiments on 2-year-old seedlings of two DNB susceptible pine species, Pinus nigra and P. mugo, in two seedling stands for each pathogen species. The virulence of the pathogens and presence of symptoms (symptomatic needles, red bands and acervuli) were assessed after 2 years of exposure to inoculum. The incidence of seedlings with DNB symptoms was 65% and 76% for P. nigra and P. mugo, respectively. No difference was found between D. septosporum and D. pini in any of the three DNB symptoms evaluated on seedlings of P. mugo. However, symptoms of disease differed between the two Dothistroma species on P. nigra. Variables, which reflect the intensity of disease development, the number of red bands and acervuli per needle, showed a difference in virulence between D. septosporum and D. pini, but only in the case of the host species P. nigra. The results suggest that the virulence of the two Dothistroma species could be affected by host pine species and that there are differences in susceptibility of individual pine species to D. septosporum and D. pini. Further factors could affect the virulence of these pathogens, including isolate origin, climatic or environmental factors. |
Noffsinger, Ch. R; Adamčíková, K; Eberhardt, U; Caboň, M; Bazzicalup, A; Buyck, B; Kaufmann, H; Weholt, Ø; Looney, B P; Matheny, P B; Berbee, M L; Tausan, D; Adamčík, S Three new species in Russula subsection Xerampelinae supported by genealogical and phenotypic coherence Journal Article Mycologia, 116 (2), pp. 322-349, 2024. @article{Noffsinger2024, title = {Three new species in Russula subsection Xerampelinae supported by genealogical and phenotypic coherence}, author = {Ch. R. Noffsinger and K. Adamčíková and U. Eberhardt and M. Caboň and A. Bazzicalup and B. Buyck and H. Kaufmann and Ø. Weholt and B. P. Looney and P. B. Matheny and M. L. Berbee and D. Tausan and S. Adamčík}, doi = {10.1080/00275514.2023.2295957}, year = {2024}, date = {2024-02-16}, journal = {Mycologia}, volume = {116}, number = {2}, pages = {322-349}, abstract = {Xerampelinae is a subsection composed of species of ectomycorrhizal fungi belonging to the hyperdiverse and cosmopolitan genus Russula (Russulales). Species of Xerampelinae are recognized by their fishy or shrimp odor, browning context, and a green reaction to iron sulfate. However, species delimitation has traditionally relied on morphology and analysis of limited molecular data. Prior taxonomic work in Xerampelinae has led to the description of as many as 59 taxa in Europe and 19 in North America. Here we provide the first multilocus phylogeny of European and North American members based on two nrDNA loci and two protein-coding genes. The resulting phylogeny supports the recognition of 17 species-rank Xerampelinae clades; however, higher species richness (~23) is suggested by a more inclusive nuclear rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS barcode) analysis. Phylogenetic and morphological analyses support three new species with restricted geographic distributions: R. lapponica, R. neopascua, and R. olympiana. We confirm that the European species R. subrubens is present in North America and the North American species R. serissima previously known as R. favrei) is present in Europe. Most other Xerampelinae appear restricted to either North America or Eurasia, which indicates a high degree of regional endemism; this includes R. xerampelina, a name widely applied to North American taxa, but a species restricted to Eurasia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Xerampelinae is a subsection composed of species of ectomycorrhizal fungi belonging to the hyperdiverse and cosmopolitan genus Russula (Russulales). Species of Xerampelinae are recognized by their fishy or shrimp odor, browning context, and a green reaction to iron sulfate. However, species delimitation has traditionally relied on morphology and analysis of limited molecular data. Prior taxonomic work in Xerampelinae has led to the description of as many as 59 taxa in Europe and 19 in North America. Here we provide the first multilocus phylogeny of European and North American members based on two nrDNA loci and two protein-coding genes. The resulting phylogeny supports the recognition of 17 species-rank Xerampelinae clades; however, higher species richness (~23) is suggested by a more inclusive nuclear rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS barcode) analysis. Phylogenetic and morphological analyses support three new species with restricted geographic distributions: R. lapponica, R. neopascua, and R. olympiana. We confirm that the European species R. subrubens is present in North America and the North American species R. serissima previously known as R. favrei) is present in Europe. Most other Xerampelinae appear restricted to either North America or Eurasia, which indicates a high degree of regional endemism; this includes R. xerampelina, a name widely applied to North American taxa, but a species restricted to Eurasia. |
2023 |
Brindza, Ján; Ďurišová, Ľuba; Ostrovský, Radovan Morfologická charakteristika peľových zŕn niektorých medonosných druhov rastlín Book AgroBioNet, Slovenská poľnohospodárska univerzita v Nitre, 2023, ISBN: 978–80–552‐2708‐5. @book{Brindza31.1, title = {Morfologická charakteristika peľových zŕn niektorých medonosných druhov rastlín}, author = {Ján Brindza and Ľuba Ďurišová and Radovan Ostrovský}, url = {http://www.slpk.sk/eldo/2023/dl/9788055227085/9788055227085.html}, doi = {https://doi.org/10.15414/2023.9788055227085}, isbn = {978–80–552‐2708‐5}, year = {2023}, date = {2023-12-31}, booktitle = {Morfologická charakteristika peľových zŕn niektorých medonosných druhov rastlín}, pages = {119}, publisher = {Slovenská poľnohospodárska univerzita v Nitre}, edition = {AgroBioNet}, abstract = {The aim of the monography is presentation of pollen grain characteristics from selected group of plant species. The evaluated group consists of plants that are traditionally cultivated such as Helianthus annuus L., Prunus avium (L.) L.; rarely used such as Castanea sativa Mill., Aronia melanocarpa (Michx.) Elliott; less known species Diospyros kaki L.; invasive species Solidago gigantea Aiton; ornamental species Mahonia aquifolium (Pursh) Nutt., naturally occurring plant species Taraxacum sect. Ruderalia, Lamium purpureum L., Cichorium intybus L, Crataegus monogyna Jacq.; as well as species used for bees’ pasture in Slovakia such as Phacelia tanacetifolia Benth., from which bees are gathering nectar and pollen for their feeding. Flowers were gathered from selected plants at the start of full bloom. In laboratory conditions, the pollen was released from stamen and subsequently dried and placed on stubs to be prepared for observation and photographed on scanning electron microscope. Pollen grains were classified based on internationally recognized methodology (Ertdtman, 1952). We evaluated fundamental morphological characteristics of pollen grains – size of grains based on length of polar (P) and equatorial (E) axis, shape of pollen grains (P/E), outline, pollen unit, polarity of pollen grains, symmetry, number and shape of apertures, exine sculpture. Results confirmed that identifying characteristics, also applicable for detection of species spectrum of pollen in honey, is unique and steady exine sculpture as well as number and shape of apertures, which is documented by SEM observations especially on details of surface sculpture. We determined length of polar axis (P) in range from 20.51 μm (Castanea sativa Mill.) to 58.03 μm (Diospyros kaki L.), length of equatorial axis (E) in range from 9.37 μm (Castanea sativa Mill.) to 39.05 μm (Cichorium intybus L.) and shape index (P/E) in range from 1.00 (Cichorium intybus L.) to 2.34 (Phacelia tanacetifolia Benth.). Acquired results will be used for creation of database of pollen grains from plant species growing in Slovakia and also for evaluation of botanical and geographical origin of honey samples and other bees’ products, which will serve the apiculture public and other target groups in Slovakia. Group of evaluated plants in this monography represents the preview of oncoming extended set of plant species in frame of international palynological database of Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {book} } The aim of the monography is presentation of pollen grain characteristics from selected group of plant species. The evaluated group consists of plants that are traditionally cultivated such as Helianthus annuus L., Prunus avium (L.) L.; rarely used such as Castanea sativa Mill., Aronia melanocarpa (Michx.) Elliott; less known species Diospyros kaki L.; invasive species Solidago gigantea Aiton; ornamental species Mahonia aquifolium (Pursh) Nutt., naturally occurring plant species Taraxacum sect. Ruderalia, Lamium purpureum L., Cichorium intybus L, Crataegus monogyna Jacq.; as well as species used for bees’ pasture in Slovakia such as Phacelia tanacetifolia Benth., from which bees are gathering nectar and pollen for their feeding. Flowers were gathered from selected plants at the start of full bloom. In laboratory conditions, the pollen was released from stamen and subsequently dried and placed on stubs to be prepared for observation and photographed on scanning electron microscope. Pollen grains were classified based on internationally recognized methodology (Ertdtman, 1952). We evaluated fundamental morphological characteristics of pollen grains – size of grains based on length of polar (P) and equatorial (E) axis, shape of pollen grains (P/E), outline, pollen unit, polarity of pollen grains, symmetry, number and shape of apertures, exine sculpture. Results confirmed that identifying characteristics, also applicable for detection of species spectrum of pollen in honey, is unique and steady exine sculpture as well as number and shape of apertures, which is documented by SEM observations especially on details of surface sculpture. We determined length of polar axis (P) in range from 20.51 μm (Castanea sativa Mill.) to 58.03 μm (Diospyros kaki L.), length of equatorial axis (E) in range from 9.37 μm (Castanea sativa Mill.) to 39.05 μm (Cichorium intybus L.) and shape index (P/E) in range from 1.00 (Cichorium intybus L.) to 2.34 (Phacelia tanacetifolia Benth.). Acquired results will be used for creation of database of pollen grains from plant species growing in Slovakia and also for evaluation of botanical and geographical origin of honey samples and other bees’ products, which will serve the apiculture public and other target groups in Slovakia. Group of evaluated plants in this monography represents the preview of oncoming extended set of plant species in frame of international palynological database of Slovakia. |
Takov, Danail; Barta, Marek; Nikolova, Milena; Doychev, Danail; Toshova, Teodora; Ostoich, Peter; Pilarska, Daniela Insecticidal activity of three plant extracts against adult Ips typograpgus L. under laboratory conditions Journal Article Baltic Journal of Coleopterology, 23 (2), pp. 139-158, 2023, ISSN: 1407 – 8619. @article{Takov2023, title = {Insecticidal activity of three plant extracts against adult \textit{Ips typograpgus} L. under laboratory conditions}, author = {Danail Takov and Marek Barta and Milena Nikolova and Danail Doychev and Teodora Toshova and Peter Ostoich and Daniela Pilarska}, url = {https://doi.org/10.59893/bjc.23(2).001}, doi = {10.59893/bjc.23(2).001}, issn = {1407 – 8619}, year = {2023}, date = {2023-12-08}, journal = {Baltic Journal of Coleopterology}, volume = {23}, number = {2}, pages = {139-158}, abstract = {Three plant extracts - Origanum vulgare subsp. hirtum essential oil (EO), Monarda fistulosa EO, and a hexane fraction of Tanacetum cinerariifolium, wеre evaluated and compared regarding their insecticide activity under laboratory conditions against the adults of the European spruce bark beetle, Ips typographus. The viability of I. typographus was affected with all tested plant extracts. The insecticidal effect varied among the extracts and concentrations used. Generally the mortality of beetles increased with the concentration of extracts and a significant positive correlation between the cumulative mortality and the extract concentration was confirmed by Pearson’s correlation analysis (r = 0.910, p = 0.012 for O. vulgare subsp. hirtum, r = 0.937, p = 0.005 for T. cinerariifolium, r = 0.814, p = 0.048 for M. fistulosa). At the highest extract concentration (10%), the total cumulative mortalities reached 98% for O. vulgare subsp. hirtum and T. cinerariifolium extracts at the end of the bioassay (four days after treatment). The exposure of beetles to M. fistulosa extract decreased the survivability of I. typographus the most (15%) and was followed by T. cinerariifolium (38%) and O. vulgare subsp. hirtum (43%). Log-rank test showed no significant difference in the survival probabilities among the extracts. These are the first tests on the insecticidal effect of plant extracts from Tanacetum cineranifolium and Monarda fistulosa against the European spruce bark beetle Ips typographus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three plant extracts - Origanum vulgare subsp. hirtum essential oil (EO), Monarda fistulosa EO, and a hexane fraction of Tanacetum cinerariifolium, wеre evaluated and compared regarding their insecticide activity under laboratory conditions against the adults of the European spruce bark beetle, Ips typographus. The viability of I. typographus was affected with all tested plant extracts. The insecticidal effect varied among the extracts and concentrations used. Generally the mortality of beetles increased with the concentration of extracts and a significant positive correlation between the cumulative mortality and the extract concentration was confirmed by Pearson’s correlation analysis (r = 0.910, p = 0.012 for O. vulgare subsp. hirtum, r = 0.937, p = 0.005 for T. cinerariifolium, r = 0.814, p = 0.048 for M. fistulosa). At the highest extract concentration (10%), the total cumulative mortalities reached 98% for O. vulgare subsp. hirtum and T. cinerariifolium extracts at the end of the bioassay (four days after treatment). The exposure of beetles to M. fistulosa extract decreased the survivability of I. typographus the most (15%) and was followed by T. cinerariifolium (38%) and O. vulgare subsp. hirtum (43%). Log-rank test showed no significant difference in the survival probabilities among the extracts. These are the first tests on the insecticidal effect of plant extracts from Tanacetum cineranifolium and Monarda fistulosa against the European spruce bark beetle Ips typographus. |
Jánošíková, Z; Ondrušková, E; Adamčíková, K Artificial inoculation and susceptibility of Pinus armandii to Dothistroma septosporum Journal Article Forest Pathology, 53 (5), pp. e12826, 2023, ISSN: 1439-0329. @article{Jánošíková2023b, title = {Artificial inoculation and susceptibility of Pinus armandii to Dothistroma septosporum}, author = {Z. Jánošíková and E. Ondrušková and K. Adamčíková}, doi = {DOI: 10.1111/efp.12826 }, issn = {1439-0329}, year = {2023}, date = {2023-08-18}, journal = {Forest Pathology}, volume = {53}, number = {5}, pages = {e12826}, abstract = {Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species. |
Adamčíková, K; Pastirčáková, K; Jánošíková, Z; Ostrovský, R; Pastirčák, M; Pažitný, J; Kobza, M; Adamčík, S; Kádasi-Horáková, M; Ondrušková, E New regional records of Dothistroma needle blight pathogens from Slovakia: distribution, hosts and pathogens characterization Journal Article Annals of Forest Research, 66 (1), pp. 99-111, 2023, ISSN: 1844-8135. @article{Adamčíková2023, title = {New regional records of Dothistroma needle blight pathogens from Slovakia: distribution, hosts and pathogens characterization}, author = {K. Adamčíková and K. Pastirčáková and Z. Jánošíková and R. Ostrovský and M. Pastirčák and J. Pažitný and M. Kobza and S. Adamčík and M. Kádasi-Horáková and E. Ondrušková}, doi = {https://doi.org/10.15287/afr.2023.2427}, issn = {1844-8135}, year = {2023}, date = {2023-07-25}, journal = {Annals of Forest Research}, volume = {66}, number = {1}, pages = {99-111}, abstract = {Dothistroma needle blight is one of the most severe needle diseases of pines caused by two closely related species, Dothistroma septosporum and D. pini. The further spread and distribution of this disease was investigated in Slovakia as well as new hosts and stand types were identified at regional level. Dothistroma septosporum was recorded in a natural stand at higher altitude on Pinus cembra in the High Tatras and the P. uncinata records are new host reports for Slovakia for this pathogen. Moreover, for D. pini, P. cembra as a new host at the country level was recorded and P. armandii was identified as new host species worldwide. Mating types for all collected samples and ITS haplotypes for D. pini isolates were determined. For D. pini, five localities with the presence of both mating types and three ITS haplotypes (Dp_HAP.1, Dp_HAP.2 and Dp_HAP.4) were reported. Samples where both mating types of the pathogens were identified, were selected for the microscopic examination of fruiting bodies aimed to detect sexual reproductive organs. In all inspected needle samples of D. pini, only conidiomata with typical hyaline cylindrical conidia were identified. The sexual state of D. septosporum was recorded in one sample of P. nigra needles.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight is one of the most severe needle diseases of pines caused by two closely related species, Dothistroma septosporum and D. pini. The further spread and distribution of this disease was investigated in Slovakia as well as new hosts and stand types were identified at regional level. Dothistroma septosporum was recorded in a natural stand at higher altitude on Pinus cembra in the High Tatras and the P. uncinata records are new host reports for Slovakia for this pathogen. Moreover, for D. pini, P. cembra as a new host at the country level was recorded and P. armandii was identified as new host species worldwide. Mating types for all collected samples and ITS haplotypes for D. pini isolates were determined. For D. pini, five localities with the presence of both mating types and three ITS haplotypes (Dp_HAP.1, Dp_HAP.2 and Dp_HAP.4) were reported. Samples where both mating types of the pathogens were identified, were selected for the microscopic examination of fruiting bodies aimed to detect sexual reproductive organs. In all inspected needle samples of D. pini, only conidiomata with typical hyaline cylindrical conidia were identified. The sexual state of D. septosporum was recorded in one sample of P. nigra needles. |
Franić, Iva; Allan, Eric; Prospero, Simone; Adamson, Kalev; Attorre, Fabio; Auger-Rozenberg, Marie-Anne; Augustin, Sylvie; Avtzis, Dimitrios; Baert, Wim; Barta, Marek; Bauters, Kenneth; Bellahirech, Amani; Boroń, Piotr; Bragança, Helena; Brestovanská, Tereza; Brurberg, May Bente; Burgess, Treena; Burokienė, Daiva; Cleary, Michelle; Corley, Juan; Coyle, David R; Csóka, György; Černý, Karel; Davydenko, Kateryna; de Groot, Maarten; Diez, Julio Javier; Lehtijärvi, Tugba Doğmuş H; Drenkhan, Rein; Edwards, Jacqueline; Elsafy, Mohammed; Eötvös, Csaba Béla; Falko, Roman; Fan, Jianting; Feddern, Nina; Fürjes-Mikó, Ágnes; Gossner, Martin M; Grad, Bartłomiej; Hartmann, Martin; Havrdova, Ludmila; Horáková, Miriam Kádasi; Hrabětová, Markéta; Justesen, Mathias Just; Kacprzyk, Magdalena; Kenis, Marc; Kirichenko, Natalia; Kovač, Marta; Kramarets, Volodymyr; Lacković, Nikola; Lantschner, Maria Victoria; Lazarević, Jelena; Leskiv, Marianna; Li, Hongmei; Madsen, Corrie Lynne; Malumphy, Chris; Matošević, Dinka; Matsiakh, Iryna; May, Tom W; Meffert, Johan; Migliorini, Duccio; Nikolov, Christo; O’Hanlon, Richard; Oskay, Funda; Paap, Trudy; Parpan, Taras; Piškur, Barbara; Ravn, Hans Peter; Richard, John; Ronse, Anne; Roques, Alain; Ruffner, Beat; Santini, Alberto; Sivickis, Karolis; Soliani, Carolina; Talgø, Venche; Tomoshevich, Maria; Uimari, Anne; Ulyshen, Michael; Vettraino, Anna Maria; Villari, Caterina; Wang, Yongjun; Witzell, Johanna; Zlatković, Milica; Eschen, René Climate, host and geography shape insect and fungal communities of trees Journal Article Scientific Reports, 13 , pp. 11570 , 2023, ISSN: 2045-2322. @article{Franić2023, title = {Climate, host and geography shape insect and fungal communities of trees}, author = {Iva Franić and Eric Allan and Simone Prospero and Kalev Adamson and Fabio Attorre and Marie-Anne Auger-Rozenberg and Sylvie Augustin and Dimitrios Avtzis and Wim Baert and Marek Barta and Kenneth Bauters and Amani Bellahirech and Piotr Boroń and Helena Bragança and Tereza Brestovanská and May Bente Brurberg and Treena Burgess and Daiva Burokienė and Michelle Cleary and Juan Corley and David R. Coyle and György Csóka and Karel Černý and Kateryna Davydenko and Maarten de Groot and Julio Javier Diez and H. Tugba Doğmuş Lehtijärvi and Rein Drenkhan and Jacqueline Edwards and Mohammed Elsafy and Csaba Béla Eötvös and Roman Falko and Jianting Fan and Nina Feddern and Ágnes Fürjes-Mikó and Martin M. Gossner and Bartłomiej Grad and Martin Hartmann and Ludmila Havrdova and Miriam Kádasi Horáková and Markéta Hrabětová and Mathias Just Justesen and Magdalena Kacprzyk and Marc Kenis and Natalia Kirichenko and Marta Kovač and Volodymyr Kramarets and Nikola Lacković and Maria Victoria Lantschner and Jelena Lazarević and Marianna Leskiv and Hongmei Li and Corrie Lynne Madsen and Chris Malumphy and Dinka Matošević and Iryna Matsiakh and Tom W. May and Johan Meffert and Duccio Migliorini and Christo Nikolov and Richard O’Hanlon and Funda Oskay and Trudy Paap and Taras Parpan and Barbara Piškur and Hans Peter Ravn and John Richard and Anne Ronse and Alain Roques and Beat Ruffner and Alberto Santini and Karolis Sivickis and Carolina Soliani and Venche Talgø and Maria Tomoshevich and Anne Uimari and Michael Ulyshen and Anna Maria Vettraino and Caterina Villari and Yongjun Wang and Johanna Witzell and Milica Zlatković and René Eschen}, url = {https://www.nature.com/articles/s41598-023-36795-w}, doi = {10.1038/s41598-023-36795-w}, issn = {2045-2322}, year = {2023}, date = {2023-07-18}, journal = {Scientific Reports}, volume = {13}, pages = {11570 }, abstract = {Non-native pests, climate change, and their interactions are likely to alter relationships between trees and tree-associated organisms with consequences for forest health. To understand and predict such changes, factors structuring tree-associated communities need to be determined. Here, we analysed the data consisting of records of insects and fungi collected from dormant twigs from 155 tree species at 51 botanical gardens or arboreta in 32 countries. Generalized dissimilarity models revealed similar relative importance of studied climatic, host-related and geographic factors on differences in tree-associated communities. Mean annual temperature, phylogenetic distance between hosts and geographic distance between locations were the major drivers of dissimilarities. The increasing importance of high temperatures on differences in studied communities indicate that climate change could affect tree-associated organisms directly and indirectly through host range shifts. Insect and fungal communities were more similar between closely related vs. distant hosts suggesting that host range shifts may facilitate the emergence of new pests. Moreover, dissimilarities among tree-associated communities increased with geographic distance indicating that human-mediated transport may serve as a pathway of the introductions of new pests. The results of this study highlight the need to limit the establishment of tree pests and increase the resilience of forest ecosystems to changes in climate.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Non-native pests, climate change, and their interactions are likely to alter relationships between trees and tree-associated organisms with consequences for forest health. To understand and predict such changes, factors structuring tree-associated communities need to be determined. Here, we analysed the data consisting of records of insects and fungi collected from dormant twigs from 155 tree species at 51 botanical gardens or arboreta in 32 countries. Generalized dissimilarity models revealed similar relative importance of studied climatic, host-related and geographic factors on differences in tree-associated communities. Mean annual temperature, phylogenetic distance between hosts and geographic distance between locations were the major drivers of dissimilarities. The increasing importance of high temperatures on differences in studied communities indicate that climate change could affect tree-associated organisms directly and indirectly through host range shifts. Insect and fungal communities were more similar between closely related vs. distant hosts suggesting that host range shifts may facilitate the emergence of new pests. Moreover, dissimilarities among tree-associated communities increased with geographic distance indicating that human-mediated transport may serve as a pathway of the introductions of new pests. The results of this study highlight the need to limit the establishment of tree pests and increase the resilience of forest ecosystems to changes in climate. |
Kádasi-Horáková, M; Barta, M; Adamčíková, K; Ostrovský, R; Pastirčáková, K Hymenoscyphus fraxineus on Fraxinus excelsior in Slovakia: distribution and mating types Journal Article Biologia, 78 (5), pp. 1219-1230, 2023, ISSN: 0006-3088. @article{Kádasi-Horáková2023, title = {\textit{Hymenoscyphus fraxineus} on \textit{Fraxinus excelsior} in Slovakia: distribution and mating types}, author = {M. Kádasi-Horáková and M. Barta and K. Adamčíková and R. Ostrovský and K. Pastirčáková}, doi = {10.1007/s11756-022-01023-9}, issn = {0006-3088}, year = {2023}, date = {2023-05-10}, journal = {Biologia}, volume = {78}, number = {5}, pages = {1219-1230}, abstract = {Hymenoscyphus fraxineus causes ash dieback in Europe and threatens the future existence of Fraxinus excelsior in large parts of its natural distribution range. In this study, we report the first documented distribution of the pathogen on the most common ash species F. excelsior in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region of rDNA. Analysis of the mating type genes of H. fraxineus isolates revealed the presence of both mating types in Slovakia. Hymenoscyphus fraxineus-positive trees were recorded in 70 localities with different types of management in different parts of the country, mainly in forest stands. The results indicate the widespread distribution of H. fraxineus across the entire country, wherever the host is present.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Hymenoscyphus fraxineus causes ash dieback in Europe and threatens the future existence of Fraxinus excelsior in large parts of its natural distribution range. In this study, we report the first documented distribution of the pathogen on the most common ash species F. excelsior in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region of rDNA. Analysis of the mating type genes of H. fraxineus isolates revealed the presence of both mating types in Slovakia. Hymenoscyphus fraxineus-positive trees were recorded in 70 localities with different types of management in different parts of the country, mainly in forest stands. The results indicate the widespread distribution of H. fraxineus across the entire country, wherever the host is present. |
Jánošíková, R; Adamčíková, K Development and changes in pathogens population causing Dothistroma needle blight in Pinus nigra plantation in Strážovské vrchy Mts Journal Article Biologia, 78 (5), pp. 1231-1237, 2023, ISSN: 1336-9563. @article{Jánošíková2023, title = {Development and changes in pathogens population causing Dothistroma needle blight in Pinus nigra plantation in Strážovské vrchy Mts}, author = {R. Jánošíková and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s11756-022-01135-2}, doi = {doi.org/10.1007/s11756-022-01135-2}, issn = {1336-9563}, year = {2023}, date = {2023-05-10}, journal = {Biologia}, volume = {78}, number = {5}, pages = {1231-1237}, abstract = {Dothistroma septosporum and Dothistroma pini are two species of ascomycetous fungi of the genus Dothistroma, which cause needle disease worldwide known as Dothistroma needle blight (DNB). DNB attacks needles mostly from the Pinus species and causes signifcant defoliation on their hosts. Both Dothistroma species produce identical symptoms, the only unambiguous method of distinguishing are molecular identifcation methods. PCR techniques using species and mating-type specifc primers were used to compare the development of the population structures of both fungal pathogens after 12 years. The basis for this comparative analysis was the biological material we collected from the P. nigra plantation in 2018 and the collection of 24 isolates from the same research area collected in 2006. From the whole number of 107 established cultures, we obtained 56 isolates, while the presence of both Dothistroma species was confrmed. However, 12 years ago only D. septosporum occurred at the research area. For the species, D. septosporum, we identifed both mating types with slight dominance of MAT1-2, but still not deviated from a 1:1 ratio (MAT1-1: MAT1-2=26:33), similar results were obtained within samples 12 years before, suggesting sexual reproduction occurs in the population. Whereas single isolate for the species D. pini was identifed as the mating type MAT1-1.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma septosporum and Dothistroma pini are two species of ascomycetous fungi of the genus Dothistroma, which cause needle disease worldwide known as Dothistroma needle blight (DNB). DNB attacks needles mostly from the Pinus species and causes signifcant defoliation on their hosts. Both Dothistroma species produce identical symptoms, the only unambiguous method of distinguishing are molecular identifcation methods. PCR techniques using species and mating-type specifc primers were used to compare the development of the population structures of both fungal pathogens after 12 years. The basis for this comparative analysis was the biological material we collected from the P. nigra plantation in 2018 and the collection of 24 isolates from the same research area collected in 2006. From the whole number of 107 established cultures, we obtained 56 isolates, while the presence of both Dothistroma species was confrmed. However, 12 years ago only D. septosporum occurred at the research area. For the species, D. septosporum, we identifed both mating types with slight dominance of MAT1-2, but still not deviated from a 1:1 ratio (MAT1-1: MAT1-2=26:33), similar results were obtained within samples 12 years before, suggesting sexual reproduction occurs in the population. Whereas single isolate for the species D. pini was identifed as the mating type MAT1-1. |
Ondrušková, E; Adamčík, S; Kobza, M; Jánošíková, Z; Ostrovský, R; Pastirčáková, K; Caboň, M; Adamčíková, K Checking the balance between pathogenic and mutualistic pine needle fungi of the genus Lophodermium in forested and urban areas of Slovakia Journal Article Scandinavian Journal of Forest Research, 38 (1-2), pp. 39-48, 2023, ISSN: 1651-1891. @article{Ondrušková2023, title = {Checking the balance between pathogenic and mutualistic pine needle fungi of the genus Lophodermium in forested and urban areas of Slovakia}, author = {E. Ondrušková and S. Adamčík and M. Kobza and Z. Jánošíková and R. Ostrovský and K. Pastirčáková and M. Caboň and K. Adamčíková }, doi = { DOI: 10.1080/02827581.2023.2191004}, issn = {1651-1891}, year = {2023}, date = {2023-03-27}, journal = {Scandinavian Journal of Forest Research}, volume = {38}, number = {1-2}, pages = {39-48}, abstract = {Lophodermium species are well known to be among dominant endophytic fungi colonising pine needles. Occurrence of two species with different life strategies on different Pinus trees across Slovakia was detected by PCR using species specific primers. In general, commensalistic species L. pinastri was more frequent than parasitic L. seditiosum. There were no significant differences observed between urban and natural environments, but natural environments have more frequent colonisation by L. pinastri and less frequent were trees negatively tested to Lophodermium colonisation. Among the most frequently sampled trees were non-native P. nigra and native P. sylvestris. Significant difference in Lophodermium incidence was detected between these two species in natural environment, with increased frequency of L. pinastri on native Scots pine. In addition to one clade of L. pinastri, culture based species identification confirmed presence of L. corconticum and an undescribed clade of L. seditiosum that is not sensitive to used PCR primers for the species detection.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Lophodermium species are well known to be among dominant endophytic fungi colonising pine needles. Occurrence of two species with different life strategies on different Pinus trees across Slovakia was detected by PCR using species specific primers. In general, commensalistic species L. pinastri was more frequent than parasitic L. seditiosum. There were no significant differences observed between urban and natural environments, but natural environments have more frequent colonisation by L. pinastri and less frequent were trees negatively tested to Lophodermium colonisation. Among the most frequently sampled trees were non-native P. nigra and native P. sylvestris. Significant difference in Lophodermium incidence was detected between these two species in natural environment, with increased frequency of L. pinastri on native Scots pine. In addition to one clade of L. pinastri, culture based species identification confirmed presence of L. corconticum and an undescribed clade of L. seditiosum that is not sensitive to used PCR primers for the species detection. |
Tubby, K; Adamčíková, K; Adamson, K; Akiba, M; Barnes, I; Boroń, P; Bragança, H; Bulgakov, T; Burgdorf, N; Capretti, P; Cech, T; Cleary, M; Davydenko, K; Drenkhan, R; Elvira-Recuenco, M; Enderle, R; Gardner, J; Georgieva, M; Ghelardini, L; Husson, C; Iturritxa, E; Markovskaja, S; Mesanza, N; Ogris, N; Oskay, F; Piškur, B; Queloz, V; Raitelaityte, K; Raposo, R; Soukainen, M; Strasser, L; Vahalík, P; Vester, M; Mullett, M The increasing threat to European forests from the invasive foliar pine pathogen, Lecanosticta acicola Journal Article Forets Ecology and Management, 536 , pp. 120847, 2023. @article{Tubby2023, title = {The increasing threat to European forests from the invasive foliar pine pathogen, Lecanosticta acicola}, author = {K. Tubby and K. Adamčíková and K. Adamson and M. Akiba and I. Barnes and P. Boroń and H. Bragança and T. Bulgakov and N. Burgdorf and P. Capretti and T. Cech and M. Cleary and K. Davydenko and R. Drenkhan and M. Elvira-Recuenco and R. Enderle and J. Gardner and M. Georgieva and L. Ghelardini and C. Husson and E. Iturritxa and S. Markovskaja and N. Mesanza and N. Ogris and F. Oskay and B. Piškur and V. Queloz and K. Raitelaityte and R. Raposo and M. Soukainen and L. Strasser and P. Vahalík and M. Vester and M. Mullett}, doi = {doi.org/10.1016/j.foreco.2023.120847 }, year = {2023}, date = {2023-03-23}, journal = {Forets Ecology and Management}, volume = {536}, pages = { 120847}, abstract = {European forests are threatened by increasing numbers of invasive pests and pathogens. Over the past century, Lecanosticta acicola, a foliar pathogen predominantly of Pinus spp., has expanded its range globally, and is increasing in impact. Lecanosticta acicola causes brown spot needle blight, resulting in premature defoliation, reduced growth, and mortality in some hosts. Originating from southern regions of North American, it devastated forests in the USA’s southern states in the early twentieth century, and in 1942 was discovered in Spain. Derived from Euphresco project Brownspotrisk,’ this study aimed to establish the current distribution of Lecanosticta species, and assess the risks of L. acicola to European forests. Pathogen reports from the literature, and new/ unpublished survey data were combined into an open-access geo-database (http://www.portaloff orestpathology.com), and used to visualise the pathogen’s range, infer its climatic tolerance, and update its host range. Lecanosticta species have now been recorded in 44 countries, mostly in the northern hemisphere. The type species, L. acicola, has increased its range in recent years, and is present in 24 out of the 26 European countries where data were available. Other species of Lecanosticta are largely restricted to Mexico and Central America, and recently Colombia. The geo-database records demonstrate that L. acicola tolerates a wide range of climates across the northern hemisphere, and indicate its potential to colonise Pinus spp. forests across large swathes of the Europe. Preliminary analyses suggest L. acicola could affect 62% of global Pinus species area by the end of this century, under climate change predictions. Although its host range appears slightly narrower than the similar Dothistroma species, Lecanosticta species were recorded on 70 host taxa, mostly Pinus spp., but including, Cedrus and Picea spp. Twenty-three, including species of critical ecological, environmental and economic significance in Europe, are highly susceptible to L. acicola, suffering heavy defoliation and sometimes mortality. Variation in apparent susceptibility between reports could reflect variation between regions in the hosts’ genetic make-up, but could also reflect the significant variation in L. acicola populations and lineages found across Europe. This study served to highlight significant gaps in our understanding of the pathogen’s behaviour. Lecanosticta acicola has recently been downgraded from an A1 quarantine pest to a regulated non quarantine pathogen, and is now widely distributed across Europe. With a need to consider disease management, this study also explored global BSNB strategies, and used Case Studies to summarise the tactics employed to date in Europe. }, keywords = {}, pubstate = {published}, tppubtype = {article} } European forests are threatened by increasing numbers of invasive pests and pathogens. Over the past century, Lecanosticta acicola, a foliar pathogen predominantly of Pinus spp., has expanded its range globally, and is increasing in impact. Lecanosticta acicola causes brown spot needle blight, resulting in premature defoliation, reduced growth, and mortality in some hosts. Originating from southern regions of North American, it devastated forests in the USA’s southern states in the early twentieth century, and in 1942 was discovered in Spain. Derived from Euphresco project Brownspotrisk,’ this study aimed to establish the current distribution of Lecanosticta species, and assess the risks of L. acicola to European forests. Pathogen reports from the literature, and new/ unpublished survey data were combined into an open-access geo-database (http://www.portaloff orestpathology.com), and used to visualise the pathogen’s range, infer its climatic tolerance, and update its host range. Lecanosticta species have now been recorded in 44 countries, mostly in the northern hemisphere. The type species, L. acicola, has increased its range in recent years, and is present in 24 out of the 26 European countries where data were available. Other species of Lecanosticta are largely restricted to Mexico and Central America, and recently Colombia. The geo-database records demonstrate that L. acicola tolerates a wide range of climates across the northern hemisphere, and indicate its potential to colonise Pinus spp. forests across large swathes of the Europe. Preliminary analyses suggest L. acicola could affect 62% of global Pinus species area by the end of this century, under climate change predictions. Although its host range appears slightly narrower than the similar Dothistroma species, Lecanosticta species were recorded on 70 host taxa, mostly Pinus spp., but including, Cedrus and Picea spp. Twenty-three, including species of critical ecological, environmental and economic significance in Europe, are highly susceptible to L. acicola, suffering heavy defoliation and sometimes mortality. Variation in apparent susceptibility between reports could reflect variation between regions in the hosts’ genetic make-up, but could also reflect the significant variation in L. acicola populations and lineages found across Europe. This study served to highlight significant gaps in our understanding of the pathogen’s behaviour. Lecanosticta acicola has recently been downgraded from an A1 quarantine pest to a regulated non quarantine pathogen, and is now widely distributed across Europe. With a need to consider disease management, this study also explored global BSNB strategies, and used Case Studies to summarise the tactics employed to date in Europe. |
van der Nest, A; Wingfield, M J; Sadiković, D; Mullett, M S; Marçais, B; Queloz, V; Adamčíková, K; Davydenko, K; Barnes, I Population structure and diversity of the needle pathogen Dothistroma pini suggests human-mediated movement in Europe Journal Article Frontiers in Genetics, 14 , pp. 1103331, 2023, ISSN: 1338-7014. @article{Bičárová2023b, title = {Population structure and diversity of the needle pathogen Dothistroma pini suggests human-mediated movement in Europe}, author = {A. van der Nest and M. J. Wingfield and D. Sadiković and M. S. Mullett and B. Marçais and V. Queloz and K. Adamčíková and K. Davydenko and I. Barnes}, doi = {doi: 10.3389/fgene.2023.1103331}, issn = {1338-7014}, year = {2023}, date = {2023-02-16}, journal = {Frontiers in Genetics}, volume = {14}, pages = {1103331}, abstract = {Dothistroma needle blight (DNB) is an important disease of Pinus species that can be caused by one of two distinct but closely related pathogens; Dothistroma septosporum and Dothistroma pini. Dothistroma septosporum has a wide geographic distribution and is relatively well-known. In contrast, D. pini is known only from the United States and Europe, and there is a distinct lack of knowledge regarding its population structure and genetic diversity. The recent development of 16 microsatellite markers for D. pini provided an opportunity to investigate the diversity, structure, and mode of reproduction for populations collected over a period of 12 years, on eight different hosts in Europe. In total, 345 isolates from Belgium, the Czech Republic, France, Hungary, Romania, Western Russia, Serbia, Slovakia, Slovenia, Spain, Switzerland, and Ukraine were screened using microsatellite and species-specific mating type markers. A total of 109 unique multilocus haplotypes were identified and structure analyses suggested that the populations are influenced by location rather than host species. Populations from France and Spain displayed the highest levels of genetic diversity followed by the population in Ukraine. Both mating types were detected in most countries, with the exception of Hungary, Russia and Slovenia. Evidence for sexual recombination was supported only in the population from Spain. The observed population structure and several shared haplotypes between non-bordering countries provides good evidence that the movement of D. pini in Europe has been strongly influenced by human activity in Europe.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is an important disease of Pinus species that can be caused by one of two distinct but closely related pathogens; Dothistroma septosporum and Dothistroma pini. Dothistroma septosporum has a wide geographic distribution and is relatively well-known. In contrast, D. pini is known only from the United States and Europe, and there is a distinct lack of knowledge regarding its population structure and genetic diversity. The recent development of 16 microsatellite markers for D. pini provided an opportunity to investigate the diversity, structure, and mode of reproduction for populations collected over a period of 12 years, on eight different hosts in Europe. In total, 345 isolates from Belgium, the Czech Republic, France, Hungary, Romania, Western Russia, Serbia, Slovakia, Slovenia, Spain, Switzerland, and Ukraine were screened using microsatellite and species-specific mating type markers. A total of 109 unique multilocus haplotypes were identified and structure analyses suggested that the populations are influenced by location rather than host species. Populations from France and Spain displayed the highest levels of genetic diversity followed by the population in Ukraine. Both mating types were detected in most countries, with the exception of Hungary, Russia and Slovenia. Evidence for sexual recombination was supported only in the population from Spain. The observed population structure and several shared haplotypes between non-bordering countries provides good evidence that the movement of D. pini in Europe has been strongly influenced by human activity in Europe. |
Bičárová, Svetlana; Lukasová, Veronika; Adamčíková, Katarína; Žatková, Lucia; Milovský, Rastislav; Shashikumar, Anumol; Pažitný, Jozef; Buchholcerová, Anna; Bilčík, Dušan Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress Journal Article Folia Oecologica, 50 (1), pp. 1 - 15, 2023. @article{Bičárová2023, title = {Modified electrolyte leakage method for testing the oxidative stability of \textit{Pinus mugo} Turra under ozone-induced stress}, author = {Svetlana Bičárová and Veronika Lukasová and Katarína Adamčíková and Lucia Žatková and Rastislav Milovský and Anumol Shashikumar and Jozef Pažitný and Anna Buchholcerová and Dušan Bilčík}, url = {http://ife.sk/wp-content/uploads/2020/09/foecol-2023-0001.pdf}, doi = {10.2478/foecol-2023-0001}, year = {2023}, date = {2023-01-27}, journal = {Folia Oecologica}, volume = {50}, number = {1}, pages = {1 - 15}, abstract = {Electrolyte leakage (EL) is the method commonly used to test the cell membrane integrity of plants under stress conditions. The cells of the leaf may be damaged by ozone (O3) entering the intercellular space as an oxidative stress agent. The modified EL method was used to test the oxidative stability (OxS) of plant tissue against O3-induced oxidative stress. The modification includes simulation of the artificial oxidative stress by additional ozonation of plant samples in the laboratory chamber. This modified EL method was applied to Pinus mugo Turra needle samples collected in the subalpine zone of the High Tatra Mts (Western Carpathians), in the years 2019 and 2020. Changes in the chemical composition of samples after artificial ozonation were traced by gas chromatography/mass spectrometry (GC/MS) analysis. In addition, O3 uptake through open stomata was estimated by calculation of the modelled ozone dose (MO3D). We also conducted an inspection of visible injury (VIN) on the needle surface focused on the occurrence of O3-induced symptoms and biotic harmful agents. Regarding OxS results as well as VIN indices, P. mugo needles showed relatively low sensitivity to oxidative stress induced by O3. Therefore MO3D in a range between 14 and 16 mmol m−2 can be considered as O3 dose with minor phytotoxic effect on P. mugo growing in the mountains of central-eastern Europe.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Electrolyte leakage (EL) is the method commonly used to test the cell membrane integrity of plants under stress conditions. The cells of the leaf may be damaged by ozone (O3) entering the intercellular space as an oxidative stress agent. The modified EL method was used to test the oxidative stability (OxS) of plant tissue against O3-induced oxidative stress. The modification includes simulation of the artificial oxidative stress by additional ozonation of plant samples in the laboratory chamber. This modified EL method was applied to Pinus mugo Turra needle samples collected in the subalpine zone of the High Tatra Mts (Western Carpathians), in the years 2019 and 2020. Changes in the chemical composition of samples after artificial ozonation were traced by gas chromatography/mass spectrometry (GC/MS) analysis. In addition, O3 uptake through open stomata was estimated by calculation of the modelled ozone dose (MO3D). We also conducted an inspection of visible injury (VIN) on the needle surface focused on the occurrence of O3-induced symptoms and biotic harmful agents. Regarding OxS results as well as VIN indices, P. mugo needles showed relatively low sensitivity to oxidative stress induced by O3. Therefore MO3D in a range between 14 and 16 mmol m−2 can be considered as O3 dose with minor phytotoxic effect on P. mugo growing in the mountains of central-eastern Europe. |
2022 |
Laas, M; Adamson, K; Barnes, I; Janoušek, J; Mullett, M S; Adamčíková, K; Akiba, M; Beenken, L; Braganca, H; Bulgakov, T S; Capretti, P; Cech, T; Cleary, M; Enderle, R; Ghelardini, L; Jankovský, L; Markovskaja, S; Matsiakh, I; Meyer, J B; Oskay, F; Piškur, B; Raitelaitytė, K; Sadiković, D; Drenkhan, R Diversity, migration routes, and worldwide population genetic structure of Lecanosticta acicola, the causal agent of brown spot needle blight Journal Article Molecular Plant Pathology, 23 (11), pp. 1620-1639, 2022, ISSN: 1364-3703. @article{Laas2022, title = {Diversity, migration routes, and worldwide population genetic structure of Lecanosticta acicola, the causal agent of brown spot needle blight}, author = {M. Laas and K. Adamson and I. Barnes and J. Janoušek and M.S. Mullett and K. Adamčíková and M. Akiba and L. Beenken and H. Braganca and T.S. Bulgakov and P. Capretti and T. Cech and M. Cleary and R. Enderle and L. Ghelardini and L. Jankovský and S. Markovskaja and I. Matsiakh and J.B. Meyer and F. Oskay and B. Piškur and K. Raitelaitytė and D. Sadiković and R. Drenkhan}, url = {http://ife.sk/wp-content/uploads/2020/09/Bez-názvu.tiff}, doi = {DOI: 10.1111/mpp.13257}, issn = {1364-3703}, year = {2022}, date = {2022-10-14}, journal = {Molecular Plant Pathology}, volume = {23}, number = {11}, pages = {1620-1639}, abstract = {Lecanosticta acicola is a pine needle pathogen causing brown spot needle blight that results in premature needle shedding with considerable damage described in North America, Europe, and Asia. Microsatellite and mating type markers were used to study the population genetics, migration history, and reproduction mode of the pathogen, based on a collection of 650 isolates from 27 countries and 26 hosts across the range of L. acicola. The presence of L. acicola in Georgia was confirmed in this study. Migration analyses indicate there have been several introduction events from North America into Europe. However, some of the source populations still appear to remain unknown. The populations in Croatia and western Asia appear to originate from genetically similar populations in North America. Intercontinental movement of the pathogen was reflected in an identical haplotype occurring on two continents, in North America (Canada) and Europe (Germany). Several shared haplotypes between European populations further suggests more local pathogen movement between countries. Moreover, migration analyses indicate that the populations in northern Europe originate from more established populations in central Europe. Overall, the highest genetic diversity was observed in south-eastern USA. In Europe, the highest diversity was observed in France, where the presence of both known pathogen lineages was recorded. Less than half of the observed populations contained mating types in equal proportions. Although there is evidence of some sexual reproduction taking place, the pathogen spreads predominantly asexually and through anthropogenic activity.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Lecanosticta acicola is a pine needle pathogen causing brown spot needle blight that results in premature needle shedding with considerable damage described in North America, Europe, and Asia. Microsatellite and mating type markers were used to study the population genetics, migration history, and reproduction mode of the pathogen, based on a collection of 650 isolates from 27 countries and 26 hosts across the range of L. acicola. The presence of L. acicola in Georgia was confirmed in this study. Migration analyses indicate there have been several introduction events from North America into Europe. However, some of the source populations still appear to remain unknown. The populations in Croatia and western Asia appear to originate from genetically similar populations in North America. Intercontinental movement of the pathogen was reflected in an identical haplotype occurring on two continents, in North America (Canada) and Europe (Germany). Several shared haplotypes between European populations further suggests more local pathogen movement between countries. Moreover, migration analyses indicate that the populations in northern Europe originate from more established populations in central Europe. Overall, the highest genetic diversity was observed in south-eastern USA. In Europe, the highest diversity was observed in France, where the presence of both known pathogen lineages was recorded. Less than half of the observed populations contained mating types in equal proportions. Although there is evidence of some sexual reproduction taking place, the pathogen spreads predominantly asexually and through anthropogenic activity. |
Lukasová, V; Bičárová, S; Buchholcerová, A; Adamčíková, K Low sensitivity of Pinus mugo to surface ozone pollution in the subalpine zone of continental Europe Journal Article International Journal of Biometeorology, 66 (11), pp. 2311–2324, 2022, ISSN: 0020-7128. @article{Lukasová2022, title = {Low sensitivity of Pinus mugo to surface ozone pollution in the subalpine zone of continental Europe}, author = {V. Lukasová and S. Bičárová and A. Buchholcerová and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s00484-022-02359-2}, doi = {10.1007/s00484-022-02359-2}, issn = {0020-7128}, year = {2022}, date = {2022-09-15}, journal = {International Journal of Biometeorology}, volume = {66}, number = {11}, pages = {2311–2324}, abstract = {High altitudes have been exposed to enhanced levels of surface ozone (O3) concentrations over recent decades compared to the pre-industrial era. The responses of vegetation to this toxic pollutant are species-specifc and depend on the climate conditions. In this paper, we explored the reaction of Pinus mugo (P. mugo) to O3-induced stress in the continental climate of an ozone-rich mountain area in the High Tatra Mountains (Western Carpathians). The efects of O3 doses modelled by a deposition model, O3 concentrations and other factors on P. mugo were identifed from (a) satellite-based data via NDVI (normalised diferenced vegetation index) over 2000–2020 and (b) visible injury on needle samples gathered from P. mugo individuals at ground-truth sites in 2019 and 2020. Analysing the NDVI trend, we observed non-signifcant changes (p>0.05) in the greenness of P. mugo despite growing in an environment with the average seasonal O3 concentration around 51.6 ppbv, the maximum hourly concentrations more than 90 ppbv and increasing trend of O3 doses by 0.1 mmol m−2 PLA (plant leaf area) year−1. The visible O3 injury of samples collected at study sites was low (mean injury observed on 1–10% of needles’ surface), and the symptoms of injury caused by other biotic and abiotic factors prevailed over those caused by O3. In addition, the correlation analyses between NDVI and the climatic factors indicated a signifcant (p<0.05) and positive relationship with photosynthetic active radiation (R=0.45) in July, and with stomatal conductance (R=0.52) and temperature factor (R=0.43) in August. Therefore, we concluded that the positive efect of climate conditions, which support the growth processes of P. mugo, may suppress the negative efect of the mean O3 doses of 17.8 mmol m−2 PLA accumulated over the growing season.}, keywords = {}, pubstate = {published}, tppubtype = {article} } High altitudes have been exposed to enhanced levels of surface ozone (O3) concentrations over recent decades compared to the pre-industrial era. The responses of vegetation to this toxic pollutant are species-specifc and depend on the climate conditions. In this paper, we explored the reaction of Pinus mugo (P. mugo) to O3-induced stress in the continental climate of an ozone-rich mountain area in the High Tatra Mountains (Western Carpathians). The efects of O3 doses modelled by a deposition model, O3 concentrations and other factors on P. mugo were identifed from (a) satellite-based data via NDVI (normalised diferenced vegetation index) over 2000–2020 and (b) visible injury on needle samples gathered from P. mugo individuals at ground-truth sites in 2019 and 2020. Analysing the NDVI trend, we observed non-signifcant changes (p>0.05) in the greenness of P. mugo despite growing in an environment with the average seasonal O3 concentration around 51.6 ppbv, the maximum hourly concentrations more than 90 ppbv and increasing trend of O3 doses by 0.1 mmol m−2 PLA (plant leaf area) year−1. The visible O3 injury of samples collected at study sites was low (mean injury observed on 1–10% of needles’ surface), and the symptoms of injury caused by other biotic and abiotic factors prevailed over those caused by O3. In addition, the correlation analyses between NDVI and the climatic factors indicated a signifcant (p<0.05) and positive relationship with photosynthetic active radiation (R=0.45) in July, and with stomatal conductance (R=0.52) and temperature factor (R=0.43) in August. Therefore, we concluded that the positive efect of climate conditions, which support the growth processes of P. mugo, may suppress the negative efect of the mean O3 doses of 17.8 mmol m−2 PLA accumulated over the growing season. |
Barta, M; Pastirčáková, K; Ostrovský, R; Kobza, M; Kádasi-Horáková, M Culturable endophytic fungi in Fraxinus excelsior and their interactions with Hymenoscyphus fraxineus Journal Article Forests, 13 (7), pp. 1-23, Article no. 1098, 2022, ISSN: 1999-4907. @article{Barta2022b, title = {Culturable endophytic fungi in \textit{Fraxinus excelsior} and their interactions with \textit{Hymenoscyphus fraxineus}}, author = {M. Barta and K. Pastirčáková and R. Ostrovský and M. Kobza and M. Kádasi-Horáková}, doi = {10.3390/f13071098}, issn = {1999-4907}, year = {2022}, date = {2022-07-13}, journal = {Forests}, volume = {13}, number = {7}, pages = {1-23, Article no. 1098}, abstract = {The species diversity of culturable endophytic fungi was studied in the leaves and twigs of symptomatic and asymptomatic Fraxinus excelsior trees. Endophytic mycobiota was dominated by Ascomycota species, with Pleosporales (44.17%) and Diaporthales (23.79%) endophytes being the most frequently observed in the tree samples. The number of endophytic isolates and species richness varied depending on the sampling date (May and October) and tissue location. Of the 54 species identified based on ITS sequences, 14 were classified as dominant. The most frequently isolated species were Diaporthe eres, followed by Alternaria alternata, Dothiorella gregaria, and Fraxinicola fraxini. The inhibitory effect of 41 species (75 isolates) of endophytes on the radial growth of a Hymenoscyphus fraxineus isolate was studied under in vitro conditions (dual cultures). The radial growth of H. fraxineus was the most inhibited by four endophytic fungi from twigs (Fusarium lateritium, Didymella aliena, Didymella macrostoma, and Dothiorella gregaria). The inhibitory effect of the four isolates was also studied under in planta conditions. The isolates artificially inoculated into the trunks of ash trees reduced the length of necroses formed by H. fraxineus co-inoculated in the same trunks. This effect depended on the isolate, and the inhibition was most prominent only on trunks inoculated with F. lateritium and D. aliena. Although the total length of necrotic lesions formed by the H. fraxineus infection was shorter in the ash trunks co-inoculated with the endophytes, the difference was not significant.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The species diversity of culturable endophytic fungi was studied in the leaves and twigs of symptomatic and asymptomatic Fraxinus excelsior trees. Endophytic mycobiota was dominated by Ascomycota species, with Pleosporales (44.17%) and Diaporthales (23.79%) endophytes being the most frequently observed in the tree samples. The number of endophytic isolates and species richness varied depending on the sampling date (May and October) and tissue location. Of the 54 species identified based on ITS sequences, 14 were classified as dominant. The most frequently isolated species were Diaporthe eres, followed by Alternaria alternata, Dothiorella gregaria, and Fraxinicola fraxini. The inhibitory effect of 41 species (75 isolates) of endophytes on the radial growth of a Hymenoscyphus fraxineus isolate was studied under in vitro conditions (dual cultures). The radial growth of H. fraxineus was the most inhibited by four endophytic fungi from twigs (Fusarium lateritium, Didymella aliena, Didymella macrostoma, and Dothiorella gregaria). The inhibitory effect of the four isolates was also studied under in planta conditions. The isolates artificially inoculated into the trunks of ash trees reduced the length of necroses formed by H. fraxineus co-inoculated in the same trunks. This effect depended on the isolate, and the inhibition was most prominent only on trunks inoculated with F. lateritium and D. aliena. Although the total length of necrotic lesions formed by the H. fraxineus infection was shorter in the ash trunks co-inoculated with the endophytes, the difference was not significant. |
Barta, Marek; Semelbauer, Marek; Mangová, Barbara; Kozánek, Milan Entomopathogenic fungi associated with Stomoxys calcitrans in Slovakia and efficacy of local fungal strains against the stable fly Journal Article Acta fytotechnica et zootechnica, 25 (2), pp. 97-108, 2022, ISSN: ISSN 1336-9245. @article{Barta2022, title = {Entomopathogenic fungi associated with Stomoxys calcitrans in Slovakia and efficacy of local fungal strains against the stable fly}, author = {Marek Barta and Marek Semelbauer and Barbara Mangová and Milan Kozánek}, doi = {https://doi.org/10.15414/afz.2022.25.02.97-108}, issn = {ISSN 1336-9245}, year = {2022}, date = {2022-06-30}, journal = {Acta fytotechnica et zootechnica}, volume = {25}, number = {2}, pages = {97-108}, abstract = {The stable fly, Stomoxys calcitrans, is the most important ectoparasite of livestock throughout the world. Entomopathogenic fungi are natural antagonists of stable flies with the potential to be used as biocontrol agents in integrated management programmes of this pest. The prevalence and species diversity of entomopathogenic fungi in stable fly populations were documented on a dairy cattle farm in south-western Slovakia. The prevalence of fungal infection was low and the maximum percentage of infected flies reached 2.72% during May–October in 2016. Three Beauveria species were identified from infected flies, B. bassiana, B. pseudobassiana and B. brongniartii. Beauveria pseudobassiana and B. brongniartii are reported from S. calcitrans for the first time. In laboratory tests, the pathogenicity of four entomopathogenic fungi B. bassiana, B. pseudobassiana, M. anisopliae and Isaria fumosorosea against adult stable flies was compared. The flies were susceptible to infection by the fungi and the most virulent isolate of B. bassiana (IKEP10) was selected for spore formulation testing and field trials. We tested three types of spore formulations and the aqueous spore suspension was more effective against stable flies than spores formulated in the powder or oil suspension. Repeated treatments of the interior of the stable with B. bassiana spores at fortnightly intervals during June–August resulted in a 10 times higher prevalence of fungal infection in the stable fly population compared with the untreated stable, but the treatments did not effectively reduce the fly population. The selected B. bassiana isolate may represent an interesting tool in the biocontrol of stable fly populations, but further experiments are needed to design an appropriate formulation and enhance its efficacy.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The stable fly, Stomoxys calcitrans, is the most important ectoparasite of livestock throughout the world. Entomopathogenic fungi are natural antagonists of stable flies with the potential to be used as biocontrol agents in integrated management programmes of this pest. The prevalence and species diversity of entomopathogenic fungi in stable fly populations were documented on a dairy cattle farm in south-western Slovakia. The prevalence of fungal infection was low and the maximum percentage of infected flies reached 2.72% during May–October in 2016. Three Beauveria species were identified from infected flies, B. bassiana, B. pseudobassiana and B. brongniartii. Beauveria pseudobassiana and B. brongniartii are reported from S. calcitrans for the first time. In laboratory tests, the pathogenicity of four entomopathogenic fungi B. bassiana, B. pseudobassiana, M. anisopliae and Isaria fumosorosea against adult stable flies was compared. The flies were susceptible to infection by the fungi and the most virulent isolate of B. bassiana (IKEP10) was selected for spore formulation testing and field trials. We tested three types of spore formulations and the aqueous spore suspension was more effective against stable flies than spores formulated in the powder or oil suspension. Repeated treatments of the interior of the stable with B. bassiana spores at fortnightly intervals during June–August resulted in a 10 times higher prevalence of fungal infection in the stable fly population compared with the untreated stable, but the treatments did not effectively reduce the fly population. The selected B. bassiana isolate may represent an interesting tool in the biocontrol of stable fly populations, but further experiments are needed to design an appropriate formulation and enhance its efficacy. |
Galko, Juraj; Lalík, Michal; Rell, Slavomír; Nikolov, Christo; Barta, Marek; Pittner, Ján; Hyblerová, Silvia; Zúbrik, Milan; Kunca, Andrej; Vakula, Jozef; Gubka, Andrej; Holuša, Jaroslav Comprehensive comparison of treatments for controlling the large pine weevil (Hylobius abietis) in Central Europe Journal Article Scientific Reports, 12 (9673), pp. 1-15, 2022, ISBN: 2045-2322. @article{Galko2022, title = {Comprehensive comparison of treatments for controlling the large pine weevil (Hylobius abietis) in Central Europe}, author = {Juraj Galko and Michal Lalík and Slavomír Rell and Christo Nikolov and Marek Barta and Ján Pittner and Silvia Hyblerová and Milan Zúbrik and Andrej Kunca and Jozef Vakula and Andrej Gubka and Jaroslav Holuša}, doi = {https://doi.org/10.1038/s41598-022-13729-6}, isbn = {2045-2322}, year = {2022}, date = {2022-06-11}, journal = {Scientific Reports}, volume = {12}, number = {9673}, pages = {1-15}, abstract = {Adults of the large pine weevil (Hylobius abietis) cause serious damage to coniferous seedlings and are among the most important forest pests in Europe. Seedling protection by chemicals is gradually being restricted or banned for environmental reasons, and non-chemical alternatives are therefore needed. In this 3-year study, we compared the following five treatments for protecting Norway spruce seedlings against H. abietis in the Central European mountains where the weevil is especially abundant: alpha-cypermethrin sprays (the only chemical treatment); coating with sprayed glue (Vermifix); wax coating with C and F types (Norsk Wax); and physical protection with collars. The same block design was set up at a clear-cut site and at a nursery site to compare seedling mortality and wax quality under “wild conditions” with pests and under “ideal conditions” without pests. Repeated application of alpha-cypermethrin was the most effective and least expensive method to protect seedlings against H. abietis. Among the four non-chemical methods, repeated application of glue was the most effective. Because collars were moderately effective but not cost-effective, we do not recommend the use of collars. Wax was inexpensive and environmentally safe but protected seedlings for only 1 year; the newer F type of wax performed better than the C type of wax, and perhaps the F type can be improved. In general we found that seedlings at sites with high numbers of H. abietis require protection for at least 3 years. We conclude by providing an overview of all methods currently available for managing H. abietis in forests.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Adults of the large pine weevil (Hylobius abietis) cause serious damage to coniferous seedlings and are among the most important forest pests in Europe. Seedling protection by chemicals is gradually being restricted or banned for environmental reasons, and non-chemical alternatives are therefore needed. In this 3-year study, we compared the following five treatments for protecting Norway spruce seedlings against H. abietis in the Central European mountains where the weevil is especially abundant: alpha-cypermethrin sprays (the only chemical treatment); coating with sprayed glue (Vermifix); wax coating with C and F types (Norsk Wax); and physical protection with collars. The same block design was set up at a clear-cut site and at a nursery site to compare seedling mortality and wax quality under “wild conditions” with pests and under “ideal conditions” without pests. Repeated application of alpha-cypermethrin was the most effective and least expensive method to protect seedlings against H. abietis. Among the four non-chemical methods, repeated application of glue was the most effective. Because collars were moderately effective but not cost-effective, we do not recommend the use of collars. Wax was inexpensive and environmentally safe but protected seedlings for only 1 year; the newer F type of wax performed better than the C type of wax, and perhaps the F type can be improved. In general we found that seedlings at sites with high numbers of H. abietis require protection for at least 3 years. We conclude by providing an overview of all methods currently available for managing H. abietis in forests. |
Jančovičová, S; Adamčíková, K; Caboň, M; Adamčík, S Phylogeny of Crepidotus applanatus Look-Alikes Reveals a Convergent Morphology Evolution and a New Species C. pini Journal Article Journal of Fungi, 8 (489), 2022, ISSN: 2309-608X. @article{Jančovičová2022, title = {Phylogeny of Crepidotus applanatus Look-Alikes Reveals a Convergent Morphology Evolution and a New Species C. pini}, author = {S. Jančovičová and K. Adamčíková and M. Caboň and S. Adamčík}, url = {http://ife.sk/wp-content/uploads/2020/09/figure-2-5.jpg}, doi = {doi.org/10.3390/jof8050489}, issn = { 2309-608X}, year = {2022}, date = {2022-05-07}, journal = {Journal of Fungi}, volume = {8}, number = {489}, abstract = {Crepidotus applanatus is known as a common wood inhabiting fungus widely distributed throughout the Northern Hemisphere. There have been contrasting opinions about the delimitation and taxonomic treatment of the similar species C. malachius. Our phylogeny did not support the close relationship of these two morphologically similar species and the grouping of collections labelled by both names within each phylogenetic species reflects unreliable species delimitations in the traditional literatures. Both species inhabit the wood of deciduous trees, and our morphological analysis identified the size of basidiospores as a significant difference between them. The collections from Pinus sylvestris are recognised as a new species, C. pini sp. nov., and its morphological identification requires a combination of both basidiospore and cheilocystidia characters.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Crepidotus applanatus is known as a common wood inhabiting fungus widely distributed throughout the Northern Hemisphere. There have been contrasting opinions about the delimitation and taxonomic treatment of the similar species C. malachius. Our phylogeny did not support the close relationship of these two morphologically similar species and the grouping of collections labelled by both names within each phylogenetic species reflects unreliable species delimitations in the traditional literatures. Both species inhabit the wood of deciduous trees, and our morphological analysis identified the size of basidiospores as a significant difference between them. The collections from Pinus sylvestris are recognised as a new species, C. pini sp. nov., and its morphological identification requires a combination of both basidiospore and cheilocystidia characters. |
Takov, Danail; Barta, Marek; Toshova, Teodora; Doychev, Danail; Pilarska, Daniela On the pathogenicity of Metarhizium pemphigi against Ips typographus L. Journal Article Proceedings of the Bulgarian Academy of Sciences, 75 (4), pp. 554-560, 2022, ISSN: 1310–1331. @article{Takov2022, title = {On the pathogenicity of Metarhizium pemphigi against Ips typographus L.}, author = {Danail Takov and Marek Barta and Teodora Toshova and Danail Doychev and Daniela Pilarska}, doi = {10.7546/CRABS.2022.04.10}, issn = {1310–1331}, year = {2022}, date = {2022-05-02}, journal = {Proceedings of the Bulgarian Academy of Sciences}, volume = {75}, number = {4}, pages = {554-560}, abstract = {Efficacy of entomopathogenic fungus Metarhizium pemphigi was evaluated against Ips typographus adults in a laboratory bioassay for the first time. A series of four conidial concentrations (2×104–2×107 conidia/ml) were used. The cumulative mortality caused by M. pemphigi varied between 75% and 100% ten days post-treatment, with LC50 value of 2.9×103 conidia/ml and LC90 value of 6.4×104 conidia/ml. The median lethal time (LT50) of treated beetles depended on conidial concentrations and ranged from 1.78 to 5.98 days. Pathogenicity of M. pemphigi was reported for the first time and the tested strain was found to be promising for further evaluation in terms of management options against the spruce bark beetle.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Efficacy of entomopathogenic fungus Metarhizium pemphigi was evaluated against Ips typographus adults in a laboratory bioassay for the first time. A series of four conidial concentrations (2×104–2×107 conidia/ml) were used. The cumulative mortality caused by M. pemphigi varied between 75% and 100% ten days post-treatment, with LC50 value of 2.9×103 conidia/ml and LC90 value of 6.4×104 conidia/ml. The median lethal time (LT50) of treated beetles depended on conidial concentrations and ranged from 1.78 to 5.98 days. Pathogenicity of M. pemphigi was reported for the first time and the tested strain was found to be promising for further evaluation in terms of management options against the spruce bark beetle. |
Toshova, Teodora; Velchev, Dimitar; Barta, Marek; Takov, Danail; Todorov, Ivaylo; Pilarska, Daniela; Tóth, Miklós; Berkov, Strahil; Nikolova, Milena Insecticide activity of Greek oregano essential oil and entomopathogenic fungus Metarhizium pemphigi against Diabrotica virgifera virgifera LeConte Journal Article Forthcoming Cereal Research Communications, Forthcoming, ISSN: 1788-9170. @article{Toshova2022, title = {Insecticide activity of Greek oregano essential oil and entomopathogenic fungus Metarhizium pemphigi against Diabrotica virgifera virgifera LeConte}, author = {Teodora Toshova and Dimitar Velchev and Marek Barta and Danail Takov and Ivaylo Todorov and Daniela Pilarska and Miklós Tóth and Strahil Berkov and Milena Nikolova}, doi = {10.1007/s42976-022-00266-1}, issn = {1788-9170}, year = {2022}, date = {2022-04-08}, journal = {Cereal Research Communications}, abstract = {The western corn rootworm (WCR), Diabrotica virgifera virgifera, is an invasive alien species and an important pest of maize in Europe. The insecticide potential of Greek oregano, Origanum vulgare subsp. hirtum, essential oil (EO) and the entomopathogenic fungus Metarhizium pemphigi were evaluated against WCR adults in laboratory conditions as alternative control agents. Pure undissolved Greek oregano EO applied at a volume of 3, 5 and 10 µl caused 97–100% mean corrected mortality of the test individuals with a rapid lethal effect. Treatment of WCR with four concentrations of EO (0.01, 0.1, 1 and 10 µl/ml) showed a strong concentration–time effect. Mortality rate increased with increasing concentration and exposure time. The median lethal concentration LC50 was 0.03 µl/ml. The time needed for Greek oregano EO to cause LT50 of D. v. virgifera ranged from 17.95 days for the lowest concentration (0.01 µl/ml) to 90 min for the highest concentration (10 µl/ml). Metarhizium pemphigi, which was tested in five conidia concentrations (2 × 103–2 × 107 conidia/ml), showed relatively lower effectiveness against D. v. virgifera adults. At the end of the bioassay (ten days post-treatment), the mean corrected mortality of the test specimens varied from 14 to 73%. LC50 was 3.3 × 106 conidia/ml. The results showed that Greek oregano EO had a bioinsecticidal effect against WCR adults at low concentrations and they are a basis for further investigations on the EO as an environmentally friendly alternative to chemical insecticides.}, keywords = {}, pubstate = {forthcoming}, tppubtype = {article} } The western corn rootworm (WCR), Diabrotica virgifera virgifera, is an invasive alien species and an important pest of maize in Europe. The insecticide potential of Greek oregano, Origanum vulgare subsp. hirtum, essential oil (EO) and the entomopathogenic fungus Metarhizium pemphigi were evaluated against WCR adults in laboratory conditions as alternative control agents. Pure undissolved Greek oregano EO applied at a volume of 3, 5 and 10 µl caused 97–100% mean corrected mortality of the test individuals with a rapid lethal effect. Treatment of WCR with four concentrations of EO (0.01, 0.1, 1 and 10 µl/ml) showed a strong concentration–time effect. Mortality rate increased with increasing concentration and exposure time. The median lethal concentration LC50 was 0.03 µl/ml. The time needed for Greek oregano EO to cause LT50 of D. v. virgifera ranged from 17.95 days for the lowest concentration (0.01 µl/ml) to 90 min for the highest concentration (10 µl/ml). Metarhizium pemphigi, which was tested in five conidia concentrations (2 × 103–2 × 107 conidia/ml), showed relatively lower effectiveness against D. v. virgifera adults. At the end of the bioassay (ten days post-treatment), the mean corrected mortality of the test specimens varied from 14 to 73%. LC50 was 3.3 × 106 conidia/ml. The results showed that Greek oregano EO had a bioinsecticidal effect against WCR adults at low concentrations and they are a basis for further investigations on the EO as an environmentally friendly alternative to chemical insecticides. |
Buyck, B; Eyssartier, G; Armada, F; Corrales, A; Hembrom, M E; Rossi, W; Bellanger, J M; Das, K; Dima, B; Ghosh, A; Noordeloos, M E; Parihar, A; Krisai-Greilhuber, I; M., Leonardi M; Manz, C; Vera, M; Vila, J; Adamčíková, K; Bizio, E; Caboň, M; Hampe, F; Piepenbring, M; Adamčík, S Fungal Biodiversity Profiles 111-120 Journal Article Cryptogamie. Mycologie, 43 (2), pp. 23-61, 2022, ISSN: 1776-100. @article{Buyck2022, title = {Fungal Biodiversity Profiles 111-120}, author = {B. Buyck and G. Eyssartier and F. Armada and A. Corrales and M.E. Hembrom and W. Rossi and J.M. Bellanger and K. Das and B. Dima and A. Ghosh and M.E. Noordeloos and A. Parihar and I. Krisai-Greilhuber and M. Leonardi M. and C. Manz and M. Vera and J. Vila and K. Adamčíková and E. Bizio and M. Caboň and F. Hampe and M. Piepenbring and S. Adamčík }, url = {https://doi.org/10.5252/cryptogamie-mycologie2022v43a2. http://cryptogamie.com/mycologie/43/2}, doi = {doi.org/10.5252/cryptogamie-mycologie2022v43a2. }, issn = { 1776-100}, year = {2022}, date = {2022-03-03}, journal = {Cryptogamie. Mycologie}, volume = {43}, number = {2}, pages = {23-61}, abstract = {In this new series of Fungal Biodiversity Profiles, the authors provide descriptions for ten new taxa: Ilytheomyces uncinatus W. Rossi & M. Leonardi, sp. nov. (Ascomycota, Laboulbeniales) and several Basidiomycota: in family Entolomataceae for Entoloma aurantioalpinum Armada, Vila, Bellanger, Noordel., Krisai & Dima, sp. nov. and E. nigroflavescens Armada, Bellanger, Noordel. & Dima, sp. nov., both from France; in family Inocybaceae for Inocybe hebes Eyssart. & Buyck, sp. nov., I. media Eyssart. & Buyck, sp. nov. and I. leucophaea Eyssart. & Buyck, sp. nov., all three gathered from miombo woodland in Zambia (Africa); in family Russulaceae, Russula ferruginea subsp. ferruginea Corrales & Vera, sp. nov. and subsp. panamanensis Corrales & Manz, subsp. nov. from Central America; in Laetiporaceae Jülich for the Indian Phaeolus sharmae Hembrom, A. Parihar, K. Das & A. Ghosh, sp. nov.; in family Vuilleminiaceae Maire for Vuilleminia tropica Hembrom, A. Ghosh, A. Parihar & K. Das, sp. nov., equally from India. All new species of Basidiomycota are supported by newly provided sequence data or already published phylogenetic analyses of sequence data.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In this new series of Fungal Biodiversity Profiles, the authors provide descriptions for ten new taxa: Ilytheomyces uncinatus W. Rossi & M. Leonardi, sp. nov. (Ascomycota, Laboulbeniales) and several Basidiomycota: in family Entolomataceae for Entoloma aurantioalpinum Armada, Vila, Bellanger, Noordel., Krisai & Dima, sp. nov. and E. nigroflavescens Armada, Bellanger, Noordel. & Dima, sp. nov., both from France; in family Inocybaceae for Inocybe hebes Eyssart. & Buyck, sp. nov., I. media Eyssart. & Buyck, sp. nov. and I. leucophaea Eyssart. & Buyck, sp. nov., all three gathered from miombo woodland in Zambia (Africa); in family Russulaceae, Russula ferruginea subsp. ferruginea Corrales & Vera, sp. nov. and subsp. panamanensis Corrales & Manz, subsp. nov. from Central America; in Laetiporaceae Jülich for the Indian Phaeolus sharmae Hembrom, A. Parihar, K. Das & A. Ghosh, sp. nov.; in family Vuilleminiaceae Maire for Vuilleminia tropica Hembrom, A. Ghosh, A. Parihar & K. Das, sp. nov., equally from India. All new species of Basidiomycota are supported by newly provided sequence data or already published phylogenetic analyses of sequence data. |
Franić, Iva; Prospero, Simone; Adamson, Kalev; Allan, Eric; Attorre, Fabio; Auger-Rozenberg, Marie Anne; Augustin, Sylvie; Avtzis, Dimitrios; Baert, Wim; Barta, Marek; Bauters, Kenneth; Bellahirech, Amani; Boroń, Piotr; Bragança, Helena; Brestovanská, Tereza; Brurberg, May Bente; Burgess, Treena; Burokienė, Daiva; Cleary, Michelle; Corley, Juan; Coyle, David R; Csóka, György; Černý, Karel; Davydenko, Kateryna; de Groot, Maarten; Diez, Julio Javier; Lehtijärvi, Tuğba Doğmuş H; Drenkhan, Rein; Edwards, Jacqueline; Elsafy, Mohammed; Eötvös, Csaba Béla; Falko, Roman; Fan, Jianting; Feddern, Nina; Fürjes-Mikó, Ágnes; Gossner, Martin M; Grad, Bartłomiej; Hartmann, Martin; Havrdova, Ludmila; Horáková, Miriam Kádasi; Hrabětová, Markéta; Justesen, Mathias Just; Kacprzyk, Magdalena; Kenis, Marc; Kirichenko, Natalia; Kovač, Marta; Kramarets, Volodymyr; Lacković, Nikola; Lantschner, Maria Victoria; Lazarević, Jelena; Leskiv, Marianna; Li, Hongmei; Madsen, Corrie Lynne; Malumphy, Chris; Matošević, Dinka; Matsiakh, Iryna; May, Tom W; Meffert, Johan; Migliorini, Duccio; Nikolov, Christo; O’Hanlon, Richard; Oskay, Funda; Paap, Trudy; Parpan, Taras; Piškur, Barbara; Ravn, Hans Peter; Richard, John; Ronse, Anne; Roques, Alain; Ruffner, Beat; Sivickis, Karolis; Soliani, Carolina; Talgø, Venche; Tomoshevich, Maria; Uimari, Anne; Ulyshen, Michael; Vettraino, Anna Maria; Villari, Caterina; Wang, Yongjun; Witzell, Johanna; Zlatković, Milica; Eschen, René Worldwide diversity of endophytic fungi and insects associated with dormant tree twigs Journal Article Scientific Data, 9 (62 (2022)), pp. 1-9, 2022, ISSN: 2052-4463. @article{Franić2022, title = {Worldwide diversity of endophytic fungi and insects associated with dormant tree twigs}, author = {Iva Franić and Simone Prospero and Kalev Adamson and Eric Allan and Fabio Attorre and Marie Anne Auger-Rozenberg and Sylvie Augustin and Dimitrios Avtzis and Wim Baert and Marek Barta and Kenneth Bauters and Amani Bellahirech and Piotr Boroń and Helena Bragança and Tereza Brestovanská and May Bente Brurberg and Treena Burgess and Daiva Burokienė and Michelle Cleary and Juan Corley and David R. Coyle and György Csóka and Karel Černý and Kateryna Davydenko and Maarten de Groot and Julio Javier Diez and H. Tuğba Doğmuş Lehtijärvi and Rein Drenkhan and Jacqueline Edwards and Mohammed Elsafy and Csaba Béla Eötvös and Roman Falko and Jianting Fan and Nina Feddern and Ágnes Fürjes-Mikó and Martin M. Gossner and Bartłomiej Grad and Martin Hartmann and Ludmila Havrdova and Miriam Kádasi Horáková and Markéta Hrabětová and Mathias Just Justesen and Magdalena Kacprzyk and Marc Kenis and Natalia Kirichenko and Marta Kovač and Volodymyr Kramarets and Nikola Lacković and Maria Victoria Lantschner and Jelena Lazarević and Marianna Leskiv and Hongmei Li and Corrie Lynne Madsen and Chris Malumphy and Dinka Matošević and Iryna Matsiakh and Tom W. May and Johan Meffert and Duccio Migliorini and Christo Nikolov and Richard O’Hanlon and Funda Oskay and Trudy Paap and Taras Parpan and Barbara Piškur and Hans Peter Ravn and John Richard and Anne Ronse and Alain Roques and Beat Ruffner and Karolis Sivickis and Carolina Soliani and Venche Talgø and Maria Tomoshevich and Anne Uimari and Michael Ulyshen and Anna Maria Vettraino and Caterina Villari and Yongjun Wang and Johanna Witzell and Milica Zlatković and René Eschen}, doi = {10.1038/s41597-022-01162-3}, issn = {2052-4463}, year = {2022}, date = {2022-03-01}, journal = {Scientific Data}, volume = {9}, number = {62 (2022)}, pages = {1-9}, abstract = {International trade in plants and climate change are two of the main factors causing damaging tree pests (i.e. fungi and insects) to spread into new areas. To mitigate these risks, a large-scale assessment of tree-associated fungi and insects is needed. We present records of endophytic fungi and insects in twigs of 17 angiosperm and gymnosperm genera, from 51 locations in 32 countries worldwide. Endophytic fungi were characterized by high-throughput sequencing of 352 samples from 145 tree species in 28 countries. Insects were reared from 227 samples of 109 tree species in 18 countries and sorted into taxonomic orders and feeding guilds. Herbivorous insects were grouped into morphospecies and were identified using molecular and morphological approaches. This dataset reveals the diversity of tree-associated taxa, as it contains 12,721 fungal Amplicon Sequence Variants and 208 herbivorous insect morphospecies, sampled across broad geographic and climatic gradients and for many tree species. This dataset will facilitate applied and fundamental studies on the distribution of fungal endophytes and insects in trees.}, keywords = {}, pubstate = {published}, tppubtype = {article} } International trade in plants and climate change are two of the main factors causing damaging tree pests (i.e. fungi and insects) to spread into new areas. To mitigate these risks, a large-scale assessment of tree-associated fungi and insects is needed. We present records of endophytic fungi and insects in twigs of 17 angiosperm and gymnosperm genera, from 51 locations in 32 countries worldwide. Endophytic fungi were characterized by high-throughput sequencing of 352 samples from 145 tree species in 28 countries. Insects were reared from 227 samples of 109 tree species in 18 countries and sorted into taxonomic orders and feeding guilds. Herbivorous insects were grouped into morphospecies and were identified using molecular and morphological approaches. This dataset reveals the diversity of tree-associated taxa, as it contains 12,721 fungal Amplicon Sequence Variants and 208 herbivorous insect morphospecies, sampled across broad geographic and climatic gradients and for many tree species. This dataset will facilitate applied and fundamental studies on the distribution of fungal endophytes and insects in trees. |
Kobza, M; Ostrovský, R; Adamčíková, K; Pastirčáková, K Stability of trees infected by wood decay fungi estimated by acoustic tomography: a field survey Journal Article Trees Structure and Function, 36 (1), pp. 103-112, 2022, ISSN: 1432-2285. @article{Kobza2022, title = {Stability of trees infected by wood decay fungi estimated by acoustic tomography: a field survey}, author = {M. Kobza and R. Ostrovský and K. Adamčíková and K. Pastirčáková}, url = {https://doi.org/10.1007/s00468-021-02185-w}, doi = {10.1007/s00468-021-02185-w}, issn = {1432-2285}, year = {2022}, date = {2022-02-08}, journal = {Trees Structure and Function}, volume = {36}, number = {1}, pages = {103-112}, abstract = {In this survey, we assessed the relationships between the presence of visible fruiting bodies and measurements of both wood damage and tree safety factor (SF), the final aim being the determination of whether a tree showing wood decay signs should be felled or not. In general, trees affected by wood-decaying fungi cannot be considered risky prior to determining the impact of the fungi on their structural integrity. Trees of nine genera from 13 localities of Slovakia were included in this survey. In total, nineteen species of fungi were found causing wood decay on 74 individual host trees. Acoustic tomography was done using a Fakopp 3D tomograph, and SF was calculated by ArborSonic 3D software. The most commonly occurring taxon, Fomes fomentarius (L.) Fr., was found on six tree genera. The highest degree of damaged area on trunks was caused by fungi in the genera Fomes, Ganoderma, and Perenniporia (over 70%), while the lowest damage was caused by genera Spongipellis, Cerrena, and Auricularia (up to 30%). The lowest values of SF were measured on trees of the genus Acer infected by Fomes fomentarius; Prunus infected by Phellinus pomaceus (Pers.) Maire; and Fagus infected by Ganoderma pfeifferi Bres. Computation of the SF of individual trees plays a vital part in the stability evaluation of trees affected by wood decay fungi.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In this survey, we assessed the relationships between the presence of visible fruiting bodies and measurements of both wood damage and tree safety factor (SF), the final aim being the determination of whether a tree showing wood decay signs should be felled or not. In general, trees affected by wood-decaying fungi cannot be considered risky prior to determining the impact of the fungi on their structural integrity. Trees of nine genera from 13 localities of Slovakia were included in this survey. In total, nineteen species of fungi were found causing wood decay on 74 individual host trees. Acoustic tomography was done using a Fakopp 3D tomograph, and SF was calculated by ArborSonic 3D software. The most commonly occurring taxon, Fomes fomentarius (L.) Fr., was found on six tree genera. The highest degree of damaged area on trunks was caused by fungi in the genera Fomes, Ganoderma, and Perenniporia (over 70%), while the lowest damage was caused by genera Spongipellis, Cerrena, and Auricularia (up to 30%). The lowest values of SF were measured on trees of the genus Acer infected by Fomes fomentarius; Prunus infected by Phellinus pomaceus (Pers.) Maire; and Fagus infected by Ganoderma pfeifferi Bres. Computation of the SF of individual trees plays a vital part in the stability evaluation of trees affected by wood decay fungi. |
Michalko, Jaroslav; Medo, Juraj; Ferus, Peter; Konôpková, Jana; Košútová, Dominika; Hot’ka, Peter; Barta, Marek Plants, 11 , pp. 417, 2022, ISSN: 2223-7747. @article{Michalko2022, title = {Changes of Endophytic Bacterial Community in Mature Leaves of Prunus laurocerasus L. during the Seasonal Transition from Winter Dormancy to Vegetative Growth}, author = {Jaroslav Michalko and Juraj Medo and Peter Ferus and Jana Konôpková and Dominika Košútová and Peter Hot’ka and Marek Barta}, doi = {10.3390/plants11030417}, issn = {2223-7747}, year = {2022}, date = {2022-02-03}, journal = {Plants}, volume = {11}, pages = {417}, abstract = {Diverse communities of bacterial endophytes inhabit plant tissues, and these bacteria play important roles for plant growth and health. Cherry laurel (Prunus laurocerasus L.) is a broadleaf evergreen shrub that is widely grown in temperate zones for its ornamental and medicinal properties, however virtually nothing is known about its associated bacterial community. In this study, we analysed the matured one-year-old leaves of this plant using Illumina-based 16S rRNA gene metabarcoding to reveal the community structure of endophytic bacteria and understand its shifts during the seasonal transition from winter dormancy to a spring vegetative state. The overall community was composed of four dominant phyla (Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes). Corynebacterium, Acinetobacter, and Chryseobacterium genera were the most prevalent bacteria, comprising 13.3%, 6.9%, and 6.8% of the amplicon sequence variants (ASVs), respectively. The ASV richness and diversity increased significantly in May as compared to other sampling months (February, March, and April). We observed high variation in the overall community structure of endophytic bacteria among collection dates. The variation was only reflected by a few core community members, suggesting that the changes of the endophytic community during winter/spring seasonal transition are mostly associated with the less abundant community members. We identified biomarker taxa for late winter, mid spring, and late spring collection dates. This study is the first one to report on the diversity and composition of bacterial endophytes in the leaves of cherry laurel and its shifts across the dormancy-to-vegetative seasonal transition.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Diverse communities of bacterial endophytes inhabit plant tissues, and these bacteria play important roles for plant growth and health. Cherry laurel (Prunus laurocerasus L.) is a broadleaf evergreen shrub that is widely grown in temperate zones for its ornamental and medicinal properties, however virtually nothing is known about its associated bacterial community. In this study, we analysed the matured one-year-old leaves of this plant using Illumina-based 16S rRNA gene metabarcoding to reveal the community structure of endophytic bacteria and understand its shifts during the seasonal transition from winter dormancy to a spring vegetative state. The overall community was composed of four dominant phyla (Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes). Corynebacterium, Acinetobacter, and Chryseobacterium genera were the most prevalent bacteria, comprising 13.3%, 6.9%, and 6.8% of the amplicon sequence variants (ASVs), respectively. The ASV richness and diversity increased significantly in May as compared to other sampling months (February, March, and April). We observed high variation in the overall community structure of endophytic bacteria among collection dates. The variation was only reflected by a few core community members, suggesting that the changes of the endophytic community during winter/spring seasonal transition are mostly associated with the less abundant community members. We identified biomarker taxa for late winter, mid spring, and late spring collection dates. This study is the first one to report on the diversity and composition of bacterial endophytes in the leaves of cherry laurel and its shifts across the dormancy-to-vegetative seasonal transition. |
2021 |
Manz, C; Adamčík, S; Looney, B P; Corrales, A; Ovrebo, C; Adamčíková, K; Hofmann, T A; Hampe, F; Piepenbring, M Four new species of Russula subsection Roseinae from tropical montane forests in western Panama Journal Article PLoS ONE, 16 (10), pp. e0257616, 2021, ISSN: 1932-6203. @article{Manz2021, title = {Four new species of Russula subsection Roseinae from tropical montane forests in western Panama}, author = {C. Manz and S. Adamčík and B. P. Looney and A. Corrales and C. Ovrebo and K. Adamčíková and T. A. Hofmann and F. Hampe and M. Piepenbring }, url = {https://doi.org/10.1371/journal.pone.0257616}, doi = {doi.org/10.1371/journal.pone.0257616}, issn = {1932-6203}, year = {2021}, date = {2021-10-14}, journal = {PLoS ONE}, volume = {16}, number = {10}, pages = {e0257616}, abstract = {Species of the genus Russula are key components of ectomycorrhizal ecosystems worldwide. Nevertheless, their diversity in the tropics is still poorly known. This study aims to contribute to the knowledge of the diversity of Russula species classified in subsection Roseinae based on specimens recently collected in tropical montane rainforests in western Panama. A five gene multilocus phylogeny based on the nuclear markers ITS nrDNA, MCM7, RPB1, RPB2 and TEF-1α was constructed to identify the systematic position of 22 collections from Panama. Four new species, Russula cornicolor, Russula cynorhodon, Russula oreomunneae and Russula zephyrovelutipes are formally described and illustrated. None of the four species are sister species and they are more closely related to North American or Asian species. Two of the newly described species were associated with the ectomycorrhizal tree species Oreomunnea mexicana, while the other two species were associated with Quercus species. All four species are so far only known from mountains in western Panama.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species of the genus Russula are key components of ectomycorrhizal ecosystems worldwide. Nevertheless, their diversity in the tropics is still poorly known. This study aims to contribute to the knowledge of the diversity of Russula species classified in subsection Roseinae based on specimens recently collected in tropical montane rainforests in western Panama. A five gene multilocus phylogeny based on the nuclear markers ITS nrDNA, MCM7, RPB1, RPB2 and TEF-1α was constructed to identify the systematic position of 22 collections from Panama. Four new species, Russula cornicolor, Russula cynorhodon, Russula oreomunneae and Russula zephyrovelutipes are formally described and illustrated. None of the four species are sister species and they are more closely related to North American or Asian species. Two of the newly described species were associated with the ectomycorrhizal tree species Oreomunnea mexicana, while the other two species were associated with Quercus species. All four species are so far only known from mountains in western Panama. |
Adamson, K; Laas, M; Blumenstein, K; Busskamp, J; Langer, G J; Klavina, D; Kaur, A; Maaten, T; Mullett, M S; Müller, M M; Ondrušková, E; Padari, A; Pilt, E; Riit, T; Solheim, H; Soonvald, L; Tedersoo, L; Terhonen, E; Drenkhan, R Highly Clonal Structure and Abundance of One Haplotype Characterise the Diplodia sapinea Populations in Europe and Western Asia Journal Article Journal of Fungi, 7 (8), pp. 634, 2021, ISBN: EISSN 2309-608X. @article{K.2021, title = {Highly Clonal Structure and Abundance of One Haplotype Characterise the Diplodia sapinea Populations in Europe and Western Asia}, author = {K. Adamson and M. Laas and K. Blumenstein and J. Busskamp and G.J. Langer and D. Klavina and A. Kaur and T. Maaten and M.S. Mullett and M.M. Müller and E. Ondrušková and A. Padari and E. Pilt and T. Riit and H. Solheim and L. Soonvald and L. Tedersoo and E. Terhonen and R. Drenkhan}, editor = {David S. Perlin}, url = { https://www.mdpi.com/2309-608X/7/8/634/pdf }, doi = {https://doi.org/10.3390/jof7080634}, isbn = { EISSN 2309-608X}, year = {2021}, date = {2021-08-04}, journal = {Journal of Fungi}, volume = {7}, number = {8}, pages = {634}, abstract = {Diplodia sapinea is a cosmopolitan endophyte and opportunistic pathogen having occurred on several conifer species in Europe for at least 200 years. In Europe, disease outbreaks have increased on several Pinus spp. in the last few decades. In this study, the genetic structure of the European and western Asian D. sapinea population were investigated using 13 microsatellite markers. In total, 425 isolates from 15 countries were analysed. A high clonal fraction and low genetic distance between most subpopulations was found. One single haplotype dominates the European population, being represented by 45.3% of all isolates and found in nearly all investigated countries. Three genetically distinct subpopulations were found: Central/North European, Italian and Georgian. The recently detected subpopulations of D. sapinea in northern Europe (Estonia) share several haplotypes with the German subpopulation. The northern European subpopulations (Latvia, Estonia and Finland) show relatively high genetic diversity compared to those in central Europe suggesting either that the fungus has existed in the North in an asymptomatic/endophytic mode for a long time or that it has spread recently by multiple introductions. Considerable genetic diversity was found even among isolates of a single tree as 16 isolates from a single tree resulted in lower clonal fraction index than most subpopulations in Europe, which might reflect cryptic sexual proliferation. According to currently published allelic patterns, D. sapinea most likely originates from North America or from some unsampled population in Asia or central America. In order to enable the detection of endophytic or latent infections of planting stock by D. sapinea, new species-specific PCR primers (DiSapi-F and Diplo-R) were designed. During the search for Diplodia isolates across the world for species specific primer development, we identified D. africana in California, USA, and in the Canary Islands, which are the first records of this species in North America and in Spain. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Diplodia sapinea is a cosmopolitan endophyte and opportunistic pathogen having occurred on several conifer species in Europe for at least 200 years. In Europe, disease outbreaks have increased on several Pinus spp. in the last few decades. In this study, the genetic structure of the European and western Asian D. sapinea population were investigated using 13 microsatellite markers. In total, 425 isolates from 15 countries were analysed. A high clonal fraction and low genetic distance between most subpopulations was found. One single haplotype dominates the European population, being represented by 45.3% of all isolates and found in nearly all investigated countries. Three genetically distinct subpopulations were found: Central/North European, Italian and Georgian. The recently detected subpopulations of D. sapinea in northern Europe (Estonia) share several haplotypes with the German subpopulation. The northern European subpopulations (Latvia, Estonia and Finland) show relatively high genetic diversity compared to those in central Europe suggesting either that the fungus has existed in the North in an asymptomatic/endophytic mode for a long time or that it has spread recently by multiple introductions. Considerable genetic diversity was found even among isolates of a single tree as 16 isolates from a single tree resulted in lower clonal fraction index than most subpopulations in Europe, which might reflect cryptic sexual proliferation. According to currently published allelic patterns, D. sapinea most likely originates from North America or from some unsampled population in Asia or central America. In order to enable the detection of endophytic or latent infections of planting stock by D. sapinea, new species-specific PCR primers (DiSapi-F and Diplo-R) were designed. During the search for Diplodia isolates across the world for species specific primer development, we identified D. africana in California, USA, and in the Canary Islands, which are the first records of this species in North America and in Spain. |
Lange, De R; Adamčík, S; Adamčíková, K; Asselman, P; Borovička, J; Delgat, L; Hampe, F; Verbeken, A Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex Journal Article IMA Fungus, 20 (Article ID 12), pp. 1-31, 2021, ISSN: 2210-6359. @article{Lange2020, title = {Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex}, author = {R. De Lange and S. Adamčík and K. Adamčíková and P. Asselman and J. Borovička and L. Delgat and F. Hampe and A. Verbeken}, url = {https://imafungus.biomedcentral.com/articles/10.1186/s43008-021-00064-0}, doi = {10.1186/s43008-021-00064-0}, issn = {2210-6359}, year = {2021}, date = {2021-08-02}, journal = {IMA Fungus}, volume = {20}, number = {Article ID 12}, pages = {1-31}, abstract = {Russula albonigra is considered a well-known species, morphologically delimited by the context of the basidiomata blackening without intermediate reddening, and the menthol-cooling taste of the lamellae. It is supposed to have a broad ecological range and a large distribution area. A thorough molecular analysis based on four nuclear markers (ITS, LSU, RPB2 and TEF1-α) shows this traditional concept of R. albonigra s. lat. represents a species complex consisting of at least five European, three North American, and one Chinese species. Morphological study shows traditional characters used to delimit R. albonigra are not always reliable. Therefore, a new delimitation of the R. albonigra complex is proposed and a key to the described European species of R. subgen. Compactae is presented. A lectotype and an epitype are designated for R. albonigra and three new European species are described: R. ambusta, R. nigrifacta, and R. ustulata. Different thresholds of UNITE species hypotheses were tested against the taxonomic data. The distance threshold of 0.5% gives a perfect match to the phylogenetically defined species within the R. albonigra complex. Publicly available sequence data can contribute to species delimitation and increase our knowledge on ecology and distribution, but the pitfalls are short and low quality sequences.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Russula albonigra is considered a well-known species, morphologically delimited by the context of the basidiomata blackening without intermediate reddening, and the menthol-cooling taste of the lamellae. It is supposed to have a broad ecological range and a large distribution area. A thorough molecular analysis based on four nuclear markers (ITS, LSU, RPB2 and TEF1-α) shows this traditional concept of R. albonigra s. lat. represents a species complex consisting of at least five European, three North American, and one Chinese species. Morphological study shows traditional characters used to delimit R. albonigra are not always reliable. Therefore, a new delimitation of the R. albonigra complex is proposed and a key to the described European species of R. subgen. Compactae is presented. A lectotype and an epitype are designated for R. albonigra and three new European species are described: R. ambusta, R. nigrifacta, and R. ustulata. Different thresholds of UNITE species hypotheses were tested against the taxonomic data. The distance threshold of 0.5% gives a perfect match to the phylogenetically defined species within the R. albonigra complex. Publicly available sequence data can contribute to species delimitation and increase our knowledge on ecology and distribution, but the pitfalls are short and low quality sequences. |
Adamčíková, K; Jánošíková, Z; Adamčík, S; Ostrovský, R; Pastirčáková, K; Kobza, M; Ondrušková, E Host range, genetic variability, and mating types of Lecanosticta acicola in Slovakia Journal Article Scandinavian Journal of Forest Research, 36 (5), pp. 325-332, 2021, ISSN: 0282-7581. @article{Adamčíková2021b, title = {Host range, genetic variability, and mating types of \textit{Lecanosticta acicola} in Slovakia}, author = {K. Adamčíková and Z. Jánošíková and S. Adamčík and R. Ostrovský and K. Pastirčáková and M. Kobza and E. Ondrušková}, url = {https://doi.org/10.1080/02827581.2021.1941236}, doi = {10.1080/02827581.2021.1941236}, issn = {0282-7581}, year = {2021}, date = {2021-07-16}, journal = {Scandinavian Journal of Forest Research}, volume = {36}, number = {5}, pages = {325-332}, abstract = {In recent years, there has been an apparent Europe-wide emerging presence of brown spot needle blight, a disease of pine species caused by the fungus Lecanosticta acicola. In this study, we report the first well-documented occurrences of the pathogen in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region and TEF1 gene. Among the material collected from 84 locations within the country, L. acicola was present in 17 samples from 13 different locations, mainly distributed in urban environments. Four pine species were identified as hosts, among which, Pinus nigra and P. mugo were found to be the most frequently infected. Analysis of the mating type genes of 24 isolates obtained from two localities revealed the presence of a single mating type in Slovakia. All analyzed ITS sequences of the Slovak isolates were found to be uniform. However, although analysis of the TEF1 gene indicated that all Slovak isolates could be grouped into a single lineage, we detected nucleotide polymorphisms suggestive of a certain degree of genetic diversification within central European populations of the fungus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In recent years, there has been an apparent Europe-wide emerging presence of brown spot needle blight, a disease of pine species caused by the fungus Lecanosticta acicola. In this study, we report the first well-documented occurrences of the pathogen in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region and TEF1 gene. Among the material collected from 84 locations within the country, L. acicola was present in 17 samples from 13 different locations, mainly distributed in urban environments. Four pine species were identified as hosts, among which, Pinus nigra and P. mugo were found to be the most frequently infected. Analysis of the mating type genes of 24 isolates obtained from two localities revealed the presence of a single mating type in Slovakia. All analyzed ITS sequences of the Slovak isolates were found to be uniform. However, although analysis of the TEF1 gene indicated that all Slovak isolates could be grouped into a single lineage, we detected nucleotide polymorphisms suggestive of a certain degree of genetic diversification within central European populations of the fungus. |
Adamčíková, K; Jánošíková, Z; van der Nest, A; Adamčík, S; Ondrušková, E; Barnes, I Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia Journal Article Plant Pathology, 70 (8), pp. 1883-1896, 2021, ISSN: 1365-3059. @article{Adamčíková2021c, title = {Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia}, author = {K. Adamčíková and Z. Jánošíková and A. van der Nest and S. Adamčík and E. Ondrušková and I. Barnes }, url = {https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13428}, doi = {10.1111/ppa.13428}, issn = {1365-3059}, year = {2021}, date = {2021-06-30}, journal = {Plant Pathology}, volume = {70}, number = {8}, pages = {1883-1896}, abstract = {Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia. |
Hyblerová, Silvia; Medo, Juraj; Barta, Marek Annals of Forest Research, 64 (1), pp. 129-145, 2021, ISSN: 2065-2445. @article{Hyblerová2021, title = {Diversity and prevalence of entomopathogenic fungi (Ascomycota, Hypocreales) in epidemic populations of bark beetles (Coleoptera, Scolytinae) in spruce forests of the Tatra National Park in Slovakia}, author = {Silvia Hyblerová and Juraj Medo and Marek Barta}, url = {http://www.afrjournal.org/index.php/afr/article/view/2152}, doi = {10.15287/afr.2021.2152}, issn = {2065-2445}, year = {2021}, date = {2021-06-29}, journal = {Annals of Forest Research}, volume = {64}, number = {1}, pages = {129-145}, abstract = {Bark beetles are serious forest pests in Slovakia. Their outbreaks may have significant ecological and economic impacts on spruce forests. There is a variety of natural enemies that activate themselves during population outbreaks of insects and entomopathogenic fungi belong to important antagonists with a potential to regulate populations of their hosts. In 2014–2016, species richness and prevalence of entomopathogenic fungi were evaluated during the bark beetle outbreaks in spruce forests affected by windstorms in the Tatra National Park in Slovakia. Three Beauveria species, B. bassiana, B. caledonica and B. pseudobassiana, with Metapochonia bulbillosa were identified from 271 specimens of three bark beetle species, Ips typographus, Ips amitinus and Pityogenes chalcographus. Beauveria bassiana was the dominant pathogen and infected all three bark beetle species. Phylogenetic analysis identified three phylogenetic groups of B. bassiana in the evaluated host populations. M. bulbillosa was reported for the first time from bark beetle hosts and Slovakia. The prevalence of fungal infection in natural populations of I. typographus was low, varied between 0.07 and 0.72%, and have little influence on the bark beetle abundance. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Bark beetles are serious forest pests in Slovakia. Their outbreaks may have significant ecological and economic impacts on spruce forests. There is a variety of natural enemies that activate themselves during population outbreaks of insects and entomopathogenic fungi belong to important antagonists with a potential to regulate populations of their hosts. In 2014–2016, species richness and prevalence of entomopathogenic fungi were evaluated during the bark beetle outbreaks in spruce forests affected by windstorms in the Tatra National Park in Slovakia. Three Beauveria species, B. bassiana, B. caledonica and B. pseudobassiana, with Metapochonia bulbillosa were identified from 271 specimens of three bark beetle species, Ips typographus, Ips amitinus and Pityogenes chalcographus. Beauveria bassiana was the dominant pathogen and infected all three bark beetle species. Phylogenetic analysis identified three phylogenetic groups of B. bassiana in the evaluated host populations. M. bulbillosa was reported for the first time from bark beetle hosts and Slovakia. The prevalence of fungal infection in natural populations of I. typographus was low, varied between 0.07 and 0.72%, and have little influence on the bark beetle abundance. |
Jánošíková, Z; Dutech, C; Ondrušková, E; Adamčíková, K; Mullett, M Population structure and genetic diversity of Dothistroma septosporum in Slovakia Journal Article European Journal of Plant Pathology, 160 (4), pp. 771-787, 2021, ISSN: 1573-8469. @article{Jánošíková2021, title = {Population structure and genetic diversity of Dothistroma septosporum in Slovakia}, author = {Z. Jánošíková and C. Dutech and E. Ondrušková and K. Adamčíková and M. Mullett}, url = {https://link.springer.com/article/10.1007/s10658-021-02266-z}, doi = {doi:10.1007/s10658-021-02266-z}, issn = {1573-8469}, year = {2021}, date = {2021-06-23}, journal = {European Journal of Plant Pathology}, volume = {160}, number = {4}, pages = {771-787}, abstract = {Dothistroma needle blight (DNB) is a serious pine disease present worldwide caused by the ascomycetous fungi Dothistroma septosporum and Dothistroma pini. Based on multiplex PCR analysis of 11 microsatellite markers screened on 253 D. septosporum isolates obtained from 32 sites across Slovakia, a total of 137 unique multilocus haplotypes (MLHs) were detected. The majority of MLHs (n = 91) were represented by a single isolate, but 13 MLHs were found at two different sites, and in some cases, these were separated by large distances. Four population clusters were identified using a discriminant analysis of principal components (DAPC). The genetic clusters obtained from the DAPC were mixed throughout the country and were not restricted to particular host species. Although both mating types of D. septosporum were found to be in an almost perfect 1:1 ratio in both the non-clone-corrected and clone-corrected datasets, random mating was rejected in the entire dataset. Random mating was only supported in the Pinus mugo group planted in urban areas and on a smaller spatial scale in two sites (Kálnica and Litava) using the clonecorrected dataset. These findings suggest a mixed mode of reproduction with an important component of sexual reproduction, although the sexual stage of the fungus (teleomorph) has not been physically observed in Slovakia yet. The examination of spatial relationships using spatial principal component analyses and the presence of isolation-by-distance together with relatively high genetic diversity suggests the pathogen has been long established in Slovakia and spread naturally across the landscape. However, the weak population structure and findings of identical clones at widely separated sites strongly suggests some degree of human assisted dispersal.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a serious pine disease present worldwide caused by the ascomycetous fungi Dothistroma septosporum and Dothistroma pini. Based on multiplex PCR analysis of 11 microsatellite markers screened on 253 D. septosporum isolates obtained from 32 sites across Slovakia, a total of 137 unique multilocus haplotypes (MLHs) were detected. The majority of MLHs (n = 91) were represented by a single isolate, but 13 MLHs were found at two different sites, and in some cases, these were separated by large distances. Four population clusters were identified using a discriminant analysis of principal components (DAPC). The genetic clusters obtained from the DAPC were mixed throughout the country and were not restricted to particular host species. Although both mating types of D. septosporum were found to be in an almost perfect 1:1 ratio in both the non-clone-corrected and clone-corrected datasets, random mating was rejected in the entire dataset. Random mating was only supported in the Pinus mugo group planted in urban areas and on a smaller spatial scale in two sites (Kálnica and Litava) using the clonecorrected dataset. These findings suggest a mixed mode of reproduction with an important component of sexual reproduction, although the sexual stage of the fungus (teleomorph) has not been physically observed in Slovakia yet. The examination of spatial relationships using spatial principal component analyses and the presence of isolation-by-distance together with relatively high genetic diversity suggests the pathogen has been long established in Slovakia and spread naturally across the landscape. However, the weak population structure and findings of identical clones at widely separated sites strongly suggests some degree of human assisted dispersal. |
Horáková, Miriam Kádasi; Tancik, Jan; Barta, Marek Fusarium proliferatum causing dry rot of stored garlic in Slovakia Journal Article Journal of Plant Pathology, 2021, ISSN: 2239-7264. @article{Horáková2021, title = {Fusarium proliferatum causing dry rot of stored garlic in Slovakia}, author = {Miriam Kádasi Horáková and Jan Tancik and Marek Barta}, doi = {10.1007/s42161-021-00883-5}, issn = {2239-7264}, year = {2021}, date = {2021-06-10}, journal = {Journal of Plant Pathology}, abstract = {Symptoms of dry clove rot of Allium sativum produced in Slovakia were noticed during storage in 2019. In 2020, garlic bulbs of eight cultivars were analysed and fungal isolates were obtained. Morphological and molecular identification confirmed the isolation of Fusarium proliferatum from all cultivars. The mean proportion of symptomatic cloves in evaluated samples reached 78.68%, and the mean area of clove surface damaged by the disease ranged from 5.97% to 14.83% 5 weeks after the harvest. The pathogenicity of the selected F. proliferatum isolate was verified by inoculation of garlic cloves in laboratory bioassays. This is the first report of F. proliferatum causing dry rot of garlic bulbs in Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Symptoms of dry clove rot of Allium sativum produced in Slovakia were noticed during storage in 2019. In 2020, garlic bulbs of eight cultivars were analysed and fungal isolates were obtained. Morphological and molecular identification confirmed the isolation of Fusarium proliferatum from all cultivars. The mean proportion of symptomatic cloves in evaluated samples reached 78.68%, and the mean area of clove surface damaged by the disease ranged from 5.97% to 14.83% 5 weeks after the harvest. The pathogenicity of the selected F. proliferatum isolate was verified by inoculation of garlic cloves in laboratory bioassays. This is the first report of F. proliferatum causing dry rot of garlic bulbs in Slovakia. |
Vera, M; Adamčík, S; Adamčíková, K; Hampe, F; Caboň, M; Manz, C; Overbo, C; Peipenbring, M; Corrales, A Morphological and genetic diversification of Russula floriformis, sp. nov., along the Isthmus of Panama Journal Article Mycologia, 113 (4), pp. 807-827, 2021, ISSN: 1557-2536. @article{Vera2021, title = {Morphological and genetic diversification of Russula floriformis, sp. nov., along the Isthmus of Panama}, author = {M. Vera and S. Adamčík and K. Adamčíková and F. Hampe and M. Caboň and C. Manz and C. Overbo and M. Peipenbring and A. Corrales}, url = {https://www.tandfonline.com/doi/full/10.1080/00275514.2021.1897377}, doi = {DOI: 10.1080/00275514.2021.1897377}, issn = {1557-2536}, year = {2021}, date = {2021-05-27}, journal = {Mycologia}, volume = {113}, number = {4}, pages = {807-827}, abstract = {Species of Russula are ubiquitous members of ectomycorrhizal fungal communities in tropical ecosystems. However, an important part of the total tropical diversity of this genus and its biogeographic patterns is unknown due to the lack of studies on Russula in tropical ecosystems. We combined molecular, morphological, ecological, and biogeographic data to elaborate concepts for two new subspecies of R. floriformis (subsection Substriatinae). Russula floriformis subsp. floriformis and R. floriformis subsp. symphoniae are described as new from montane forest dominated by Quercus and/or Oreomunnea (Fagales) from Colombia and Panama, respectively. Phylogenies were constructed using nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), D1–D2 domains of nuc 28S rDNA (28S), and partial regions of the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-alpha (tef1). Similar environmental conditions, similar morphology, and an ITS sequence similarity higher than 99% with only three different positions indicate that these two subspecies are closely related. Detailed observations of microscopic structures and analyses of further DNA loci, however, revealed morphological and molecular characteristics that allow distinguishing the two subspecies of R. floriformis. Spatial distribution and phylogenetic proximity of the two Russula subspecies and their ectomycorrhizal hosts, i.e., species of Quercus, suggest that their diversification is a result of comigration, adaptation, and geographic isolation along the Isthmus of Panama during the Pliocene and Pleistocene.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species of Russula are ubiquitous members of ectomycorrhizal fungal communities in tropical ecosystems. However, an important part of the total tropical diversity of this genus and its biogeographic patterns is unknown due to the lack of studies on Russula in tropical ecosystems. We combined molecular, morphological, ecological, and biogeographic data to elaborate concepts for two new subspecies of R. floriformis (subsection Substriatinae). Russula floriformis subsp. floriformis and R. floriformis subsp. symphoniae are described as new from montane forest dominated by Quercus and/or Oreomunnea (Fagales) from Colombia and Panama, respectively. Phylogenies were constructed using nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), D1–D2 domains of nuc 28S rDNA (28S), and partial regions of the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-alpha (tef1). Similar environmental conditions, similar morphology, and an ITS sequence similarity higher than 99% with only three different positions indicate that these two subspecies are closely related. Detailed observations of microscopic structures and analyses of further DNA loci, however, revealed morphological and molecular characteristics that allow distinguishing the two subspecies of R. floriformis. Spatial distribution and phylogenetic proximity of the two Russula subspecies and their ectomycorrhizal hosts, i.e., species of Quercus, suggest that their diversification is a result of comigration, adaptation, and geographic isolation along the Isthmus of Panama during the Pliocene and Pleistocene. |
Adamčíková, K; Ondrušková, E; Pažitný, J; Jánošíková, Z Divergence in culture morphology between two related species, Dothistroma septosporum and D. pini Journal Article Czech Mycology, 73 (1), pp. 109-119, 2021, ISSN: 1805-1421. @article{Adamčíková2021, title = {Divergence in culture morphology between two related species, Dothistroma septosporum and D. pini}, author = {K. Adamčíková and E. Ondrušková and J. Pažitný and Z. Jánošíková}, url = {http://ife.sk/wp-content/uploads/2020/09/fig.-2-rev-5.tif}, doi = {doi.org/10.33585/cmy.73108}, issn = {1805-1421}, year = {2021}, date = {2021-05-24}, journal = {Czech Mycology}, volume = {73}, number = {1}, pages = {109-119}, abstract = {Dothistroma needle blight (DNB) is one of the most important pine needle diseases worldwide. The disease is caused by two fungal species, Dothistroma septosporum and D. pini, which are similar not only in terms of their ecology, causing similar symptoms in hosts of the genus Pinus, but also in terms of their morphological characteristics. The morphologies of 353 cultured Dothistroma isolates from 11 different pine species in Slovakia and their relation to Dothistroma species and/or fungus origin (host) were studied and evaluated by means of the Chi-square test. The cultures were classified into eight categories according to pigmentation. Dothistroma pini isolates were assigned to 4 of the 8 categories, and D. septosporum isolates were assigned to all 8 categories with varying frequencies. The Chi-square test revealed that the culture morphology categories of D. pini and D. septosporum differed significantly. Interactions between the evaluated factors (culture morphology, Dothistroma and host species) were analysed and showed significant differences.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is one of the most important pine needle diseases worldwide. The disease is caused by two fungal species, Dothistroma septosporum and D. pini, which are similar not only in terms of their ecology, causing similar symptoms in hosts of the genus Pinus, but also in terms of their morphological characteristics. The morphologies of 353 cultured Dothistroma isolates from 11 different pine species in Slovakia and their relation to Dothistroma species and/or fungus origin (host) were studied and evaluated by means of the Chi-square test. The cultures were classified into eight categories according to pigmentation. Dothistroma pini isolates were assigned to 4 of the 8 categories, and D. septosporum isolates were assigned to all 8 categories with varying frequencies. The Chi-square test revealed that the culture morphology categories of D. pini and D. septosporum differed significantly. Interactions between the evaluated factors (culture morphology, Dothistroma and host species) were analysed and showed significant differences. |
Piršelová, B; Ondrušková, E Effect of Cadmium Chloride and Cadmium Nitrate on Growth and Mineral Nutrient Content in the Root of Fava Bean (Vicia faba L.) Journal Article Plants, 10 (5), pp. 1007, 2021. @article{B.2021, title = {Effect of Cadmium Chloride and Cadmium Nitrate on Growth and Mineral Nutrient Content in the Root of Fava Bean (Vicia faba L.)}, author = {B. Piršelová and E. Ondrušková}, editor = {Barceló J.}, url = {https://www.mdpi.com/2223-7747/10/5/1007}, doi = { https://doi.org/10.3390/plants10051007}, year = {2021}, date = {2021-05-18}, journal = {Plants}, volume = {10}, number = {5}, pages = {1007}, abstract = {The present study aimed to analyze the differences in the tolerance of fava bean (Vicia faba cv. Aštar) roots to cadmium in nitrate—Cd(NO3)2—and chloride—CdCl2—solutions. The physiological and biochemical parameters were assessed. The tested doses of Cd (50, 100, 150 and 300 mg/L) did not influence the germination of seeds. However, considerable growth inhibition and dehydration were observed after 96 h incubation. The thickness of roots and rupture of cell membranes increased along with the increasing concentration of the metal in the solution. At a Cd dose of 300 mg/L, irrespective of the solution used, increased nitrogen concentration and no change in sodium content were observed. The content of magnesium increased due to the dose of 100 mg/L (cadmium nitrate) and the content of calcium increased due to the dose of 300 mg/L (in either nitrate or chloride). The correlation analyses pointed to a possible effect of nitrates in the applied solutions on the accumulation of Cd and some minerals in the roots of the given variety of fava bean. This may be important for both research and agricultural practice. The identification of crops with high tolerance to cadmium, as well as knowledge about the mechanisms of ion interactions at the soil solution–plant level, is important in terms of such crops’ use in the process of the remediation of cadmium-contaminated soils coupled with food production.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The present study aimed to analyze the differences in the tolerance of fava bean (Vicia faba cv. Aštar) roots to cadmium in nitrate—Cd(NO3)2—and chloride—CdCl2—solutions. The physiological and biochemical parameters were assessed. The tested doses of Cd (50, 100, 150 and 300 mg/L) did not influence the germination of seeds. However, considerable growth inhibition and dehydration were observed after 96 h incubation. The thickness of roots and rupture of cell membranes increased along with the increasing concentration of the metal in the solution. At a Cd dose of 300 mg/L, irrespective of the solution used, increased nitrogen concentration and no change in sodium content were observed. The content of magnesium increased due to the dose of 100 mg/L (cadmium nitrate) and the content of calcium increased due to the dose of 300 mg/L (in either nitrate or chloride). The correlation analyses pointed to a possible effect of nitrates in the applied solutions on the accumulation of Cd and some minerals in the roots of the given variety of fava bean. This may be important for both research and agricultural practice. The identification of crops with high tolerance to cadmium, as well as knowledge about the mechanisms of ion interactions at the soil solution–plant level, is important in terms of such crops’ use in the process of the remediation of cadmium-contaminated soils coupled with food production. |
Pastirčáková, K; Adamčík, S; Adamčíková, K; Chater, A O Erysiphe hypophylla, a second powdery mildew (Erysiphales) on oaks in Britain Journal Article Field Mycology, 22 (2), pp. 50-54, 2021, ISSN: 1468-1641. @article{Pastirčáková2021b, title = {\textit{Erysiphe hypophylla}, a second powdery mildew (\textit{Erysiphales}) on oaks in Britain}, author = {K. Pastirčáková and S. Adamčík and K. Adamčíková and A.O. Chater}, doi = {10.1016/j.fldmyc.2021.04.008}, issn = {1468-1641}, year = {2021}, date = {2021-04-20}, journal = {Field Mycology}, volume = {22}, number = {2}, pages = {50-54}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Takov, Danail; Pilarska, Daniela; Linde, Andreas; Barta, Marek Infectious and parasitic diseases of phytophagous insect pests in the context of extreme environmental conditions Journal Article Central European Forestry Journal, 67 (2), pp. 72-84, 2021, ISSN: 2454-0358. @article{Takov2021, title = {Infectious and parasitic diseases of phytophagous insect pests in the context of extreme environmental conditions}, author = {Danail Takov and Daniela Pilarska and Andreas Linde and Marek Barta}, url = {https://web.nlcsk.org/wp-content/uploads/2021/04/Takov.pdf}, doi = {10.2478/forj-2020-0018}, issn = {2454-0358}, year = {2021}, date = {2021-04-01}, journal = {Central European Forestry Journal}, volume = {67}, number = {2}, pages = {72-84}, abstract = {The density of phytophagous insect pest populations is related (directly and indirectly) to several groups of factors that can be broadly divided into: abiotic, biotic and anthropogenic. Each extreme in the abiotic environment at a macro-level leads to a series of consecutive extremes in the biotic environment, which eventually results in micro-level responses in the individual organisms. The manifestation of factors acts in aggregate or in a sequence, creating a chain of processes around us. Insects very efficiently use the abundance of nutritional resources, resulting in a tremendous increase in their population density, and triggering control mechanisms through the emergence of parasitic and pathogenic infections (viruses, bacteria, fungi, microsporidia, protozoa and nematodes). The development of entomopathogenic infections in host populations is directly dependent on the characteristics of both the antagonist and the insect. It is associated with the lifestyle and life cycle of the insect, with features encoded in the mechanism of pathogen action, and limited by the pathogen’s virulence and pathogenicity.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The density of phytophagous insect pest populations is related (directly and indirectly) to several groups of factors that can be broadly divided into: abiotic, biotic and anthropogenic. Each extreme in the abiotic environment at a macro-level leads to a series of consecutive extremes in the biotic environment, which eventually results in micro-level responses in the individual organisms. The manifestation of factors acts in aggregate or in a sequence, creating a chain of processes around us. Insects very efficiently use the abundance of nutritional resources, resulting in a tremendous increase in their population density, and triggering control mechanisms through the emergence of parasitic and pathogenic infections (viruses, bacteria, fungi, microsporidia, protozoa and nematodes). The development of entomopathogenic infections in host populations is directly dependent on the characteristics of both the antagonist and the insect. It is associated with the lifestyle and life cycle of the insect, with features encoded in the mechanism of pathogen action, and limited by the pathogen’s virulence and pathogenicity. |
Zúbrik, Milan; Kunca, Andrej; Kulfan, Ján; Rell, Slavomír; Nikolov, Christo; Galko, Juraj; Vakula, Jozef; Gubka, Andrej; Leontovyč, Roman; Konôpka, Bohdan; Lalík, Michal; Longauerová, Valéria; Sitková, Zuzana; Liška, Jan; Zach, Peter; Barta, Marek; Holuša, Jaroslav Occurrence of gypsy moth (Lymantria dispar L.) in the Slovak Republic and its outbreaks during 1945–2020 Journal Article Central European Forestry Journal, 67 (2), pp. 55-71, 2021, ISSN: 2454-0358. @article{Zúbrik2021, title = {Occurrence of gypsy moth (Lymantria dispar L.) in the Slovak Republic and its outbreaks during 1945–2020}, author = {Milan Zúbrik and Andrej Kunca and Ján Kulfan and Slavomír Rell and Christo Nikolov and Juraj Galko and Jozef Vakula and Andrej Gubka and Roman Leontovyč and Bohdan Konôpka and Michal Lalík and Valéria Longauerová and Zuzana Sitková and Jan Liška and Peter Zach and Marek Barta and Jaroslav Holuša}, url = {https://web.nlcsk.org/wp-content/uploads/2021/04/Zubrik_etal.pdf}, doi = {10.2478/forj-2021-0007}, issn = {2454-0358}, year = {2021}, date = {2021-04-01}, journal = {Central European Forestry Journal}, volume = {67}, number = {2}, pages = {55-71}, abstract = {The gypsy moth is one of the most serious pests in forests and fruit tree plantations over prevailing parts of the Northern Hemisphere. This work is based on a literature review, and presents history of gypsy moth Lymantria dispar L., observed in Slovak forests within the period 1945–2020. The life cycle, hosts, natural enemies, population dynamics of pests, impact of outbreaks on forests and different management methods used in the past are discussed. Since 1945, there were nine gypsy moth outbreaks in Slovakia. Between 1945 and 2020, a total of 155,034 ha of deciduous forests were touched with varying intensity, representing an average annual damage of 2,040 ha. The strongest outbreak culminated in 2004. Totally 51,479 ha were attacked in the period of 2000–2008. We have found outbreak periods that repeat with frequency of 7.8 ±2.2 years and the average outbreak phase lasts 3.1 ±1.1 years. The period between two subsequent outbreaks seems to be more or less constant and duration of the outbreak phase seems to be gradually shortened during the study period. Several factors influencing the gypsy moth population dynamics in Slovakia are discussed. The role of biological control by using entomopathogenic fungus Entomophaga maimaiga is described.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The gypsy moth is one of the most serious pests in forests and fruit tree plantations over prevailing parts of the Northern Hemisphere. This work is based on a literature review, and presents history of gypsy moth Lymantria dispar L., observed in Slovak forests within the period 1945–2020. The life cycle, hosts, natural enemies, population dynamics of pests, impact of outbreaks on forests and different management methods used in the past are discussed. Since 1945, there were nine gypsy moth outbreaks in Slovakia. Between 1945 and 2020, a total of 155,034 ha of deciduous forests were touched with varying intensity, representing an average annual damage of 2,040 ha. The strongest outbreak culminated in 2004. Totally 51,479 ha were attacked in the period of 2000–2008. We have found outbreak periods that repeat with frequency of 7.8 ±2.2 years and the average outbreak phase lasts 3.1 ±1.1 years. The period between two subsequent outbreaks seems to be more or less constant and duration of the outbreak phase seems to be gradually shortened during the study period. Several factors influencing the gypsy moth population dynamics in Slovakia are discussed. The role of biological control by using entomopathogenic fungus Entomophaga maimaiga is described. |
Lalík, Michal; Galko, Juraj; Nikolov, Christo; Rell, Slavomír; Kunca, Andrej; Zúbrik, Milan; Hyblerová, Silvia; Barta, Marek; Holuša, Jaroslav Potential of Beauveria bassiana application via a carrier to control the large pine weevil Journal Article Crop Protection, 143 , pp. 105563, 2021, ISSN: 0261-2194. @article{Lalík2021, title = {Potential of Beauveria bassiana application via a carrier to control the large pine weevil}, author = {Michal Lalík and Juraj Galko and Christo Nikolov and Slavomír Rell and Andrej Kunca and Milan Zúbrik and Silvia Hyblerová and Marek Barta and Jaroslav Holuša}, doi = {https://doi.org/10.1016/j.cropro.2021.105563}, issn = {0261-2194}, year = {2021}, date = {2021-03-02}, journal = {Crop Protection}, volume = {143}, pages = {105563}, abstract = {The large pine weevil (Hylobius abietis) is an important pest of freshly planted coniferous trees. Feeding damage to conifer seedlings can be prevented by protecting the seedlings (mechanically or chemically), or by reducing H. abietis abundance. The entomopathogenic fungus Beauveria bassiana has promise for H. abietis control. To test the effects of the B. bassiana-colonized carriers (25 mm in diameter and colonized by strain AMEP20) on H. abietis adults that move into forest clearances, we performed several experiments. These included an experiment in Petri dishes under laboratory conditions, an experiment in cages with Norway spruce seedlings under semi-natural conditions, and a field experiment. Infection by B. bassiana and mortality of H. abietis was confirmed in all three experiments. The total mortality rate in all Petri dishes with B. bassiana carriers after 14 days was 74% (80% with fresh carriers; 68% with carriers that had been treated with UV light; 82% for carriers that had been frozen; 45% for adults that were exposed to carriers for only 24 h; and 95% for carriers that had been stored at 4 °C for 2 months). The mortality of H. abietis adults was affected by the absence/presence of carriers in the cages with spruce plants. The number of dead adults after 4 weeks of treatment in forest clearing were higher in plots with carriers than in the control plot. Mortality was highest for adults trapped in the plot with carriers on trap barks (58%), followed by the plot with carriers and an attractant (48%), and followed by the plots with carriers without an attractant (35%). The results suggest that carriers colonized by the entomopathogenic fungus B. bassiana could be suitable for reducing H. abietis abundance in forests. The results indicated that H. abietis adults may be infected by B. bassiana after only a short exposure to carriers. The B. bassiana-colonized carrier is ready-to-use, i.e., it could be immediately produced and applied.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The large pine weevil (Hylobius abietis) is an important pest of freshly planted coniferous trees. Feeding damage to conifer seedlings can be prevented by protecting the seedlings (mechanically or chemically), or by reducing H. abietis abundance. The entomopathogenic fungus Beauveria bassiana has promise for H. abietis control. To test the effects of the B. bassiana-colonized carriers (25 mm in diameter and colonized by strain AMEP20) on H. abietis adults that move into forest clearances, we performed several experiments. These included an experiment in Petri dishes under laboratory conditions, an experiment in cages with Norway spruce seedlings under semi-natural conditions, and a field experiment. Infection by B. bassiana and mortality of H. abietis was confirmed in all three experiments. The total mortality rate in all Petri dishes with B. bassiana carriers after 14 days was 74% (80% with fresh carriers; 68% with carriers that had been treated with UV light; 82% for carriers that had been frozen; 45% for adults that were exposed to carriers for only 24 h; and 95% for carriers that had been stored at 4 °C for 2 months). The mortality of H. abietis adults was affected by the absence/presence of carriers in the cages with spruce plants. The number of dead adults after 4 weeks of treatment in forest clearing were higher in plots with carriers than in the control plot. Mortality was highest for adults trapped in the plot with carriers on trap barks (58%), followed by the plot with carriers and an attractant (48%), and followed by the plots with carriers without an attractant (35%). The results suggest that carriers colonized by the entomopathogenic fungus B. bassiana could be suitable for reducing H. abietis abundance in forests. The results indicated that H. abietis adults may be infected by B. bassiana after only a short exposure to carriers. The B. bassiana-colonized carrier is ready-to-use, i.e., it could be immediately produced and applied. |
Mullett, M S; Drenkhan, R; Adamson, K; Boroń, P; Lenart-Boroń, A; Barnes, I; Tomšovský, M; Jánošíková, Z; Adamčíková, K; Ondrušková, E; Queloz, V; Piškur, B; Musolin, D L; Davydenko, K; Georgieva, M; Schmitz, S; Kačergius, A; Ghelardini, L; Orlović, Kranjec J; Müller, M; Oskay, F; Hauptman, T; Halász, Á; Markovskaja, S; Solheim, H; Vuorinen, M; Heinzelmann, R; Hamelin, R C; Konečný, A Journal of Fungi, 7 (2), pp. Article number: 111, 2021, ISBN: 2309-608X. @article{Mullett2021, title = {Worldwide Genetic Structure Elucidates the Eurasian Origin and Invasion Pathways of Dothistroma septosporum, Causal Agent of Dothistroma Needle Blight }, author = {M. S. Mullett and R. Drenkhan and K. Adamson and P. Boroń and A. Lenart-Boroń and I. Barnes and M. Tomšovský and Z. Jánošíková and K. Adamčíková and E. Ondrušková and V. Queloz and B. Piškur and D.L. Musolin and K. Davydenko and M. Georgieva and S. Schmitz and A. Kačergius and L. Ghelardini and J. Kranjec Orlović and M. Müller and F. Oskay and T. Hauptman and Á. Halász and S. Markovskaja and H. Solheim and M. Vuorinen and R. Heinzelmann and R.C. Hamelin and A. Konečný}, doi = {doi 10.3390/jof7020111 }, isbn = {2309-608X}, year = {2021}, date = {2021-02-03}, journal = {Journal of Fungi}, volume = {7}, number = {2}, pages = {Article number: 111}, abstract = {Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia. |
Sánchez-García, M; Adamčíková, K; Moreau, P -A; Vizzini, A; Jančovičová, S; Kiran, M; Caboň, M; Matheny, P B; Adamčík, S The genus Dermoloma is more diverse than expected and forms a monophyletic lineage in the Tricholomataceae Journal Article Mycological Progress, 20 (1), pp. 11–25, 2021, ISSN: 1617-416X. @article{Sánchez-García2021, title = {The genus Dermoloma is more diverse than expected and forms a monophyletic lineage in the Tricholomataceae}, author = {M. Sánchez-García and K. Adamčíková and P.-A. Moreau and A. Vizzini and S. Jančovičová and M. Kiran and M. Caboň and P. B. Matheny and S. Adamčík}, doi = {doi 10.1007/s11557-020-01651-y}, issn = {1617-416X}, year = {2021}, date = {2021-01-18}, journal = {Mycological Progress}, volume = {20}, number = {1}, pages = {11–25}, abstract = {We present the first phylogenetic evaluation of the genus Dermoloma, which is resolved as monophyletic and closely related to Pseudotricholoma, a poorly known Dermoloma-like lineage within the family Tricholomataceae. The position of Dermoloma is confirmed by the placement of the type species, Dermoloma cuneifolium, represented by multiple samples including the neotype. Based on our phylogenetic analyses, we recognised 25 European operational taxonomic units (OTUs) but could only assign species names to ten of them based on ex-type sequences. Furthermore, only five additional published Dermoloma names of uncertain status are available for the remaining 16 potential European species, thus demonstrating an unexpected amount of taxonomic diversity. Samples from Europe and North America seem to be endemic on a continental scale. North American samples formed six unique OTUs, but only one could be reliably named, Dermoloma hymenocephalum. Dermoloma is morphologically defined by basidiomata with brown, grey and white colours with a farinaceous odour and a pluristratous hymeniderm type of pileipellis. Our phylogenetic analyses support the subdivision of the genus into two subgenera and four sections, and species with inamyloid basidiospores are placed in subg. Dermoloma and those with amyloid basidiospores in subg. Amylospora. Both subgenera are further divided into two sections. The analysis of spore morphology shows that sect. Conica of subg. Dermoloma and sect. Nigrescentia of subg. Amylospora have a very distinctive spore shape. Sect. Atrobrunnea of subg. Amylospora showed relatively high variability of spores among species, but spores of sect. Dermoloma were similar and not useful for species discrimination.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We present the first phylogenetic evaluation of the genus Dermoloma, which is resolved as monophyletic and closely related to Pseudotricholoma, a poorly known Dermoloma-like lineage within the family Tricholomataceae. The position of Dermoloma is confirmed by the placement of the type species, Dermoloma cuneifolium, represented by multiple samples including the neotype. Based on our phylogenetic analyses, we recognised 25 European operational taxonomic units (OTUs) but could only assign species names to ten of them based on ex-type sequences. Furthermore, only five additional published Dermoloma names of uncertain status are available for the remaining 16 potential European species, thus demonstrating an unexpected amount of taxonomic diversity. Samples from Europe and North America seem to be endemic on a continental scale. North American samples formed six unique OTUs, but only one could be reliably named, Dermoloma hymenocephalum. Dermoloma is morphologically defined by basidiomata with brown, grey and white colours with a farinaceous odour and a pluristratous hymeniderm type of pileipellis. Our phylogenetic analyses support the subdivision of the genus into two subgenera and four sections, and species with inamyloid basidiospores are placed in subg. Dermoloma and those with amyloid basidiospores in subg. Amylospora. Both subgenera are further divided into two sections. The analysis of spore morphology shows that sect. Conica of subg. Dermoloma and sect. Nigrescentia of subg. Amylospora have a very distinctive spore shape. Sect. Atrobrunnea of subg. Amylospora showed relatively high variability of spores among species, but spores of sect. Dermoloma were similar and not useful for species discrimination. |
Pastirčáková, Katarína; Adamčíková, Katarína; Bacigálová, Kamila; Caboň, Miroslav; Mikušová, Petra; Senko, Dušan; Svitok, Marek; Adamčík, Slavomír Ash Trees (Fraxinus spp.) in Urban Greenery as Possible Invasion Gates of Non-Native Phyllactinia Species Journal Article Forests, 12 (2), pp. 183, 2021, ISSN: 1999-4907. @article{Pastir_kov__2021, title = {Ash Trees (Fraxinus spp.) in Urban Greenery as Possible Invasion Gates of Non-Native Phyllactinia Species}, author = {Katarína Pastirčáková and Katarína Adamčíková and Kamila Bacigálová and Miroslav Caboň and Petra Mikušová and Dušan Senko and Marek Svitok and Slavomír Adamčík}, url = {http://dx.doi.org/10.3390/f12020183}, doi = {10.3390/f12020183}, issn = {1999-4907}, year = {2021}, date = {2021-01-01}, journal = {Forests}, volume = {12}, number = {2}, pages = {183}, publisher = {MDPI AG}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2020 |
Bičárová, S; Shashikumar, A; Richier, Dalstein- L; Lukasová, V; Adamčíková, K; Pavlendová, H; Sitková, Z; Buchholcerová, A; Bilčík, D The response of Pinus species to ozone uptake in different climate regions of Europe Journal Article Central European Forestry Journal , 66 (4), pp. 255–268, 2020, ISBN: 2454-034X. @article{Bičárová2020, title = {The response of Pinus species to ozone uptake in different climate regions of Europe}, author = {S. Bičárová and A. Shashikumar and L. Dalstein- Richier and V. Lukasová and K. Adamčíková and H. Pavlendová and Z. Sitková and A. Buchholcerová and D. Bilčík}, doi = {DOI: 10.2478/forj-2020-0022}, isbn = {2454-034X}, year = {2020}, date = {2020-12-04}, journal = {Central European Forestry Journal }, volume = {66}, number = {4}, pages = {255–268}, abstract = {This study is focused on the research of selected Pinus species exposed to high ozone concentrations in the mountain environment. We noticed different values of modelled ozone doses (MOD) up-taken by Mountain pine (Pinus mugo Turra) in the High Tatra Mts (SK–HTMts) and Swiss stone pine (Pinus cembra L.) in the Alpes-Mercantour (FR–AlpMar) during the growing season 2019. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. Testing has shown that ozone input based on passive sampling may be used in MOD modelling for sites situated in the subalpine zone where the operation of active monitors is limited. . Presented results confirmed the assumption regarding stomatal ozone flux reduction due to the occurrence of soil drought in hot and dry summer weather typical for the Mediterranean climate region. Despite the limitation of stomatal flux, foliar ozone specific injury on two years needles of P. cembra was substantially higher in comparison to the incidence of ozone injury symptoms observed on two years needles of P. mugo in SK–HTMts. It may suggest low phytotoxicity of given MOD or efficient resistance of P. mugo against oxidative stress. In addition, the visible injury index (VINX) covering the broad effect of biotic and abiotic harmful agents was appraised on P. mugo. Percentage of affected surface indicated moderate deterioration of needle injury at the end of the growing season, particularly due to traces of mechanical damage.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study is focused on the research of selected Pinus species exposed to high ozone concentrations in the mountain environment. We noticed different values of modelled ozone doses (MOD) up-taken by Mountain pine (Pinus mugo Turra) in the High Tatra Mts (SK–HTMts) and Swiss stone pine (Pinus cembra L.) in the Alpes-Mercantour (FR–AlpMar) during the growing season 2019. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. Testing has shown that ozone input based on passive sampling may be used in MOD modelling for sites situated in the subalpine zone where the operation of active monitors is limited. . Presented results confirmed the assumption regarding stomatal ozone flux reduction due to the occurrence of soil drought in hot and dry summer weather typical for the Mediterranean climate region. Despite the limitation of stomatal flux, foliar ozone specific injury on two years needles of P. cembra was substantially higher in comparison to the incidence of ozone injury symptoms observed on two years needles of P. mugo in SK–HTMts. It may suggest low phytotoxicity of given MOD or efficient resistance of P. mugo against oxidative stress. In addition, the visible injury index (VINX) covering the broad effect of biotic and abiotic harmful agents was appraised on P. mugo. Percentage of affected surface indicated moderate deterioration of needle injury at the end of the growing season, particularly due to traces of mechanical damage. |
Adamčíková, K; Jánošíková, Z; Pažitný, J Inhibition effect of selected inorganic metal ions on the mycelial growth of Cryphonectria parasitica Journal Article Journal of Plant Protection Research, 60 (4), pp. 399-405, 2020, ISSN: 1427-4345. @article{Adamčíková2020, title = {Inhibition effect of selected inorganic metal ions on the mycelial growth of Cryphonectria parasitica}, author = {K. Adamčíková and Z. Jánošíková and J. Pažitný}, doi = {DOI: 10.24425/jppr.2020.134915}, issn = {1427-4345}, year = {2020}, date = {2020-12-02}, journal = {Journal of Plant Protection Research}, volume = {60}, number = {4}, pages = {399-405}, abstract = {In the current study the antifungal activity of inorganic reagents was tested against Cryphonectria parasitica in vitro in a mycelial growth inhibition test. Three reagents, each consisting of chloride silver (AgCl) in combination with (1) aluminum oxide, (2) zinc oxide − ZnO, and (3) aluminum oxide and titanium dioxide were tested. Significant differences of the tested reagents on the growth of C. parasitica were recorded. The study demonstrated that silver in mixture with ZnO had an antifungal effect and significantly reduced the mycelial growth of C. parasitica in vitro. The mixture of AgCl with the other two combinations of inorganic metal oxides had no inhibition effect on the growth of the pathogen. It was confirmed that ZnO (applied in a single compound test) is responsible for inhibition of C. parasitica mycelium growth. A preliminary in planta assay was performed but statistically significant differences were not recorded in the average increment of canker length.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the current study the antifungal activity of inorganic reagents was tested against Cryphonectria parasitica in vitro in a mycelial growth inhibition test. Three reagents, each consisting of chloride silver (AgCl) in combination with (1) aluminum oxide, (2) zinc oxide − ZnO, and (3) aluminum oxide and titanium dioxide were tested. Significant differences of the tested reagents on the growth of C. parasitica were recorded. The study demonstrated that silver in mixture with ZnO had an antifungal effect and significantly reduced the mycelial growth of C. parasitica in vitro. The mixture of AgCl with the other two combinations of inorganic metal oxides had no inhibition effect on the growth of the pathogen. It was confirmed that ZnO (applied in a single compound test) is responsible for inhibition of C. parasitica mycelium growth. A preliminary in planta assay was performed but statistically significant differences were not recorded in the average increment of canker length. |
Ondrušková, Emília; Ostrovský, Radovan; Jánošíková, Zuzana; Adamčíková, Katarína; Kobza, Marek Selected climatic variables in Slovakia are favourable to the development of Dothistroma needle blight Journal Article Folia Oecologica, 47 (2), pp. 144-152, 2020. @article{Ondrušková2020b, title = {Selected climatic variables in Slovakia are favourable to the development of Dothistroma needle blight}, author = {Emília Ondrušková and Radovan Ostrovský and Zuzana Jánošíková and Katarína Adamčíková and Marek Kobza}, url = {http://ife.sk/wp-content/uploads/2020/09/foecol-2020-0008.pdf}, doi = {10.2478/foecol-2020-0017}, year = {2020}, date = {2020-11-24}, journal = {Folia Oecologica}, volume = {47}, number = {2}, pages = {144-152}, abstract = {Dothistroma needle blight (DNB) is a disease of pine needles. It causes significant defoliation of affected trees; intensive attacks lead to damages of the whole stand. The relationship of trends in disease severity and intensity with climatic variables were studied in three Austrian pine plantations (Jahodná, Kálnica, Litava) during 2014–2018. During the monitoring period, the greatest variability in disease severity was observed in the top third of the crowns, which showed the highest correlation with the variants of the most important climatic conditions (temperature and relative humidity) tested. For the spread of DNB, based on statistical assessment, a higher number of intervals of environmentally favourable climatic conditions is crucial. Both Dothistroma species (D. septosporum and D. pini), which are causal agents of the disease, were identified in Jahodná. In Kálnica and Litava, only D. septosporum was present.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a disease of pine needles. It causes significant defoliation of affected trees; intensive attacks lead to damages of the whole stand. The relationship of trends in disease severity and intensity with climatic variables were studied in three Austrian pine plantations (Jahodná, Kálnica, Litava) during 2014–2018. During the monitoring period, the greatest variability in disease severity was observed in the top third of the crowns, which showed the highest correlation with the variants of the most important climatic conditions (temperature and relative humidity) tested. For the spread of DNB, based on statistical assessment, a higher number of intervals of environmentally favourable climatic conditions is crucial. Both Dothistroma species (D. septosporum and D. pini), which are causal agents of the disease, were identified in Jahodná. In Kálnica and Litava, only D. septosporum was present. |
Barta, Marek; Takov, Danail; Pilarska, Daniela; Doychev, Danail; Horáková, Miriam Kádasi Journal of Forest Science, 66 (10), pp. 420-435, 2020, ISSN: 1212-4834. @article{Barta2020b, title = {Entomopathogenic fungi of the genus Beauveria and their pathogenicity to Ips typographus (Coleoptera: Curculionidae) in the Vitosha National Park, Bulgaria}, author = {Marek Barta and Danail Takov and Daniela Pilarska and Danail Doychev and Miriam Kádasi Horáková}, url = {https://www.agriculturejournals.cz/web/jfs.htm?type=article&id=123_2020-JFS}, doi = {10.17221/123/2020-JFS}, issn = {1212-4834}, year = {2020}, date = {2020-10-01}, journal = {Journal of Forest Science}, volume = {66}, number = {10}, pages = {420-435}, abstract = {Ips typographus is a serious pest for forestry in Eurasia. Effective control is difficult due to its cryptic habits and insect pathogenic microorganisms, including entomopathogenic fungi that are believed to be a promising alternative to the traditional control measures of this pest. In 2018, diversity of entomopathogenic fungi of the genus Beauveria was studied in populations of I. typographus in the Vitosha National Park, Bulgaria. Two species, B. bassiana and B. caledonica, were identified and 33 in vitro strains were obtained. Phylogenetic positions of the strains were evaluated according to phylogenetic inferences based on ITS and TEF-1α. Pathogenicity of the strains against bark beetles was tested in laboratory. All strains were pathogenic, although there was some variability in the efficacy of B. bassiana strains. Virulence of the five most pathogenic strains (four B. bassiana strains and one B. caledonica strain) was compared with the commercial mycoinsecticide Boverol® and highly-virulent B. bassiana strain ARSEF 12957 isolated from I. typographus in Slovakia. The strain from Boverol® was least virulent and the Slovak strain ARSEF 12957 was more efficient than the Bulgarian strains, but the difference was not significant. The laboratory experiments suggest that the Bulgarian strains have a potential for the control of bark beetle adults.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ips typographus is a serious pest for forestry in Eurasia. Effective control is difficult due to its cryptic habits and insect pathogenic microorganisms, including entomopathogenic fungi that are believed to be a promising alternative to the traditional control measures of this pest. In 2018, diversity of entomopathogenic fungi of the genus Beauveria was studied in populations of I. typographus in the Vitosha National Park, Bulgaria. Two species, B. bassiana and B. caledonica, were identified and 33 in vitro strains were obtained. Phylogenetic positions of the strains were evaluated according to phylogenetic inferences based on ITS and TEF-1α. Pathogenicity of the strains against bark beetles was tested in laboratory. All strains were pathogenic, although there was some variability in the efficacy of B. bassiana strains. Virulence of the five most pathogenic strains (four B. bassiana strains and one B. caledonica strain) was compared with the commercial mycoinsecticide Boverol® and highly-virulent B. bassiana strain ARSEF 12957 isolated from I. typographus in Slovakia. The strain from Boverol® was least virulent and the Slovak strain ARSEF 12957 was more efficient than the Bulgarian strains, but the difference was not significant. The laboratory experiments suggest that the Bulgarian strains have a potential for the control of bark beetle adults. |
Barta, Marek; Horáková, Miriam Kádasi; Georgieva, Margarita; Mirchev, Plamen; Zaemdzhikova, Gergana; Pilarska, Daniela; Takov, Danail; Todorov, Milcho; Hubenov, Zdravko; Pilarski, Plamen; Georgiev, Georgi Acta Zoologica Bulgarica, Supplementum 15 , pp. 89-96, 2020, ISSN: 0324-0770. @article{Barta2020, title = {Entomopathogenic Fungi (Ascomycota: Hypocreales) as Natural Antagonists of the Pine Processionary Moth Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae) in Bulgaria}, author = {Marek Barta and Miriam Kádasi Horáková and Margarita Georgieva and Plamen Mirchev and Gergana Zaemdzhikova and Daniela Pilarska and Danail Takov and Milcho Todorov and Zdravko Hubenov and Plamen Pilarski and Georgi Georgiev}, url = {http://www.acta-zoologica-bulgarica.eu/Suppl_15_19.pdf}, issn = {0324-0770}, year = {2020}, date = {2020-08-01}, journal = {Acta Zoologica Bulgarica}, volume = {Supplementum 15}, pages = {89-96}, abstract = {Thaumetopoea pityocampa, a moth naturally distributed in pine ecosystems of Mediterranean region, is considered the most dangerous defoliator in Bulgarian pine forests. Caterpillars of this species also represent a health hazard because their hairs, which contain an urticating protein – thaumetopoein, are responsible for painful skin irritations, rashes and allergic reactions in some people. Therefore, T. pityocampa is not only a serious forest pest, but also a public health problem. Populations of the moth are regulated by a complex of natural enemies. There is only limited information about a parasitic activity of entomopathogenic fungi. These fungi are natural antagonists of insects helping to control of host population and prevention of outbreaks formation. A goal of the present study was to identify species of entomopathogenic fungi in natural populations of T. pityocampa in Bulgaria. During the study, 908 larvae and pupae were collected from two sites (Fotinovo and Kandilka villages) in the south-eastern Rhodopes. In laboratory conditions, 147 larvae or pupae showing characteristic symptoms of mycoses were observed and on 27 of them in vitro isolates were obtained. The cultures were microscopically identified and subsequently analysed by a sequencing study of the internal transcribed region of rDNA and a partial sequence of TEF1-α gene. Altogether, two Beauveria species (B. pseudobassiana – 7 isolates and B. varroae – 6 isolates) and Purpureocillium lilacinum (14 isolates) were identified. The three entomopathogens are reported from T. pityocampa for the first time.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Thaumetopoea pityocampa, a moth naturally distributed in pine ecosystems of Mediterranean region, is considered the most dangerous defoliator in Bulgarian pine forests. Caterpillars of this species also represent a health hazard because their hairs, which contain an urticating protein – thaumetopoein, are responsible for painful skin irritations, rashes and allergic reactions in some people. Therefore, T. pityocampa is not only a serious forest pest, but also a public health problem. Populations of the moth are regulated by a complex of natural enemies. There is only limited information about a parasitic activity of entomopathogenic fungi. These fungi are natural antagonists of insects helping to control of host population and prevention of outbreaks formation. A goal of the present study was to identify species of entomopathogenic fungi in natural populations of T. pityocampa in Bulgaria. During the study, 908 larvae and pupae were collected from two sites (Fotinovo and Kandilka villages) in the south-eastern Rhodopes. In laboratory conditions, 147 larvae or pupae showing characteristic symptoms of mycoses were observed and on 27 of them in vitro isolates were obtained. The cultures were microscopically identified and subsequently analysed by a sequencing study of the internal transcribed region of rDNA and a partial sequence of TEF1-α gene. Altogether, two Beauveria species (B. pseudobassiana – 7 isolates and B. varroae – 6 isolates) and Purpureocillium lilacinum (14 isolates) were identified. The three entomopathogens are reported from T. pityocampa for the first time. |
Drenkhan, R; Ganley, B; Martín-García, J; Vahalík, P; Adamson, K; Adamčíková, K; Ahumada, R; Blank, L; Bragança, H; Capretti, P; Cleary, M; Cornejo, C; Davydenko, K; Diez, J J; Lehtijärvi, Doğmuş H T; Dvořák, M; Enderle, R; Fourie, G; Georgieva, M; Ghelardini, L; Hantula, J; Ioos, R; Iturritxa, E; Kanetis, L; Karpun, N N; Koltay, A; Landeras, E; Markovskaja, S; Mesanza, N; Milenković, I; Musolin, D L; Nikolaou, K; Nowakowska, J A; Ogris, N; Oskay, F; Oszako, T; Papazova-Anakieva, I; Paraschiv, M; Pasquali, M; Pecori, F; Rafoss, T; Raitelaitytė, K; Raposo, R; Robin, C; Rodas, C A; A., Santini A; Sanz-Ros, A V; Selikhovkin, A V; Solla, A; Soukainen, M; Soulioti, N; Steenkamp, E T; Tsopelas, P; Vemić, A; Vettraino, A M; Wingfield, M J; Woodward, S; C., Zamora-Ballesteros C; Mullett, M S Global Geographic Distribution and Host Range of Fusarium circinatum, the Causal Agent of Pine Pitch Canker Journal Article Forests, 11 (7), 2020, ISSN: 1999-4907. @article{Drenkhan2020, title = {Global Geographic Distribution and Host Range of Fusarium circinatum, the Causal Agent of Pine Pitch Canker}, author = {R. Drenkhan and B. Ganley and J. Martín-García and P. Vahalík and K. Adamson and K. Adamčíková and R. Ahumada and L. Blank and H. Bragança and P. Capretti and M. Cleary and C. Cornejo and K. Davydenko and J.J. Diez and H.T. Doğmuş Lehtijärvi and M. Dvořák and R. Enderle and G. Fourie and M. Georgieva and L. Ghelardini and J. Hantula and R. Ioos and E. Iturritxa and L. Kanetis and N.N. Karpun and A. Koltay and E. Landeras and S. Markovskaja and N. Mesanza and I. Milenković and D.L. Musolin and K. Nikolaou and J.A. Nowakowska and N. Ogris and F. Oskay and T. Oszako and I. Papazova-Anakieva and M. Paraschiv and M. Pasquali and F. Pecori and T. Rafoss and K. Raitelaitytė and R. Raposo and C. Robin and C.A. Rodas and A. Santini A. and A.V. Sanz-Ros and A.V. Selikhovkin and A. Solla and M. Soukainen and N. Soulioti and E.T. Steenkamp and P. Tsopelas and A. Vemić and A.M. Vettraino and M.J. Wingfield and S. Woodward and C. Zamora-Ballesteros C. and M.S. Mullett }, doi = {doi:10.3390/f11070724}, issn = {1999-4907}, year = {2020}, date = {2020-07-01}, journal = {Forests}, volume = {11}, number = {7}, abstract = {Fusarium circinatum, the causal agent of pine pitch canker (PPC), is currently one of the most important threats of Pinus spp. globally. This pathogen is known in many pine-growing regions, including natural and planted forests, and can affect all life stages of trees, from emerging seedlings to mature trees. Despite the importance of PPC, the global distribution of F. circinatum is poorly documented, and this problem is also true of the hosts within countries that are affected. The aim of this study was to review the global distribution of F. circinatum, with a particular focus on Europe. We considered (1) the current and historical pathogen records, both positive and negative, based on confirmed reports from Europe and globally; (2) the genetic diversity and population structure of the pathogen; (3) the current distribution of PPC in Europe, comparing published models of predicted disease distribution; and (4) host susceptibility by reviewing literature and generating a comprehensive list of known hosts for the fungus. These data were collated from 41 countries and used to compile a specially constructed geo-database (http://bit.do/phytoportal). A review of 6297 observation records showed that F. circinatum and the symptoms it causes on conifers occurred in 14 countries, including four in Europe, and is absent in 28 countries. Field observations and experimental data from 138 host species revealed 106 susceptible host species including 85 Pinus species, 6 non-pine tree species and 15 grass and herb species. Our data confirm that susceptibility to F. circinatum varies between different host species, tree ages and environmental characteristics. Knowledge on the geographic distribution, host range and the relative susceptibility of different hosts is essential for disease management, mitigation and containment strategies. The findings reported in this review will support countries that are currently free of F. circinatum in implementing effective procedures and restrictions and prevent further spread of the pathogen.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fusarium circinatum, the causal agent of pine pitch canker (PPC), is currently one of the most important threats of Pinus spp. globally. This pathogen is known in many pine-growing regions, including natural and planted forests, and can affect all life stages of trees, from emerging seedlings to mature trees. Despite the importance of PPC, the global distribution of F. circinatum is poorly documented, and this problem is also true of the hosts within countries that are affected. The aim of this study was to review the global distribution of F. circinatum, with a particular focus on Europe. We considered (1) the current and historical pathogen records, both positive and negative, based on confirmed reports from Europe and globally; (2) the genetic diversity and population structure of the pathogen; (3) the current distribution of PPC in Europe, comparing published models of predicted disease distribution; and (4) host susceptibility by reviewing literature and generating a comprehensive list of known hosts for the fungus. These data were collated from 41 countries and used to compile a specially constructed geo-database (http://bit.do/phytoportal). A review of 6297 observation records showed that F. circinatum and the symptoms it causes on conifers occurred in 14 countries, including four in Europe, and is absent in 28 countries. Field observations and experimental data from 138 host species revealed 106 susceptible host species including 85 Pinus species, 6 non-pine tree species and 15 grass and herb species. Our data confirm that susceptibility to F. circinatum varies between different host species, tree ages and environmental characteristics. Knowledge on the geographic distribution, host range and the relative susceptibility of different hosts is essential for disease management, mitigation and containment strategies. The findings reported in this review will support countries that are currently free of F. circinatum in implementing effective procedures and restrictions and prevent further spread of the pathogen. |
Jančovičová, S; Adamčíková, K; Caboň, M; Adamčík, S How variable is Crepidotus variabilis? Journal Article Phytotaxa, 449 (3), pp. 243-264, 2020, ISSN: 1179-3155. @article{Jančovičová2020, title = {How variable is Crepidotus variabilis?}, author = {S. Jančovičová and K. Adamčíková and M. Caboň and S. Adamčík}, url = {http://ife.sk/wp-content/uploads/2016/10/fig-phytotaxa-sona-300x206.jpg}, doi = {doi.org/10.11646/phytotaxa.449.3.4}, issn = {1179-3155}, year = {2020}, date = {2020-06-23}, journal = {Phytotaxa}, volume = {449}, number = {3}, pages = {243-264}, abstract = {This study evaluates the morphological concept of Crepidotus variabilis and similar taxa based on basidiospore and cheilocystidia characters. A phylogenetic analysis of the ITS region clearly distinguished C. variabilis var. variabilis from the two other distinct taxa of the same lineage, C. variabilis var. trichocystis and C. neotrichocystis. We also recognised one additional morphologically similar and undescribed species that belongs to the C. variabilis lineage. All four taxa can also be recognised morphologically using basidiospore and cheilocystidia characters. Our detailed statistical comparison of these characters improves the knowledge of their variability and specifies more precisely the morphological circumscription of the taxa within this group.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study evaluates the morphological concept of Crepidotus variabilis and similar taxa based on basidiospore and cheilocystidia characters. A phylogenetic analysis of the ITS region clearly distinguished C. variabilis var. variabilis from the two other distinct taxa of the same lineage, C. variabilis var. trichocystis and C. neotrichocystis. We also recognised one additional morphologically similar and undescribed species that belongs to the C. variabilis lineage. All four taxa can also be recognised morphologically using basidiospore and cheilocystidia characters. Our detailed statistical comparison of these characters improves the knowledge of their variability and specifies more precisely the morphological circumscription of the taxa within this group. |
Pastirčáková, K; Adamčíková, K; Barta, M; Pažitný, J; Hoťka, P; Sarvašová, I; Kádasi-Horáková, M Host range of Hymenoscyphus fraxineus in Slovak arboreta Journal Article Forests, 11 (5), pp. 1-18, Article Number: 596, 2020, ISSN: 1999-4907. @article{Pastirčáková25.0, title = {Host range of \textit{Hymenoscyphus fraxineus} in Slovak arboreta}, author = {K. Pastirčáková and K. Adamčíková and M. Barta and J. Pažitný and P. Hoťka and I. Sarvašová and M. Kádasi-Horáková}, doi = {10.3390/f11050596}, issn = {1999-4907}, year = {2020}, date = {2020-05-25}, journal = {Forests}, volume = {11}, number = {5}, pages = {1-18, Article Number: 596}, abstract = {The health of 34 different Fraxinus taxa in association with the pathogenic fungus Hymenoscyphus fraxineus was assessed in four Slovak arboreta. Averaged across all arboreta, nearly one-quarter (24.9%) of all evaluated trees showed ash dieback symptoms. The damage was most serious on the common ash F. excelsior, a native species. The percentage of dead trees did not exceed 2% for all evaluated trees. Generally, ash trees of all ages were affected, though the intensity of the damage varied among the sites. The identity of H. fraxineus was confirmed by conventional PCR targeting the internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA, as well as the 18S gene/ITS-2 region of the rDNA operon. In Slovakia, the pathogen has expanded its host range from native species not only to their ornamental cultivars, but also to introduced North American (F. cinerea, F. latifolia, F. pennsylvanica, F. quadrangulata) and Asian (F. bungeana, F. chinensis ssp. rhynchophylla, F. mandshurica) ash species. H. fraxineus was also observed on the previous year’s leaf petioles of the native European species F. ornus, considered a weakly susceptible host. In Slovak arboreta, H. fraxineus was found on 23 Fraxinus taxa; 21 of them represent first records for the country. F. bungeana is recorded as a new host species of H. fraxineus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The health of 34 different Fraxinus taxa in association with the pathogenic fungus Hymenoscyphus fraxineus was assessed in four Slovak arboreta. Averaged across all arboreta, nearly one-quarter (24.9%) of all evaluated trees showed ash dieback symptoms. The damage was most serious on the common ash F. excelsior, a native species. The percentage of dead trees did not exceed 2% for all evaluated trees. Generally, ash trees of all ages were affected, though the intensity of the damage varied among the sites. The identity of H. fraxineus was confirmed by conventional PCR targeting the internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA, as well as the 18S gene/ITS-2 region of the rDNA operon. In Slovakia, the pathogen has expanded its host range from native species not only to their ornamental cultivars, but also to introduced North American (F. cinerea, F. latifolia, F. pennsylvanica, F. quadrangulata) and Asian (F. bungeana, F. chinensis ssp. rhynchophylla, F. mandshurica) ash species. H. fraxineus was also observed on the previous year’s leaf petioles of the native European species F. ornus, considered a weakly susceptible host. In Slovak arboreta, H. fraxineus was found on 23 Fraxinus taxa; 21 of them represent first records for the country. F. bungeana is recorded as a new host species of H. fraxineus. |
Rusín, Jiří; Mudrončeková, Silvia; Barta, Marek; Chamrádová, Kateřina Spruce bark beetles as a biological waste that can be processed in a biogas plant Journal Article Turkish Journal of Agriculture and Forestry, 44 (2), pp. 169-179, 2020, ISSN: 1300-011X. @article{Rusín2020, title = {Spruce bark beetles as a biological waste that can be processed in a biogas plant}, author = {Jiří Rusín and Silvia Mudrončeková and Marek Barta and Kateřina Chamrádová}, url = {https://journals.tubitak.gov.tr/agriculture/abstract.htm?id=26786}, doi = {10.3906/tar-1902-13}, issn = {1300-011X}, year = {2020}, date = {2020-04-01}, journal = {Turkish Journal of Agriculture and Forestry}, volume = {44}, number = {2}, pages = {169-179}, abstract = {Biogas plays an important role in reducing the amount of biowaste that is converted to methane which is usable by multiple pathways in the energy sector. Bark beetles are among the most important pests of spruce forests in Europe. Principal measures of managing bark beetle populations include trapping them with pheromone traps. Biomass of bark beetles collected by the traps constitutes a waste of forest resources that could potentially be processed in biogas plants. The aim of this study was to provide information about the trapping of bark beetles by pheromone traps in Tatra National Park during 2005–2018 and to verify the energy potential of a combination of liquid digestate and bark beetle biomass collected by pheromone traps. The bark beetle biomass is a seasonal small volume nitrogen-rich material which could be processed at agricultural biogas plants. The biochemical methane potential batch test performed at 40 °C showed a high degree of digestion of milled beetles within 40 days, with a methane production of 0.242 Nm3 per kilogram of total solids (TS). The single-stage low-solids mesophilic fed-batch anaerobic digestion of untreated beetles was carried out in a lab-scale rotary drum reactor. Within 52 days, the average organic loading rate was 1.019 kg VS m–3 day–1, where VS stands for volatile solids. The specific biogas and methane production reached 0.218 Nm3 kg–1 TS and 0.140 Nm3 kg–1 TS, respectively. The cuticles of the beetle bodies were not obviously disturbed by the fermentation process. The digestate was quite rich in nitrogen, but the other element contents did not differ too much from fertilizer made from manure or phytomass.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Biogas plays an important role in reducing the amount of biowaste that is converted to methane which is usable by multiple pathways in the energy sector. Bark beetles are among the most important pests of spruce forests in Europe. Principal measures of managing bark beetle populations include trapping them with pheromone traps. Biomass of bark beetles collected by the traps constitutes a waste of forest resources that could potentially be processed in biogas plants. The aim of this study was to provide information about the trapping of bark beetles by pheromone traps in Tatra National Park during 2005–2018 and to verify the energy potential of a combination of liquid digestate and bark beetle biomass collected by pheromone traps. The bark beetle biomass is a seasonal small volume nitrogen-rich material which could be processed at agricultural biogas plants. The biochemical methane potential batch test performed at 40 °C showed a high degree of digestion of milled beetles within 40 days, with a methane production of 0.242 Nm3 per kilogram of total solids (TS). The single-stage low-solids mesophilic fed-batch anaerobic digestion of untreated beetles was carried out in a lab-scale rotary drum reactor. Within 52 days, the average organic loading rate was 1.019 kg VS m–3 day–1, where VS stands for volatile solids. The specific biogas and methane production reached 0.218 Nm3 kg–1 TS and 0.140 Nm3 kg–1 TS, respectively. The cuticles of the beetle bodies were not obviously disturbed by the fermentation process. The digestate was quite rich in nitrogen, but the other element contents did not differ too much from fertilizer made from manure or phytomass. |
Buyck, B; Wang, X H; Adamčíková, K; Caboň, M; Jančovičová, S; Hofstetter, V; Adamčík, S One step closer to unravelling the origin of Russula: subgenus Glutinosae subg. nov. Journal Article Mycosphere, 11 (1), pp. 285–304, 2020, ISSN: 2077 7019. @article{Buyck2020, title = {One step closer to unravelling the origin of Russula: subgenus Glutinosae subg. nov.}, author = {B. Buyck and X. H. Wang and K. Adamčíková and M. Caboň and S Jančovičová and V. Hofstetter and S. Adamčík }, doi = {Doi 10.5943/mycosphere/11/1/6}, issn = {2077 7019}, year = {2020}, date = {2020-01-31}, journal = {Mycosphere}, volume = {11}, number = {1}, pages = {285–304}, abstract = {This study reports on the discovery of a new subgenus, Russula subg. Glutinosae, having an Eastern North American – East Asian distribution. A multigene phylogeny places this new subgenus sister with strong support to a well-supported clade composed of subgenera Compactae and Archaeae. It holds only two very rare, northern hemisphere species, the North American R. glutinosa and the Asian R. glutinosoides sp. nov., thereby adding support to a northern hemisphere origin of the genus. Russula fattoensis is here shown to be a synonym of R. glutinosa. Detailed morphological descriptions and illustrations of holotype collections are provided and potential affinities and similarities with other subgenera are discussed. The new subgenus is a perfect illustration of the fact that nBLAST of nrITS does not always provide the appropriate sampling for phylogenetic analyses.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study reports on the discovery of a new subgenus, Russula subg. Glutinosae, having an Eastern North American – East Asian distribution. A multigene phylogeny places this new subgenus sister with strong support to a well-supported clade composed of subgenera Compactae and Archaeae. It holds only two very rare, northern hemisphere species, the North American R. glutinosa and the Asian R. glutinosoides sp. nov., thereby adding support to a northern hemisphere origin of the genus. Russula fattoensis is here shown to be a synonym of R. glutinosa. Detailed morphological descriptions and illustrations of holotype collections are provided and potential affinities and similarities with other subgenera are discussed. The new subgenus is a perfect illustration of the fact that nBLAST of nrITS does not always provide the appropriate sampling for phylogenetic analyses. |
Adamčík, S; Dima, B; Adamčíková, K; Corriol, G; Læssøe, T; Moreau, P -A; Caboň, M; Jančovičová, S Hodophilus phaeophyllus complex (Clavariaceae, Agaricales) is defined as new phylogenetic lineage in Europe Journal Article Mycological Progress, 19 (2), pp. 111-125, 2020, ISSN: 1617-416X. @article{Adamčík2020, title = {Hodophilus phaeophyllus complex (Clavariaceae, Agaricales) is defined as new phylogenetic lineage in Europe}, author = {S. Adamčík and B. Dima and K. Adamčíková and G. Corriol and T. Læssøe and P.-A. Moreau and M. Caboň and S. Jančovičová }, doi = {DOI 10.1007/s11557-019-01544-9}, issn = {1617-416X}, year = {2020}, date = {2020-01-30}, journal = {Mycological Progress}, volume = {19}, number = {2}, pages = {111-125}, abstract = {The study deals with the last unexplored morphological group of the genus Hodophilus defined by absence of distinct odours, absence of yellow colours and absence of darker dots on the stipe. The phylogenetic reconstruction of the whole genus based on nrITS, nrLSU and RPB2 sequences placed all European members having these morphological characters in a monophyletic group defined here as a new section H. sect. Phaeophylli. The remaining European members of the genus are placed in two additional groups classified as section H. sect. Hodophilus and the new section H. sect. Micacei. Five species are recognised within section Phaeophylli which is typified by H. phaeophyllus that is lecto- and epitypified. Three new species belonging to this section are described: H. carpathicus, H. decurrentior and H. stramineus. Hodophilus decurrentior is the only species showing distinct morphological differences under the microscope. The identification of other species of the section depends mainly on the colour of basidiomata. An updated key to all European members of the genus is provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The study deals with the last unexplored morphological group of the genus Hodophilus defined by absence of distinct odours, absence of yellow colours and absence of darker dots on the stipe. The phylogenetic reconstruction of the whole genus based on nrITS, nrLSU and RPB2 sequences placed all European members having these morphological characters in a monophyletic group defined here as a new section H. sect. Phaeophylli. The remaining European members of the genus are placed in two additional groups classified as section H. sect. Hodophilus and the new section H. sect. Micacei. Five species are recognised within section Phaeophylli which is typified by H. phaeophyllus that is lecto- and epitypified. Three new species belonging to this section are described: H. carpathicus, H. decurrentior and H. stramineus. Hodophilus decurrentior is the only species showing distinct morphological differences under the microscope. The identification of other species of the section depends mainly on the colour of basidiomata. An updated key to all European members of the genus is provided. |
Elvira-Recuenco, M; Cacciola, S O; Sanz-Ros, A V; Garbelotto, M; Aguayo, J; Solla, A; Mullett, M; Drenkhan, T; Oskay, F; Kaya, A G A; Iturritxa, E; Cleary, M; Witzell, J; Georgieva, M; Papazova-Anakieva, I; Chira, D; Paraschiv, M; Musolin, D L; Selikhovkin, A V; Varentsova, Yu. E; Adamčíková, K; Markovskaja, S; Mesanza, N; Davydenko, K; Capretti, P; Scanu, B; Gonthier, P; Tsopelas, P; Martín-García, J; Morales-Rodríguez, C; Lehtijärvi, A; Lehtijärvi, Doğmuş H T; Oszako, T; Nowakowska, J A; Bragança, H; Fernández-Fernández, M; Hantula, J; Díez, J J Potential Interactions Between Invasive Fusarium circinatum and Other Pine Pathogens in Europe Journal Article Forests, 11 (1), 2020, ISSN: 1999-4907. @article{Elvira-Recuenco2019, title = {Potential Interactions Between Invasive Fusarium circinatum and Other Pine Pathogens in Europe}, author = {M. Elvira-Recuenco and S. O. Cacciola and A. V. Sanz-Ros and M. Garbelotto and J. Aguayo and A. Solla and M. Mullett and T. Drenkhan and F. Oskay and A. G. A. Kaya and E. Iturritxa and M. Cleary and J. Witzell and M. Georgieva and I. Papazova-Anakieva and D. Chira and M. Paraschiv and D.L. Musolin and A.V. Selikhovkin and E. Yu. Varentsova and K. Adamčíková and S. Markovskaja and N. Mesanza and K. Davydenko and P. Capretti and B. Scanu and P. Gonthier and P. Tsopelas and J. Martín-García and C. Morales-Rodríguez and A. Lehtijärvi and H. T. Doğmuş Lehtijärvi and T. Oszako and J.A. Nowakowska and H. Bragança and M. Fernández-Fernández and J. Hantula and J.J. Díez }, doi = {doi:10.3390/f11010007}, issn = {1999-4907}, year = {2020}, date = {2020-01-18}, journal = {Forests}, volume = {11}, number = {1}, abstract = {Pines are major components of native forests and plantations in Europe, where they have both economic significance and an important ecological role. Diseases of pines are mainly caused by fungal and oomycete pathogens, and can significantly reduce the survival, vigor, and yield of both individual trees and entire stands or plantations. Pine pitch canker (PPC), caused by Fusarium circinatum (Nirenberg and O’Donnell), is among the most devastating pine diseases in the world, and is an example of an emergent invasive disease in Europe. The effects of microbial interactions on plant health, as well as the possible roles plant microbiomes may have in disease expression, have been the focus of several recent studies. Here, we describe the possible effects of co-infection with pathogenic fungi and oomycetes with F. circinatum on the health of pine seedlings and mature plants, in an attempt to expand our understanding of the role that biotic interactions may play in the future of PPC disease in European nurseries and forests. The available information on pine pathogens that are able to co-occur with F. circinatum in Europe is here reviewed and interpreted to theoretically predict the effects of such co-occurrences on pine survival, growth, and yield. Beside the awareness that F. circinatum may co-occurr on pines with other pathogens, an additional outcome from this review is an updating of the literature, including the so-called grey literature, to document the geographical distribution of the relevant pathogens and to facilitate differential diagnoses, particularly in nurseries, where some of them may cause symptoms similar to those induced by F. circinatum. An early and accurate diagnosis of F. circinatum, a pathogen that has been recently introduced and that is currently regulated in Europe, is essential to prevent its introduction and spread in plantings and forests.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Pines are major components of native forests and plantations in Europe, where they have both economic significance and an important ecological role. Diseases of pines are mainly caused by fungal and oomycete pathogens, and can significantly reduce the survival, vigor, and yield of both individual trees and entire stands or plantations. Pine pitch canker (PPC), caused by Fusarium circinatum (Nirenberg and O’Donnell), is among the most devastating pine diseases in the world, and is an example of an emergent invasive disease in Europe. The effects of microbial interactions on plant health, as well as the possible roles plant microbiomes may have in disease expression, have been the focus of several recent studies. Here, we describe the possible effects of co-infection with pathogenic fungi and oomycetes with F. circinatum on the health of pine seedlings and mature plants, in an attempt to expand our understanding of the role that biotic interactions may play in the future of PPC disease in European nurseries and forests. The available information on pine pathogens that are able to co-occur with F. circinatum in Europe is here reviewed and interpreted to theoretically predict the effects of such co-occurrences on pine survival, growth, and yield. Beside the awareness that F. circinatum may co-occurr on pines with other pathogens, an additional outcome from this review is an updating of the literature, including the so-called grey literature, to document the geographical distribution of the relevant pathogens and to facilitate differential diagnoses, particularly in nurseries, where some of them may cause symptoms similar to those induced by F. circinatum. An early and accurate diagnosis of F. circinatum, a pathogen that has been recently introduced and that is currently regulated in Europe, is essential to prevent its introduction and spread in plantings and forests. |
2019 |
Adamčík, S; Looney, B; Caboň, M; Jančovičová, S; Adamčíková, K; Avis, P G; Barajas, M; Bhatt, R P; Corrales, A; Das, K; Hampe, F; Ghosh, A; Gates, G; Kälviäinen, V; Khalid, A N; Kiran, M; Lange, De R; Lee, H; Lim, Y W; Kong, A; Manz, C; Ovrebo, C; Saba, M; Taipale, T; Verbeken, A; Wisitrassameewong, K; Buyck, B The quest for a globally comprehensible Russula language Journal Article Fungal Diversity, 99 (1), pp. 369-449, 2019, ISSN: 1560-2745. @article{Adamčík2019, title = {The quest for a globally comprehensible Russula language}, author = {S. Adamčík and B. Looney and M. Caboň and S. Jančovičová and K. Adamčíková and P. G. Avis and M. Barajas and R. P. Bhatt and A. Corrales and K. Das and F. Hampe and A. Ghosh and G. Gates and V. Kälviäinen and A. N. Khalid and M. Kiran and R. De Lange and H. Lee and Y. W. Lim and A. Kong and C. Manz and C. Ovrebo and M. Saba and T. Taipale and A. Verbeken and K. Wisitrassameewong and B. Buyck}, doi = {doi.org/10.1007/s13225-019-00437-2}, issn = {1560-2745}, year = {2019}, date = {2019-10-24}, journal = {Fungal Diversity}, volume = {99}, number = {1}, pages = {369-449}, abstract = {Since 2007, the quality of Russula descriptions has improved and the use of molecular support for species delimitation and the number of published new species has increased. However, the description style is not consistent and has regional or author-specific patterns. Most recent publications still favour descriptions of spores compared to hymenium and pileipellis elements, and usually only the spore size is provided with statistical support. This study proposes standards for descriptions of the microscopic structure of Russula species (Russulaceae, Agaricomycetes). We present the description template, the template measurements table, the specific terminology and the essential chemical reagents. The proposed standards were tested by mycologists from 11 countries who met at the Russula Microscopy Workshop in Slovakia. Descriptions of 26 species from 9 countries and four continents were prepared, among them R. amarissima, R. castanopsidis, R. seperina and R. subtilis are re-described and 15 species are introduced as new: R. abietiphila, R. amerorecondita, R. aurantioflava, R. echidna, R. flavobrunnescens, R. fluvialis, R. fortunae, R. garyensis, R. gemmata, R. laevis, R. madrensis, R. olivaceohimalayensis, R. purpureogracilis, R. sancti-pauli and R. wielangtae. Seven descriptions for candidate new species are provided without a formal name assignment. Pairwise comparison of species described in this study with available similar descriptions of related species suggests that microscopic characters from all parts of the basidiomata can be equally important for species recognition and they deserve the same treatment including number of measurements and statistics. The majority of recent studies does not recognise differences between the pileus margin and centre, but more than one-third of the species described in this study show distinct differences between the pileus areas, emphasizing the importance to specify the origin of pileipellis observations. This study proved that there is frequently insufficient difference in the ITS barcode between closely related species and that it is necessary to use more genetic markers combined with ecological and geographical data.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Since 2007, the quality of Russula descriptions has improved and the use of molecular support for species delimitation and the number of published new species has increased. However, the description style is not consistent and has regional or author-specific patterns. Most recent publications still favour descriptions of spores compared to hymenium and pileipellis elements, and usually only the spore size is provided with statistical support. This study proposes standards for descriptions of the microscopic structure of Russula species (Russulaceae, Agaricomycetes). We present the description template, the template measurements table, the specific terminology and the essential chemical reagents. The proposed standards were tested by mycologists from 11 countries who met at the Russula Microscopy Workshop in Slovakia. Descriptions of 26 species from 9 countries and four continents were prepared, among them R. amarissima, R. castanopsidis, R. seperina and R. subtilis are re-described and 15 species are introduced as new: R. abietiphila, R. amerorecondita, R. aurantioflava, R. echidna, R. flavobrunnescens, R. fluvialis, R. fortunae, R. garyensis, R. gemmata, R. laevis, R. madrensis, R. olivaceohimalayensis, R. purpureogracilis, R. sancti-pauli and R. wielangtae. Seven descriptions for candidate new species are provided without a formal name assignment. Pairwise comparison of species described in this study with available similar descriptions of related species suggests that microscopic characters from all parts of the basidiomata can be equally important for species recognition and they deserve the same treatment including number of measurements and statistics. The majority of recent studies does not recognise differences between the pileus margin and centre, but more than one-third of the species described in this study show distinct differences between the pileus areas, emphasizing the importance to specify the origin of pileipellis observations. This study proved that there is frequently insufficient difference in the ITS barcode between closely related species and that it is necessary to use more genetic markers combined with ecological and geographical data. |
Pastirčáková, K In vitro interactions between Trichoderma harzianum and pathogenic fungi damaging horse-chestnut (Aesculus hippocastanum) leaves and fruits Journal Article Biological Letters, 54 (1), pp. 21-35, 2019, ISSN: 1644-7700. @article{Pastirčáková2019, title = {\textit{In vitro} interactions between \textit{Trichoderma harzianum} and pathogenic fungi damaging horse-chestnut (\textit{Aesculus hippocastanum}) leaves and fruits}, author = {K. Pastirčáková}, doi = {10.2478/biolet-2019-0003}, issn = {1644-7700}, year = {2019}, date = {2019-08-06}, journal = {Biological Letters}, volume = {54}, number = {1}, pages = {21-35}, abstract = {Interactions between 3 pathogenic fungi damaging horse-chestnut (Aesculus hippocastanum) leaves and fruits – Phyllosticta sphaeropsoidea, Phomopsis carposchiza, and Diaporthe padi – and the antagonistic fungus Trichoderma harzianum were studied to determine their mutual influence in vitro. Antibiosis of colonies developing on 5 nutrient media was tested. The 3 studied T. harzianum isolates differed in their antagonistic potential. Although T. harzianum isolates significantly inhibited the growth of Phomopsis carposchiza, the mycelium growth of some of the re-isolates on fresh medium indicates an inadequate antagonistic effect of T. harzianum on this species. The tested Trichoderma isolates showed stronger antagonism towards the other pathogens, reflected in overgrowing of Phyllosticta sphaeropsoidea and Diaporthe padi and reducing their growth. Granulation of the cytoplasm and lysis of hyphae of the fungal pathogens were the most frequently observed effects of the interaction.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Interactions between 3 pathogenic fungi damaging horse-chestnut (Aesculus hippocastanum) leaves and fruits – Phyllosticta sphaeropsoidea, Phomopsis carposchiza, and Diaporthe padi – and the antagonistic fungus Trichoderma harzianum were studied to determine their mutual influence in vitro. Antibiosis of colonies developing on 5 nutrient media was tested. The 3 studied T. harzianum isolates differed in their antagonistic potential. Although T. harzianum isolates significantly inhibited the growth of Phomopsis carposchiza, the mycelium growth of some of the re-isolates on fresh medium indicates an inadequate antagonistic effect of T. harzianum on this species. The tested Trichoderma isolates showed stronger antagonism towards the other pathogens, reflected in overgrowing of Phyllosticta sphaeropsoidea and Diaporthe padi and reducing their growth. Granulation of the cytoplasm and lysis of hyphae of the fungal pathogens were the most frequently observed effects of the interaction. |
K. Adamčíková E. Ondrušková, Kobza M Hypovirulence in chestnut blight fungus, Cryphonectria parasitica, in Slovakia Journal Article Biocontrol Science and Technology, 29 (9), pp. 840-851, 2019, ISSN: 0958-3157. @article{Adamčíková2019, title = {Hypovirulence in chestnut blight fungus, Cryphonectria parasitica, in Slovakia}, author = {K. Adamčíková, E. Ondrušková, M. Kobza}, doi = {DOI: 10.1080/09583157.2019.1608509}, issn = {0958-3157}, year = {2019}, date = {2019-07-19}, journal = {Biocontrol Science and Technology}, volume = {29}, number = {9}, pages = {840-851}, abstract = {The recovery of the European chestnut from chestnut blight in Europe is attributed to hypovirulence, a viral disease that reduces the virulence of its host – Cryphonectria parasitica. Hypovirulence has been used in biocontrol programmes throughout Europe with the aim of not only treating individual chestnut blight cankers but causing the natural spread and establishment of viruses in C. parasitica populations. Almost 40 years after the first appearance of chestnut blight and more than 20 years after the first biocontrol treatment implementation in Slovakia the establishment and natural spread of hypovirulence were studied. The morphological characteristics of 179 chestnut blight cankers and 161 C. parasitica strains were evaluated for the presence of hypovirus from three chestnut cultivation sites in Slovakia. Nineteen cankers and isolates were considered hypovirulent based on visual assessment of their morphological characteristics. The hypovirus presence by RNA extraction was confirmed in 6 cankers/strains.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The recovery of the European chestnut from chestnut blight in Europe is attributed to hypovirulence, a viral disease that reduces the virulence of its host – Cryphonectria parasitica. Hypovirulence has been used in biocontrol programmes throughout Europe with the aim of not only treating individual chestnut blight cankers but causing the natural spread and establishment of viruses in C. parasitica populations. Almost 40 years after the first appearance of chestnut blight and more than 20 years after the first biocontrol treatment implementation in Slovakia the establishment and natural spread of hypovirulence were studied. The morphological characteristics of 179 chestnut blight cankers and 161 C. parasitica strains were evaluated for the presence of hypovirus from three chestnut cultivation sites in Slovakia. Nineteen cankers and isolates were considered hypovirulent based on visual assessment of their morphological characteristics. The hypovirus presence by RNA extraction was confirmed in 6 cankers/strains. |
Pažitný, J; Kobza, M; Adamčíková, K Variance in leaf spot susceptibility in chestnut trees of different species and hybrids Journal Article Journal of Plant Disease and Protection, 126 (2), pp. 169-172, 2019, ISSN: 1861-3829. @article{Pažitný2019, title = {Variance in leaf spot susceptibility in chestnut trees of different species and hybrids}, author = {J. Pažitný and M. Kobza and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s41348-019-00209-9}, doi = {DOI 10.1007/s41348-019-00209-9}, issn = {1861-3829}, year = {2019}, date = {2019-02-19}, journal = {Journal of Plant Disease and Protection}, volume = {126}, number = {2}, pages = {169-172}, abstract = {Prior to the first occurrence of chestnut blight in 1976, leaf spot caused by Cylindrosporium castaneae was one of the most important diseases of European chestnut trees in Slovakia. In 2011, extensive damage of leaves by leaf spot was recorded in young chestnut trees in an experimental chestnut orchard in Pribelce, Slovakia, where seedlings and grafts of different taxonomic origin (Castanea sativa, Castanea crenata, and C. sativa × C. crenata) exhibited visible differences in the extent of leaf spot. Digital images of particular leaves were evaluated using Quick Micro Photo software and the ratio of infected leaf area was calculated. Morphological observations of the fungal species causing leaf spot were conducted and the disease symptoms were described and discussed. Taxonomic origin of studied trees showed a significant effect on the proportion of leaf spot area, indicating that the response to fungal attacks has a strong genetic determinism, with resistance being clearly influenced by taxonomic origin. The lowest leaf spot infection was recorded in C. crenata seedlings. Significant differences were noted between the individual trees tested and between the studied years.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Prior to the first occurrence of chestnut blight in 1976, leaf spot caused by Cylindrosporium castaneae was one of the most important diseases of European chestnut trees in Slovakia. In 2011, extensive damage of leaves by leaf spot was recorded in young chestnut trees in an experimental chestnut orchard in Pribelce, Slovakia, where seedlings and grafts of different taxonomic origin (Castanea sativa, Castanea crenata, and C. sativa × C. crenata) exhibited visible differences in the extent of leaf spot. Digital images of particular leaves were evaluated using Quick Micro Photo software and the ratio of infected leaf area was calculated. Morphological observations of the fungal species causing leaf spot were conducted and the disease symptoms were described and discussed. Taxonomic origin of studied trees showed a significant effect on the proportion of leaf spot area, indicating that the response to fungal attacks has a strong genetic determinism, with resistance being clearly influenced by taxonomic origin. The lowest leaf spot infection was recorded in C. crenata seedlings. Significant differences were noted between the individual trees tested and between the studied years. |
2018 |
Brindza, J; Motyleva, S; Ostrovský, R; Grygorieva, O V; Adamchuk, L; Horčinová-Sedláčková, V; Juríková, T; Fatrcová-Šramková, K; Schwarzová, M; Brovarskyi, V; Velichko, S; Tkachenko, O Peľ a včelie peľové obnôžky z niektorých druhov rastlín Book Chapter Brindza, J; Motyleva, S (Ed.): Chapter 4, pp. 147, Slovenská poľnohospodárska univerzita v Nitre, Tr. A. Hlinku 2, 949 01 Nitra, FarmersEduca, 2018, ISBN: 978-80-552-1862-5. @inbook{Brindza2018, title = {Peľ a včelie peľové obnôžky z niektorých druhov rastlín}, author = {J. Brindza and S. Motyleva and R. Ostrovský and O.V. Grygorieva and L. Adamchuk and V. Horčinová-Sedláčková and T. Juríková and K. Fatrcová-Šramková and M. Schwarzová and V. Brovarskyi and S. Velichko and O. Tkachenko}, editor = {J. Brindza and S. Motyleva}, isbn = {978-80-552-1862-5}, year = {2018}, date = {2018-12-17}, pages = {147}, publisher = {Slovenská poľnohospodárska univerzita v Nitre}, address = {Tr. A. Hlinku 2, 949 01 Nitra}, edition = {FarmersEduca}, chapter = {4}, abstract = {Odborná publikácia, v ktorej autori prezentujú niektoré rastlinné druhy, z ktorých včely zbierajú peľové zrná a využívajú ich na formovanie obnôžok. Publikácia poskytuje základné informácie o peli a včelích peľových obnôžkach s prezentáciou na skupine charakterizovaných druhov. Je určená pre malých, mladých a rodinných farmárov, včelárov, pestovateľov rastlín, množiteľov a spravovateľov. Uvedená kapitola predstavuje dominantnú časť publikácie. V kapitole je prezentovaná morfometrická charakteristika peľových zŕn a včelích peľových obnôžok vybranej skupiny 26 rastlinných druhov.}, type = {inbook}, keywords = {}, pubstate = {published}, tppubtype = {inbook} } Odborná publikácia, v ktorej autori prezentujú niektoré rastlinné druhy, z ktorých včely zbierajú peľové zrná a využívajú ich na formovanie obnôžok. Publikácia poskytuje základné informácie o peli a včelích peľových obnôžkach s prezentáciou na skupine charakterizovaných druhov. Je určená pre malých, mladých a rodinných farmárov, včelárov, pestovateľov rastlín, množiteľov a spravovateľov. Uvedená kapitola predstavuje dominantnú časť publikácie. V kapitole je prezentovaná morfometrická charakteristika peľových zŕn a včelích peľových obnôžok vybranej skupiny 26 rastlinných druhov. |
Bolvanský, M; Pažitný, J; Adamčíková, K Grading of blight resistance in different chestnut accessions. Journal Article Acta Horticulturae, 1220 , pp. 87-94, 2018, ISSN: 05677572. @article{M.2018, title = {Grading of blight resistance in different chestnut accessions.}, author = {M. Bolvanský and J. Pažitný and K. Adamčíková}, url = {https://www.actahort.org/members/showpdf?booknrarnr=1220_13}, doi = {10.17660/ActaHortic.2018.1220.13}, issn = {05677572}, year = {2018}, date = {2018-11-01}, journal = {Acta Horticulturae}, volume = {1220}, pages = {87-94}, abstract = {Five chestnut trees (three hybrids Castanea sativa × C. crenata, one C. sativa and one C. crenata) with different levels of blight resistance were subjected to the screening by two virulent and one hypovirulent strains of Cryphonectria parasitica in order to examine reliability of ex situ tests for grading of blight resistance in chestnut. Branches 2-3 cm in diameter were harvested from the trees, in early April and end of September and stored at about 10°C for either 2 weeks (for branch sections) or 4 weeks (for bark- and wood-tissue sections). Branch sections and bark- and wood-tissue sections were inoculated with the C. parasitica strains and incubated in the dark at 25°C with 95% humidity for 4 weeks and/or 4 days, respectively. In branch sections, superficial cankers were very rare so only occurrence of pycnidia formed around the inoculating holes were evaluated. Pycnidia occurred more frequently in branch sections collected from the less resistant accessions in October trial. The size of necrotic areas reflected the resistance ranking of accessions in April/May better than in October inoculation trials. Results of inoculations on excised wood tissue were more consistent with the real blight resistance level than data obtained from inoculations on excised bark tissue. Occasional inconsistence between results of inoculations carried out at different dates or in different trees might be caused by different physiological state of collected branches. Based on these results it can be concluded that the end of dormant period seems to be the most suitable time to carry out both pathogenicity and resistance tests in chestnuts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five chestnut trees (three hybrids Castanea sativa × C. crenata, one C. sativa and one C. crenata) with different levels of blight resistance were subjected to the screening by two virulent and one hypovirulent strains of Cryphonectria parasitica in order to examine reliability of ex situ tests for grading of blight resistance in chestnut. Branches 2-3 cm in diameter were harvested from the trees, in early April and end of September and stored at about 10°C for either 2 weeks (for branch sections) or 4 weeks (for bark- and wood-tissue sections). Branch sections and bark- and wood-tissue sections were inoculated with the C. parasitica strains and incubated in the dark at 25°C with 95% humidity for 4 weeks and/or 4 days, respectively. In branch sections, superficial cankers were very rare so only occurrence of pycnidia formed around the inoculating holes were evaluated. Pycnidia occurred more frequently in branch sections collected from the less resistant accessions in October trial. The size of necrotic areas reflected the resistance ranking of accessions in April/May better than in October inoculation trials. Results of inoculations on excised wood tissue were more consistent with the real blight resistance level than data obtained from inoculations on excised bark tissue. Occasional inconsistence between results of inoculations carried out at different dates or in different trees might be caused by different physiological state of collected branches. Based on these results it can be concluded that the end of dormant period seems to be the most suitable time to carry out both pathogenicity and resistance tests in chestnuts. |
Adamčíková, K; Pažitný, J; Pastirčáková, K Individual resistance of Fraxinus angustifolia and F. excelsior clones to Hymenoscyphus fraxineus Journal Article Journal of Plant Protection Research, 58 (3), pp. 227-233, 2018, ISSN: 1427-4345. @article{Adamčíková2018, title = {Individual resistance of \textit{Fraxinus angustifolia} and \textit{F. excelsior} clones to \textit{Hymenoscyphus fraxineus}}, author = {K. Adamčíková and J. Pažitný and K. Pastirčáková}, doi = {10.24425/122937}, issn = {1427-4345}, year = {2018}, date = {2018-10-22}, journal = {Journal of Plant Protection Research}, volume = {58}, number = {3}, pages = {227-233}, abstract = {Ash dieback, caused by Hymenoscyphus fraxineus, is a serious disease of common and narrow-leaved ash in Europe. The resistance of individual trees seems to be important for the maintenance of ash in European forests. In this in situ wound inoculation study, the susceptibility and differences in resistance to H. fraxineus between Fraxinus excelsior and F. angustifolia clones were assessed. Neither of the tested clones revealed total resistance to ash dieback; variety between the tested clones was observed. Differences in necroses lengths were significant between clones and between two ash species. Longer necroses were formed in F. angustifolia than in F. excelsior. Some clones exhibiting some resistance to the pathogen were identified. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Ash dieback, caused by Hymenoscyphus fraxineus, is a serious disease of common and narrow-leaved ash in Europe. The resistance of individual trees seems to be important for the maintenance of ash in European forests. In this in situ wound inoculation study, the susceptibility and differences in resistance to H. fraxineus between Fraxinus excelsior and F. angustifolia clones were assessed. Neither of the tested clones revealed total resistance to ash dieback; variety between the tested clones was observed. Differences in necroses lengths were significant between clones and between two ash species. Longer necroses were formed in F. angustifolia than in F. excelsior. Some clones exhibiting some resistance to the pathogen were identified. |
Camacho-Tapia, M; Sánchez-Soto, V; Correia, K C; Pastirčáková, K; Tovar-Pedraza, J M Powdery mildew of California poppy caused by Erysiphe eschscholziae in Mexico Journal Article Canadian Journal of Plant Pathology, 40 (3), pp. 461-466, 2018, ISSN: 0706-0661 . @article{Camacho-Tapia2018, title = {Powdery mildew of California poppy caused by \textit{Erysiphe eschscholziae} in Mexico}, author = {M. Camacho-Tapia and V. Sánchez-Soto and K.C. Correia and K. Pastirčáková and J.M. Tovar-Pedraza}, doi = {10.1080/07060661.2018.1498807}, issn = {0706-0661 }, year = {2018}, date = {2018-10-02}, journal = {Canadian Journal of Plant Pathology}, volume = {40}, number = {3}, pages = {461-466}, abstract = {Severe infection by powdery mildew was observed on California poppy (Eschscholzia californica) plants in Texcoco, State of Mexico, during spring of 2014 to 2017. Symptoms included colonies of white to greyish mildew-like growth on the abaxial and adaxial surfaces of the leaves. The identification of the fungal species was performed by examination of morphological structures using light microscopy and scanning electronmicroscope (SEM), as well as sequence analysis of the 5ʹ-end of 28S rRNA gene and the internal transcribed spacer (ITS) region of rDNA. Using the combination of morphological characterization and a phylogenetic analysis using Bayesian inference, the fungal agent was identified as Erysiphe eschscholziae. Pathogenicity tests were conducted on leaves of California poppy plants, and Koch’s postulates were fulfilled. This is the first report of E. eschscholziae causing powdery mildew on Eschscholzia californica in Mexico.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Severe infection by powdery mildew was observed on California poppy (Eschscholzia californica) plants in Texcoco, State of Mexico, during spring of 2014 to 2017. Symptoms included colonies of white to greyish mildew-like growth on the abaxial and adaxial surfaces of the leaves. The identification of the fungal species was performed by examination of morphological structures using light microscopy and scanning electronmicroscope (SEM), as well as sequence analysis of the 5ʹ-end of 28S rRNA gene and the internal transcribed spacer (ITS) region of rDNA. Using the combination of morphological characterization and a phylogenetic analysis using Bayesian inference, the fungal agent was identified as Erysiphe eschscholziae. Pathogenicity tests were conducted on leaves of California poppy plants, and Koch’s postulates were fulfilled. This is the first report of E. eschscholziae causing powdery mildew on Eschscholzia californica in Mexico. |
Pastirčáková, K; Adamčíková, K; Pastirčák, M; Zach, P; Galko, J; Kováč, M; Laco, J Two blue-stain fungi colonizing Scots pine (Pinus sylvestris) trees infested by bark beetles in Slovakia, Central Europe Journal Article Biologia, 73 (11), pp. 1053-1066, 2018, ISSN: 0006-3088. @article{Pastirčáková2018c, title = {Two blue-stain fungi colonizing Scots pine (\textit{Pinus sylvestris}) trees infested by bark beetles in Slovakia, Central Europe}, author = {K. Pastirčáková and K. Adamčíková and M. Pastirčák and P. Zach and J. Galko and M. Kováč and J. Laco}, doi = {10.2478/s11756-018-0114-6}, issn = {0006-3088}, year = {2018}, date = {2018-09-17}, journal = {Biologia}, volume = {73}, number = {11}, pages = {1053-1066}, abstract = {Excessive mortality of Scots pine (Pinus sylvestris) trees has recently been observed in the Záhorská nížina lowland in western Slovakia, Central Europe. Continuous drought, bark beetles and blue-stain fungi are likely to play a crucial role in this dieback of pines in a warm sandy area. Two blue-stain fungi, Ophiostoma ips and Ophiostoma minus, were found here on Scots pine trunks infested by bark beetles. These fungi were isolated and identified based on morphological properties and DNA sequences (ITS, LSU, β-tubulin). They colonized the inner bark, phloem and blue-stained sapwood, and were recorded in the galleries of Orthotomicus longicollis and other species of this genus. The occurrence of O. minus was massive; the fungus was found to produce perithecia abundantly in dying pines. Detailed descriptions, illustrations and global distribution of the two fungal species on host pine species are provided. The records of O. ips and those of O. minus are new for the mycoflora of Slovakia. Undetermined mycophagous nematodes were found inside the perithecia of O. ips. The role of bark beetles as real and potential vectors (Ips spp., Orthotomicus spp., Tomicus spp., Hylurgus ligniperda, Hylastes sp.) of blue-stain fungi in a declining pine forest is discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Excessive mortality of Scots pine (Pinus sylvestris) trees has recently been observed in the Záhorská nížina lowland in western Slovakia, Central Europe. Continuous drought, bark beetles and blue-stain fungi are likely to play a crucial role in this dieback of pines in a warm sandy area. Two blue-stain fungi, Ophiostoma ips and Ophiostoma minus, were found here on Scots pine trunks infested by bark beetles. These fungi were isolated and identified based on morphological properties and DNA sequences (ITS, LSU, β-tubulin). They colonized the inner bark, phloem and blue-stained sapwood, and were recorded in the galleries of Orthotomicus longicollis and other species of this genus. The occurrence of O. minus was massive; the fungus was found to produce perithecia abundantly in dying pines. Detailed descriptions, illustrations and global distribution of the two fungal species on host pine species are provided. The records of O. ips and those of O. minus are new for the mycoflora of Slovakia. Undetermined mycophagous nematodes were found inside the perithecia of O. ips. The role of bark beetles as real and potential vectors (Ips spp., Orthotomicus spp., Tomicus spp., Hylurgus ligniperda, Hylastes sp.) of blue-stain fungi in a declining pine forest is discussed. |
Desprez-Loustau, M L; Massot, M; Toïgo, M; Fort, T; Aday_Kaya, A G; Boberg, J; Braun, U; Capdevielle, X; Cech, T; Chandelier, A; Christova, P; Corcobado, T; Dogmus, T; Dutech, C; Fabreguettes, O; Faivre_d'Arcier, J; Gross, A; Horta_Jung, M; Iturrixa, E; Jung, T; Junker, C; Kiss, L; Kostov, K; Lehtijarvi, A; Lyubenova, A; Marçais, B; Oliva, J; Oskay, F; Pastirčák, M; Pastirčáková, K; Piou, D; Saint-Jean, G; Sallafranque, A; Slavov, S; Stenlid, J; Talgø, V; Takamatsu, T; Tack, A J M From leaf to continent: The multi-scale distribution of an invasive cryptic pathogen complex on oak Journal Article Fungal Ecology, 36 , pp. 39-50, 2018, ISSN: 1754-5048. @article{Desprez-Loustau2018, title = {From leaf to continent: The multi-scale distribution of an invasive cryptic pathogen complex on oak}, author = {M.L. Desprez-Loustau and M. Massot and M. Toïgo and T. Fort and A.G. Aday_Kaya and J. Boberg and U. Braun and X. Capdevielle and T. Cech and A. Chandelier and P. Christova and T. Corcobado and T. Dogmus and C. Dutech and O. Fabreguettes and J. Faivre_d'Arcier and A. Gross and M. Horta_Jung and E. Iturrixa and T. Jung and C. Junker and L. Kiss and K. Kostov and A. Lehtijarvi and A. Lyubenova and B. Marçais and J. Oliva and F. Oskay and M. Pastirčák and K. Pastirčáková and D. Piou and G. Saint-Jean and A. Sallafranque and S. Slavov and J. Stenlid and V. Talgø and T. Takamatsu and A.J.M. Tack}, doi = {10.1016/j.funeco.2018.08.001}, issn = {1754-5048}, year = {2018}, date = {2018-09-14}, journal = {Fungal Ecology}, volume = {36}, pages = {39-50}, abstract = {The spatial distribution and niche differentiation of three closely related species (Erysiphe alphitoides, Erysiphe quercicola and Erysiphe hypophylla) causing oak powdery mildew was studied at scales ranging from the European continent, where they are invasive, to a single leaf. While E. alphitoides was dominant at all scales, E. quercicola and E. hypophylla had restricted geographic, stand and leaf distributions. The large-scale distributions were likely explained by climatic factors and species environmental tolerances, with E. quercicola being more frequent in warmer climates and E. hypophylla in colder climates. The extensive sampling and molecular analyses revealed the cryptic invasion of E. quercicola in nine countries from which it had not previously been recorded. The presence of the three species was also strongly affected by host factors, such as oak species and developmental stage. Segregation patterns between Erysiphe species were observed at the leaf scale, between and within leaf surfaces, suggesting competitive effects.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The spatial distribution and niche differentiation of three closely related species (Erysiphe alphitoides, Erysiphe quercicola and Erysiphe hypophylla) causing oak powdery mildew was studied at scales ranging from the European continent, where they are invasive, to a single leaf. While E. alphitoides was dominant at all scales, E. quercicola and E. hypophylla had restricted geographic, stand and leaf distributions. The large-scale distributions were likely explained by climatic factors and species environmental tolerances, with E. quercicola being more frequent in warmer climates and E. hypophylla in colder climates. The extensive sampling and molecular analyses revealed the cryptic invasion of E. quercicola in nine countries from which it had not previously been recorded. The presence of the three species was also strongly affected by host factors, such as oak species and developmental stage. Segregation patterns between Erysiphe species were observed at the leaf scale, between and within leaf surfaces, suggesting competitive effects. |
Pastirčáková, K; Ostrovský, R; Pastirčák, M Mycobiota in dead and damaged branches of silver birch in Slovakia Journal Article Baltic Forestry, 24 (1), pp. 17-23, 2018, ISSN: 1392-1355. @article{Pastirčáková2018b, title = {Mycobiota in dead and damaged branches of silver birch in Slovakia}, author = {K. Pastirčáková and R. Ostrovský and M. Pastirčák}, url = {https://www.balticforestry.mi.lt/bf/PDF_Articles/2018-24%5B1%5D/Baltic%20Forestry%202018.1_017-023.pdf}, issn = {1392-1355}, year = {2018}, date = {2018-08-23}, journal = {Baltic Forestry}, volume = {24}, number = {1}, pages = {17-23}, abstract = {The species composition of the mycobiota in dead and damaged branches of silver birch (Betula pendula) was studied. The study material collected at 44 localities in Slovakia during the period of 2009 to 2015 was examined. In total, 27 fungal taxa (11 Ascomycetes and 16 Deuteromycetes) were identified on the basis of fruiting body morphology. Sixteen of them have never been recorded on birch trees in the country. Prosthemium betulinum, Trimmatostroma betulinum, Cytospora betulicola, Cryptosporella betulae, Coryneum lanciforme, Myxocyclus polycystis, Pleomassaria siparia, and Disculina betulina were the dominant colonisers of dead branches. The spectrum of fungi colonising dead and dying branches of B. pendula was compared in the following different types of stands: public parks and inter-block spaces of greenery, private gardens, forests, and tree alleys alongside roads. The average number of fungal taxa on birch trees growing in different habitats was not significantly different.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The species composition of the mycobiota in dead and damaged branches of silver birch (Betula pendula) was studied. The study material collected at 44 localities in Slovakia during the period of 2009 to 2015 was examined. In total, 27 fungal taxa (11 Ascomycetes and 16 Deuteromycetes) were identified on the basis of fruiting body morphology. Sixteen of them have never been recorded on birch trees in the country. Prosthemium betulinum, Trimmatostroma betulinum, Cytospora betulicola, Cryptosporella betulae, Coryneum lanciforme, Myxocyclus polycystis, Pleomassaria siparia, and Disculina betulina were the dominant colonisers of dead branches. The spectrum of fungi colonising dead and dying branches of B. pendula was compared in the following different types of stands: public parks and inter-block spaces of greenery, private gardens, forests, and tree alleys alongside roads. The average number of fungal taxa on birch trees growing in different habitats was not significantly different. |
Pažitný, J; Bolvanský, M; Adamčíková, K Screening for resistance of progenies derived from Castanea sativa × C. crenata and C. crenata to Cryphonectria parasitica Journal Article Forest Pathology, 48 (5), pp. e12439, 2018, ISSN: 1439-0329. @article{Pažitný2018, title = {Screening for resistance of progenies derived from Castanea sativa × C. crenata and C. crenata to Cryphonectria parasitica}, author = {J. Pažitný and M. Bolvanský and K. Adamčíková}, url = {https://onlinelibrary.wiley.com/doi/10.1111/efp.12439}, doi = {10.1111/efp.12439}, issn = {1439-0329}, year = {2018}, date = {2018-08-10}, journal = {Forest Pathology}, volume = {48}, number = {5}, pages = {e12439}, abstract = {The possibility of screening young seedlings of Castanea for chestnut blight resistance was examined to try to shorten time needed for a breeding programme. Two-year-old chestnut seedlings of eight progenies, derived from open and controlled pollination of three hybrids (Castanea sativa × C. crenata and a C. crenata tree by paternal tree of C. sativa) were screened for resistance to chestnut blight. One hybrid and a C. crenata were partially susceptible, and two hybrids were partially resistant to chestnut blight. A total of 179 seedlings were inoculated with two virulent and one hypovirulent strain of Cryphonectria parasitica at two dates (early June and early September). Stromata production was higher and canker size larger when seedlings were inoculated in early June. Differences in canker size among seedlings derived from different parental trees were observed only after September inoculation. However, differences in canker size among seedlings inoculated with the virulent strains were observed only after June inoculation. The only significant effect on seedling survival was based on fungal strains used for inoculation. Ratio of seedlings, which died after inoculation with the hypovirulent strain, was significantly lower (36.8%) than those that died after inoculation with virulent strains (88.4 and 95.1%). Survival of seedlings was affected only by fungal strains used in inoculation, and not influenced by parental trees. The risk of seedling dieback increased with increasing canker size and with decreasing stem diameter of seedlings. }, keywords = {}, pubstate = {published}, tppubtype = {article} } The possibility of screening young seedlings of Castanea for chestnut blight resistance was examined to try to shorten time needed for a breeding programme. Two-year-old chestnut seedlings of eight progenies, derived from open and controlled pollination of three hybrids (Castanea sativa × C. crenata and a C. crenata tree by paternal tree of C. sativa) were screened for resistance to chestnut blight. One hybrid and a C. crenata were partially susceptible, and two hybrids were partially resistant to chestnut blight. A total of 179 seedlings were inoculated with two virulent and one hypovirulent strain of Cryphonectria parasitica at two dates (early June and early September). Stromata production was higher and canker size larger when seedlings were inoculated in early June. Differences in canker size among seedlings derived from different parental trees were observed only after September inoculation. However, differences in canker size among seedlings inoculated with the virulent strains were observed only after June inoculation. The only significant effect on seedling survival was based on fungal strains used for inoculation. Ratio of seedlings, which died after inoculation with the hypovirulent strain, was significantly lower (36.8%) than those that died after inoculation with virulent strains (88.4 and 95.1%). Survival of seedlings was affected only by fungal strains used in inoculation, and not influenced by parental trees. The risk of seedling dieback increased with increasing canker size and with decreasing stem diameter of seedlings. |
Crous, P W; Wingfield, M J; Burgess, T I; Hardy, G.E.St.J.; Gené, J; Guarro, J; García, Baseia I G D; Gusmão, L F P; Souza-Motta, C M; Thangavel, R; Adamčík, S; Barili, A; Barnes, C W; Bezerra, J D P; Bordallo, J J; Cano-Lira, J F; de Oliveira, R J V; Ercole, E; Hubka, V; Iturrieta-González, I; Kubátová, A; Martín, M P; Moreau, P -A; Morte, A; Ordoñez, M E; Rodríguez, A; Stchigel, A M; Vizzini, A; Abdollahzadeh, J; Abreu, V P; Adamčíková, K; Albuquerque, G M R; Alexandrova, A V; Álvarez_Duarte, E; Armstrong-Cho, C; Banniza, S; Barbosa, R N; Bellanger, J -M; Bezerra, J L; Cabral, T S; Caicedo, Caboňand M E; Cantillo, T; Carnegie, A J; Carmo, L T; Castañeda-Ruiz, R F; Clement, C R; Čmoková, A; Conceição, L B; Cruz, R H S F; Damm, U; da_Silva, B D B; da_Silva, G A; da_Silva, R M F; Santiago, A.L.C.M._de_A.; de_Oliveira, L F; de_Souza, C A F; Déniel, F; Dima, B; Dong, G; Edwards, J; Félix, C R; Fournier, J; Gibertoni, T B; Hosaka, K; Iturriaga, T; Jadan, M; Jany, J -L; Jurjević, Ž; Kolařík, M; Kušan, I; Landell, M F; Leite_Cordeiro, T R; Lima, X D; Loizides, M; Luo, S; Machado, A R; Madrid, H; Magalhães, O M C; Marinho, P; Matočec, N; Mešić, A; Miller, A N; Morozova, O V; Neves, R P; Nonaka, K; Nováková, A; Oberlies, N H; Oliveira-Filho, J R C; Oliveira, T G L; Papp, V; Pereira, O L; Perrone, G; Peterson, S W; Pham, T H G; Raja, H A; Raudabaugh, D B; Řehulka, J; RodrÃguez-Andrade, E; Saba, M; Schauflerova, A; Shivas, R G; Simonini, G; Siqueira, J P Z; Sousa, J O; Stajsic, V; Svetasheva, T; Tan, Y P; Tkalčec, Z; Ullah, S; Valente, P; Valenzuela-Lopez, N; Abrinbana, M; Viana_Marques, D A; Wong, P T W; Xavier_de_Lima, V; Groenewald, J Z Fungal Planet description sheets: 716-784 Journal Article Persoonia, 40 , pp. 240-393, 2018, ISSN: 1878-9080. @article{Crous2018, title = {Fungal Planet description sheets: 716-784}, author = {P.W. Crous and M.J. Wingfield and T.I. Burgess and G.E.St.J. Hardy and J. Gené and J. Guarro and I.G. Baseia D. García and L.F.P. Gusmão and C.M. Souza-Motta and R. Thangavel and S. Adamčík and A. Barili and C.W. Barnes and J.D.P. Bezerra and J.J. Bordallo and J.F. Cano-Lira and R.J.V. de Oliveira and E. Ercole and V. Hubka and I. Iturrieta-González and A. Kubátová and M.P. Martín and P.-A. Moreau and A. Morte and M.E. Ordoñez and A. Rodríguez and A.M. Stchigel and A. Vizzini and J. Abdollahzadeh and V.P. Abreu and K. Adamčíková and G.M.R. Albuquerque and A.V. Alexandrova and E. Álvarez_Duarte and C. Armstrong-Cho and S. Banniza and R.N. Barbosa and J.-M. Bellanger and J.L. Bezerra and T.S. Cabral and M. Caboňand E. Caicedo and T. Cantillo and A.J. Carnegie and L.T. Carmo and R.F. Castañeda-Ruiz and C.R. Clement and A. Čmoková and L.B. Conceição and R.H.S.F Cruz and U. Damm and B.D.B. da_Silva and G.A. da_Silva and R.M.F. da_Silva and A.L.C.M._de_A. Santiago and L.F. de_Oliveira and C.A.F. de_Souza and F. Déniel and B. Dima and G. Dong and J. Edwards and C.R. Félix and J. Fournier and T.B. Gibertoni and K. Hosaka and T. Iturriaga and M. Jadan and J.-L. Jany and Ž. Jurjević and M. Kolařík and I. Kušan and M.F. Landell and T.R. Leite_Cordeiro and X.D. Lima and M. Loizides and S. Luo and A.R. Machado and H. Madrid and O.M.C. Magalhães and P. Marinho and N. Matočec and A. Mešić and A.N. Miller and O.V. Morozova and R.P. Neves and K. Nonaka and A. Nováková and N.H. Oberlies and J.R.C. Oliveira-Filho and T.G.L. Oliveira and V. Papp and O.L. Pereira and G. Perrone and S.W. Peterson and T.H.G. Pham and H.A. Raja and D.B. Raudabaugh and J. Řehulka and E. RodrÃguez-Andrade and M. Saba and A. Schauflerova and R.G. Shivas and G. Simonini and J.P.Z. Siqueira and J.O. Sousa and V. Stajsic and T. Svetasheva and Y.P. Tan and Z. Tkalčec and S. Ullah and P. Valente and N. Valenzuela-Lopez and M. Abrinbana and D.A. Viana_Marques and P.T.W. Wong and V. Xavier_de_Lima and J.Z. Groenewald}, doi = {https://doi.org/10.3767/persoonia.2018.40.10}, issn = {1878-9080}, year = {2018}, date = {2018-07-13}, journal = {Persoonia}, volume = {40}, pages = {240-393}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Adamčík, S; Dima, B; Adamčíková, K; Læssøe, T; Moreau, P A; Vizzini, A; Jančovičová, S European Hodophilus (Clavariaceae, Agaricales) species with yellow stipe Journal Article Mycological Progress, 17 (9), pp. 1097-1111, 2018, ISSN: ISSN: 1861-8952 . @article{Adamčík2018, title = {European Hodophilus (Clavariaceae, Agaricales) species with yellow stipe }, author = {S. Adamčík and B. Dima and K. Adamčíková and T. Læssøe and P.A. Moreau and A. Vizzini and S. Jančovičová}, doi = {https://doi.org/10.1007/s11557-018-1418-1}, issn = {ISSN: 1861-8952 }, year = {2018}, date = {2018-06-29}, journal = {Mycological Progress}, volume = {17}, number = {9}, pages = {1097-1111}, abstract = {Phylogenetic reconstruction of Hodophilus species with a yellow colour on the stipe based on nrITS, nrLSU and rpb2 sequences revealed six European species. All these species correspond to the widely accepted European concept of a single species Hodophilus micaceus. Four of these species are described and illustrated. H. micaceus and H. phaeoxanthus are recognised as two separate species and H. albofloccipes as a synonym of the latter. Two species, H. anatinus and H. cambriensis, are described as new. Possible endemism of H. micaceus and H. cambriensis to the British Isles is discussed. All analysed North American samples represent different species to those found in Europe. The North American species Hygrophorus rugulosus is combined in the genus Hodophilus. The preliminary key uses position and development of the yellow colour during maturation as themost important distinguishing character.The presence of the yellow colour is discussed as a possible synapomorphic character.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Phylogenetic reconstruction of Hodophilus species with a yellow colour on the stipe based on nrITS, nrLSU and rpb2 sequences revealed six European species. All these species correspond to the widely accepted European concept of a single species Hodophilus micaceus. Four of these species are described and illustrated. H. micaceus and H. phaeoxanthus are recognised as two separate species and H. albofloccipes as a synonym of the latter. Two species, H. anatinus and H. cambriensis, are described as new. Possible endemism of H. micaceus and H. cambriensis to the British Isles is discussed. All analysed North American samples represent different species to those found in Europe. The North American species Hygrophorus rugulosus is combined in the genus Hodophilus. The preliminary key uses position and development of the yellow colour during maturation as themost important distinguishing character.The presence of the yellow colour is discussed as a possible synapomorphic character. |
Barta, M; Kautmanová, I; Čičková, H; Ferenčík, J; Florián, Š; Novotný, J; Kozánek, M The potential of Beauveria bassiana inoculum formulated into a polymeric matrix for a microbial control of spruce bark beetle Journal Article Biocontrol Science and Technology, 28 (7), pp. 718-735, 2018, ISSN: 1360-0478. @article{Barta2018b, title = {The potential of Beauveria bassiana inoculum formulated into a polymeric matrix for a microbial control of spruce bark beetle}, author = {M. Barta and I. Kautmanová and H. Čičková and J. Ferenčík and Š. Florián and J. Novotný and M. Kozánek}, url = {https://doi.org/10.1080/09583157.2018.1487027}, doi = {10.1080/09583157.2018.1487027}, issn = {1360-0478}, year = {2018}, date = {2018-06-13}, journal = {Biocontrol Science and Technology}, volume = {28}, number = {7}, pages = {718-735}, abstract = {Two local strains of Beauveria bassiana originally isolated from naturally infected spruce bark beetles in Slovakia were tested for their virulence to Ips typographus (IT) and for their compatibility with a polymeric matrix composed of low-molecular polyethylene. Conidia could be homogenously immobilized in the low-molecular polyethylene matrix with no adverse effect on their viability and infectivity. At constant temperature (25°C), viability of immobilized conidial decreased only by 1–2% after 7 or 14 days when compared with non-formulated conidia. In field conditions, viability of conidia formulated in the matrix was even significantly higher than non-formulated conidia 35 days after their application in traps. Conidia incorporated into the polymeric matrix were infective to IT adults in laboratory bioassays. Mean values of LC50 for native conidia (0.72–2.05 × 106 conidia ml−1) and conidia immobilized in the polymeric matrix (0.64–1.03 × 105 conidia mm−2) demonstrated high virulence. The efficacy of the local strains was significantly higher than that of B. bassiana strains from mycoinsecticides (Boverol®, Botanigard® ES and Naturalis-L®). Results showed potential of this polymeric material for its use in microbial control of IT when mixed with conidia of B. bassiana.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two local strains of Beauveria bassiana originally isolated from naturally infected spruce bark beetles in Slovakia were tested for their virulence to Ips typographus (IT) and for their compatibility with a polymeric matrix composed of low-molecular polyethylene. Conidia could be homogenously immobilized in the low-molecular polyethylene matrix with no adverse effect on their viability and infectivity. At constant temperature (25°C), viability of immobilized conidial decreased only by 1–2% after 7 or 14 days when compared with non-formulated conidia. In field conditions, viability of conidia formulated in the matrix was even significantly higher than non-formulated conidia 35 days after their application in traps. Conidia incorporated into the polymeric matrix were infective to IT adults in laboratory bioassays. Mean values of LC50 for native conidia (0.72–2.05 × 106 conidia ml−1) and conidia immobilized in the polymeric matrix (0.64–1.03 × 105 conidia mm−2) demonstrated high virulence. The efficacy of the local strains was significantly higher than that of B. bassiana strains from mycoinsecticides (Boverol®, Botanigard® ES and Naturalis-L®). Results showed potential of this polymeric material for its use in microbial control of IT when mixed with conidia of B. bassiana. |
Ondrušková, E; Jánošíková, Z; Adamčík, S; Kádasi-Horáková, M; Rákusová-Sládková, D; Adamčíková, K Needle blight caused by Dothistroma pini in Slovakia: distribution, host range and mating types Journal Article Scandinavian Journal of Forest Research, 33 (7), pp. 650-656, 2018, ISSN: 1651-1891. @article{Ondrušková2018, title = {Needle blight caused by Dothistroma pini in Slovakia: distribution, host range and mating types}, author = {E. Ondrušková and Z. Jánošíková and S. Adamčík and M. Kádasi-Horáková and D. Rákusová-Sládková and K. Adamčíková}, doi = {10.1080/02827581.2018.1482954}, issn = {1651-1891}, year = {2018}, date = {2018-06-06}, journal = {Scandinavian Journal of Forest Research}, volume = {33}, number = {7}, pages = {650-656}, abstract = {Dothistroma needle blight (DNB) has been observed in Slovakia during the last two decades. Up until 2017, Dothistroma septosporum has only been detected and molecularly confirmed to cause DNB in Slovakia. Here, we report the detection of Dothistroma pini at six localities around Slovakia, representing different plantation types. Four pine species (Pinus sylvestris, P. nigra, P. mugo and P. jeffreyi) were confirmed as hosts of D. pini in Slovakia, of which only P. mugo has been previously reported as host in Slovakia. Three gene regions (ITS, EF1 –α, and ß-tubulin) of each of the 13 isolates were sequenced and assigned as D. pini. Based on ITS sequences, the studied isolates represent the haplotypes Dp_HAP.1, Dp_HAP.2. Both mating types were detected but at different localities. Our results suggest that in addition to D. septosporum, D. pini may contribute to DNB also in Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) has been observed in Slovakia during the last two decades. Up until 2017, Dothistroma septosporum has only been detected and molecularly confirmed to cause DNB in Slovakia. Here, we report the detection of Dothistroma pini at six localities around Slovakia, representing different plantation types. Four pine species (Pinus sylvestris, P. nigra, P. mugo and P. jeffreyi) were confirmed as hosts of D. pini in Slovakia, of which only P. mugo has been previously reported as host in Slovakia. Three gene regions (ITS, EF1 –α, and ß-tubulin) of each of the 13 isolates were sequenced and assigned as D. pini. Based on ITS sequences, the studied isolates represent the haplotypes Dp_HAP.1, Dp_HAP.2. Both mating types were detected but at different localities. Our results suggest that in addition to D. septosporum, D. pini may contribute to DNB also in Slovakia. |
Pastirčáková, K; Ivanová, H; Pastirčák, M Species diversity of fungi on damaged branches and leaves of ashes (Fraxinus spp.) in different types of stands in Slovakia Journal Article Central European Forestry Journal, 64 (2), pp. 133-139, 2018, ISSN: 0323-1046. @article{Pastirčáková2018, title = {Species diversity of fungi on damaged branches and leaves of ashes (\textit{Fraxinus} spp.) in different types of stands in Slovakia}, author = {K. Pastirčáková and H. Ivanová and M. Pastirčák}, url = {https://content.sciendo.com/view/journals/forj/64/2/article-p133.xml}, doi = {10.1515/forj-2017-0035}, issn = {0323-1046}, year = {2018}, date = {2018-04-16}, journal = {Central European Forestry Journal}, volume = {64}, number = {2}, pages = {133-139}, abstract = {The diversity of fungi on branches and leaves of ashes (Fraxinus angustifolia, F. excelsior, F. ornus) in Slovakia was studied. Symptomatic material collected in Slovakia during the period of 2013 to 2017 and herbarium specimens previously collected were examined. In total, 30 fungal taxa (15 Deuteromycetes, 14 Ascomycetes and one Basidiomycetes) were recorded. Twenty-three of them have never been recorded on ashes in the country. The most frequently occurring fungi were Hymenoscyphus fraxineus (anamorph Chalara fraxinea) that causes necrosis of shoots and branches, and Phyllactinia fraxini, a foliar pathogen that causes powdery mildew disease. Fungal diversity on ashes growing in different types of stands was compared. Species richness was the greatest in seed orchards (20 fungal taxa) compared to private gardens, which contained the lowest (two fungal taxa). Species diversity in forest stands comprised 18 fungal taxa and the urban greenery was represented by 10 fungal taxa. Nine fungal taxa were recorded in tree alley along the road. The widest fungal species spectrum was recorded on F. excelsior.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The diversity of fungi on branches and leaves of ashes (Fraxinus angustifolia, F. excelsior, F. ornus) in Slovakia was studied. Symptomatic material collected in Slovakia during the period of 2013 to 2017 and herbarium specimens previously collected were examined. In total, 30 fungal taxa (15 Deuteromycetes, 14 Ascomycetes and one Basidiomycetes) were recorded. Twenty-three of them have never been recorded on ashes in the country. The most frequently occurring fungi were Hymenoscyphus fraxineus (anamorph Chalara fraxinea) that causes necrosis of shoots and branches, and Phyllactinia fraxini, a foliar pathogen that causes powdery mildew disease. Fungal diversity on ashes growing in different types of stands was compared. Species richness was the greatest in seed orchards (20 fungal taxa) compared to private gardens, which contained the lowest (two fungal taxa). Species diversity in forest stands comprised 18 fungal taxa and the urban greenery was represented by 10 fungal taxa. Nine fungal taxa were recorded in tree alley along the road. The widest fungal species spectrum was recorded on F. excelsior. |
Jánošíková-Hečková, Z; Ondrušková, E; Barta, M; Ostrovský, R; Kádasi-Horáková, M; Pastirčáková, K; Kobza, M; Adamčíková, K The hosts and geographic range of Dothistroma needle blight in Slovakia Journal Article Forest Pathology, 48 (3), pp. e12421, 2018, ISSN: 1437-4781. @article{Jánošíková-Hečková2018, title = {The hosts and geographic range of Dothistroma needle blight in Slovakia}, author = {Z. Jánošíková-Hečková and E. Ondrušková and M. Barta and R. Ostrovský and M. Kádasi-Horáková and K. Pastirčáková and M. Kobza and K. Adamčíková }, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/efp.12421}, doi = {10.1111/efp.12421}, issn = {1437-4781}, year = {2018}, date = {2018-02-15}, journal = {Forest Pathology}, volume = {48}, number = {3}, pages = {e12421}, abstract = {The occurrence and distribution of Dothistroma needle blight (DNB) were studied in 2014–2017 around Slovakia. A total of 84 localities, both native and planted, were investigated, and the presence of DNB was confirmed in 73 of them. In all positive locations, symptoms typical of DNB were observed and the Dothistroma species was confirmed using species-specific primers either from fungal cultures or directly from needles. Both Dothistroma species—D. septosporum and D. pini—were identified. Both species occurred together in 29 locations, only D. septosporum in 42 and only D. pini in two locations. The host range of D. septosporum included 10 pine species and two spruce species. The host range of D. pini comprised the same number of pine hosts but only one spruce species. Five pine hosts, P. aristata, P. coulteri, P. densiflora, P. jeffreyi, P. × schwerinii, and one spruce host P. abies are new hosts species of D. pini. P. densiflora and Picea pungens have earlier been reported to be susceptible for DNB. In this study, D. septosporum was found from both tree species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The occurrence and distribution of Dothistroma needle blight (DNB) were studied in 2014–2017 around Slovakia. A total of 84 localities, both native and planted, were investigated, and the presence of DNB was confirmed in 73 of them. In all positive locations, symptoms typical of DNB were observed and the Dothistroma species was confirmed using species-specific primers either from fungal cultures or directly from needles. Both Dothistroma species—D. septosporum and D. pini—were identified. Both species occurred together in 29 locations, only D. septosporum in 42 and only D. pini in two locations. The host range of D. septosporum included 10 pine species and two spruce species. The host range of D. pini comprised the same number of pine hosts but only one spruce species. Five pine hosts, P. aristata, P. coulteri, P. densiflora, P. jeffreyi, P. × schwerinii, and one spruce host P. abies are new hosts species of D. pini. P. densiflora and Picea pungens have earlier been reported to be susceptible for DNB. In this study, D. septosporum was found from both tree species. |
Barta, M In planta bioassay on the effects of endophytic Beauveria strains against larvae of horse-chestnut leaf miner (Cameraria ohridella) Journal Article Biological Control, 121 , pp. 88-98, 2018, ISSN: 1049-9644. @article{Barta2018c, title = {In planta bioassay on the effects of endophytic Beauveria strains against larvae of horse-chestnut leaf miner (Cameraria ohridella)}, author = {M. Barta}, url = {https://doi.org/10.1016/j.biocontrol.2018.02.013}, doi = {10.1016/j.biocontrol.2018.02.013}, issn = {1049-9644}, year = {2018}, date = {2018-02-14}, journal = {Biological Control}, volume = {121}, pages = {88-98}, abstract = {Cameraria ohridella is a major pest of horse-chestnut trees in Europe causing permanent outbreaks and severe defoliations. Entomopathogenic Beauveria species are common mortality factors of C. ohridella pupae overwintering in leaf litters. In this study, laboratory bioassays were conducted to determine the ability of local Beauveria strains to colonize horse-chestnut leaves endophytically and test their antagonistic activity against C. ohridella in colonized leaves. Two strains of Beauveria bassiana (AM_EF0111 isolated from horse-chestnut leaf tissue and AM_EP0715 isolated from a C. ohridella pupa) and one strain of B. pseudobassiana (AM_SO1015 obtained from soil) were used for in planta bioassays. The selected strains demonstrated high virulence to leaf miner pupae after a topical treatment with spore suspensions. LC50 ranged between 2.49 and 3.19 × 106 conidia ml−1. Horse-chestnut saplings were inoculated with the fungi through their direct immersion into spore suspensions (107 conidia ml−1). Endophytic colonization was successful and a mean colonization rate reached 57.44–66.08% 15 days post-inoculation. It decreased over time for all strains, but the fungi were still present in leaf tissue 54 days post-inoculation. AM_EF0111 strain was significantly more effective in colonizing saplings than AM_SO1015 and AM_EP0715. Fungus-colonized and untreated (control) saplings were infested with the leaf miner 31 days after inoculation and development of larval mines was monitored. In Beauveria-colonized saplings, damage by leaf miner was significantly lower compared to control plants. On day 23 post-infestation, the damaged leaf area of colonized leaves was 5-times smaller compared to control saplings. The negative effect on larval tunneling was significantly greater for strain AM_EP0715 than for the other two strains. Survival of leaf miners was considerably affected in colonized leaves, but mycosis was detected only in 5.41–9.23% of cadavers. No mycosed individuals were collected from control saplings. Pupae from colonized leaves were smaller in dimensions with lower weight than pupae form control plants. The same Beauveria strains recovered from leaves at the end of colonization bioassays showed no significant decrease in LC50 values.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cameraria ohridella is a major pest of horse-chestnut trees in Europe causing permanent outbreaks and severe defoliations. Entomopathogenic Beauveria species are common mortality factors of C. ohridella pupae overwintering in leaf litters. In this study, laboratory bioassays were conducted to determine the ability of local Beauveria strains to colonize horse-chestnut leaves endophytically and test their antagonistic activity against C. ohridella in colonized leaves. Two strains of Beauveria bassiana (AM_EF0111 isolated from horse-chestnut leaf tissue and AM_EP0715 isolated from a C. ohridella pupa) and one strain of B. pseudobassiana (AM_SO1015 obtained from soil) were used for in planta bioassays. The selected strains demonstrated high virulence to leaf miner pupae after a topical treatment with spore suspensions. LC50 ranged between 2.49 and 3.19 × 106 conidia ml−1. Horse-chestnut saplings were inoculated with the fungi through their direct immersion into spore suspensions (107 conidia ml−1). Endophytic colonization was successful and a mean colonization rate reached 57.44–66.08% 15 days post-inoculation. It decreased over time for all strains, but the fungi were still present in leaf tissue 54 days post-inoculation. AM_EF0111 strain was significantly more effective in colonizing saplings than AM_SO1015 and AM_EP0715. Fungus-colonized and untreated (control) saplings were infested with the leaf miner 31 days after inoculation and development of larval mines was monitored. In Beauveria-colonized saplings, damage by leaf miner was significantly lower compared to control plants. On day 23 post-infestation, the damaged leaf area of colonized leaves was 5-times smaller compared to control saplings. The negative effect on larval tunneling was significantly greater for strain AM_EP0715 than for the other two strains. Survival of leaf miners was considerably affected in colonized leaves, but mycosis was detected only in 5.41–9.23% of cadavers. No mycosed individuals were collected from control saplings. Pupae from colonized leaves were smaller in dimensions with lower weight than pupae form control plants. The same Beauveria strains recovered from leaves at the end of colonization bioassays showed no significant decrease in LC50 values. |
Barta, M; Kautmanová, I; Čičková, H; Ferenčík, J; Florián, Š; Novotný, J; Kozánek, M Biologia, 73 (1), pp. 53-65, 2018, ISSN: 1336-9563. @article{Barta2018, title = {Hypocrealean fungi associated with populations of Ips typographus in West Carpathians and selection of local Beauveria strains for effective bark beetle control}, author = {M. Barta and I. Kautmanová and H. Čičková and J. Ferenčík and Š. Florián and J. Novotný and M. Kozánek}, url = {https://doi.org/10.2478/s11756-018-0005-x}, doi = {10.2478/s11756-018-0005-x}, issn = {1336-9563}, year = {2018}, date = {2018-02-05}, journal = {Biologia}, volume = {73}, number = {1}, pages = {53-65}, abstract = {In Slovakia, a diversity of entomopathogenic fungi (Ascomycota, Hypocreales) associated with outbreaks of Ips typographus was studied in 81 localities and as many as 113 in vitro cultures of five entomopathogenic species were isolated from infected individuals: Beauveria bassiana (87 isolates), B. pseudobassiana (14 isolates), B. caledonica (6 isolates), Lecanicillium lecanii (4 isolates) and Isaria farinosa (2 isolates). B. pseudobassiana is recorded in natural populations of I. typographus for the first time. Biological properties of selected Beauveria isolates, including colony growth, biomass production, conidia yield and pathogenicity to I. typographus adults, were studied in a series of laboratory bioassays and much intra- and interspecific variability was detected. B. bassiana isolates produced biomass or conidia at significantly higher rate than B. pseudobassiana and B. caledonica isolates. Two B. bassiana isolates were selected as the most virulent to bark beetle adults, demonstrating a mean LC50 ranging from 0.72 to 2.05 × 106 conidia ml−1, and were qualified as promising candidates for biocontrol of I. typographus. Their virulence was significantly higher than that of the mycoinsecticides Boverol®, which was used as a reference strain in the virulence bioassays.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In Slovakia, a diversity of entomopathogenic fungi (Ascomycota, Hypocreales) associated with outbreaks of Ips typographus was studied in 81 localities and as many as 113 in vitro cultures of five entomopathogenic species were isolated from infected individuals: Beauveria bassiana (87 isolates), B. pseudobassiana (14 isolates), B. caledonica (6 isolates), Lecanicillium lecanii (4 isolates) and Isaria farinosa (2 isolates). B. pseudobassiana is recorded in natural populations of I. typographus for the first time. Biological properties of selected Beauveria isolates, including colony growth, biomass production, conidia yield and pathogenicity to I. typographus adults, were studied in a series of laboratory bioassays and much intra- and interspecific variability was detected. B. bassiana isolates produced biomass or conidia at significantly higher rate than B. pseudobassiana and B. caledonica isolates. Two B. bassiana isolates were selected as the most virulent to bark beetle adults, demonstrating a mean LC50 ranging from 0.72 to 2.05 × 106 conidia ml−1, and were qualified as promising candidates for biocontrol of I. typographus. Their virulence was significantly higher than that of the mycoinsecticides Boverol®, which was used as a reference strain in the virulence bioassays. |
Ivanová, H Identification and characterization of the fungus Dothiorella sarmentorum on necrotic shoots of declining ash in Slovakia Journal Article Folia Oecologica, 45 (1), pp. 53-57, 2018, ISSN: 1336-5266. @article{Helena2018, title = {Identification and characterization of the fungus Dothiorella sarmentorum on necrotic shoots of declining ash in Slovakia}, author = {H. Ivanová}, url = {http://ife.sk/wp-content/uploads/2016/10/foecol-2018-0006.pdf}, doi = {10.2478/foecol-2018-0006}, issn = {1336-5266}, year = {2018}, date = {2018-01-01}, journal = {Folia Oecologica}, volume = {45}, number = {1}, pages = {53-57}, abstract = {Formerly, before the current Hymenoscyphus fraxineus epidemic, symptomatic ash twigs were habitually colonized by fungi occurring occasionally in the early stages of ash diseases. Some of these fungi are endophytes or facultative parasites. The segments of diseased shoots of the studied trees were collected from a seed orchard situated in southwest Slovakia. The frequently isolated fungus Dothiorella sarmentorum was identified microscopically and characterized with the aid of morphological keys. The disease symptoms comprise wood cankers, bud necrosis, and shoot and branch dieback displayed through bleached, necrotic or discoloured canes in infected trees. Despite the Dothiorella species has not yet been recognized to have significant economic consequences, the cumulative effects of various stressful biotic and abiotic factors may induce disorder and decline of ash trees in Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Formerly, before the current Hymenoscyphus fraxineus epidemic, symptomatic ash twigs were habitually colonized by fungi occurring occasionally in the early stages of ash diseases. Some of these fungi are endophytes or facultative parasites. The segments of diseased shoots of the studied trees were collected from a seed orchard situated in southwest Slovakia. The frequently isolated fungus Dothiorella sarmentorum was identified microscopically and characterized with the aid of morphological keys. The disease symptoms comprise wood cankers, bud necrosis, and shoot and branch dieback displayed through bleached, necrotic or discoloured canes in infected trees. Despite the Dothiorella species has not yet been recognized to have significant economic consequences, the cumulative effects of various stressful biotic and abiotic factors may induce disorder and decline of ash trees in Slovakia. |
Ivanová, Helena; Onderková, Anna; Pristaš, Peter Sordaria fimicola-like ascomycete isolated from Pinus coulteri needles in Slovakia Journal Article Biologia, 73 (6), pp. 553-559, 2018, ISSN: ISSN 0006-3088. @article{Ivanová2018, title = {Sordaria fimicola-like ascomycete isolated from Pinus coulteri needles in Slovakia}, author = {Helena Ivanová and Anna Onderková and Peter Pristaš}, doi = {https://doi.org/10.2478/s11756-018-0071-0}, issn = {ISSN 0006-3088}, year = {2018}, date = {2018-00-00}, journal = {Biologia}, volume = {73}, number = {6}, pages = {553-559}, abstract = {This is the first report of Sordaria fimicola-like ascomycete which was encountered during a diversity study of injured tissues of coulter pine in Slovakia. The fungus was identified as Sordaria fimicola by morphological analyses. Sequence analysis of internal transcribed spacer region (ITS) showed that the fungus is highly related to the ITS sequences of several S. fimicola isolates documenting wide ecological valence and geographical distribution of S. fimicola-like ascomycetes}, keywords = {}, pubstate = {published}, tppubtype = {article} } This is the first report of Sordaria fimicola-like ascomycete which was encountered during a diversity study of injured tissues of coulter pine in Slovakia. The fungus was identified as Sordaria fimicola by morphological analyses. Sequence analysis of internal transcribed spacer region (ITS) showed that the fungus is highly related to the ITS sequences of several S. fimicola isolates documenting wide ecological valence and geographical distribution of S. fimicola-like ascomycetes |
2017 |
Pastirčák, M; Pastirčáková, K Hypoderma rubi on two new hosts in Slovakia Journal Article Mycotaxon, 132 (4), pp. 849–855, 2017, ISSN: 0093-4666. @article{Pastirčák30.1, title = {\textit{Hypoderma rubi} on two new hosts in Slovakia}, author = {M. Pastirčák and K. Pastirčáková}, doi = {10.5248/132.849}, issn = {0093-4666}, year = {2017}, date = {2017-12-30}, journal = {Mycotaxon}, volume = {132}, number = {4}, pages = {849–855}, abstract = {Hypoderma rubi was found on the previous year’s fallen petioles of Fraxinus chinensis subsp. rhynchophylla and on attached dead twigs of Rhododendron fortunei in Slovakia. The fungus, which is recorded for the first time on these host taxa, also represents a new taxon for the Slovak mycota. The morphological characteristics of the fungus found on Slovak collections are described.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Hypoderma rubi was found on the previous year’s fallen petioles of Fraxinus chinensis subsp. rhynchophylla and on attached dead twigs of Rhododendron fortunei in Slovakia. The fungus, which is recorded for the first time on these host taxa, also represents a new taxon for the Slovak mycota. The morphological characteristics of the fungus found on Slovak collections are described. |
Jančovičová, S; Adamčík, S; Looney, B P; Caboň, M; Čaplovičová, M; Kopáni, M; Pennycook, S R; Adamčíková, K Delimitation of European Crepidotus stenocystis as different from the North American species C. brunnescens (Crepidotaceae, Agariccales) Journal Article Phytotaxa, 328 (2), pp. 127-139, 2017, ISSN: 1179-3163. @article{Jančovičová2017, title = {Delimitation of European Crepidotus stenocystis as different from the North American species C. brunnescens (Crepidotaceae, Agariccales)}, author = {S. Jančovičová and S. Adamčík and B.P. Looney and M. Caboň and M. Čaplovičová and M. Kopáni and S.R. Pennycook and K. Adamčíková}, doi = {https://doi.org/10.11646/phytotaxa.328.2.3}, issn = {1179-3163}, year = {2017}, date = {2017-11-17}, journal = {Phytotaxa}, volume = {328}, number = {2}, pages = {127-139}, abstract = {Crepidotus stenocystis and C. brunnescens are morphologically similar species defined by globose to subglobose spores, the presence of clamp connections in all tissues and bottle-like or flask-like cheilocystidia. They are also similar in the pileal aspect which is hygrophanous, glabrous or white-fibrillose, at first white but becoming brownish with age. Each are described from separate continents and have only been reported from their respective continents, C. stenocystis from Europe and C. brunnescens from North America. The phylogenetic analysis of IT S and LSU nrDNA regions from original type material confirms the existence of two distinct species, C. brunnescens that is more closely related to C. malachioides, and C. stenocystis that is more closely related to C. applanatus. Crepidotus stenocystis differs from C. brunnescens by its more prominent spore ornamentation and longer cheilocystidia that are frequently narrowly utriform. Based on the studied material and published data, it seems that C. stenocystis is distributed throughout all of Europe and does not occur in North America, whereas C. brunnescens is only known from Michigan in the USA.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Crepidotus stenocystis and C. brunnescens are morphologically similar species defined by globose to subglobose spores, the presence of clamp connections in all tissues and bottle-like or flask-like cheilocystidia. They are also similar in the pileal aspect which is hygrophanous, glabrous or white-fibrillose, at first white but becoming brownish with age. Each are described from separate continents and have only been reported from their respective continents, C. stenocystis from Europe and C. brunnescens from North America. The phylogenetic analysis of IT S and LSU nrDNA regions from original type material confirms the existence of two distinct species, C. brunnescens that is more closely related to C. malachioides, and C. stenocystis that is more closely related to C. applanatus. Crepidotus stenocystis differs from C. brunnescens by its more prominent spore ornamentation and longer cheilocystidia that are frequently narrowly utriform. Based on the studied material and published data, it seems that C. stenocystis is distributed throughout all of Europe and does not occur in North America, whereas C. brunnescens is only known from Michigan in the USA. |
Adamčík, S; Jančovičová, S; Looney, B P; Adamčíková, K; Griffith, G W; Læssøe, T; Moreau, P A; Vizzini, A; Matheny, P B Hodophilus (Clavariaceae, Agaricales) species with dark dots on the stipe: more than one species in Europe Journal Article Mycological Progress, 16 (8), pp. 811–821, 2017, ISSN: 1861-8952 . @article{Adamčík2017b, title = {Hodophilus (Clavariaceae, Agaricales) species with dark dots on the stipe: more than one species in Europe}, author = {S. Adamčík and S. Jančovičová and B.P. Looney and K. Adamčíková and G. W. Griffith and T. Læssøe and P.A. Moreau and A. Vizzini and P. B. Matheny }, doi = {DOI 10.1007/s11557-017-1318-9}, issn = {1861-8952 }, year = {2017}, date = {2017-07-01}, journal = {Mycological Progress}, volume = {16}, number = {8}, pages = {811–821}, abstract = {Hodophilus atropunctus is traditionally defined as the only species of this genus with dark brown or black dots on the stipe. Multi-locus phylogenetic reconstruction recognised two distinct clades morphologically corresponding to this species concept. The limited morphological description in the protologue of H. atropunctus and absence of a type specimen were limitations in an assignment of this name to one of the recognised phylogenetic species. The emended species concept and the selection of a neotype are based on careful analyses of the colour of the basidiomata and how this changes during maturation and drying. The name H. atropunctus is assigned to the paler of the two species which also shows colour change across the pileus and along the length of the stipe when dry. The second darker species is described here as new, H. variabilipes, but only seven out of 14 collections examined belonging to this taxon had distinct dark coloured dots on the stipe surface.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Hodophilus atropunctus is traditionally defined as the only species of this genus with dark brown or black dots on the stipe. Multi-locus phylogenetic reconstruction recognised two distinct clades morphologically corresponding to this species concept. The limited morphological description in the protologue of H. atropunctus and absence of a type specimen were limitations in an assignment of this name to one of the recognised phylogenetic species. The emended species concept and the selection of a neotype are based on careful analyses of the colour of the basidiomata and how this changes during maturation and drying. The name H. atropunctus is assigned to the paler of the two species which also shows colour change across the pileus and along the length of the stipe when dry. The second darker species is described here as new, H. variabilipes, but only seven out of 14 collections examined belonging to this taxon had distinct dark coloured dots on the stipe surface. |
Montalva, C; Valenzuela, E; Barta, M; Rojas, E; Arismendi, N; Rodrigues, J; Humber, R A Lecanicillium attenuatum isolates affecting the invasive cypress aphid (Cinara cupressi) in Chile Journal Article BioControl, 62 (5), pp. 625–637, 2017, ISSN: 1386-6141. @article{Montalva2017, title = {\textit{Lecanicillium attenuatum} isolates affecting the invasive cypress aphid (\textit{Cinara cupressi}) in Chile}, author = {C. Montalva and E. Valenzuela and M. Barta and E. Rojas and N. Arismendi and J. Rodrigues and R.A. Humber}, url = {https://link.springer.com/article/10.1007/s10526-017-9817-9}, doi = {10.1007/s10526-017-9817-9}, issn = {1386-6141}, year = {2017}, date = {2017-05-16}, journal = {BioControl}, volume = {62}, number = {5}, pages = {625–637}, abstract = {The cypress aphid (Cinara cupressi) is listed among the hundred most important invasive pests in the world. In Chile, it was first detected in 2003 and currently is present throughout the country. In the course of a survey of their natural enemies in Chile, three strains of entomopathogenic fungi were isolated. The isolates were identified and tested against the aphid in laboratory experiments. Two further entomopathogenic fungi (ARSEF 5126 and 5128), formulated in the mycoinsecticides Vertalec and Mycotal, were used as reference strains. The three Chilean isolates were identified genomically as Lecanicillium attenuatum and were pathogenic to third-instar nymphs. The isolate ARSEF 13279 yielded the lowest overall lethal concentration (LC50), 3 x 105 conidia ml-1 at four days post-inoculation, and the shortest lethal time (LT50), 3.7 days after inoculation with 106 conidia ml-1. The results indicate that the isolates have considerable potential as microbial control agents of the invasive cypress aphid.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The cypress aphid (Cinara cupressi) is listed among the hundred most important invasive pests in the world. In Chile, it was first detected in 2003 and currently is present throughout the country. In the course of a survey of their natural enemies in Chile, three strains of entomopathogenic fungi were isolated. The isolates were identified and tested against the aphid in laboratory experiments. Two further entomopathogenic fungi (ARSEF 5126 and 5128), formulated in the mycoinsecticides Vertalec and Mycotal, were used as reference strains. The three Chilean isolates were identified genomically as Lecanicillium attenuatum and were pathogenic to third-instar nymphs. The isolate ARSEF 13279 yielded the lowest overall lethal concentration (LC50), 3 x 105 conidia ml-1 at four days post-inoculation, and the shortest lethal time (LT50), 3.7 days after inoculation with 106 conidia ml-1. The results indicate that the isolates have considerable potential as microbial control agents of the invasive cypress aphid. |
Kádasi-Horáková, M; Adamčíková, K; Pastirčáková, K; Longauerová, V; Maľová, M Natural infection of Fraxinus angustifolia by Hymenoscyphus fraxineus in Slovakia Journal Article Baltic Forestry, 23 (1), pp. 52-55, 2017, ISSN: 2029-9230. @article{M.2017b, title = {Natural infection of \textit{Fraxinus angustifolia} by \textit{Hymenoscyphus fraxineus} in Slovakia}, author = {M. Kádasi-Horáková and K. Adamčíková and K. Pastirčáková and V. Longauerová and M. Maľová}, url = {https://www.balticforestry.mi.lt/bf/PDF_Articles/2017-23%5B1%5D/Baltic%20Forestry%202017.1_052-055.pdf}, issn = {2029-9230}, year = {2017}, date = {2017-04-07}, journal = {Baltic Forestry}, volume = {23}, number = {1}, pages = {52-55}, abstract = {The fungus Hymenoscyphus fraxineus is responsible for dieback of common ash (Fraxinus excelsior) and in some parts of Europe also of narrow-leaved ash (F. angustifolia). The first symptoms of ash dieback have been recorded on F. excelsior in Slovakia since 2004. This study reports about the first natural occurrence of H. fraxineus on F. angustifolia in Slovakia. The field investigation was carried out in 2014. The segments of diseased shoots and last year’s petioles were collected in clonal seed orchard situated in southwest part of the country. The fungus was isolated from infected host tissue and identified using molecular techniques (DNA extraction from pure cultures and apothecia, conventional PCR).}, keywords = {}, pubstate = {published}, tppubtype = {article} } The fungus Hymenoscyphus fraxineus is responsible for dieback of common ash (Fraxinus excelsior) and in some parts of Europe also of narrow-leaved ash (F. angustifolia). The first symptoms of ash dieback have been recorded on F. excelsior in Slovakia since 2004. This study reports about the first natural occurrence of H. fraxineus on F. angustifolia in Slovakia. The field investigation was carried out in 2014. The segments of diseased shoots and last year’s petioles were collected in clonal seed orchard situated in southwest part of the country. The fungus was isolated from infected host tissue and identified using molecular techniques (DNA extraction from pure cultures and apothecia, conventional PCR). |
Pastirčáková, K; Shin, H D Contribution to the pathogenic fungi on woody plants in North Korea Journal Article Phytotaxa, 299 (2), pp. 185-198, 2017, ISSN: 1179-3155. @article{K.2017, title = {Contribution to the pathogenic fungi on woody plants in North Korea}, author = {K. Pastirčáková and H.D. Shin}, url = {https://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.299.2.3}, doi = {10.11646/phytotaxa.299.2.3}, issn = {1179-3155}, year = {2017}, date = {2017-03-21}, journal = {Phytotaxa}, volume = {299}, number = {2}, pages = {185-198}, abstract = {This paper presents the first checklist of pathogenic fungi on woody plants in North Korea. The checklist enumerates 146 species of fungi belonging to 79 genera, growing on 108 host plants belonging to 58 genera and 35 families. Two hosts and 57 fungal species represent first records in North Korea.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This paper presents the first checklist of pathogenic fungi on woody plants in North Korea. The checklist enumerates 146 species of fungi belonging to 79 genera, growing on 108 host plants belonging to 58 genera and 35 families. Two hosts and 57 fungal species represent first records in North Korea. |
Ostrovský, R; Kobza, M; Gažo, J Extensively damaged trees tested with acoustic tomography considering tree stability in urban greenery Journal Article Trees Structure and Function, 31 (3), pp. 1015-1023, 2017, ISSN: 1432-2285. @article{Ostrovský2017, title = {Extensively damaged trees tested with acoustic tomography considering tree stability in urban greenery}, author = {R. Ostrovský and M. Kobza and J. Gažo}, url = {https://link.springer.com/article/10.1007%2Fs00468-017-1526-6}, doi = {10.1007/s00468-017-1526-6}, issn = {1432-2285}, year = {2017}, date = {2017-02-07}, journal = {Trees Structure and Function}, volume = {31}, number = {3}, pages = {1015-1023}, abstract = {Objectives of study were to assess the accuracy and reliability of the acoustic tomography technique for detecting internal structural defects compared to visual assessment on extensively damaged trees of five species in urban greenery. Tomography was realized by Fakopp 3D acoustic tomograph tool. Several types of structural defects were determined, such as heartwood and sapwood decay, internal and lateral cracks, ring shake and hollow. Acoustic tomography inspection revealed correct detection of damage in all disc samples involved in study. Accuracy of damaged area determination reached 90%. Total accuracy determination for both area and location of damage was 83%. Overestimation of damaged area was observed in eight samples, contrary to seven underestimated samples. Difference in estimated false-positive area in comparison to false-negative area was minimal. Irregularity of cross section shape does not affect the final accuracy of tomograph. Accuracy is not influenced by diameter of tree trunk. We determined strong positive correlation between real area of damage and results of tomography (r = 0.75; p = 0.001). Acoustic tomography provides satisfactory accuracy in damage area determination inside tree trunk and for overall tree stability assessment on even extensively damaged trees in urban greenery.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Objectives of study were to assess the accuracy and reliability of the acoustic tomography technique for detecting internal structural defects compared to visual assessment on extensively damaged trees of five species in urban greenery. Tomography was realized by Fakopp 3D acoustic tomograph tool. Several types of structural defects were determined, such as heartwood and sapwood decay, internal and lateral cracks, ring shake and hollow. Acoustic tomography inspection revealed correct detection of damage in all disc samples involved in study. Accuracy of damaged area determination reached 90%. Total accuracy determination for both area and location of damage was 83%. Overestimation of damaged area was observed in eight samples, contrary to seven underestimated samples. Difference in estimated false-positive area in comparison to false-negative area was minimal. Irregularity of cross section shape does not affect the final accuracy of tomograph. Accuracy is not influenced by diameter of tree trunk. We determined strong positive correlation between real area of damage and results of tomography (r = 0.75; p = 0.001). Acoustic tomography provides satisfactory accuracy in damage area determination inside tree trunk and for overall tree stability assessment on even extensively damaged trees in urban greenery. |
Ondrušková, E; Hečková, Z; Kádasi-Horáková, M; Koltay, A; Ostrovský, R; Pažitný, J; Adamčíková, K Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia Journal Article European Journal of Plant Pathology, 148 (2), pp. 283-294, 2017, ISSN: 0929-1873. @article{Ondrušková2017, title = {Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia}, author = {E. Ondrušková and Z. Hečková and M. Kádasi-Horáková and A. Koltay and R. Ostrovský and J. Pažitný and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s10658-016-1088-2}, doi = {10.1007/s10658-016-1088-2}, issn = {0929-1873}, year = {2017}, date = {2017-01-01}, journal = {European Journal of Plant Pathology}, volume = {148}, number = {2}, pages = {283-294}, abstract = {The occurrence and distribution of Dothistroma needle blight (DNB) on Pinus mugo was studied in 2014–2015 around the Slovakia. In total, 42 localities were investigated both native and planted ones. Symptoms of DNB were observed on 35 localities only on planted shrubs. All these 35 localities are new P. mugo DNB stands. No DNB symptoms were observed in natural and naturally regenerated plantations. DNAwas extracted from a total of 236 isolates and eight needle samples. Based on the ITS-rDNA comparisons and using species specific primers, both pathogenic Dothistroma species were detected: D. septosporum and D. pini. Isolates of D. septosporum had ITS sequences identical to D. septosporum from Europe and both mating types were identified with slight predominance of MAT2. The ratio of D. septosporum mating types varies significantly between sites, ranging from an equal proportion of each mating type to single mating type populations. D. pini ITS sequence grouped with D. pini from Ukraine, Russia and Switzerland and only MAT2 was found.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The occurrence and distribution of Dothistroma needle blight (DNB) on Pinus mugo was studied in 2014–2015 around the Slovakia. In total, 42 localities were investigated both native and planted ones. Symptoms of DNB were observed on 35 localities only on planted shrubs. All these 35 localities are new P. mugo DNB stands. No DNB symptoms were observed in natural and naturally regenerated plantations. DNAwas extracted from a total of 236 isolates and eight needle samples. Based on the ITS-rDNA comparisons and using species specific primers, both pathogenic Dothistroma species were detected: D. septosporum and D. pini. Isolates of D. septosporum had ITS sequences identical to D. septosporum from Europe and both mating types were identified with slight predominance of MAT2. The ratio of D. septosporum mating types varies significantly between sites, ranging from an equal proportion of each mating type to single mating type populations. D. pini ITS sequence grouped with D. pini from Ukraine, Russia and Switzerland and only MAT2 was found. |
Ivanová, H Morphological features of Camarosporium pini – the fungus associated to health state degradation in Austrian and Ponderosa pine Journal Article Folia Oecologica, 44 (1), pp. 54-57, 2017, ISSN: 1336-5266. @article{Ivanová2017b, title = {Morphological features of \textit{Camarosporium pini} – the fungus associated to health state degradation in Austrian and Ponderosa pine}, author = {H. Ivanová}, url = {http://ife.sk/wp-content/uploads/2016/10/foecol-2017-0007.pdf}, doi = {10.1515/foecol-2017-0007}, issn = {1336-5266}, year = {2017}, date = {2017-01-01}, journal = {Folia Oecologica}, volume = {44}, number = {1}, pages = {54-57}, abstract = {The subject of this study is escalated occurrence of the pathogenic fungus Camarosporium pini in the needle tissue of symptomatic trees P. nigra and P. ponderosa var. jeffreyi growing in urbanized settings and parks. C. pini induces severe infections and initiates a blight and premature loss of second-year foliage in pine trees. The fungus was identified microscopically and on base of morphological keys. The affected needles displayed a distinct bluish-grey necrotic band in the centre. On the surface of infected needles, there were formed pycnidia producing brown, oval conidia with three transversal and one or two vertical walls. Disease symptoms, some important characteristics in pure culture, and distinctive morphological features of C. pini associated to the health state degradation in Austrian and Ponderosa pine are described and compared. Cumulative effects of these stressful biotic and various abiotic factors may explain the current situation concerning the decline in the P. nigra and P. ponderosa var. jeffreyi in Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The subject of this study is escalated occurrence of the pathogenic fungus Camarosporium pini in the needle tissue of symptomatic trees P. nigra and P. ponderosa var. jeffreyi growing in urbanized settings and parks. C. pini induces severe infections and initiates a blight and premature loss of second-year foliage in pine trees. The fungus was identified microscopically and on base of morphological keys. The affected needles displayed a distinct bluish-grey necrotic band in the centre. On the surface of infected needles, there were formed pycnidia producing brown, oval conidia with three transversal and one or two vertical walls. Disease symptoms, some important characteristics in pure culture, and distinctive morphological features of C. pini associated to the health state degradation in Austrian and Ponderosa pine are described and compared. Cumulative effects of these stressful biotic and various abiotic factors may explain the current situation concerning the decline in the P. nigra and P. ponderosa var. jeffreyi in Slovakia. |
Ivanová, Helena; Hamarová, Ľudmila; Pristaš, Peter Clonostachys rosea associated with ponderosa and Coulter pine needles in Slovakia Journal Article Biologia, 72 (11), pp. 1258—1263, 2017, ISSN: ISSN 0006-3088. @article{Ivanová2017, title = {Clonostachys rosea associated with ponderosa and Coulter pine needles in Slovakia}, author = {Helena Ivanová and Ľudmila Hamarová and Peter Pristaš}, doi = {https://doi.org/10.2478/s11756-018-0071-0}, issn = {ISSN 0006-3088}, year = {2017}, date = {2017-00-00}, journal = {Biologia}, volume = {72}, number = {11}, pages = {1258—1263}, abstract = {Clonostachys rosea is an important biological control agent against various plant pathogenic fungi. We collected – necrotic ponderosa and Coulter pine needles and obtained C. rosea – like fungal colonies. Cultures were identified as C. rosea and their morphological characteristics were described.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Clonostachys rosea is an important biological control agent against various plant pathogenic fungi. We collected – necrotic ponderosa and Coulter pine needles and obtained C. rosea – like fungal colonies. Cultures were identified as C. rosea and their morphological characteristics were described. |
2016 |
Adamčík, S; Jančovičová, S; Looney, B P; Adamčíková, K; Birkebak, J M; Moreau, P A; Vizziny, A; Matheny, P B Circumscription of species in the Hodophilus foetens complex (Clavariaceae, Agaricales) in Europe Journal Article Mycological Progress, 16 (1), pp. 47–62, 2016, ISSN: 1617-416X. @article{Adamčík2017, title = {Circumscription of species in the Hodophilus foetens complex (Clavariaceae, Agaricales) in Europe}, author = {S. Adamčík and S. Jančovičová and B.P. Looney and K. Adamčíková and J.M. Birkebak and P.A. Moreau and A. Vizziny and P.B. Matheny}, doi = {DOI 10.1007/s11557-016-1249-x}, issn = {1617-416X}, year = {2016}, date = {2016-12-12}, journal = {Mycological Progress}, volume = {16}, number = {1}, pages = {47–62}, abstract = {Four European Hodophilus species with an odour similar to naphthalene, a strong unpleasant odour similar to that of mothballs, are recognized based on sequence and/or morphological data. The traditional concept defines Ho. foetens as the only Hodophilus species with a naphthalene odour in Europe. This name is now assigned to one of the studied species based on morphological examination of the holotype specimen. A recently collected specimen is proposed as the epitype. The other three species with a naphthalene odour are described here as new: Ho. pallidus, Ho. subfoetens and Ho. tenuicystidiatus. They are distinguishable in the field based on a combination of lamellae number and colour of basidiomata. All four species are grouped in the Ho. foetens superclade, one of two superclades, together with the Ho. micaceus superclade, in the genus Hodophilus. All are different species from North American taxa with a naphthalene-like odour recognised in a previous study. The Ho. foetens superclade also includes one species identified as Ho. atropunctus that does not have a distinctive odour. The type collection of Ho. albofloccipes, a recently described European species with a naphthalene odour, is placed together with some collections without a distinctive odour in the Ho. micaceus superclade.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Four European Hodophilus species with an odour similar to naphthalene, a strong unpleasant odour similar to that of mothballs, are recognized based on sequence and/or morphological data. The traditional concept defines Ho. foetens as the only Hodophilus species with a naphthalene odour in Europe. This name is now assigned to one of the studied species based on morphological examination of the holotype specimen. A recently collected specimen is proposed as the epitype. The other three species with a naphthalene odour are described here as new: Ho. pallidus, Ho. subfoetens and Ho. tenuicystidiatus. They are distinguishable in the field based on a combination of lamellae number and colour of basidiomata. All four species are grouped in the Ho. foetens superclade, one of two superclades, together with the Ho. micaceus superclade, in the genus Hodophilus. All are different species from North American taxa with a naphthalene-like odour recognised in a previous study. The Ho. foetens superclade also includes one species identified as Ho. atropunctus that does not have a distinctive odour. The type collection of Ho. albofloccipes, a recently described European species with a naphthalene odour, is placed together with some collections without a distinctive odour in the Ho. micaceus superclade. |
Barta, M; Bibeň, T Stephanitis takeyai and S. rhododendri (Heteroptera: Tingidae) in Slovakia: first record and economic importance Journal Article Plant Protection Research, 56 (2), pp. 193-198, 2016, ISBN: 1899-007X. @article{Barta2016b, title = {\textit{Stephanitis takeyai} and \textit{S. rhododendri} (Heteroptera: \textit{Tingidae}) in Slovakia: first record and economic importance}, author = {M. Barta and T. Bibeň}, url = {https://www.degruyter.com/view/j/jppr.2016.56.issue-2/jppr-2016-0022/jppr-2016-0022.xml}, doi = {DOI: 10.1515/jppr-2016-0022}, isbn = {1899-007X}, year = {2016}, date = {2016-07-08}, journal = {Plant Protection Research}, volume = {56}, number = {2}, pages = {193-198}, abstract = {This is the first report on the occurrence of andromeda lace bug, Stephanitis (Stephanitis) takeyai Drake and Maa, 1955, and rhododendron lace bug, Stephanitis (Stephanitis) rhododendri Horvath, 1905, in Slovakia. Syntopic colonies of both species were found on rhododendron shrubs (Rhododendron sp.) in south-western Slovakia in 2015. The feeding of the lace bugs resulted in damage to infested rhododendrons. Leaves turned yellow and brown, prematurely dropped what led to continuous drying up of twigs and the whole plants. Details on morphology of adult stages of the two species, description of damage symptoms and economic importance of these pests are presented and discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This is the first report on the occurrence of andromeda lace bug, Stephanitis (Stephanitis) takeyai Drake and Maa, 1955, and rhododendron lace bug, Stephanitis (Stephanitis) rhododendri Horvath, 1905, in Slovakia. Syntopic colonies of both species were found on rhododendron shrubs (Rhododendron sp.) in south-western Slovakia in 2015. The feeding of the lace bugs resulted in damage to infested rhododendrons. Leaves turned yellow and brown, prematurely dropped what led to continuous drying up of twigs and the whole plants. Details on morphology of adult stages of the two species, description of damage symptoms and economic importance of these pests are presented and discussed. |
Adamčík, S; Looney, B P; Birkebak, J M; Jančovičová, S; Adamčíková, K; Marhold, K; Matheny, P B Circumscription of species of Hodophilus (Clavariaceae, Agaricales) in North America with naphthalene odours Journal Article Botany, 94 (10), pp. 941-956, 2016, ISSN: 1916-2804. @article{ADAMČÍK2016, title = {Circumscription of species of Hodophilus (Clavariaceae, Agaricales) in North America with naphthalene odours}, author = {S. Adamčík and B.P. Looney and J.M. Birkebak and S. Jančovičová and K. Adamčíková and K. Marhold and P.B. Matheny}, doi = {dx.doi.org/10.1139/cjb-2016-0091}, issn = {1916-2804}, year = {2016}, date = {2016-06-14}, journal = {Botany}, volume = {94}, number = {10}, pages = {941-956}, abstract = {Five North American Hodophilus species with naphthalene-like odours are now recognized based on sequence and (or) morphological data and molecular annotation of type collections. Two well-supported eastern North American species do not match any of the studied types and are described here as new: Hodophilus hesleri and Hodophilus smithii. The previously described Hodophilus paupertinus is found to represent an autonomous species and appears restricted to western North America. Hodophilus subfuscescens is found to be an independent lineage in eastern North America. A morphological type study of Hodophilus peckianus shows that it is a distinct species and not represented among recent collections. Multilocus phylogenetic analyses of European and North American material of species with naphthalene odours reveal no species with transatlantic distributions. Overall, Hodophilus comprises two superclades (the Hodophilus foetens superclade and the Hodophilus micaceus superclade) and 16 terminal clades that correspond to phylogenetic species. This study introduces a new approach for morphological delimitation of agaricoid Clavariaceae combining shape and dimensions of particular elements in the pileipellis and caulocystidia. All previously described taxa included in this study, which were previously treated in the genera Hygrophorus, Camarophyllopsis, or Hygrotrama, are formally transferred to Hodophilus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five North American Hodophilus species with naphthalene-like odours are now recognized based on sequence and (or) morphological data and molecular annotation of type collections. Two well-supported eastern North American species do not match any of the studied types and are described here as new: Hodophilus hesleri and Hodophilus smithii. The previously described Hodophilus paupertinus is found to represent an autonomous species and appears restricted to western North America. Hodophilus subfuscescens is found to be an independent lineage in eastern North America. A morphological type study of Hodophilus peckianus shows that it is a distinct species and not represented among recent collections. Multilocus phylogenetic analyses of European and North American material of species with naphthalene odours reveal no species with transatlantic distributions. Overall, Hodophilus comprises two superclades (the Hodophilus foetens superclade and the Hodophilus micaceus superclade) and 16 terminal clades that correspond to phylogenetic species. This study introduces a new approach for morphological delimitation of agaricoid Clavariaceae combining shape and dimensions of particular elements in the pileipellis and caulocystidia. All previously described taxa included in this study, which were previously treated in the genera Hygrophorus, Camarophyllopsis, or Hygrotrama, are formally transferred to Hodophilus. |
Schemmer, R; Chládeková, P; Medo, J; Barta, M Plant Protection Science, 52 (3), pp. 199-208, 2016, ISSN: 1805-9341. @article{Schemmer2016, title = {Natural prevalence of entomopathogenic fungi in hibernating pupae of \textit{Cameraria ohridella} (Lepidoptera: \textit{Gracillariidae}) and virulence of selected isolates}, author = {R. Schemmer and P. Chládeková and J. Medo and M. Barta}, url = {http://www.agriculturejournals.cz/publicFiles/189075.pdf}, doi = {doi: 10.17221/110/2015-PPS}, issn = {1805-9341}, year = {2016}, date = {2016-05-17}, journal = {Plant Protection Science}, volume = {52}, number = {3}, pages = {199-208}, abstract = {Spontaneous infection of hibernating Cameraria ohridella pupae by entomopathogenic fungi was monitored at two localities of south-western Slovakia and efficacy of selected fungal isolates against the pupae was evaluated in laboratory. Natural prevalence of fungal infection in pupal populations was low (< 7%) and varied depending upon collecting date, locality, year, and pathogen. Ten isolates of three fungal species, Beauveria pseudobassiana, Isaria fumosorosea, and I. farinosa, isolated from the naturally infected pupae and three isolates of non-insect origin of two fungi, B. bassiana and Metarhizium anisopliae, were screened for colony growth, conidial production in vitro, and virulence to C. ohridella pupae. A significant variability in the evaluated traits was detected among the isolates. Pupae demonstrated vulnerability to all the isolates irrespective of their origin, however virulence of isolates varied significantly. I. fumosorosea was the most virulent fungus with median lethal concentration of 0.09 × 104 conidia/ml (isolate CO10-IFu) and mean survival time of pupae of 7.14 days (isolate CO8-IFu). No correlation between conidial yield of isolates and fungal virulence was observed, but a moderately strong relationship was detected between virulence and mycelial growth rate of isolates. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Spontaneous infection of hibernating Cameraria ohridella pupae by entomopathogenic fungi was monitored at two localities of south-western Slovakia and efficacy of selected fungal isolates against the pupae was evaluated in laboratory. Natural prevalence of fungal infection in pupal populations was low (< 7%) and varied depending upon collecting date, locality, year, and pathogen. Ten isolates of three fungal species, Beauveria pseudobassiana, Isaria fumosorosea, and I. farinosa, isolated from the naturally infected pupae and three isolates of non-insect origin of two fungi, B. bassiana and Metarhizium anisopliae, were screened for colony growth, conidial production in vitro, and virulence to C. ohridella pupae. A significant variability in the evaluated traits was detected among the isolates. Pupae demonstrated vulnerability to all the isolates irrespective of their origin, however virulence of isolates varied significantly. I. fumosorosea was the most virulent fungus with median lethal concentration of 0.09 × 104 conidia/ml (isolate CO10-IFu) and mean survival time of pupae of 7.14 days (isolate CO8-IFu). No correlation between conidial yield of isolates and fungal virulence was observed, but a moderately strong relationship was detected between virulence and mycelial growth rate of isolates. |
Kollár, J; Barta, M The first record of tulip tree aphid, Illinoia liriodendri (Hemiptera: Aphididae), from Slovakia – Short Communication Journal Article Plant Protection Science, 52 (2), pp. 142-146, 2016, ISSN: 1212-2580. @article{Kollár2016, title = {The first record of tulip tree aphid, \textit{Illinoia liriodendri} (Hemiptera: \textit{Aphididae}), from Slovakia – Short Communication}, author = {J. Kollár and M. Barta}, url = {http://www.agriculturejournals.cz/publicFiles/178824.pdf}, doi = {DOI: 10.17221/109/2015-PPS}, issn = {1212-2580}, year = {2016}, date = {2016-05-17}, journal = {Plant Protection Science}, volume = {52}, number = {2}, pages = {142-146}, abstract = {The tulip tree aphid, Illinoia liriodendri, is reported in Slovakia for the first time. Colonies of I. liriodendri were found on tulip trees, Liriodendron tulipifera, in two locations of south-western Slovakia in 2014 and 2015. Colonies of nymphs and viviparous females fed on the underside of leaves with no significant symptoms of damage to tulip trees. In this short study, the first report of the aphid from Slovakia is presented and original illustrations with results of morphometric analysis of alate viviparous females are provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The tulip tree aphid, Illinoia liriodendri, is reported in Slovakia for the first time. Colonies of I. liriodendri were found on tulip trees, Liriodendron tulipifera, in two locations of south-western Slovakia in 2014 and 2015. Colonies of nymphs and viviparous females fed on the underside of leaves with no significant symptoms of damage to tulip trees. In this short study, the first report of the aphid from Slovakia is presented and original illustrations with results of morphometric analysis of alate viviparous females are provided. |
Pastirčáková, K; Jankovics, T; Komáromi, J; Pintye, A; Pastirčák, M Genetic diversity and host range of powdery mildews on Papaveraceae Journal Article Mycological Progress, 15 (4, article 36), pp. 1-18, 2016, ISSN: 1617-416X. @article{K.2016, title = {Genetic diversity and host range of powdery mildews on Papaveraceae}, author = {K. Pastirčáková and T. Jankovics and J. Komáromi and A. Pintye and M. Pastirčák}, url = {https://link.springer.com/article/10.1007/s11557-016-1178-8}, doi = {10.1007/s11557-016-1178-8}, issn = {1617-416X}, year = {2016}, date = {2016-04-08}, journal = {Mycological Progress}, volume = {15}, number = {4, article 36}, pages = {1-18}, abstract = {Because of the strong morphological similarity of the powdery mildew fungi that infect papaveraceous hosts, a total of 39 samples were studied to reveal the phylogeny and host range of these fungi. ITS and 28S sequence analyses revealed that the isolates identified earlier as Erysiphe cruciferarum on papaveraceous hosts represent distinct lineages and differ from that of E. cruciferarum sensu stricto on brassicaceous hosts. The taxonomic status of the anamorph infecting Eschscholzia californica was revised, and therefore, a new species name, Erysiphe eschscholziae, is proposed. The taxonomic position of the Pseudoidium anamorphs infecting Glaucium flavum, Meconopsis cambrica, Papaver dubium, and Stylophorum diphyllum remain unclear. This study revealed that Erysiphe macleayae exhibits a specific host range different from that of E. cruciferarum, the common pathogen of papaveraceous hosts. Although E. macleayae occurred naturally on Macleaya cordata, Macleaya microcarpa, M. cambrica, and Chelidonium majus only, our inoculation tests revealed that the fungus was capable of infecting Argemone grandiflora, Glaucium corniculatum, Papaver rhoeas, and Papaver somniferum, indicating that these plant species may also be taken into account as potential hosts. Erysiphe cruciferarum originating from P. somniferum was not able to infect A. grandiflora, C. majus, E. californica, M. cordata, and P. rhoeas. The emergence of E. macleayae on M. microcarpa is reported here for the first time from the Czech Republic and Slovakia. The appearance of chasmothecia of E. macleayae on C. majus in Slovakia was reported, as well. Erysiphe cruciferarum was identified on G. corniculatum and reported here for the first time from Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Because of the strong morphological similarity of the powdery mildew fungi that infect papaveraceous hosts, a total of 39 samples were studied to reveal the phylogeny and host range of these fungi. ITS and 28S sequence analyses revealed that the isolates identified earlier as Erysiphe cruciferarum on papaveraceous hosts represent distinct lineages and differ from that of E. cruciferarum sensu stricto on brassicaceous hosts. The taxonomic status of the anamorph infecting Eschscholzia californica was revised, and therefore, a new species name, Erysiphe eschscholziae, is proposed. The taxonomic position of the Pseudoidium anamorphs infecting Glaucium flavum, Meconopsis cambrica, Papaver dubium, and Stylophorum diphyllum remain unclear. This study revealed that Erysiphe macleayae exhibits a specific host range different from that of E. cruciferarum, the common pathogen of papaveraceous hosts. Although E. macleayae occurred naturally on Macleaya cordata, Macleaya microcarpa, M. cambrica, and Chelidonium majus only, our inoculation tests revealed that the fungus was capable of infecting Argemone grandiflora, Glaucium corniculatum, Papaver rhoeas, and Papaver somniferum, indicating that these plant species may also be taken into account as potential hosts. Erysiphe cruciferarum originating from P. somniferum was not able to infect A. grandiflora, C. majus, E. californica, M. cordata, and P. rhoeas. The emergence of E. macleayae on M. microcarpa is reported here for the first time from the Czech Republic and Slovakia. The appearance of chasmothecia of E. macleayae on C. majus in Slovakia was reported, as well. Erysiphe cruciferarum was identified on G. corniculatum and reported here for the first time from Slovakia. |
Barta, M Biology and temperature requirements of the invasive seed bug Leptoglossus occidentalis (Heteroptera: Coreidae) in Europe. Journal Article Journal of Pest Science, 89 (1), pp. 31-44, 2016, ISSN: 1612-4758. @article{Barta2016, title = {Biology and temperature requirements of the invasive seed bug \textit{Leptoglossus occidentalis} (Heteroptera: Coreidae) in Europe.}, author = {M. Barta}, url = {https://link.springer.com/article/10.1007/s10340-015-0673-z}, doi = {10.1007/s10340-015-0673-z}, issn = {1612-4758}, year = {2016}, date = {2016-03-01}, journal = {Journal of Pest Science}, volume = {89}, number = {1}, pages = {31-44}, abstract = {Leptoglossus occidentalis, a species native to North America, has been expanding through European conifer forests. The fast spread across the European continent demonstrates its great invasive potential and poses a threat to conifer seed orchards. To better understand its potential distribution and population growth in infested regions, the temperature-dependent development in laboratory as well as life history, phenology, and winter survival were investigated in southern Slovakia during 2009-2011. The lower temperature thresholds to complete embryonic and postembryonic development estimated with the linear model were 13.38 and 14.37 degrees C, respectively. The number of degree-days required for completion of development from egg to adult estimated by the linear model was 533 degrees C. Overwintered adults or late nymphal instars became active in mid-March, oviposition was not observed before mid-April, and nymphs passing through five stages reached adulthood after mid-July. Individual females laid on average 31 eggs (max 78). The second generation started in August but did not complete its development till winter, although part of the 5th instar nymphs did survive until spring. Using temperature data of 41 localities across Europe and cumulative degree-days for complete life cycle, a prediction of the potential number of generations per year was made. In conditions of southern Slovakia L. occidentalis was univoltine, but up to four generations per year were estimated depending on the locality in Europe.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Leptoglossus occidentalis, a species native to North America, has been expanding through European conifer forests. The fast spread across the European continent demonstrates its great invasive potential and poses a threat to conifer seed orchards. To better understand its potential distribution and population growth in infested regions, the temperature-dependent development in laboratory as well as life history, phenology, and winter survival were investigated in southern Slovakia during 2009-2011. The lower temperature thresholds to complete embryonic and postembryonic development estimated with the linear model were 13.38 and 14.37 degrees C, respectively. The number of degree-days required for completion of development from egg to adult estimated by the linear model was 533 degrees C. Overwintered adults or late nymphal instars became active in mid-March, oviposition was not observed before mid-April, and nymphs passing through five stages reached adulthood after mid-July. Individual females laid on average 31 eggs (max 78). The second generation started in August but did not complete its development till winter, although part of the 5th instar nymphs did survive until spring. Using temperature data of 41 localities across Europe and cumulative degree-days for complete life cycle, a prediction of the potential number of generations per year was made. In conditions of southern Slovakia L. occidentalis was univoltine, but up to four generations per year were estimated depending on the locality in Europe. |
Zúbrik, M; Hajek, A; Pilarska, D; Špilda, I; Georgiev, G; Hrašovec, B; Hirka, A; Goertz, D; Hoch, G; Barta, M; Saniga, M; Kunca, A; Nikolov, C; Vakula, J; Galko, J; Pilarski, P; Csóka, G The potential for Entomophaga maimaiga to regulate gypsy moth Lymantria dispar (L.) (Lepidoptera; Erebidae) in Europe Journal Article Journal of Applied Entomology, 140 (8), pp. 565–579, 2016, ISSN: 1439-0418. @article{Zúbrik2016, title = {The potential for \textit{Entomophaga maimaiga} to regulate gypsy moth \textit{Lymantria dispar} (L.) (Lepidoptera; \textit{Erebidae}) in Europe}, author = {M. Zúbrik and A. Hajek and D. Pilarska and I. Špilda and G. Georgiev and B. Hrašovec and A. Hirka and D. Goertz and G. Hoch and M. Barta and M. Saniga and A. Kunca and C. Nikolov and J. Vakula and J. Galko and P. Pilarski and G. Csóka}, url = {http://onlinelibrary.wiley.com/doi/10.1111/jen.12295/epdf}, doi = {doi:10.1111/jen.12295}, issn = {1439-0418}, year = {2016}, date = {2016-02-13}, journal = {Journal of Applied Entomology}, volume = {140}, number = {8}, pages = {565–579}, abstract = {Gypsy moth, Lymantria dispar L., is one of the most important pests of deciduous trees in Europe. In regular cycles, it causes large-scale defoliation mostly of oak, Quercus spp., forests. Government authorities in the most infested countries in Europe conduct large-scale applications of pesticides against gypsy moth. In 1999, a new natural enemy, the entomopathogenic fungus Entomophaga maimaiga, was successfully introduced into a gypsy moth population in Bulgaria. Recent investigations suggest that now E. maimaiga is quickly spreading in Europe. Herein, past studies are reviewed regarding this fungus with special emphasis on its potential for becoming an important factor regulating gypsy moth populations in Europe, focusing on the host's population dynamics in relation to the fungus, the influence of environmental conditions on fungal activity, the influence of E. maimaiga on the native entomofauna, including other gypsy moth natural enemies, and spread of the fungus. Based on this analysis, the potential of E. maimaiga for providing control in European gypsy moth populations is estimated.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Gypsy moth, Lymantria dispar L., is one of the most important pests of deciduous trees in Europe. In regular cycles, it causes large-scale defoliation mostly of oak, Quercus spp., forests. Government authorities in the most infested countries in Europe conduct large-scale applications of pesticides against gypsy moth. In 1999, a new natural enemy, the entomopathogenic fungus Entomophaga maimaiga, was successfully introduced into a gypsy moth population in Bulgaria. Recent investigations suggest that now E. maimaiga is quickly spreading in Europe. Herein, past studies are reviewed regarding this fungus with special emphasis on its potential for becoming an important factor regulating gypsy moth populations in Europe, focusing on the host's population dynamics in relation to the fungus, the influence of environmental conditions on fungal activity, the influence of E. maimaiga on the native entomofauna, including other gypsy moth natural enemies, and spread of the fungus. Based on this analysis, the potential of E. maimaiga for providing control in European gypsy moth populations is estimated. |
Ivanová, H; Pristaš, P; Ondrušková, E Comparison of two Coniochaeta species (C. ligniaria and C. malacotricha) with a new pathogen of black pine needles - Sordaria macrospora Journal Article Plant Protection Science, 52 (1), pp. 18-25, 2016. @article{Ivanová2016, title = {Comparison of two Coniochaeta species (C. ligniaria and C. malacotricha) with a new pathogen of black pine needles - Sordaria macrospora}, author = {H. Ivanová and P. Pristaš and E. Ondrušková}, year = {2016}, date = {2016-01-01}, journal = {Plant Protection Science}, volume = {52}, number = {1}, pages = {18-25}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H Comparison of fungal endophytes Pestalotia funerea and Truncatella hartigii isolated from some species of genus Pinus in morphological characteristics of conidia and appendages Journal Article Journal of Forest Science, 62 (6), pp. 279-284, 2016. @article{Ivanová2016b, title = {Comparison of fungal endophytes Pestalotia funerea and Truncatella hartigii isolated from some species of genus Pinus in morphological characteristics of conidia and appendages}, author = {H. Ivanová}, year = {2016}, date = {2016-01-01}, journal = {Journal of Forest Science}, volume = {62}, number = {6}, pages = {279-284}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H; Hrehová, Ľ; Pristaš, P First confirmed report on Fusarium sporotrichioides on Pinus ponderosa var. jeffreyi in Slovakia Journal Article Plant Protection Science, 52 (4), pp. 250-253, 2016. @article{Ivanová2016b, title = {First confirmed report on Fusarium sporotrichioides on Pinus ponderosa var. jeffreyi in Slovakia}, author = {H. Ivanová and Ľ. Hrehová and P. Pristaš}, year = {2016}, date = {2016-01-01}, journal = {Plant Protection Science}, volume = {52}, number = {4}, pages = {250-253}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2015 |
Adamčíková, K; Kádasi-Horáková, M; Jankovský, L; Havrdová, L Identification of Hymenoscyphus fraxineus, the causal agent of ash dieback in Slovakia Journal Article Biologia, 70 (5), pp. 559–564, 2015. @article{K.2015, title = {Identification of \textit{Hymenoscyphus fraxineus}, the causal agent of ash dieback in Slovakia}, author = {K. Adamčíková and M. Kádasi-Horáková and L. Jankovský and L. Havrdová}, url = {http://www.degruyter.com/view/j/biolog.2015.70.issue-5/biolog-2015-0075/biolog-2015-0075.xml}, doi = {10.1515/biolog-2015-0075}, year = {2015}, date = {2015-06-23}, journal = {Biologia}, volume = {70}, number = {5}, pages = {559–564}, abstract = {Symptoms of ash dieback have been recorded in Slovakia since 2004. The field sampling was carried out in 2013, included 59 segments of shoots and 10 and more petioles per locality from four localities. The causal agent of ash dieback, the hyphomycete Chalara fraxinea T. Kowalski, was isolated from Fraxinus excelsior L. from seven localities in Slovakia. The morphology of C. fraxinea isolates and the teleomorph Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz, Hosoya are described and ITS sequences are provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Symptoms of ash dieback have been recorded in Slovakia since 2004. The field sampling was carried out in 2013, included 59 segments of shoots and 10 and more petioles per locality from four localities. The causal agent of ash dieback, the hyphomycete Chalara fraxinea T. Kowalski, was isolated from Fraxinus excelsior L. from seven localities in Slovakia. The morphology of C. fraxinea isolates and the teleomorph Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz, Hosoya are described and ITS sequences are provided. |
Adamčíková, K; Ondrušková, E; Kádasi-Horáková, M; Botu, M; Kobza, M; Achim, G Distribution and population structure of the chestnut blight fungus in Romania Journal Article Plant Protection Science, 51 (3), pp. 141-149, 2015. @article{Adamčíková2015, title = {Distribution and population structure of the chestnut blight fungus in Romania}, author = {K. Adamčíková and E. Ondrušková and M. Kádasi-Horáková and M. Botu and M. Kobza and G. Achim}, url = {http://www.agriculturejournals.cz/publicFiles/157004.pdf}, doi = {10.17221/52/2014-PPS}, year = {2015}, date = {2015-01-01}, journal = {Plant Protection Science}, volume = {51}, number = {3}, pages = {141-149}, abstract = {The occurrence of chestnut blight (Cryphonectria parasitica) was studied in 2011-2012 at 13 locations in the main chestnut growing areas of Romania. Infections were detected at four localities. The symptoms and the fungus were detected on European chestnut (four localities) and also on oak trees (two localities). A total of 89 isolates of C. parasitica were isolated and characterised. Based on canker and isolate morphology (culture morphology and the Bavendamm test), both virulent and hypovirulent samples were isolated; hypovirulent isolates were found at only one locality. Two vegetative compatibility types corresponding to EU-12 and EU-2 were identified among isolates. Both mating types were found, with a dominance of MAT-1 in southern Romania and MAT-2 in northern Romania.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The occurrence of chestnut blight (Cryphonectria parasitica) was studied in 2011-2012 at 13 locations in the main chestnut growing areas of Romania. Infections were detected at four localities. The symptoms and the fungus were detected on European chestnut (four localities) and also on oak trees (two localities). A total of 89 isolates of C. parasitica were isolated and characterised. Based on canker and isolate morphology (culture morphology and the Bavendamm test), both virulent and hypovirulent samples were isolated; hypovirulent isolates were found at only one locality. Two vegetative compatibility types corresponding to EU-12 and EU-2 were identified among isolates. Both mating types were found, with a dominance of MAT-1 in southern Romania and MAT-2 in northern Romania. |
Kobza, M; Ostrovský, R Škodcovia gaštana jedlého [Pests of European chestnut] Book Chapter Cagáň, Ľ (Ed.): Choroby a škodcovia záhradníckych rastlín, pp. 301-307, SPU v Nitre, Nitra, 1, 2015, ISBN: 978-80-552-1448-1. @inbook{Kobza2015, title = {Škodcovia gaštana jedlého [Pests of European chestnut]}, author = {M. Kobza and R. Ostrovský}, editor = {Ľ. Cagáň}, isbn = {978-80-552-1448-1}, year = {2015}, date = {2015-01-01}, booktitle = {Choroby a škodcovia záhradníckych rastlín}, pages = {301-307}, publisher = {SPU v Nitre}, address = {Nitra}, edition = {1}, keywords = {}, pubstate = {published}, tppubtype = {inbook} } |
Kobza, M; Ostrovský, R Škodcovia orecha kráľovského [Pest of walnut] Book Chapter Cagáň, Ľ (Ed.): Choroby a škodcovia záhradníckych rastlín, pp. 293-299, SPU v Nitre, Nitra, 1, 2015, ISBN: 978-80-552-1448-1. @inbook{Kobza2015b, title = {Škodcovia orecha kráľovského [Pest of walnut]}, author = {M. Kobza and R. Ostrovský}, editor = {Ľ. Cagáň}, isbn = {978-80-552-1448-1}, year = {2015}, date = {2015-01-01}, booktitle = {Choroby a škodcovia záhradníckych rastlín}, pages = {293-299}, publisher = {SPU v Nitre}, address = {Nitra}, edition = {1}, keywords = {}, pubstate = {published}, tppubtype = {inbook} } |
Kobza, M; Ostrovský, R Škodcovia moruší [Pests of mulberries] Book Chapter Cagáň, Ľ (Ed.): Choroby a škodcovia záhradníckych rastlín, pp. 437-445, SPU v Nitre, Nitra, 1, 2015, ISBN: 978-80-552-1448-1. @inbook{Kobza2015b, title = {Škodcovia moruší [Pests of mulberries]}, author = {M. Kobza and R. Ostrovský}, editor = {Ľ. Cagáň}, isbn = {978-80-552-1448-1}, year = {2015}, date = {2015-01-01}, booktitle = {Choroby a škodcovia záhradníckych rastlín}, pages = {437-445}, publisher = {SPU v Nitre}, address = {Nitra}, edition = {1}, keywords = {}, pubstate = {published}, tppubtype = {inbook} } |
Ostrovský, R; Kobza, M Význam starostlivosti o dreviny vo verejnej zeleni Proceeding Ústav ekológie lesa SAV Zvolen, Pobočka biológie drevín Nitra, Nitra, 2015, ISBN: 978-80-89408-19-1. @proceedings{Ostrovský2015, title = {Význam starostlivosti o dreviny vo verejnej zeleni}, author = {R. Ostrovský and M. Kobza}, editor = {P. Hrubík and H. Ivanová}, isbn = {978-80-89408-19-1}, year = {2015}, date = {2015-01-01}, booktitle = {Zborník príspevkov z konferencie s medzinárodnou účasťou, 28. - 29. 4 2015, Nitra [Importance of care of trees in urban area: conference proceedings]}, pages = {159}, publisher = {Ústav ekológie lesa SAV Zvolen, Pobočka biológie drevín Nitra}, address = {Nitra}, keywords = {}, pubstate = {published}, tppubtype = {proceedings} } |
Ivanová, H Sordaria fimicola (Ascomycota, Sordariales) on Acer palmatum Journal Article Folia Oecologica, 42 (1), pp. 67-71, 2015. @article{Ivanová2015, title = {Sordaria fimicola (Ascomycota, Sordariales) on Acer palmatum}, author = {H. Ivanová}, year = {2015}, date = {2015-01-01}, journal = {Folia Oecologica}, volume = {42}, number = {1}, pages = {67-71}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H Fungi associated with a decline of Pinus nigra in urban greenery Journal Article Acta Fytotechnica et Zootechnica, 18 (2), pp. 36-43, 2015. @article{Ivanová2015b, title = {Fungi associated with a decline of Pinus nigra in urban greenery}, author = {H. Ivanová}, year = {2015}, date = {2015-01-01}, journal = {Acta Fytotechnica et Zootechnica}, volume = {18}, number = {2}, pages = {36-43}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H Sordaria fimicola (Ascomycota, Sordariales) on Acer palmatum Journal Article Folia Oecologica, 42 (1), pp. 67-71, 2015, ISSN: 1336-5266. @article{Ivanová2015b, title = {\textit{Sordaria fimicola} (Ascomycota, Sordariales) on \textit{Acer palmatum}}, author = {H. Ivanová}, url = {http://ife.sk/wp-content/uploads/2016/10/FO_v42_iss1_67to71.pdf}, issn = {1336-5266}, year = {2015}, date = {2015-01-01}, journal = {Folia Oecologica}, volume = {42}, number = {1}, pages = {67-71}, abstract = {During an investigation of the mycoflora of Japanese red maple trees growing in an urbanized area of Nitra, Slovakia, Sordaria fimicola (Roberge ex Desm.) Ces. & De Not) was isolated from affected leaves and branches of Acer palmatum ´Atropurpureum´ L. This fungus was associated with symptoms of brown wood discoloration and leaf spottiness of these trees. Sordaria fimicola was recorded for the first time on Acer palmatum ´Atropurpureum´ in Slovakia. The fungus is characterized by dark brown ascomata clothed with setae, fasciculate, unitunicate, cylindrical asci, and olivaceous to olivaceous-brown, one-celled, ellipsoidal, smooth-walled ascospores with a colourless basal germ pore and surrounded by a gelatinous sheath.}, keywords = {}, pubstate = {published}, tppubtype = {article} } During an investigation of the mycoflora of Japanese red maple trees growing in an urbanized area of Nitra, Slovakia, Sordaria fimicola (Roberge ex Desm.) Ces. & De Not) was isolated from affected leaves and branches of Acer palmatum ´Atropurpureum´ L. This fungus was associated with symptoms of brown wood discoloration and leaf spottiness of these trees. Sordaria fimicola was recorded for the first time on Acer palmatum ´Atropurpureum´ in Slovakia. The fungus is characterized by dark brown ascomata clothed with setae, fasciculate, unitunicate, cylindrical asci, and olivaceous to olivaceous-brown, one-celled, ellipsoidal, smooth-walled ascospores with a colourless basal germ pore and surrounded by a gelatinous sheath. |
2014 |
Uváčková, Ľ; Ondrušková, E; Danchenko, M; Škultéty, Ľ; Miernyk, J A; Hrubík, P; Hajduch, M Establishing a leaf proteome reference map for Ginkgo biloba provides insight into potential ethnobotanical uses Journal Article Journal of Agricultural and Food Chemistry, 62 (47), pp. 11547–11556, 2014. @article{Ľ.2014b, title = {Establishing a leaf proteome reference map for Ginkgo biloba provides insight into potential ethnobotanical uses}, author = {Ľ. Uváčková and E. Ondrušková and M. Danchenko and Ľ. Škultéty and J.A. Miernyk and P. Hrubík and M. Hajduch}, url = {https://www.ncbi.nlm.nih.gov/pubmed/25365400}, year = {2014}, date = {2014-11-26}, journal = {Journal of Agricultural and Food Chemistry}, volume = {62}, number = {47}, pages = {11547–11556}, abstract = {Ginkgo (Maidenhair tree, Ginkgo biloba L.), is an ancient medicinal and ornamental tree. However, systematic proteomics study of ginkgo leaves is still missing. Herein we performed initial study of ginkgo leaf proteome and established reference map. Proteins were isolated from fully developed mature leaves in biological triplicate and analyzed with two-dimensional gel electrophoresis in combination with tandem mass spectrometry and intensive bioinformatics. This approach reliably quantified 190 protein spots out of which 158 proteins were identified by mass spectrometry. These proteins were used to establish the reference map of ginkgo leaf proteome. Most of identified proteins were associated with Energy followed by Protein Destination and Storage. The identity of identified proteins confirmed accumulation of flavonoids and other phenolic compounds in mature leaves. This study also revealed proteins whose function is not yet known. Interestingly, second most abundant unknown protein was found to be similar to protein characterized in the king cobra. Proteins with unknown function detected in this study constitute pool of potential targets for metabolic engineering. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Ginkgo (Maidenhair tree, Ginkgo biloba L.), is an ancient medicinal and ornamental tree. However, systematic proteomics study of ginkgo leaves is still missing. Herein we performed initial study of ginkgo leaf proteome and established reference map. Proteins were isolated from fully developed mature leaves in biological triplicate and analyzed with two-dimensional gel electrophoresis in combination with tandem mass spectrometry and intensive bioinformatics. This approach reliably quantified 190 protein spots out of which 158 proteins were identified by mass spectrometry. These proteins were used to establish the reference map of ginkgo leaf proteome. Most of identified proteins were associated with Energy followed by Protein Destination and Storage. The identity of identified proteins confirmed accumulation of flavonoids and other phenolic compounds in mature leaves. This study also revealed proteins whose function is not yet known. Interestingly, second most abundant unknown protein was found to be similar to protein characterized in the king cobra. Proteins with unknown function detected in this study constitute pool of potential targets for metabolic engineering. |
Montalva, C; Barta, M; Rojas, E; Gutiérez, M; Valenzuela, E Neozygites species associated with aphids in Chile: current status and new reports Journal Article Mycotaxon, 129 (2), pp. 233-245, 2014, ISSN: 2154-8889. @article{Montalva2014, title = {\textit{Neozygites} species associated with aphids in Chile: current status and new reports}, author = {C. Montalva and M. Barta and E. Rojas and M. Gutiérez and E. Valenzuela}, url = {http://www.ingentaconnect.com/content/mtax/mt/2015/00000129/00000002/art00005}, doi = {DOI: https://doi.org/10.5248/129.233}, issn = {2154-8889}, year = {2014}, date = {2014-10-01}, journal = {Mycotaxon}, volume = {129}, number = {2}, pages = {233-245}, abstract = {Three species of Neozygites were recorded during a 2007–13 survey of the occurrence of the genus on aphids in Chile. Neozygites osornensis is known from recent studies, and N. fresenii and N. cinarae are reported as new records for Chile. Neozygites lageniformis, which was not found during the survey, had been recorded previously in Chile. Morphological descriptions, symptoms on infected insects, host spectrum, and geographical distribution of all four species are presented, and a key to Neozygites species associated with aphids in Chile is included. These fungi, which are important natural enemies of aphids, may be considered for future aphid pest control.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Three species of Neozygites were recorded during a 2007–13 survey of the occurrence of the genus on aphids in Chile. Neozygites osornensis is known from recent studies, and N. fresenii and N. cinarae are reported as new records for Chile. Neozygites lageniformis, which was not found during the survey, had been recorded previously in Chile. Morphological descriptions, symptoms on infected insects, host spectrum, and geographical distribution of all four species are presented, and a key to Neozygites species associated with aphids in Chile is included. These fungi, which are important natural enemies of aphids, may be considered for future aphid pest control. |
Pastirčáková, K; Pastirčák, M; Adamčiková, K; Bouznad, Z; Kedad, A; El_Guilli, M; Diminić, D; Hofte, M Global distribution of Erysiphe platani: new records, teleomorph formation and re-examination of herbarium collections Journal Article Cryptogamie, Mycologie, 35 (2), pp. 163-176, 2014, ISSN: 0181-1584. @article{K.2014, title = {Global distribution of \textit{Erysiphe platani}: new records, teleomorph formation and re-examination of herbarium collections}, author = {K. Pastirčáková and M. Pastirčák and K. Adamčiková and Z. Bouznad and A. Kedad and M. El_Guilli and D. Diminić and M. Hofte}, url = {http://www.bioone.org/doi/abs/10.7872/crym.v35.iss2.2014.163?journalCode=crym}, doi = {10.7872/crym.v35.iss2.2014.163}, issn = {0181-1584}, year = {2014}, date = {2014-06-01}, journal = {Cryptogamie, Mycologie}, volume = {35}, number = {2}, pages = {163-176}, abstract = {A global survey of the spread of the Platanus powdery mildew, Erysiphe platani, has been carried out. E. platani teleomorph formation was recorded in countries where the fungus anamorph has been present for several years. The first findings of chasmothecia were recorded in Austria, Czech Republic, France, Italy and Slovakia. New records of E. platani (including the teleomorph) were found in Belgium, Croatia and Denmark. The occurrence of this fungus in Sweden and in two countries of North Africa (Algeria and Morocco) was confirmed. Descriptions of morphological features, illustrations, and worldwide distribution of E. platani are provided. Herbarium collections of powdery mildews on Platanus spp. were re-examined and revised. The occurrence of Phyllactinia guttata on Platanus is discussed and questioned.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A global survey of the spread of the Platanus powdery mildew, Erysiphe platani, has been carried out. E. platani teleomorph formation was recorded in countries where the fungus anamorph has been present for several years. The first findings of chasmothecia were recorded in Austria, Czech Republic, France, Italy and Slovakia. New records of E. platani (including the teleomorph) were found in Belgium, Croatia and Denmark. The occurrence of this fungus in Sweden and in two countries of North Africa (Algeria and Morocco) was confirmed. Descriptions of morphological features, illustrations, and worldwide distribution of E. platani are provided. Herbarium collections of powdery mildews on Platanus spp. were re-examined and revised. The occurrence of Phyllactinia guttata on Platanus is discussed and questioned. |
Ondrušková, E; Juhásová, G; Pastirčáková, K The lily magnolia powdery mildew Erysiphe magnifica found in Slovakia Journal Article Mycotaxon, 127 , pp. 51-57, 2014, ISSN: 0093-4666. @article{E.2014, title = {The lily magnolia powdery mildew \textit{Erysiphe magnifica} found in Slovakia}, author = {E. Ondrušková and G. Juhásová and K. Pastirčáková}, url = {http://www.ingentaconnect.com/contentone/mtax/mt/2014/00000127/00000001/art00010}, doi = {10.5248/127.51}, issn = {0093-4666}, year = {2014}, date = {2014-03-01}, journal = {Mycotaxon}, volume = {127}, pages = {51-57}, abstract = {Powdery mildew symptoms were observed on leaves of lily magnolia (Magnolia liliiflora) in Slovakia. The causal fungus was identified as Erysiphe magnifica based on the morphology of both anamorphic and teleomorphic stages. This is the first report of E. magnifica on lily magnolia in Slovakia. A detailed description, illustrations, the host range, and the distribution of this fungus are given.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Powdery mildew symptoms were observed on leaves of lily magnolia (Magnolia liliiflora) in Slovakia. The causal fungus was identified as Erysiphe magnifica based on the morphology of both anamorphic and teleomorphic stages. This is the first report of E. magnifica on lily magnolia in Slovakia. A detailed description, illustrations, the host range, and the distribution of this fungus are given. |
Zúbrik, M; Barta, M; Pilarska, D; Goertz, D; Úradník, M; Galko, J; Vakula, J; Gubka, A; Rella, S; Kunca, A First record of Entomophaga maimaiga (Entomophthorales: Entomophthoraceae) in Slovakia Journal Article Biocontrol Science and Technology, 24 (6), pp. 710-714, 2014, ISSN: 1360-0478. @article{Zúbrik2014, title = {First record of \textit{Entomophaga maimaiga} (Entomophthorales: Entomophthoraceae) in Slovakia}, author = {M. Zúbrik and M. Barta and D. Pilarska and D. Goertz and M. Úradník and J. Galko and J. Vakula and A. Gubka and S. Rella and A. Kunca}, url = {http://www.tandfonline.com/doi/full/10.1080/09583157.2014.883362?scroll=top&needAccess=true}, doi = {http://dx.doi.org/10.1080/09583157.2014.883362}, issn = {1360-0478}, year = {2014}, date = {2014-01-07}, journal = {Biocontrol Science and Technology}, volume = {24}, number = {6}, pages = {710-714}, abstract = {The entomopathogenic fungus Entomophaga maimaiga was found for the first time in Slovakia in 2013. Late instar larvae of gypsy moth, Lymantria dispar, from two sites with different population densities were dissected to evaluate the presence of pathogens. The presence of conidia and resting spores of E. maimaiga in gypsy moth cadavers was confirmed from both sites.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The entomopathogenic fungus Entomophaga maimaiga was found for the first time in Slovakia in 2013. Late instar larvae of gypsy moth, Lymantria dispar, from two sites with different population densities were dissected to evaluate the presence of pathogens. The presence of conidia and resting spores of E. maimaiga in gypsy moth cadavers was confirmed from both sites. |
Balkovič, J; Bedrna, Z; Bublinec, E; Čurlík, J; Dlapa, P; Fulajtár, E; Gömöryová, E; Gregor, J; Hanes, J; Houšková, B; Hutár, V; Chlpík, J; Ilavská, B; Juráni, B; Kobza, B; Kotorková, D; Kukla, J; Kuklová, M; Machava, J; Nádašský, J; Nochta, P; Nováková, K; Orfánus, T; Pavlenda, P; Pichler, V; Pišút, P; Skalský, R; Sobocká, J; Šimanský, V; Tatarková, Z; Tobiášová, E; Zaujec, A; Zverková, M Morphogenetic soil classification system of Slovakia: basal reference taxonomy Book 2nd edition, Societas pedologica slovaca, Bratislava, 2014, ISBN: 978-80-8163-005-7. @book{Balkovič2014, title = {Morphogenetic soil classification system of Slovakia: basal reference taxonomy}, author = {J. Balkovič and Z. Bedrna and E. Bublinec and J. Čurlík and P. Dlapa and E. Fulajtár and E. Gömöryová and J. Gregor and J. Hanes and B. Houšková and V. Hutár and J. Chlpík and B. Ilavská and B. Juráni and B. Kobza and D. Kotorková and J. Kukla and M. Kuklová and J. Machava and J. Nádašský and P. Nochta and K. Nováková and T. Orfánus and P. Pavlenda and V. Pichler and P. Pišút and R. Skalský and J. Sobocká and V. Šimanský and Z. Tatarková and E. Tobiášová and A. Zaujec and M. Zverková}, isbn = {978-80-8163-005-7}, year = {2014}, date = {2014-01-01}, pages = {96}, publisher = {Societas pedologica slovaca}, address = {Bratislava}, edition = {2nd edition}, institution = {Výskumný ústav pôdoznalectva a ochrany pôdy}, keywords = {}, pubstate = {published}, tppubtype = {book} } |
Juhásová, G; Adamčíková, K; Kobza, M; Ostrovský, R Príčiny hromadného hynutia gaštana jedlého [Causal agent of European chestnut blight] Journal Article Zahradnictví : časopis profesionálních zahradníků, 13 (7), pp. 48-51, 2014, ISSN: 1213-7596. @article{Juhásová2014, title = {Príčiny hromadného hynutia gaštana jedlého [Causal agent of European chestnut blight]}, author = {G. Juhásová and K. Adamčíková and M. Kobza and R. Ostrovský}, issn = {1213-7596}, year = {2014}, date = {2014-01-01}, journal = {Zahradnictví : časopis profesionálních zahradníků}, volume = {13}, number = {7}, pages = {48-51}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Bolvanský, M; Ostrovský, R; Kobza, M; Adamčíková, K; Pažitný, J; Juhásová, G; Kajaba, P Spread of chestnut blight in Slovakia in relation to the site topography and climatic characteristics Inproceedings Acta Horticulturae: Proceedings of the second European congress on chestnut : Debrecen, Hungary, Baia Mare, Romania, Modry Kamen, Slovakia, October 9-12, 2013. , pp. 35-42, ISHS, 2014, ISBN: 978 94 6261 032 3. @inproceedings{Bolvanský2014, title = {Spread of chestnut blight in Slovakia in relation to the site topography and climatic characteristics}, author = {M. Bolvanský and R. Ostrovský and M. Kobza and K. Adamčíková and J. Pažitný and G. Juhásová and P. Kajaba}, isbn = {978 94 6261 032 3}, year = {2014}, date = {2014-01-01}, booktitle = {Acta Horticulturae: Proceedings of the second European congress on chestnut : Debrecen, Hungary, Baia Mare, Romania, Modry Kamen, Slovakia, October 9-12, 2013. }, number = {1043}, pages = {35-42}, publisher = {ISHS}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
Adamčíková, K; Kobza, M; Juhásová, G; Ondrušková, E; Bolvanský, M; Kádasi-Horáková, M Gaštan jedlý na Slovensku a v Európe : pestovanie, ochrana, variabilita a využitie [European chestnut in Slovakia : growing, protection, use and genetic variability] Book Garamond, Nitra, 2014, ISBN: 978-80-89408-18-4. @book{Adamčíková2014, title = {Gaštan jedlý na Slovensku a v Európe : pestovanie, ochrana, variabilita a využitie [European chestnut in Slovakia : growing, protection, use and genetic variability]}, author = {K. Adamčíková and M. Kobza and G. Juhásová and E. Ondrušková and M. Bolvanský and M. Kádasi-Horáková}, isbn = {978-80-89408-18-4}, year = {2014}, date = {2014-01-01}, volume = {1}, pages = {155}, publisher = {Garamond}, address = {Nitra}, keywords = {}, pubstate = {published}, tppubtype = {book} } |
Ivanová, H Birch necrotic leaf spots caused by fungal pathogens Journal Article Folia Oecologica, 41 (2), 2014. @article{Ivanová2014, title = {Birch necrotic leaf spots caused by fungal pathogens}, author = {H. Ivanová}, year = {2014}, date = {2014-01-01}, journal = {Folia Oecologica}, volume = {41}, number = {2}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H Birch necrotic leaf spots caused by fungal pathogens Journal Article Folia Oecologica, 41 (2), pp. 134-140, 2014, ISSN: 1336-5266. @article{Ivanová2014b, title = {Birch necrotic leaf spots caused by fungal pathogens}, author = {H. Ivanová}, url = {http://ife.sk/wp-content/uploads/2016/10/FO_v41_iss2_134to140.pdf}, issn = {1336-5266}, year = {2014}, date = {2014-01-01}, journal = {Folia Oecologica}, volume = {41}, number = {2}, pages = {134-140}, abstract = {Microscopic fungi associated with lesions on the leaves of Betula pendula Roth. species growing in different greenery types of Nitra town are causal factors weakening the health state and vitality of these trees. Many of them caused anthracnose and formed small, irregular, circular brown spots with dark brown margins or broad necrotic blotches. Disease symptoms begin as a large spots or blotches, which rapidly widen and join, resulting in large areas of dead tissue. The fungi overwinter on infected fallen leaves. Sporulation begins when spring conditions become warm and wet. During the study of the mycoflora of birch leaves ten fungal species were recorded: Asteroma microspermum, Cryptocline betularum, Discula betulae, Marssonina betulae, Melanconium betulinum, Phoma sp., Phomopsis sp., Phyllosticta betulina, Pyrenopeziza betulicola, Stemphylium sp. In this work were described disease symptoms and some distinctive morphological features.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Microscopic fungi associated with lesions on the leaves of Betula pendula Roth. species growing in different greenery types of Nitra town are causal factors weakening the health state and vitality of these trees. Many of them caused anthracnose and formed small, irregular, circular brown spots with dark brown margins or broad necrotic blotches. Disease symptoms begin as a large spots or blotches, which rapidly widen and join, resulting in large areas of dead tissue. The fungi overwinter on infected fallen leaves. Sporulation begins when spring conditions become warm and wet. During the study of the mycoflora of birch leaves ten fungal species were recorded: Asteroma microspermum, Cryptocline betularum, Discula betulae, Marssonina betulae, Melanconium betulinum, Phoma sp., Phomopsis sp., Phyllosticta betulina, Pyrenopeziza betulicola, Stemphylium sp. In this work were described disease symptoms and some distinctive morphological features. |
Bolvanský, M; Adamčíková, K; Kobza, M Screening resistance to chestnut blight in young chestnut trees derived from Castanea sativa × C. crenata hybrids Journal Article Folia Oecologica, 41 (1), pp. 1-7, 2014, ISSN: 1336-5266. @article{Bolvanský2014c, title = {Screening resistance to chestnut blight in young chestnut trees derived from \textit{Castanea sativa} × \textit{C. crenata} hybrids}, author = {M. Bolvanský and K. Adamčíková and M. Kobza}, url = {http://ife.sk/wp-content/uploads/2016/10/FO_v41_iss1_1to7.pdf}, issn = {1336-5266}, year = {2014}, date = {2014-01-01}, journal = {Folia Oecologica}, volume = {41}, number = {1}, pages = {1-7}, abstract = {In 2010, 2011 and 2012 four trials were carried out to prove a reliability of the new screening method for resistance to chestnut blight caused by Cryphonectria parasitica. In the selected trees of hybrid origin (C. sativa × C. crenata)) 10 cm long cut-branch sections were inoculated with mycelia of three virulent isolates and one hypovirulent isolate (in two last trials) and cultivated for 7 days in the dark at 25 °C with 95% humidity. Then the bark of branch segments was peeled off and the size of necrotic lesions formed on the wood tissue around the inoculation place was measured. The size of necrotic areas varied by sampling date, type of fungus strains and tested trees. In all three summer trials necrotic lesions were larger than lesions in a spring trial of 2011, in which still dormant stem sections were used. Unlike the summer trials in the spring trial higher differences in the size of necrotic lesions among tested trees and among used virulent strains were observed. In the spring trial interactions between tested trees and fungal isolates were not so frequent like in summer trials where more trees exhibited different response to the same virulent strain. Majority of trees showed different susceptibility in particular trials. Observed high variation of reactions of tested trees to both virulent and hypovirulent isolates has pointed at the need to prove other screening methods, and to find such one, that would be highly effective to reveal an inherited resistance and/or a lower degree of susceptibility to chestnut blight.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In 2010, 2011 and 2012 four trials were carried out to prove a reliability of the new screening method for resistance to chestnut blight caused by Cryphonectria parasitica. In the selected trees of hybrid origin (C. sativa × C. crenata)) 10 cm long cut-branch sections were inoculated with mycelia of three virulent isolates and one hypovirulent isolate (in two last trials) and cultivated for 7 days in the dark at 25 °C with 95% humidity. Then the bark of branch segments was peeled off and the size of necrotic lesions formed on the wood tissue around the inoculation place was measured. The size of necrotic areas varied by sampling date, type of fungus strains and tested trees. In all three summer trials necrotic lesions were larger than lesions in a spring trial of 2011, in which still dormant stem sections were used. Unlike the summer trials in the spring trial higher differences in the size of necrotic lesions among tested trees and among used virulent strains were observed. In the spring trial interactions between tested trees and fungal isolates were not so frequent like in summer trials where more trees exhibited different response to the same virulent strain. Majority of trees showed different susceptibility in particular trials. Observed high variation of reactions of tested trees to both virulent and hypovirulent isolates has pointed at the need to prove other screening methods, and to find such one, that would be highly effective to reveal an inherited resistance and/or a lower degree of susceptibility to chestnut blight. |
2013 |
Pastirčáková, K; Pastirčák, M A powdery mildew (Pseudoidium sp.) found on Chelidonium majus in the Czech Republic and Slovakia Journal Article Czech Mycology, 65 (1), pp. 125-133, 2013, ISSN: 1211-0981. @article{K.2013b, title = {A powdery mildew (\textit{Pseudoidium} sp.) found on \textit{Chelidonium majus} in the Czech Republic and Slovakia}, author = {K. Pastirčáková and M. Pastirčák}, url = {http://www.czechmycology.org/_cmo/CM65110.pdf}, issn = {1211-0981}, year = {2013}, date = {2013-06-10}, journal = {Czech Mycology}, volume = {65}, number = {1}, pages = {125-133}, abstract = {The find of a powdery mildew (Pseudoidium sp.) on Chelidonium majus in the Czech Republic and Slovakia is reported. Disease symptoms and morphological characteristics of the anamorph are described. No teleomorph was observed. Morphology and distribution of other powdery mildew species on hosts of the Papaveraceae are discussed. We point out the need for phylogenetic analysis of three closely related species (Erysiphe cruciferarum, E. hylomeci, and E. macleayae) and determination of its relationship to the fungus on C. majus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The find of a powdery mildew (Pseudoidium sp.) on Chelidonium majus in the Czech Republic and Slovakia is reported. Disease symptoms and morphological characteristics of the anamorph are described. No teleomorph was observed. Morphology and distribution of other powdery mildew species on hosts of the Papaveraceae are discussed. We point out the need for phylogenetic analysis of three closely related species (Erysiphe cruciferarum, E. hylomeci, and E. macleayae) and determination of its relationship to the fungus on C. majus. |
Retamal, M; Barta, M; Pérez, E R; Flores, E V Neozygites osornensis sp. nov., a fungal species causing mortality to the cypress aphid Cinara cupressi in Chile Journal Article Mycologia, 105 (3), pp. 661 – 669, 2013, ISSN: 1557-2536. @article{Retamal2013, title = {\textit{Neozygites osornensis} sp. nov., a fungal species causing mortality to the cypress aphid \textit{Cinara cupressi} in Chile}, author = {M. Retamal and M. Barta and E. R. Pérez and E. V. Flores}, url = {http://www.tandfonline.com/doi/full/10.3852/12-125}, doi = {DOI: 10.3852/12-125}, issn = {1557-2536}, year = {2013}, date = {2013-01-20}, journal = {Mycologia}, volume = {105}, number = {3}, pages = {661 – 669}, abstract = {An entomophthoralean fungus causing epizootics in populations of the cypress aphid, \textit{Cinara cupressi} Buckton, in Chile is described as a new species, \textit{Neozygites osornensis} Montalva et Barta. The aphid pathogen is described based on morphological characters. An exhaustive description, illustrations and a comparison with closely related species are provided. The fungus differs from similar \textit{Neozygites} species by smaller hyphal bodies, nuclei, primary conidia, capilliconidia and capilliphores and by noticeably different shape of capilliconidia. A key to aphid-pathogenic species of \textit{Neozygites} is also included.}, keywords = {}, pubstate = {published}, tppubtype = {article} } An entomophthoralean fungus causing epizootics in populations of the cypress aphid, Cinara cupressi Buckton, in Chile is described as a new species, Neozygites osornensis Montalva et Barta. The aphid pathogen is described based on morphological characters. An exhaustive description, illustrations and a comparison with closely related species are provided. The fungus differs from similar Neozygites species by smaller hyphal bodies, nuclei, primary conidia, capilliconidia and capilliphores and by noticeably different shape of capilliconidia. A key to aphid-pathogenic species of Neozygites is also included. |
Ivanová, H; Bernadovičová, S Coniochaeta prunicola – first record for Slovakia and Europe Journal Article Central European Journal of Biology, 8 (2), pp. 195–200, 2013. @article{H.2013, title = {\textit{Coniochaeta prunicola} – first record for Slovakia and Europe}, author = {H. Ivanová and S. Bernadovičová}, url = {http://link.springer.com/article/10.2478/s11535-013-0122-4}, year = {2013}, date = {2013-01-01}, journal = {Central European Journal of Biology}, volume = {8}, number = {2}, pages = {195–200}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Adamčíková, K; Juhásová, G; Kobza, M; Ondrušková, E Diversity of microfungi on branches of Castanea sativa in Slovakia [Diverzita mikroskopických húb na konároch Castanea sativa na Slovensku] Journal Article Polish Botanical Journal, 58 (2), pp. 741-746, 2013, ISSN: 1641-8180. @article{Adamčíková2013, title = {Diversity of microfungi on branches of Castanea sativa in Slovakia [Diverzita mikroskopických húb na konároch Castanea sativa na Slovensku]}, author = {K. Adamčíková and G. Juhásová and M. Kobza and E. Ondrušková}, issn = {1641-8180}, year = {2013}, date = {2013-01-01}, journal = {Polish Botanical Journal}, volume = {58}, number = {2}, pages = {741-746}, abstract = {Localities in Castanea sativa Mill. plantations were visited in a study aimed at identifying the mycoflora of C. sativa in Slovakia [excluding Cryphonectria parasitica (Murr.), Barr for which much data is available]. Samples from chestnut tree branches and stems were examined visually and microscopically. Seven species of microfungi were recorded, three with their anamorphs. Coryneum modonium (Sacc.) Griffon & Maubl. and Phomopsis castaneae Woron. were the most common. Libertella quercina Tul. & C. Tul. was identified in both states (anamorph and teleomorph) at two new localities. The records of Gloniopsis praelonga (Schwein.) Underw. & Earle and Dothidotthia celtidis (Ellis & Everh.) M. E. Barr are the first for Slovakia. These species were rare, found only in one locality. Two microscopic fungi were detected: Valsa ambiens (Pers.) Fr. [Cytospora leucosperma (Pers.) Fr.] and Diplodina castaneae Prill. & Delacr.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Localities in Castanea sativa Mill. plantations were visited in a study aimed at identifying the mycoflora of C. sativa in Slovakia [excluding Cryphonectria parasitica (Murr.), Barr for which much data is available]. Samples from chestnut tree branches and stems were examined visually and microscopically. Seven species of microfungi were recorded, three with their anamorphs. Coryneum modonium (Sacc.) Griffon & Maubl. and Phomopsis castaneae Woron. were the most common. Libertella quercina Tul. & C. Tul. was identified in both states (anamorph and teleomorph) at two new localities. The records of Gloniopsis praelonga (Schwein.) Underw. & Earle and Dothidotthia celtidis (Ellis & Everh.) M. E. Barr are the first for Slovakia. These species were rare, found only in one locality. Two microscopic fungi were detected: Valsa ambiens (Pers.) Fr. [Cytospora leucosperma (Pers.) Fr.] and Diplodina castaneae Prill. & Delacr. |
Ivanová, H Anatomic-morphological characteristic of fungus Coniochaeta prunicola isolated from Prunus cerasus leaves Journal Article Folia Oecologica, 40 (1), pp. 28-33, 2013. @article{Ivanová2013, title = {Anatomic-morphological characteristic of fungus Coniochaeta prunicola isolated from Prunus cerasus leaves}, author = {H. Ivanová}, year = {2013}, date = {2013-01-01}, journal = {Folia Oecologica}, volume = {40}, number = {1}, pages = {28-33}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H Coniochaeta prunicola-causal factor involved in health state decline of selected trees of the genus Prunus Journal Article Folia Oecologica, 40 (2), pp. 188-191, 2013. @article{Ivanová2013b, title = {Coniochaeta prunicola-causal factor involved in health state decline of selected trees of the genus Prunus}, author = {H. Ivanová}, year = {2013}, date = {2013-01-01}, journal = {Folia Oecologica}, volume = {40}, number = {2}, pages = {188-191}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2012 |
Ivanová, H; Bernadovičová, S New record of the fungus Coniochaeta prunicola on peaches from Slovakia Journal Article Biologia, 67 (2), pp. 269–273, 2012. @article{H.2012b, title = {New record of the fungus \textit{Coniochaeta prunicola} on peaches from Slovakia}, author = {H. Ivanová and S. Bernadovičová}, url = {http://link.springer.com/article/10.2478/s11756-012-0010-4}, year = {2012}, date = {2012-02-22}, journal = {Biologia}, volume = {67}, number = {2}, pages = {269–273}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Adamčíková, K; Kobza, M; Bolvanský, M; Ondrušková, E Spread and population structure of Cryphonectria parasitica in a young chestnut orchard in Slovakia Journal Article Central European Journal of Biology, 7 (2), pp. 267–274, 2012. @article{K.2012, title = {Spread and population structure of \textit{Cryphonectria parasitica} in a young chestnut orchard in Slovakia}, author = {K. Adamčíková and M. Kobza and M. Bolvanský and E. Ondrušková}, url = {http://link.springer.com/article/10.2478/s11535-012-0009-4}, year = {2012}, date = {2012-02-10}, journal = {Central European Journal of Biology}, volume = {7}, number = {2}, pages = {267–274}, abstract = {The chestnut blight pathogen Cryphonectria parasitica was studied in a chestnut collection composed of both seedlings and grafts derived from selected Castanea sativa and C. sativa x C. crenata trees located in south-east Slovakia, near village Príbelce on an area of approximately 3.5 ha. The study was conducted during eight years (2003-2010). During this period 133 trees were infected, which represents 59.82% of chestnut trees of all chestnut accessions. Based on the phenotype of the fungus culture and the type of cankers in the field, all isolates were determined to be virulent. No hypovirulent strains were found. No vegetative compatibility (vc) type diversity was observed. More than 130 isolates were analyzed for vc and all were in single vc type, which was identical with EU 12. All isolates assayed for mating type were MAT-1. No perithecia were observed. No significant differences were found between the proportion of cankered and dead cankered trees in seedlings and grafts of hybrid origin (C. sativa x C. crenata) and of C. sativa origin. However, particular seedlings and grafts of hybrid origin seemed to exhibit certain resistance to chestnut blight.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The chestnut blight pathogen Cryphonectria parasitica was studied in a chestnut collection composed of both seedlings and grafts derived from selected Castanea sativa and C. sativa x C. crenata trees located in south-east Slovakia, near village Príbelce on an area of approximately 3.5 ha. The study was conducted during eight years (2003-2010). During this period 133 trees were infected, which represents 59.82% of chestnut trees of all chestnut accessions. Based on the phenotype of the fungus culture and the type of cankers in the field, all isolates were determined to be virulent. No hypovirulent strains were found. No vegetative compatibility (vc) type diversity was observed. More than 130 isolates were analyzed for vc and all were in single vc type, which was identical with EU 12. All isolates assayed for mating type were MAT-1. No perithecia were observed. No significant differences were found between the proportion of cankered and dead cankered trees in seedlings and grafts of hybrid origin (C. sativa x C. crenata) and of C. sativa origin. However, particular seedlings and grafts of hybrid origin seemed to exhibit certain resistance to chestnut blight. |
Juhásová, G; Adamčíková, K; Bolvanský, M; Ivanová, H; Tokár, F; Hrubík, P; Konôpková, J; Kobza, M; Ondrušková, M; Kollár, J; Kunová, A Gaštan jedlý na Slovensku. Perspektívy jeho ochrany a pestovania. Book Garmond, Nitra, 2012, ISBN: 978-80-89408-14-6 1. @book{Juhásová2012, title = {Gaštan jedlý na Slovensku. Perspektívy jeho ochrany a pestovania.}, author = {G. Juhásová and K. Adamčíková and M. Bolvanský and H. Ivanová and F. Tokár and P. Hrubík and J. Konôpková and M. Kobza and M. Ondrušková and J. Kollár and A. Kunová}, isbn = {978-80-89408-14-6 1}, year = {2012}, date = {2012-01-01}, pages = {156}, publisher = {Garmond}, address = {Nitra}, keywords = {}, pubstate = {published}, tppubtype = {book} } |
Ivanová, H; Bernardovičová, S New record of the Coniochaeta prunicola on Prunus persica from Slovakia Journal Article Biologia (Bratislava), 67 (2), pp. 269-273, 2012. @article{Ivanová2012, title = {New record of the Coniochaeta prunicola on Prunus persica from Slovakia}, author = {H. Ivanová and S. Bernardovičová}, year = {2012}, date = {2012-01-01}, journal = {Biologia (Bratislava)}, volume = {67}, number = {2}, pages = {269-273}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H; Kaločaiová, M; Bolvanský, M Shot-hole disease on Prunus persica - The morphology and biology of the Stigmina carpophila study Journal Article Folia Oecologica, 39 (1), pp. 21-27, 2012. @article{Ivanová2012b, title = {Shot-hole disease on Prunus persica - The morphology and biology of the Stigmina carpophila study}, author = {H. Ivanová and M. Kaločaiová and M. Bolvanský}, year = {2012}, date = {2012-01-01}, journal = {Folia Oecologica}, volume = {39}, number = {1}, pages = {21-27}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2011 |
Mahmoud, M F; Barta, M Horticultural science (Prague), 38 (2), pp. 54–62, 2011, ISSN: 1805-9333. @article{Mahmoud2011, title = {Effect of gamma radiation on the male sterility and other quality parameters of peach fruit fly, \textit{Bactrocera zonata} (Saunders) (Diptera: Tephritidae)}, author = {M. F. Mahmoud and M. Barta}, url = {http://www.agriculturejournals.cz/publicFiles/39310.pdf}, issn = {1805-9333}, year = {2011}, date = {2011-01-01}, journal = {Horticultural science (Prague)}, volume = {38}, number = {2}, pages = {54–62}, abstract = {Bactrocera zonata, a serious pest of fruits in many parts of the world, has recently been recorded in Northern Africa. Even though it has not been introduced to the European continent yet, a strong emphasis is being placed on developing effective measures to suppress this pest and to prevent it from establishing in neighbouring European countries. The sterile insect technique is widely used in integrated programmes against tephritid fruit flies and, in this paper, quality parameters of irradiated B. zonata were evaluated for possible use of sterile insect technique within the management of this pest. Pupae were irradiated (60Co) 48 h before adult emergence (in an air atmosphere) with doses of 10, 30, 50, 70 or 90 Gy. While adult emergence and egg hatch decreased with increasing dose, no significant differences in female fecundity were found among doses. Exposure of pupae to 90 Gy resulted in a total sterility of eggs laid by non-treated females crossed with treated males. Only insignificant difference in the radiation effect on female fecundity was found. Moderate effects on sex ratio and size were recorded, as they decreased gradually by increasing doses. No considerable effect on flying capability was observed, but generally, the percentage of fliers decreased with increasing radiation doses. Fried’s competitiveness values of treated males (30 and 70 Gy) suggest that irradiated males compete successfully with non-irradiated ones.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Bactrocera zonata, a serious pest of fruits in many parts of the world, has recently been recorded in Northern Africa. Even though it has not been introduced to the European continent yet, a strong emphasis is being placed on developing effective measures to suppress this pest and to prevent it from establishing in neighbouring European countries. The sterile insect technique is widely used in integrated programmes against tephritid fruit flies and, in this paper, quality parameters of irradiated B. zonata were evaluated for possible use of sterile insect technique within the management of this pest. Pupae were irradiated (60Co) 48 h before adult emergence (in an air atmosphere) with doses of 10, 30, 50, 70 or 90 Gy. While adult emergence and egg hatch decreased with increasing dose, no significant differences in female fecundity were found among doses. Exposure of pupae to 90 Gy resulted in a total sterility of eggs laid by non-treated females crossed with treated males. Only insignificant difference in the radiation effect on female fecundity was found. Moderate effects on sex ratio and size were recorded, as they decreased gradually by increasing doses. No considerable effect on flying capability was observed, but generally, the percentage of fliers decreased with increasing radiation doses. Fried’s competitiveness values of treated males (30 and 70 Gy) suggest that irradiated males compete successfully with non-irradiated ones. |
Bernardovičová, S; Ivanová, H Some of Hyphomycetes and Coelomycetes fungi isolated from affected leaves and twigs of cherry laurel trees Journal Article Folia Oecologica, 38 (2), pp. 137-145, 2011. @article{Bernardovičová2011, title = {Some of Hyphomycetes and Coelomycetes fungi isolated from affected leaves and twigs of cherry laurel trees}, author = {S. Bernardovičová and H. Ivanová}, year = {2011}, date = {2011-01-01}, journal = {Folia Oecologica}, volume = {38}, number = {2}, pages = {137-145}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2010 |
Rahman, K M A; Barta, M; Cagáň, Ľ Effects of combining Beauveria bassiana and Nosema pyrausta on the mortality of Ostrinia nubilalis Journal Article Central European Journal of Biology, 5 (4), pp. 472–480, 2010, ISSN: 2391-5412. @article{Rahman2010, title = {Effects of combining \textit{Beauveria bassiana} and \textit{Nosema pyrausta} on the mortality of \textit{Ostrinia nubilalis}}, author = {K. M. A. Rahman and M. Barta and Ľ. Cagáň}, url = {https://www.degruyter.com/view/j/biol.2010.5.issue-4/s11535-010-0035-z/s11535-010-0035-z.xml}, doi = {DOI: 10.2478/s11535-010-0035-z}, issn = {2391-5412}, year = {2010}, date = {2010-08-01}, journal = {Central European Journal of Biology}, volume = {5}, number = {4}, pages = {472–480}, abstract = {We tested the combined effect of the fungus Beauveria bassiana and the microsporidium Nosema pyrausta on the European corn borer larvae, Ostrinia nubilalis, in the laboratory. The first instar of O. nubilalis larvae was the most sensitive to the B. bassiana infection followed by the fifth, second, third, and fourth instar (LC50s were 4.91, 6.67, 7.13, 9.15, and 6.51 x 105 conidia/ml for the first to fifth instars, respectively). Mortality of each instar increases positively with concentration of conidia. When B. bassiana and N. pyrausta were used in combination, mortality increased significantly in all instars. Relative to the B. bassiana treatment alone, the B. bassiana + N. pyrausta treatment decreased the LC50s by 42.16%, 37.63%, 21.60%, 27.11%, and 33.95% for the first to fifth instars, respectively. The combined effects of the two pathogens were mostly additive. However, at the two highest concentrations the pathogens interacted synergistically in the first and second instar. Individuals that survived the B. bassiana and B. bassiana + N. pyrausta treatments and developed into adults had significantly shorter lifespans and females oviposited fewer eggs than non-exposed insects. The effects on the longevity and the egg production were most pronounced at high concentration of B. bassiana conidia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We tested the combined effect of the fungus Beauveria bassiana and the microsporidium Nosema pyrausta on the European corn borer larvae, Ostrinia nubilalis, in the laboratory. The first instar of O. nubilalis larvae was the most sensitive to the B. bassiana infection followed by the fifth, second, third, and fourth instar (LC50s were 4.91, 6.67, 7.13, 9.15, and 6.51 x 105 conidia/ml for the first to fifth instars, respectively). Mortality of each instar increases positively with concentration of conidia. When B. bassiana and N. pyrausta were used in combination, mortality increased significantly in all instars. Relative to the B. bassiana treatment alone, the B. bassiana + N. pyrausta treatment decreased the LC50s by 42.16%, 37.63%, 21.60%, 27.11%, and 33.95% for the first to fifth instars, respectively. The combined effects of the two pathogens were mostly additive. However, at the two highest concentrations the pathogens interacted synergistically in the first and second instar. Individuals that survived the B. bassiana and B. bassiana + N. pyrausta treatments and developed into adults had significantly shorter lifespans and females oviposited fewer eggs than non-exposed insects. The effects on the longevity and the egg production were most pronounced at high concentration of B. bassiana conidia. |
Shoaib, M A; Mahmoud, M F; Loutfy, N; Tawfic, M A; Barta, M Effect of botanical insecticide Nimbecidine® on food consumption and egg hatchability of the terrestrial snail Monacha obstructa Journal Article Journal of Pest Science, 83 (1), pp. 27–32, 2010, ISSN: 1612-4758. @article{Shoaib2010, title = {Effect of botanical insecticide Nimbecidine® on food consumption and egg hatchability of the terrestrial snail \textit{Monacha obstructa}}, author = {M. A. Shoaib and M. F. Mahmoud and N. Loutfy and M. A. Tawfic and M. Barta}, url = {https://link.springer.com/article/10.1007%2Fs10340-009-0265-x?LI=true}, doi = {doi:10.1007/s10340-009-0265-x}, issn = {1612-4758}, year = {2010}, date = {2010-02-01}, journal = {Journal of Pest Science}, volume = {83}, number = {1}, pages = {27–32}, abstract = {In the laboratory, a commercial neem-based insecticide—Nimbecidine®—was evaluated as a potential pest management tool for the terrestrial snail, \textit{Monacha obstructa} (Pfeiffer, 1842) (\textit{Hygromiidae}). Effects of different concentrations of the botanical insecticide on food consumption and egg hatchability of the terrestrial snail were studied. Generally, food consumption of immature and adult snails decreased as the concentrations of Nimbecidine® increased. At the highest concentration (10 ml/l), the snails avoided contacting with food completely. The food intake of immature individuals was significantly (p < 0.05) more affected by the Nimbecidine® treatment (at 1.25 ml/l) than that of adults. LC50 of Nimbecidine® for the treated eggs was 2.18 ml/l, and eggs failed to hatch at concentration of 10 ml/l. Nimbecidine® showed sufficient biological activity against the food consumption and eggs viability of \textit{M. obstructa}, thus the preparation has a potential to protect field crops from this pest snails.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the laboratory, a commercial neem-based insecticide—Nimbecidine®—was evaluated as a potential pest management tool for the terrestrial snail, Monacha obstructa (Pfeiffer, 1842) (Hygromiidae). Effects of different concentrations of the botanical insecticide on food consumption and egg hatchability of the terrestrial snail were studied. Generally, food consumption of immature and adult snails decreased as the concentrations of Nimbecidine® increased. At the highest concentration (10 ml/l), the snails avoided contacting with food completely. The food intake of immature individuals was significantly (p < 0.05) more affected by the Nimbecidine® treatment (at 1.25 ml/l) than that of adults. LC50 of Nimbecidine® for the treated eggs was 2.18 ml/l, and eggs failed to hatch at concentration of 10 ml/l. Nimbecidine® showed sufficient biological activity against the food consumption and eggs viability of M. obstructa, thus the preparation has a potential to protect field crops from this pest snails. |
Pastirčáková, K; Pastirčák, M Two important ascomycetes and their anamorphs on twigs of Betula pendula in Slovakia Journal Article Polish Botanical Journal, 55 (2), pp. 373-380, 2010, ISSN: 1641-8180. @article{Pastirčáková2010, title = {Two important ascomycetes and their anamorphs on twigs of \emph{Betula pendula} in Slovakia}, author = {K. Pastirčáková and M. Pastirčák}, issn = {1641-8180}, year = {2010}, date = {2010-01-01}, journal = {Polish Botanical Journal}, volume = {55}, number = {2}, pages = {373-380}, abstract = {The paper reports two fungal species of Pleomassariaceae new for the Slovak mycobiota: Pleomassaria siparia (Berk. & Broome) Sacc. and Splanchnonema argus (Berk. & Broome) Kuntze, found on dead twigs of Betula pendula Roth. The morphology of the fungi and their coelomycetous anamorphs is described, with photographic documentation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The paper reports two fungal species of Pleomassariaceae new for the Slovak mycobiota: Pleomassaria siparia (Berk. & Broome) Sacc. and Splanchnonema argus (Berk. & Broome) Kuntze, found on dead twigs of Betula pendula Roth. The morphology of the fungi and their coelomycetous anamorphs is described, with photographic documentation. |
Ivanová, H; Bernardovičová, S; Pastirčáková, K Influence of selected climatological characteristics on antracnose diseases development on plane trees Journal Article Ekológia (Bratislava), 29 (4), pp. 430-440, 2010. @article{Ivanová2010, title = {Influence of selected climatological characteristics on antracnose diseases development on plane trees}, author = {H. Ivanová and S. Bernardovičová and K. Pastirčáková}, year = {2010}, date = {2010-01-01}, journal = {Ekológia (Bratislava)}, volume = {29}, number = {4}, pages = {430-440}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2009 |
Pastirčáková, K; Pastirčák, M; Celar, F; Shin, H D Guignardia aesculi on species of Aesculus: new records from Europe and Asia Journal Article Mycotaxon, 108 , pp. 287-296, 2009, ISSN: 0093-4666. @article{Pastirčáková2009, title = {\textit{Guignardia aesculi} on species of \textit{Aesculus}: new records from Europe and Asia}, author = {K. Pastirčáková and M. Pastirčák and F. Celar and H.D. Shin}, issn = {0093-4666}, year = {2009}, date = {2009-01-01}, journal = {Mycotaxon}, volume = {108}, pages = {287-296}, abstract = {New localities of Guignardia aesculi on leaves of seven Aesculus species (×carnea, flava, hippocastanum, ×neglecta, parviflora, pavia, turbinata) were recorded in Europe. The teleomorph was found on overwintered leaves of A. hippocastanum in Slovakia. The occurrence of the Guignardia leaf blotch on A. hippocastanum and A. turbinata was also confirmed for the first time in South Korea. The causal fungus Guignardia aesculi and its conidial anamorph Phyllosticta sphaeropsoidea and spermatial synanamorph Leptodothiorella aesculicola are described in detail and illustrated. Pathogenicity of the fungus was confirmed by inoculating horse chestnut leaves with conidia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } New localities of Guignardia aesculi on leaves of seven Aesculus species (×carnea, flava, hippocastanum, ×neglecta, parviflora, pavia, turbinata) were recorded in Europe. The teleomorph was found on overwintered leaves of A. hippocastanum in Slovakia. The occurrence of the Guignardia leaf blotch on A. hippocastanum and A. turbinata was also confirmed for the first time in South Korea. The causal fungus Guignardia aesculi and its conidial anamorph Phyllosticta sphaeropsoidea and spermatial synanamorph Leptodothiorella aesculicola are described in detail and illustrated. Pathogenicity of the fungus was confirmed by inoculating horse chestnut leaves with conidia. |
Adamčíková, K; Kobza, M; Juhasová, G Horticultural Science , 36 (2), pp. 55-60, 2009. @article{Adamcikova2009, title = { The development of population structure of Cryphonectria parasitica on European chestnut (Castanea sativa Mill.) in the Experimental Castanetarium Horne Lefantovce, observed over a 12-year study period}, author = {K. Adamčíková and M. Kobza and G. Juhasová}, url = {http://www.agriculturejournals.cz/publishedArticles/HORTSCI/2009-36-2-55.pdf}, year = {2009}, date = {2009-01-01}, journal = {Horticultural Science }, volume = {36}, number = {2}, pages = {55-60}, abstract = {We studied occurrence of chestnut blight disease in the Experimental Castanetarium Horne Lefantovce, SW Slovakia. The study ran in years 2006-2007 on a set consisting of 889 chestnut trees growing in the clonal orchard on Biological Plot 105. From this number, 857 trees were found healthy without disease symptoms. The chestnut blight disease was identified on 32 of them. The infected trees were examined for presence of pycnidia and perithecia of the causal agent. In all positive cases, the observed morphological characters indicated virulency of the obtained isolates. No hypovirulent isolate was detected on the evaluated experimental plot. In total, six vegetative compatibility (vc) types were specified in the sample consisting of 31 isolates. Our vc types corresponded to the European vc types EU 2, EU 12, EU 13, EU 14, EU 17, EU 19. Two vc types - EU 2 and EU 19 - were dominant. Vc type EU 19 accounted 35.5% and EU 2 32.2% of isolates. The vc type EU 19, which was the most frequent one in the evaluated site, was detected in Slovakia for the first time.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We studied occurrence of chestnut blight disease in the Experimental Castanetarium Horne Lefantovce, SW Slovakia. The study ran in years 2006-2007 on a set consisting of 889 chestnut trees growing in the clonal orchard on Biological Plot 105. From this number, 857 trees were found healthy without disease symptoms. The chestnut blight disease was identified on 32 of them. The infected trees were examined for presence of pycnidia and perithecia of the causal agent. In all positive cases, the observed morphological characters indicated virulency of the obtained isolates. No hypovirulent isolate was detected on the evaluated experimental plot. In total, six vegetative compatibility (vc) types were specified in the sample consisting of 31 isolates. Our vc types corresponded to the European vc types EU 2, EU 12, EU 13, EU 14, EU 17, EU 19. Two vc types - EU 2 and EU 19 - were dominant. Vc type EU 19 accounted 35.5% and EU 2 32.2% of isolates. The vc type EU 19, which was the most frequent one in the evaluated site, was detected in Slovakia for the first time. |
Ivanová, H; Bernardovičová, S Needle disease on English yew caused by the parasitic fungus Cryptocline taxicola (All.) Petr. Journal Article Folia Oecologica, 36 (2), pp. 79-85, 2009. @article{Ivanová2009, title = {Needle disease on English yew caused by the parasitic fungus Cryptocline taxicola (All.) Petr.}, author = {H. Ivanová and S. Bernardovičová}, url = {http://ife.sk/wp-content/uploads/2016/10/FO_36_2_Ivanova.pdf}, year = {2009}, date = {2009-01-01}, journal = {Folia Oecologica}, volume = {36}, number = {2}, pages = {79-85}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H; Bernardovičová, S Species diversity of microscopic fungi on Austrian pines growing in urban greenery of Nitra town Journal Article Folia Oecologica, 37 (2), pp. 168-180, 2009. @article{Ivanová2009b, title = {Species diversity of microscopic fungi on Austrian pines growing in urban greenery of Nitra town}, author = {H. Ivanová and S. Bernardovičová}, url = {http://ife.sk/wp-content/uploads/2016/10/FO_36_2_Ivanova.pdf}, year = {2009}, date = {2009-01-01}, journal = {Folia Oecologica}, volume = {37}, number = {2}, pages = {168-180}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pastirčák, M; Pastirčáková, K European record of Subramaniula thielavioides on opium poppy Journal Article Acta Mycologica, 44 (1), pp. 7-9, 2009, ISSN: 0001-625X. @article{Pastirčák2009, title = {European record of \textit{Subramaniula thielavioides} on opium poppy}, author = {M. Pastirčák and K. Pastirčáková}, issn = {0001-625X}, year = {2009}, date = {2009-01-01}, journal = {Acta Mycologica}, volume = {44}, number = {1}, pages = {7-9}, abstract = {In the course of a study of fungal biodiversity of opium poppy (Papaver somniferum) plants collected in production area of Slovakia, an ascomycete belonging to the genus Subramaniula was isolated. The fungus identified as Subramaniula thielavioides has been reported for the first time from Slovakia. This record also represents the first European locality. Brief morphological description of the fungus based on an isolate from flower petals of opium poppy is provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the course of a study of fungal biodiversity of opium poppy (Papaver somniferum) plants collected in production area of Slovakia, an ascomycete belonging to the genus Subramaniula was isolated. The fungus identified as Subramaniula thielavioides has been reported for the first time from Slovakia. This record also represents the first European locality. Brief morphological description of the fungus based on an isolate from flower petals of opium poppy is provided. |
2008 |
Pastirčáková, K; Takamatsu, S; Shiroya, Y; Pastirčák, M European hornbeam powdery mildew Erysiphe arcuata in Slovakia Journal Article Journal of Phytopathology, 156 (10), pp. 597-601, 2008, ISSN: 0931-1785. @article{pastirčákovápastirčáková2008, title = {European hornbeam powdery mildew \textit{Erysiphe arcuata} in Slovakia}, author = {K. Pastirčáková and S. Takamatsu and Y. Shiroya and M. Pastirčák}, doi = {10.1111/j.1439-0434.2008.01408.x}, issn = {0931-1785}, year = {2008}, date = {2008-01-01}, journal = {Journal of Phytopathology}, volume = {156}, number = {10}, pages = {597-601}, abstract = {Erysiphe arcuata found on European hornbeam (Carpinus betulus) is reported from Slovakia for the first time. Taxonomic characters of the anamorphic and teleomorphic states of E. arcuata were described and illustrated. Additionally, disease symptoms caused by this pathogen were presented. The identification of the fungus is based on morphological data combined with the results of a sequence analysis.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Erysiphe arcuata found on European hornbeam (Carpinus betulus) is reported from Slovakia for the first time. Taxonomic characters of the anamorphic and teleomorphic states of E. arcuata were described and illustrated. Additionally, disease symptoms caused by this pathogen were presented. The identification of the fungus is based on morphological data combined with the results of a sequence analysis. |
Bernardovičová, S; Ivanová, H Occurrence of lindenleaf spots and cultural characteristics of its agent–fungus Cercospora microsora Sacc. Journal Article Biológia, 63 (1), pp. 44-49, 2008. @article{Bernardovičová2008, title = {Occurrence of lindenleaf spots and cultural characteristics of its agent–fungus Cercospora microsora Sacc.}, author = {S. Bernardovičová and H. Ivanová}, year = {2008}, date = {2008-01-01}, journal = {Biológia}, volume = {63}, number = {1}, pages = {44-49}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H Cell wall regeneration of protoplasts isolated from Norvay spruce tissue cultures in a liquid nutrient medium Journal Article Folia Oecologica, 35 (2), pp. 18-24, 2008. @article{Ivanová2008, title = {Cell wall regeneration of protoplasts isolated from Norvay spruce tissue cultures in a liquid nutrient medium}, author = {H. Ivanová}, url = {http://ife.sk/wp-content/uploads/2016/10/FO_35_2_ivanova.pdf}, year = {2008}, date = {2008-01-01}, journal = {Folia Oecologica}, volume = {35}, number = {2}, pages = {18-24}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ivanová, H; Bernardovičová, S Growth variability of Apiognomonia errabunda (Rob. & Desm.) Höhn. isolated from Tilia cordata Mill. Journal Article Acta Fytotechnica et Zootechnica, 11 (3), pp. 64-69, 2008. @article{Ivanová2008b, title = {Growth variability of Apiognomonia errabunda (Rob. & Desm.) Höhn. isolated from Tilia cordata Mill.}, author = {H. Ivanová and S. Bernardovičová}, year = {2008}, date = {2008-01-01}, journal = {Acta Fytotechnica et Zootechnica}, volume = {11}, number = {3}, pages = {64-69}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pastirčáková, K; Ivanová, H; Bernadovičová, S Neoerysiphe galeopsidis on Stachys species in Slovakia and the Czech Republic based on a re-examination of herbarium collections. Journal Article Czech Mycology, 60 (2), pp. 251-264, 2008, ISSN: 1211-0981. @article{Pastirčáková2008b, title = {\textit{Neoerysiphe galeopsidis} on \textit{Stachys} species in Slovakia and the Czech Republic based on a re-examination of herbarium collections.}, author = {K. Pastirčáková and H. Ivanová and S. Bernadovičová}, issn = {1211-0981}, year = {2008}, date = {2008-01-01}, journal = {Czech Mycology}, volume = {60}, number = {2}, pages = {251-264}, abstract = {On the basis of re-examinations of herbarium collections, species of the genus Stachys as host plants of the powdery mildew species Neoerysiphe galeopsidis in Slovakia and the Czech Republic were verified. A detailed description, illustrations, the host range and the distribution of this fungus are given.}, keywords = {}, pubstate = {published}, tppubtype = {article} } On the basis of re-examinations of herbarium collections, species of the genus Stachys as host plants of the powdery mildew species Neoerysiphe galeopsidis in Slovakia and the Czech Republic were verified. A detailed description, illustrations, the host range and the distribution of this fungus are given. |
2007 |
Pastirčák, M; Pastirčáková, K Scopinella solani on graminicolous hosts in Slovakia and the Czech Republic Journal Article Mycotaxon, 102 , pp. 383-387, 2007, ISSN: 0093-4666. @article{Pastirčák2007, title = {\textit{Scopinella solani} on graminicolous hosts in Slovakia and the Czech Republic}, author = {M. Pastirčák and K. Pastirčáková}, issn = {0093-4666}, year = {2007}, date = {2007-01-01}, journal = {Mycotaxon}, volume = {102}, pages = {383-387}, abstract = {Scopinella solani was found on overwintered inflorescences of Brachypodium pinnatum, Dactylis glomerata, and Elytrigia repens. The morphological characteristics and host range of S. solani are described. These are the first records of S. solani in Slovakia and the Czech Republic.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Scopinella solani was found on overwintered inflorescences of Brachypodium pinnatum, Dactylis glomerata, and Elytrigia repens. The morphological characteristics and host range of S. solani are described. These are the first records of S. solani in Slovakia and the Czech Republic. |
Ivanova, H; Bernardovičová, S; Pastirčáková, K Influence of changed ecological conditions of environment on occurrence of London plane (Platanus x hispanica Münchh.) anthracnose Journal Article Folia Oecologica, 34 (1), pp. 1-8, 2007, ISBN: 1336-5266. @article{Ivanova2007, title = {Influence of changed ecological conditions of environment on occurrence of London plane (Platanus x hispanica Münchh.) anthracnose}, author = {H. Ivanova and S. Bernardovičová and K. Pastirčáková}, url = {http://ife.sk/wp-content/uploads/2016/10/FO_34_1_Pastircakova.pdf}, isbn = {1336-5266}, year = {2007}, date = {2007-01-01}, journal = {Folia Oecologica}, volume = {34}, number = {1}, pages = {1-8}, abstract = {During 2004–2007, reappearances of anthracnose on Platanus × hispanica caused by the microscopical fungus Apiognomonia veneta were recorded. Causal agent of the disease was isolated from symptomatic leaves and twigs, with characteristic spots and lesions, sampled from affected host trees growing in urban environment at the selected locality (Nitra). Subsequent identification and morphological description of fungal isolates was made by microscopical differentiation, according to the fungi identification key. Our study has confirmed interannual changes in the disease severity and influence of temperature in period decisive for occurrence and progress of the disease. Cool springs are more promoting severe plane anthrac-nose outbreaks. Repeated annual removal of twigs and leaves results in weakening of plane trees.}, keywords = {}, pubstate = {published}, tppubtype = {article} } During 2004–2007, reappearances of anthracnose on Platanus × hispanica caused by the microscopical fungus Apiognomonia veneta were recorded. Causal agent of the disease was isolated from symptomatic leaves and twigs, with characteristic spots and lesions, sampled from affected host trees growing in urban environment at the selected locality (Nitra). Subsequent identification and morphological description of fungal isolates was made by microscopical differentiation, according to the fungi identification key. Our study has confirmed interannual changes in the disease severity and influence of temperature in period decisive for occurrence and progress of the disease. Cool springs are more promoting severe plane anthrac-nose outbreaks. Repeated annual removal of twigs and leaves results in weakening of plane trees. |
2006 |
Pastirčáková, K; Pastirčák, M The anamorph of Erysiphe platani on Platanus ×hispanica in Slovakia Journal Article Mycotaxon, 97 , pp. 189-194, 2006, ISSN: 0093-4666. @article{Pastirčáková2006, title = {The anamorph of \textit{Erysiphe platani} on \textit{Platanus ×hispanica} in Slovakia}, author = {K. Pastirčáková and M. Pastirčák}, issn = {0093-4666}, year = {2006}, date = {2006-01-01}, journal = {Mycotaxon}, volume = {97}, pages = {189-194}, abstract = {A species of powdery mildew new for the Slovak mycoflora, Erysiphe platani, which parasitizes on plane trees, is reported. Disease symptoms and morphological characteristics of the anamorph are described. No teleomorph was observed. }, keywords = {}, pubstate = {published}, tppubtype = {article} } A species of powdery mildew new for the Slovak mycoflora, Erysiphe platani, which parasitizes on plane trees, is reported. Disease symptoms and morphological characteristics of the anamorph are described. No teleomorph was observed. |
Pastirčáková, K; Pastirčák, M; Juhásová, G The Catalpa powdery mildew Erysiphe elevata in Slovakia Journal Article Cryptogamie, Mycologie, 27 (1), pp. 31-34, 2006, ISSN: 0181-1584. @article{Pastirčáková2006b, title = {The Catalpa powdery mildew \textit{Erysiphe elevata} in Slovakia}, author = {K. Pastirčáková and M. Pastirčák and G. Juhásová}, issn = {0181-1584}, year = {2006}, date = {2006-01-01}, journal = {Cryptogamie, Mycologie}, volume = {27}, number = {1}, pages = {31-34}, abstract = {The first record of Erysiphe elevata (Burrill) U. Braun & S. Takamatsu (syn. Microsphaera elevata Burrill), North American powdery mildew in Slovakia is presented. It was found on living leaves and fruits of southem catalpa (Catalpa bignonioides Walt.). The fungus is described and photographically documented.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The first record of Erysiphe elevata (Burrill) U. Braun & S. Takamatsu (syn. Microsphaera elevata Burrill), North American powdery mildew in Slovakia is presented. It was found on living leaves and fruits of southem catalpa (Catalpa bignonioides Walt.). The fungus is described and photographically documented. |
Ivanová, H; Bernardovičová, S Leaf spots on lindens caused by fungi Cercospora microsora Sacc. and Apiognomonia tiliae (Rehm.) Höhn Journal Article Folia Oecologica, 33 (1), pp. 24-33, 2006. @article{Ivanová2006, title = {Leaf spots on lindens caused by fungi Cercospora microsora Sacc. and Apiognomonia tiliae (Rehm.) Höhn}, author = {H. Ivanová and S. Bernardovičová}, year = {2006}, date = {2006-01-01}, journal = {Folia Oecologica}, volume = {33}, number = {1}, pages = {24-33}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2004 |
Ale-Agha, N; Bolay, A; Braun, U; Feige, B; Jage, H; Kummer, V; Lebeda, A; Piątek, M; Shin, H D; Zimmermannová-Pastirčáková, K Erysiphe catalpae and Erysiphe elevata in Europe Journal Article Mycological Progress, 3 (4), pp. 291-296, 2004, ISSN: 1617-416X. @article{Ale-Agha2004, title = {\textit{Erysiphe catalpae} and \textit{Erysiphe elevata} in Europe}, author = {N. Ale-Agha and A. Bolay and U. Braun and B. Feige and H. Jage and V. Kummer and A. Lebeda and M. Piątek and H.D. Shin and K. Zimmermannová-Pastirčáková}, issn = {1617-416X}, year = {2004}, date = {2004-01-01}, journal = {Mycological Progress}, volume = {3}, number = {4}, pages = {291-296}, abstract = {The recent epidemic spread of the North American powdery mildew Erysiphe elevata in Europe is described and discussed. Since 2002, this plant pathogenic fungus has been collected on Catalpa bignonioides, C. erubescens and C. speciosa in the Czech Republic, Germany, Hungary, Slovakia and Switzerland. The diagnostically important anamorph of E. elevata, so far unknown, is described and illustrated in detail. Type material of Erysiphe catalpae and two specimens of E. catalpae recently collected in Poland have been examined and compared with E. elevata. The anamorph as well as the teleomorph of E. catalpae proved to be easily distinguishable from E. elevata. The supposition that E. catalpae, introduced in Armenia, was based on immature ascomata of E. elevata proved to be wrong. The origin and distribution of E. catalpae are discussed, and a key to powdery mildew fungi on Catalpa spp. in Europe is provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The recent epidemic spread of the North American powdery mildew Erysiphe elevata in Europe is described and discussed. Since 2002, this plant pathogenic fungus has been collected on Catalpa bignonioides, C. erubescens and C. speciosa in the Czech Republic, Germany, Hungary, Slovakia and Switzerland. The diagnostically important anamorph of E. elevata, so far unknown, is described and illustrated in detail. Type material of Erysiphe catalpae and two specimens of E. catalpae recently collected in Poland have been examined and compared with E. elevata. The anamorph as well as the teleomorph of E. catalpae proved to be easily distinguishable from E. elevata. The supposition that E. catalpae, introduced in Armenia, was based on immature ascomata of E. elevata proved to be wrong. The origin and distribution of E. catalpae are discussed, and a key to powdery mildew fungi on Catalpa spp. in Europe is provided. |
2002 |
Zimmermannová-Pastirčáková, K; Pastirčák, M Erysiphe flexuosa – a new species of powdery mildew for Slovakia Journal Article Biologia (Bratislava), 57 (4), pp. 437-440, 2002, ISSN: 0006-3088. @article{Zimmermanová-Pastirčáková2002, title = {\textit{Erysiphe flexuosa} – a new species of powdery mildew for Slovakia}, author = {K. Zimmermannová-Pastirčáková and M. Pastirčák}, issn = {0006-3088}, year = {2002}, date = {2002-01-01}, journal = {Biologia (Bratislava)}, volume = {57}, number = {4}, pages = {437-440}, abstract = {Erysiphe flexuosa (Peck) U. Braun & S. Takamatsu, a new powdery mildew for Slovakia is presented. It was found on living leaves of common horse chestnut (Aesculus hippocastanum L.) and red horse chestnut (Aesculus x camea Hayne). We recorded massive occurrence or this fungus also in Austria. The fungus is described and photographically documented.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Erysiphe flexuosa (Peck) U. Braun & S. Takamatsu, a new powdery mildew for Slovakia is presented. It was found on living leaves of common horse chestnut (Aesculus hippocastanum L.) and red horse chestnut (Aesculus x camea Hayne). We recorded massive occurrence or this fungus also in Austria. The fungus is described and photographically documented. |
Zimmermannová-Pastirčáková, K; Adamska, I; Błaszkowski, J; Bolay, A; Braun, U Epidemic spread of Erysiphe flexuosa (North American powdery mildew disease of horse-chestnut) in Europe Journal Article Schlechtendalia, 8 , pp. 39-45, 2002, ISSN: 1436-2317. @article{Zimmermannová-Pastirčáková2002, title = {Epidemic spread of \textit{Erysiphe flexuosa} (North American powdery mildew disease of horse-chestnut) in Europe}, author = {K. Zimmermannová-Pastirčáková and I. Adamska and J. Błaszkowski and A. Bolay and U. Braun}, issn = {1436-2317}, year = {2002}, date = {2002-01-01}, journal = {Schlechtendalia}, volume = {8}, pages = {39-45}, abstract = {The European epidemic spread of Erysiphe (Uncinula) flexuosa, the North American horse-chestnut powdery mildew fungus, is discussed. New collections of this plant disease from Austria, Croatia, Czech Republik, France, Poland, Slovakia and Switzerland on leaves and occasionally stems of Aesculus × carnea, A. chinensis, A. hippocastanum, A. indica, A. neglecta and A. × plantierensis from 2000 and 2001 are reported. The taxonomy of this fungus is discussed and disease symptoms and microscopic features, including some new observations, are described and illustrated.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The European epidemic spread of Erysiphe (Uncinula) flexuosa, the North American horse-chestnut powdery mildew fungus, is discussed. New collections of this plant disease from Austria, Croatia, Czech Republik, France, Poland, Slovakia and Switzerland on leaves and occasionally stems of Aesculus × carnea, A. chinensis, A. hippocastanum, A. indica, A. neglecta and A. × plantierensis from 2000 and 2001 are reported. The taxonomy of this fungus is discussed and disease symptoms and microscopic features, including some new observations, are described and illustrated. |
2024 |
Fungi associated with ambrosia beetle Xylosandrus germanus in Slovakia Journal Article Biologia, 79 (8), pp. 2387-2400, 2024, ISSN: 0006-3088. |
Taxonomic reintroduction of the holarctic saprotrophic fungus Crepidotus cinnamomeus Journal Article Mycological Progress, 23 , pp. 49, 2024, ISSN: 1617-416X. |
Contributions to the knowledge of the phylogeny and taxonomy of the Erysiphaceae (powdery mildews) – part 2 Journal Article Sydowia, 76 , pp. 113-145, 2024, ISSN: 0082-0598. |
Virulence of Dothistroma septosporum and D. pini on Pinus nigra and P. mugo under conditions of natural infection Journal Article European Journal of Plant Pathology, 168 (2), pp. 775-785, 2024. |
Three new species in Russula subsection Xerampelinae supported by genealogical and phenotypic coherence Journal Article Mycologia, 116 (2), pp. 322-349, 2024. |
2023 |
Morfologická charakteristika peľových zŕn niektorých medonosných druhov rastlín Book AgroBioNet, Slovenská poľnohospodárska univerzita v Nitre, 2023, ISBN: 978–80–552‐2708‐5. |
Insecticidal activity of three plant extracts against adult Ips typograpgus L. under laboratory conditions Journal Article Baltic Journal of Coleopterology, 23 (2), pp. 139-158, 2023, ISSN: 1407 – 8619. |
Artificial inoculation and susceptibility of Pinus armandii to Dothistroma septosporum Journal Article Forest Pathology, 53 (5), pp. e12826, 2023, ISSN: 1439-0329. |
New regional records of Dothistroma needle blight pathogens from Slovakia: distribution, hosts and pathogens characterization Journal Article Annals of Forest Research, 66 (1), pp. 99-111, 2023, ISSN: 1844-8135. |
Climate, host and geography shape insect and fungal communities of trees Journal Article Scientific Reports, 13 , pp. 11570 , 2023, ISSN: 2045-2322. |
Hymenoscyphus fraxineus on Fraxinus excelsior in Slovakia: distribution and mating types Journal Article Biologia, 78 (5), pp. 1219-1230, 2023, ISSN: 0006-3088. |
Development and changes in pathogens population causing Dothistroma needle blight in Pinus nigra plantation in Strážovské vrchy Mts Journal Article Biologia, 78 (5), pp. 1231-1237, 2023, ISSN: 1336-9563. |
Checking the balance between pathogenic and mutualistic pine needle fungi of the genus Lophodermium in forested and urban areas of Slovakia Journal Article Scandinavian Journal of Forest Research, 38 (1-2), pp. 39-48, 2023, ISSN: 1651-1891. |
The increasing threat to European forests from the invasive foliar pine pathogen, Lecanosticta acicola Journal Article Forets Ecology and Management, 536 , pp. 120847, 2023. |
Population structure and diversity of the needle pathogen Dothistroma pini suggests human-mediated movement in Europe Journal Article Frontiers in Genetics, 14 , pp. 1103331, 2023, ISSN: 1338-7014. |
Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress Journal Article Folia Oecologica, 50 (1), pp. 1 - 15, 2023. |
2022 |
Diversity, migration routes, and worldwide population genetic structure of Lecanosticta acicola, the causal agent of brown spot needle blight Journal Article Molecular Plant Pathology, 23 (11), pp. 1620-1639, 2022, ISSN: 1364-3703. |
Low sensitivity of Pinus mugo to surface ozone pollution in the subalpine zone of continental Europe Journal Article International Journal of Biometeorology, 66 (11), pp. 2311–2324, 2022, ISSN: 0020-7128. |
Culturable endophytic fungi in Fraxinus excelsior and their interactions with Hymenoscyphus fraxineus Journal Article Forests, 13 (7), pp. 1-23, Article no. 1098, 2022, ISSN: 1999-4907. |
Entomopathogenic fungi associated with Stomoxys calcitrans in Slovakia and efficacy of local fungal strains against the stable fly Journal Article Acta fytotechnica et zootechnica, 25 (2), pp. 97-108, 2022, ISSN: ISSN 1336-9245. |
Comprehensive comparison of treatments for controlling the large pine weevil (Hylobius abietis) in Central Europe Journal Article Scientific Reports, 12 (9673), pp. 1-15, 2022, ISBN: 2045-2322. |
Phylogeny of Crepidotus applanatus Look-Alikes Reveals a Convergent Morphology Evolution and a New Species C. pini Journal Article Journal of Fungi, 8 (489), 2022, ISSN: 2309-608X. |
On the pathogenicity of Metarhizium pemphigi against Ips typographus L. Journal Article Proceedings of the Bulgarian Academy of Sciences, 75 (4), pp. 554-560, 2022, ISSN: 1310–1331. |
Insecticide activity of Greek oregano essential oil and entomopathogenic fungus Metarhizium pemphigi against Diabrotica virgifera virgifera LeConte Journal Article Forthcoming Cereal Research Communications, Forthcoming, ISSN: 1788-9170. |
Fungal Biodiversity Profiles 111-120 Journal Article Cryptogamie. Mycologie, 43 (2), pp. 23-61, 2022, ISSN: 1776-100. |
Worldwide diversity of endophytic fungi and insects associated with dormant tree twigs Journal Article Scientific Data, 9 (62 (2022)), pp. 1-9, 2022, ISSN: 2052-4463. |
Stability of trees infected by wood decay fungi estimated by acoustic tomography: a field survey Journal Article Trees Structure and Function, 36 (1), pp. 103-112, 2022, ISSN: 1432-2285. |
Plants, 11 , pp. 417, 2022, ISSN: 2223-7747. |
2021 |
Four new species of Russula subsection Roseinae from tropical montane forests in western Panama Journal Article PLoS ONE, 16 (10), pp. e0257616, 2021, ISSN: 1932-6203. |
Highly Clonal Structure and Abundance of One Haplotype Characterise the Diplodia sapinea Populations in Europe and Western Asia Journal Article Journal of Fungi, 7 (8), pp. 634, 2021, ISBN: EISSN 2309-608X. |
Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex Journal Article IMA Fungus, 20 (Article ID 12), pp. 1-31, 2021, ISSN: 2210-6359. |
Host range, genetic variability, and mating types of Lecanosticta acicola in Slovakia Journal Article Scandinavian Journal of Forest Research, 36 (5), pp. 325-332, 2021, ISSN: 0282-7581. |
Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia Journal Article Plant Pathology, 70 (8), pp. 1883-1896, 2021, ISSN: 1365-3059. |
Annals of Forest Research, 64 (1), pp. 129-145, 2021, ISSN: 2065-2445. |
Population structure and genetic diversity of Dothistroma septosporum in Slovakia Journal Article European Journal of Plant Pathology, 160 (4), pp. 771-787, 2021, ISSN: 1573-8469. |
Fusarium proliferatum causing dry rot of stored garlic in Slovakia Journal Article Journal of Plant Pathology, 2021, ISSN: 2239-7264. |
Morphological and genetic diversification of Russula floriformis, sp. nov., along the Isthmus of Panama Journal Article Mycologia, 113 (4), pp. 807-827, 2021, ISSN: 1557-2536. |
Divergence in culture morphology between two related species, Dothistroma septosporum and D. pini Journal Article Czech Mycology, 73 (1), pp. 109-119, 2021, ISSN: 1805-1421. |
Effect of Cadmium Chloride and Cadmium Nitrate on Growth and Mineral Nutrient Content in the Root of Fava Bean (Vicia faba L.) Journal Article Plants, 10 (5), pp. 1007, 2021. |
Erysiphe hypophylla, a second powdery mildew (Erysiphales) on oaks in Britain Journal Article Field Mycology, 22 (2), pp. 50-54, 2021, ISSN: 1468-1641. |
Infectious and parasitic diseases of phytophagous insect pests in the context of extreme environmental conditions Journal Article Central European Forestry Journal, 67 (2), pp. 72-84, 2021, ISSN: 2454-0358. |
Occurrence of gypsy moth (Lymantria dispar L.) in the Slovak Republic and its outbreaks during 1945–2020 Journal Article Central European Forestry Journal, 67 (2), pp. 55-71, 2021, ISSN: 2454-0358. |
Potential of Beauveria bassiana application via a carrier to control the large pine weevil Journal Article Crop Protection, 143 , pp. 105563, 2021, ISSN: 0261-2194. |
Journal of Fungi, 7 (2), pp. Article number: 111, 2021, ISBN: 2309-608X. |
The genus Dermoloma is more diverse than expected and forms a monophyletic lineage in the Tricholomataceae Journal Article Mycological Progress, 20 (1), pp. 11–25, 2021, ISSN: 1617-416X. |
Ash Trees (Fraxinus spp.) in Urban Greenery as Possible Invasion Gates of Non-Native Phyllactinia Species Journal Article Forests, 12 (2), pp. 183, 2021, ISSN: 1999-4907. |
2020 |
The response of Pinus species to ozone uptake in different climate regions of Europe Journal Article Central European Forestry Journal , 66 (4), pp. 255–268, 2020, ISBN: 2454-034X. |
Inhibition effect of selected inorganic metal ions on the mycelial growth of Cryphonectria parasitica Journal Article Journal of Plant Protection Research, 60 (4), pp. 399-405, 2020, ISSN: 1427-4345. |
Selected climatic variables in Slovakia are favourable to the development of Dothistroma needle blight Journal Article Folia Oecologica, 47 (2), pp. 144-152, 2020. |
Journal of Forest Science, 66 (10), pp. 420-435, 2020, ISSN: 1212-4834. |
Acta Zoologica Bulgarica, Supplementum 15 , pp. 89-96, 2020, ISSN: 0324-0770. |
Global Geographic Distribution and Host Range of Fusarium circinatum, the Causal Agent of Pine Pitch Canker Journal Article Forests, 11 (7), 2020, ISSN: 1999-4907. |
How variable is Crepidotus variabilis? Journal Article Phytotaxa, 449 (3), pp. 243-264, 2020, ISSN: 1179-3155. |
Host range of Hymenoscyphus fraxineus in Slovak arboreta Journal Article Forests, 11 (5), pp. 1-18, Article Number: 596, 2020, ISSN: 1999-4907. |
Spruce bark beetles as a biological waste that can be processed in a biogas plant Journal Article Turkish Journal of Agriculture and Forestry, 44 (2), pp. 169-179, 2020, ISSN: 1300-011X. |
One step closer to unravelling the origin of Russula: subgenus Glutinosae subg. nov. Journal Article Mycosphere, 11 (1), pp. 285–304, 2020, ISSN: 2077 7019. |
Hodophilus phaeophyllus complex (Clavariaceae, Agaricales) is defined as new phylogenetic lineage in Europe Journal Article Mycological Progress, 19 (2), pp. 111-125, 2020, ISSN: 1617-416X. |
Potential Interactions Between Invasive Fusarium circinatum and Other Pine Pathogens in Europe Journal Article Forests, 11 (1), 2020, ISSN: 1999-4907. |
2019 |
The quest for a globally comprehensible Russula language Journal Article Fungal Diversity, 99 (1), pp. 369-449, 2019, ISSN: 1560-2745. |
In vitro interactions between Trichoderma harzianum and pathogenic fungi damaging horse-chestnut (Aesculus hippocastanum) leaves and fruits Journal Article Biological Letters, 54 (1), pp. 21-35, 2019, ISSN: 1644-7700. |
Hypovirulence in chestnut blight fungus, Cryphonectria parasitica, in Slovakia Journal Article Biocontrol Science and Technology, 29 (9), pp. 840-851, 2019, ISSN: 0958-3157. |
Variance in leaf spot susceptibility in chestnut trees of different species and hybrids Journal Article Journal of Plant Disease and Protection, 126 (2), pp. 169-172, 2019, ISSN: 1861-3829. |
2018 |
Peľ a včelie peľové obnôžky z niektorých druhov rastlín Book Chapter Brindza, J; Motyleva, S (Ed.): Chapter 4, pp. 147, Slovenská poľnohospodárska univerzita v Nitre, Tr. A. Hlinku 2, 949 01 Nitra, FarmersEduca, 2018, ISBN: 978-80-552-1862-5. |
Grading of blight resistance in different chestnut accessions. Journal Article Acta Horticulturae, 1220 , pp. 87-94, 2018, ISSN: 05677572. |
Individual resistance of Fraxinus angustifolia and F. excelsior clones to Hymenoscyphus fraxineus Journal Article Journal of Plant Protection Research, 58 (3), pp. 227-233, 2018, ISSN: 1427-4345. |
Powdery mildew of California poppy caused by Erysiphe eschscholziae in Mexico Journal Article Canadian Journal of Plant Pathology, 40 (3), pp. 461-466, 2018, ISSN: 0706-0661 . |
Two blue-stain fungi colonizing Scots pine (Pinus sylvestris) trees infested by bark beetles in Slovakia, Central Europe Journal Article Biologia, 73 (11), pp. 1053-1066, 2018, ISSN: 0006-3088. |
From leaf to continent: The multi-scale distribution of an invasive cryptic pathogen complex on oak Journal Article Fungal Ecology, 36 , pp. 39-50, 2018, ISSN: 1754-5048. |
Mycobiota in dead and damaged branches of silver birch in Slovakia Journal Article Baltic Forestry, 24 (1), pp. 17-23, 2018, ISSN: 1392-1355. |
Screening for resistance of progenies derived from Castanea sativa × C. crenata and C. crenata to Cryphonectria parasitica Journal Article Forest Pathology, 48 (5), pp. e12439, 2018, ISSN: 1439-0329. |
Fungal Planet description sheets: 716-784 Journal Article Persoonia, 40 , pp. 240-393, 2018, ISSN: 1878-9080. |
European Hodophilus (Clavariaceae, Agaricales) species with yellow stipe Journal Article Mycological Progress, 17 (9), pp. 1097-1111, 2018, ISSN: ISSN: 1861-8952 . |
The potential of Beauveria bassiana inoculum formulated into a polymeric matrix for a microbial control of spruce bark beetle Journal Article Biocontrol Science and Technology, 28 (7), pp. 718-735, 2018, ISSN: 1360-0478. |
Needle blight caused by Dothistroma pini in Slovakia: distribution, host range and mating types Journal Article Scandinavian Journal of Forest Research, 33 (7), pp. 650-656, 2018, ISSN: 1651-1891. |
Species diversity of fungi on damaged branches and leaves of ashes (Fraxinus spp.) in different types of stands in Slovakia Journal Article Central European Forestry Journal, 64 (2), pp. 133-139, 2018, ISSN: 0323-1046. |
The hosts and geographic range of Dothistroma needle blight in Slovakia Journal Article Forest Pathology, 48 (3), pp. e12421, 2018, ISSN: 1437-4781. |
In planta bioassay on the effects of endophytic Beauveria strains against larvae of horse-chestnut leaf miner (Cameraria ohridella) Journal Article Biological Control, 121 , pp. 88-98, 2018, ISSN: 1049-9644. |
Biologia, 73 (1), pp. 53-65, 2018, ISSN: 1336-9563. |
Identification and characterization of the fungus Dothiorella sarmentorum on necrotic shoots of declining ash in Slovakia Journal Article Folia Oecologica, 45 (1), pp. 53-57, 2018, ISSN: 1336-5266. |
Sordaria fimicola-like ascomycete isolated from Pinus coulteri needles in Slovakia Journal Article Biologia, 73 (6), pp. 553-559, 2018, ISSN: ISSN 0006-3088. |
2017 |
Hypoderma rubi on two new hosts in Slovakia Journal Article Mycotaxon, 132 (4), pp. 849–855, 2017, ISSN: 0093-4666. |
Delimitation of European Crepidotus stenocystis as different from the North American species C. brunnescens (Crepidotaceae, Agariccales) Journal Article Phytotaxa, 328 (2), pp. 127-139, 2017, ISSN: 1179-3163. |
Hodophilus (Clavariaceae, Agaricales) species with dark dots on the stipe: more than one species in Europe Journal Article Mycological Progress, 16 (8), pp. 811–821, 2017, ISSN: 1861-8952 . |
Lecanicillium attenuatum isolates affecting the invasive cypress aphid (Cinara cupressi) in Chile Journal Article BioControl, 62 (5), pp. 625–637, 2017, ISSN: 1386-6141. |
Natural infection of Fraxinus angustifolia by Hymenoscyphus fraxineus in Slovakia Journal Article Baltic Forestry, 23 (1), pp. 52-55, 2017, ISSN: 2029-9230. |
Contribution to the pathogenic fungi on woody plants in North Korea Journal Article Phytotaxa, 299 (2), pp. 185-198, 2017, ISSN: 1179-3155. |
Extensively damaged trees tested with acoustic tomography considering tree stability in urban greenery Journal Article Trees Structure and Function, 31 (3), pp. 1015-1023, 2017, ISSN: 1432-2285. |
Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia Journal Article European Journal of Plant Pathology, 148 (2), pp. 283-294, 2017, ISSN: 0929-1873. |
Morphological features of Camarosporium pini – the fungus associated to health state degradation in Austrian and Ponderosa pine Journal Article Folia Oecologica, 44 (1), pp. 54-57, 2017, ISSN: 1336-5266. |
Clonostachys rosea associated with ponderosa and Coulter pine needles in Slovakia Journal Article Biologia, 72 (11), pp. 1258—1263, 2017, ISSN: ISSN 0006-3088. |
2016 |
Circumscription of species in the Hodophilus foetens complex (Clavariaceae, Agaricales) in Europe Journal Article Mycological Progress, 16 (1), pp. 47–62, 2016, ISSN: 1617-416X. |
Stephanitis takeyai and S. rhododendri (Heteroptera: Tingidae) in Slovakia: first record and economic importance Journal Article Plant Protection Research, 56 (2), pp. 193-198, 2016, ISBN: 1899-007X. |
Circumscription of species of Hodophilus (Clavariaceae, Agaricales) in North America with naphthalene odours Journal Article Botany, 94 (10), pp. 941-956, 2016, ISSN: 1916-2804. |
Plant Protection Science, 52 (3), pp. 199-208, 2016, ISSN: 1805-9341. |
The first record of tulip tree aphid, Illinoia liriodendri (Hemiptera: Aphididae), from Slovakia – Short Communication Journal Article Plant Protection Science, 52 (2), pp. 142-146, 2016, ISSN: 1212-2580. |
Genetic diversity and host range of powdery mildews on Papaveraceae Journal Article Mycological Progress, 15 (4, article 36), pp. 1-18, 2016, ISSN: 1617-416X. |
Biology and temperature requirements of the invasive seed bug Leptoglossus occidentalis (Heteroptera: Coreidae) in Europe. Journal Article Journal of Pest Science, 89 (1), pp. 31-44, 2016, ISSN: 1612-4758. |
The potential for Entomophaga maimaiga to regulate gypsy moth Lymantria dispar (L.) (Lepidoptera; Erebidae) in Europe Journal Article Journal of Applied Entomology, 140 (8), pp. 565–579, 2016, ISSN: 1439-0418. |
Comparison of two Coniochaeta species (C. ligniaria and C. malacotricha) with a new pathogen of black pine needles - Sordaria macrospora Journal Article Plant Protection Science, 52 (1), pp. 18-25, 2016. |
Comparison of fungal endophytes Pestalotia funerea and Truncatella hartigii isolated from some species of genus Pinus in morphological characteristics of conidia and appendages Journal Article Journal of Forest Science, 62 (6), pp. 279-284, 2016. |
First confirmed report on Fusarium sporotrichioides on Pinus ponderosa var. jeffreyi in Slovakia Journal Article Plant Protection Science, 52 (4), pp. 250-253, 2016. |
2015 |
Identification of Hymenoscyphus fraxineus, the causal agent of ash dieback in Slovakia Journal Article Biologia, 70 (5), pp. 559–564, 2015. |
Distribution and population structure of the chestnut blight fungus in Romania Journal Article Plant Protection Science, 51 (3), pp. 141-149, 2015. |
Škodcovia gaštana jedlého [Pests of European chestnut] Book Chapter Cagáň, Ľ (Ed.): Choroby a škodcovia záhradníckych rastlín, pp. 301-307, SPU v Nitre, Nitra, 1, 2015, ISBN: 978-80-552-1448-1. |
Škodcovia orecha kráľovského [Pest of walnut] Book Chapter Cagáň, Ľ (Ed.): Choroby a škodcovia záhradníckych rastlín, pp. 293-299, SPU v Nitre, Nitra, 1, 2015, ISBN: 978-80-552-1448-1. |
Škodcovia moruší [Pests of mulberries] Book Chapter Cagáň, Ľ (Ed.): Choroby a škodcovia záhradníckych rastlín, pp. 437-445, SPU v Nitre, Nitra, 1, 2015, ISBN: 978-80-552-1448-1. |
Význam starostlivosti o dreviny vo verejnej zeleni Proceeding Ústav ekológie lesa SAV Zvolen, Pobočka biológie drevín Nitra, Nitra, 2015, ISBN: 978-80-89408-19-1. |
Sordaria fimicola (Ascomycota, Sordariales) on Acer palmatum Journal Article Folia Oecologica, 42 (1), pp. 67-71, 2015. |
Fungi associated with a decline of Pinus nigra in urban greenery Journal Article Acta Fytotechnica et Zootechnica, 18 (2), pp. 36-43, 2015. |
Sordaria fimicola (Ascomycota, Sordariales) on Acer palmatum Journal Article Folia Oecologica, 42 (1), pp. 67-71, 2015, ISSN: 1336-5266. |
2014 |
Establishing a leaf proteome reference map for Ginkgo biloba provides insight into potential ethnobotanical uses Journal Article Journal of Agricultural and Food Chemistry, 62 (47), pp. 11547–11556, 2014. |
Neozygites species associated with aphids in Chile: current status and new reports Journal Article Mycotaxon, 129 (2), pp. 233-245, 2014, ISSN: 2154-8889. |
Global distribution of Erysiphe platani: new records, teleomorph formation and re-examination of herbarium collections Journal Article Cryptogamie, Mycologie, 35 (2), pp. 163-176, 2014, ISSN: 0181-1584. |
The lily magnolia powdery mildew Erysiphe magnifica found in Slovakia Journal Article Mycotaxon, 127 , pp. 51-57, 2014, ISSN: 0093-4666. |
First record of Entomophaga maimaiga (Entomophthorales: Entomophthoraceae) in Slovakia Journal Article Biocontrol Science and Technology, 24 (6), pp. 710-714, 2014, ISSN: 1360-0478. |
Morphogenetic soil classification system of Slovakia: basal reference taxonomy Book 2nd edition, Societas pedologica slovaca, Bratislava, 2014, ISBN: 978-80-8163-005-7. |
Príčiny hromadného hynutia gaštana jedlého [Causal agent of European chestnut blight] Journal Article Zahradnictví : časopis profesionálních zahradníků, 13 (7), pp. 48-51, 2014, ISSN: 1213-7596. |
Spread of chestnut blight in Slovakia in relation to the site topography and climatic characteristics Inproceedings Acta Horticulturae: Proceedings of the second European congress on chestnut : Debrecen, Hungary, Baia Mare, Romania, Modry Kamen, Slovakia, October 9-12, 2013. , pp. 35-42, ISHS, 2014, ISBN: 978 94 6261 032 3. |
Gaštan jedlý na Slovensku a v Európe : pestovanie, ochrana, variabilita a využitie [European chestnut in Slovakia : growing, protection, use and genetic variability] Book Garamond, Nitra, 2014, ISBN: 978-80-89408-18-4. |
Birch necrotic leaf spots caused by fungal pathogens Journal Article Folia Oecologica, 41 (2), 2014. |
Birch necrotic leaf spots caused by fungal pathogens Journal Article Folia Oecologica, 41 (2), pp. 134-140, 2014, ISSN: 1336-5266. |
Screening resistance to chestnut blight in young chestnut trees derived from Castanea sativa × C. crenata hybrids Journal Article Folia Oecologica, 41 (1), pp. 1-7, 2014, ISSN: 1336-5266. |
2013 |
A powdery mildew (Pseudoidium sp.) found on Chelidonium majus in the Czech Republic and Slovakia Journal Article Czech Mycology, 65 (1), pp. 125-133, 2013, ISSN: 1211-0981. |
Neozygites osornensis sp. nov., a fungal species causing mortality to the cypress aphid Cinara cupressi in Chile Journal Article Mycologia, 105 (3), pp. 661 – 669, 2013, ISSN: 1557-2536. |
Coniochaeta prunicola – first record for Slovakia and Europe Journal Article Central European Journal of Biology, 8 (2), pp. 195–200, 2013. |
Diversity of microfungi on branches of Castanea sativa in Slovakia [Diverzita mikroskopických húb na konároch Castanea sativa na Slovensku] Journal Article Polish Botanical Journal, 58 (2), pp. 741-746, 2013, ISSN: 1641-8180. |
Anatomic-morphological characteristic of fungus Coniochaeta prunicola isolated from Prunus cerasus leaves Journal Article Folia Oecologica, 40 (1), pp. 28-33, 2013. |
Coniochaeta prunicola-causal factor involved in health state decline of selected trees of the genus Prunus Journal Article Folia Oecologica, 40 (2), pp. 188-191, 2013. |
2012 |
New record of the fungus Coniochaeta prunicola on peaches from Slovakia Journal Article Biologia, 67 (2), pp. 269–273, 2012. |
Spread and population structure of Cryphonectria parasitica in a young chestnut orchard in Slovakia Journal Article Central European Journal of Biology, 7 (2), pp. 267–274, 2012. |
Gaštan jedlý na Slovensku. Perspektívy jeho ochrany a pestovania. Book Garmond, Nitra, 2012, ISBN: 978-80-89408-14-6 1. |
New record of the Coniochaeta prunicola on Prunus persica from Slovakia Journal Article Biologia (Bratislava), 67 (2), pp. 269-273, 2012. |
Shot-hole disease on Prunus persica - The morphology and biology of the Stigmina carpophila study Journal Article Folia Oecologica, 39 (1), pp. 21-27, 2012. |
2011 |
Horticultural science (Prague), 38 (2), pp. 54–62, 2011, ISSN: 1805-9333. |
Some of Hyphomycetes and Coelomycetes fungi isolated from affected leaves and twigs of cherry laurel trees Journal Article Folia Oecologica, 38 (2), pp. 137-145, 2011. |
2010 |
Effects of combining Beauveria bassiana and Nosema pyrausta on the mortality of Ostrinia nubilalis Journal Article Central European Journal of Biology, 5 (4), pp. 472–480, 2010, ISSN: 2391-5412. |
Effect of botanical insecticide Nimbecidine® on food consumption and egg hatchability of the terrestrial snail Monacha obstructa Journal Article Journal of Pest Science, 83 (1), pp. 27–32, 2010, ISSN: 1612-4758. |
Two important ascomycetes and their anamorphs on twigs of Betula pendula in Slovakia Journal Article Polish Botanical Journal, 55 (2), pp. 373-380, 2010, ISSN: 1641-8180. |
Influence of selected climatological characteristics on antracnose diseases development on plane trees Journal Article Ekológia (Bratislava), 29 (4), pp. 430-440, 2010. |
2009 |
Guignardia aesculi on species of Aesculus: new records from Europe and Asia Journal Article Mycotaxon, 108 , pp. 287-296, 2009, ISSN: 0093-4666. |
Horticultural Science , 36 (2), pp. 55-60, 2009. |
Needle disease on English yew caused by the parasitic fungus Cryptocline taxicola (All.) Petr. Journal Article Folia Oecologica, 36 (2), pp. 79-85, 2009. |
Species diversity of microscopic fungi on Austrian pines growing in urban greenery of Nitra town Journal Article Folia Oecologica, 37 (2), pp. 168-180, 2009. |
European record of Subramaniula thielavioides on opium poppy Journal Article Acta Mycologica, 44 (1), pp. 7-9, 2009, ISSN: 0001-625X. |
2008 |
European hornbeam powdery mildew Erysiphe arcuata in Slovakia Journal Article Journal of Phytopathology, 156 (10), pp. 597-601, 2008, ISSN: 0931-1785. |
Occurrence of lindenleaf spots and cultural characteristics of its agent–fungus Cercospora microsora Sacc. Journal Article Biológia, 63 (1), pp. 44-49, 2008. |
Cell wall regeneration of protoplasts isolated from Norvay spruce tissue cultures in a liquid nutrient medium Journal Article Folia Oecologica, 35 (2), pp. 18-24, 2008. |
Growth variability of Apiognomonia errabunda (Rob. & Desm.) Höhn. isolated from Tilia cordata Mill. Journal Article Acta Fytotechnica et Zootechnica, 11 (3), pp. 64-69, 2008. |
Neoerysiphe galeopsidis on Stachys species in Slovakia and the Czech Republic based on a re-examination of herbarium collections. Journal Article Czech Mycology, 60 (2), pp. 251-264, 2008, ISSN: 1211-0981. |
2007 |
Scopinella solani on graminicolous hosts in Slovakia and the Czech Republic Journal Article Mycotaxon, 102 , pp. 383-387, 2007, ISSN: 0093-4666. |
Influence of changed ecological conditions of environment on occurrence of London plane (Platanus x hispanica Münchh.) anthracnose Journal Article Folia Oecologica, 34 (1), pp. 1-8, 2007, ISBN: 1336-5266. |
2006 |
The anamorph of Erysiphe platani on Platanus ×hispanica in Slovakia Journal Article Mycotaxon, 97 , pp. 189-194, 2006, ISSN: 0093-4666. |
The Catalpa powdery mildew Erysiphe elevata in Slovakia Journal Article Cryptogamie, Mycologie, 27 (1), pp. 31-34, 2006, ISSN: 0181-1584. |
Leaf spots on lindens caused by fungi Cercospora microsora Sacc. and Apiognomonia tiliae (Rehm.) Höhn Journal Article Folia Oecologica, 33 (1), pp. 24-33, 2006. |
2004 |
Erysiphe catalpae and Erysiphe elevata in Europe Journal Article Mycological Progress, 3 (4), pp. 291-296, 2004, ISSN: 1617-416X. |
2002 |
Erysiphe flexuosa – a new species of powdery mildew for Slovakia Journal Article Biologia (Bratislava), 57 (4), pp. 437-440, 2002, ISSN: 0006-3088. |
Epidemic spread of Erysiphe flexuosa (North American powdery mildew disease of horse-chestnut) in Europe Journal Article Schlechtendalia, 8 , pp. 39-45, 2002, ISSN: 1436-2317. |