Mgr. Zuzana Jánošíková, PhD.

Slovak Academy of Sciences
Institute of Forest Ecology
Plant Pathology & Mycology Research Group
Akademická 2
949 01 Nitra
Phone:  +421 37 6943337
Email: janosikova@ife.sk

Research Interests: Tree pathology, diversity and distribution of fungal pathogens, pathogens ecology, biology and virulence, hosts susceptibility
Current research interest: Dothistroma needle blight, fungal pathogens of pines

Education:

• PhD., Faculty of Natural Sciences, Constantin The Philosopher University in Nitra, Slovak Republic, 2013 (nature protection and use of the landscape)
• MSc., Faculty of Natural Sciences, Constantin The Philosopher University in Nitra, Slovak Republic, 2009 (ecology)

Academic employment:

• Institute of Forest Ecology of Slovak Academy of Sciences
  • researcher (since 2013)

Projects:

• VEGA 2/0132/22 Impact of climate change on the distribution of selected pathogens of Pinus sp. trees  (2022-2025, project leader)
• MVTS Ochrana mestských stromov (2021-2025, project leader)
• VEGA 2/0077/18 Identification, genetic variability a pathogenicity of economically important needle cast species on pines (2018-2021, member of research team)
• VEGA 2/0062/18 Ash dieback: the casual agents and disease control strategy (2018-2021, member of research team)
• SK-FR-2017-0025 Spatial analyses in population genetic studies of tree pathogens (2018-2019, member of research team)
• VEGA 2/0069/14 Biology, spread and diagnostics of harmful agent Dothistroma septosporum (Mycosphaerella pini), D. pini and other harful agents of pines assimilation organs (2014-2017, member of research team)
• VEGA 2/0071/14 Species diversity and biological characteristics of parasitic fungi associated with damage and withering of woody plants (2014-2017, member of research team)

International projects:

• COST CA20132 Urban Tree Guard – Safeguarding European urban trees and forests through improved biosecurity (2021-2025, member of WG1 and WG2)
• COST FP 1403 Pine pitch canker – strategies for management of Gibberella circinata in greenhouses and forests (2015-2019)
• COST FP 1102 Determining invasiveness and risk of Dothistroma (2011-2015)

Interships:

• 2015 – short term scientific mission, STSM purpose: Genetic diversity of Dothistroma in Slovakia, Centre for Ecosystems, Society and Biosecurity, Forest Research, Farnham, Surrey, United Kingdom, Domain: Forests, their Products and Services COST Action Number FP1102 Determining Invasiveness And Risk Of Dothistroma (DIAROD)
• 2014 – short term scientific mission, STSM Purpose: Resistance Mechanisms in Scots Pine to infection by Dothistroma septosporum, University of Aberdeen, Aberdeen, Scotland, United Kingdom, Domain: Forests, their Products and Services COST Action Number FP1102 Determining Invasiveness And Risk Of Dothistroma (DIAROD)
• 2014 COST Action FP1102 DIAROD Training School on Molecular Detection and Population Genetics of Dothistroma Needle Blight Pathogens, Swedish University of Agricultural Sciences – SLU Uppsala, Sweden
• 2014 COST Action: FP1102 DIAROD Training School on: Field Detection and Surveillance of Dothistroma needle blight pathogens, Alice Holt Lodge, Farnham, United Kingdom
• 2010 – Training on approaches to sustainable development in mountain areas under global change – Erasmus Intensive Programme SUSTMONT, University of Innsbruck

List of my publications:

2024
	Jánošíková, Z; Kobza, M; Ondrušková, E; Ostrovský, R; Pažitný, J; Adamčíková, K Virulence of Dothistroma septosporum and D. pini on Pinus nigra and P. mugo under conditions of natural infection Journal Article European Journal of Plant Pathology, 168 (2), pp. 775-785, 2024. Abstract | Links | BibTeX @article{Jánošíková2024, title = {Virulence of Dothistroma septosporum and D. pini on Pinus nigra and P. mugo under conditions of natural infection}, author = {Z. Jánošíková and M. Kobza and E. Ondrušková and R. Ostrovský and J. Pažitný and K. Adamčíková}, url = {http://ife.sk/wp-content/uploads/2020/09/Fig_edited.jpghttp://ife.sk/wp-content/uploads/2020/09/final-1-2.jpg}, doi = {https://doi.org/10.1007/s10658-023-02799-5}, year = {2024}, date = {2024-04-04}, journal = {European Journal of Plant Pathology}, volume = {168}, number = {2}, pages = {775-785}, abstract = {Dothistroma needle blight (DNB) is a severe needle disease of pines worldwide, caused by two closely related species, Dothistroma septosporum and D. pini. The two fungal species are similar not only in their morphological characteristics, but also cause very similar symptoms in their hosts, and have a similar ecology. The aim of this study was to compare the virulence of the two Dothistroma species in natural infection experiments on 2-year-old seedlings of two DNB susceptible pine species, Pinus nigra and P. mugo, in two seedling stands for each pathogen species. The virulence of the pathogens and presence of symptoms (symptomatic needles, red bands and acervuli) were assessed after 2 years of exposure to inoculum. The incidence of seedlings with DNB symptoms was 65% and 76% for P. nigra and P. mugo, respectively. No difference was found between D. septosporum and D. pini in any of the three DNB symptoms evaluated on seedlings of P. mugo. However, symptoms of disease differed between the two Dothistroma species on P. nigra. Variables, which reflect the intensity of disease development, the number of red bands and acervuli per needle, showed a difference in virulence between D. septosporum and D. pini, but only in the case of the host species P. nigra. The results suggest that the virulence of the two Dothistroma species could be affected by host pine species and that there are differences in susceptibility of individual pine species to D. septosporum and D. pini. Further factors could affect the virulence of these pathogens, including isolate origin, climatic or environmental factors.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Dothistroma needle blight (DNB) is a severe needle disease of pines worldwide, caused by two closely related species, Dothistroma septosporum and D. pini. The two fungal species are similar not only in their morphological characteristics, but also cause very similar symptoms in their hosts, and have a similar ecology. The aim of this study was to compare the virulence of the two Dothistroma species in natural infection experiments on 2-year-old seedlings of two DNB susceptible pine species, Pinus nigra and P. mugo, in two seedling stands for each pathogen species. The virulence of the pathogens and presence of symptoms (symptomatic needles, red bands and acervuli) were assessed after 2 years of exposure to inoculum. The incidence of seedlings with DNB symptoms was 65% and 76% for P. nigra and P. mugo, respectively. No difference was found between D. septosporum and D. pini in any of the three DNB symptoms evaluated on seedlings of P. mugo. However, symptoms of disease differed between the two Dothistroma species on P. nigra. Variables, which reflect the intensity of disease development, the number of red bands and acervuli per needle, showed a difference in virulence between D. septosporum and D. pini, but only in the case of the host species P. nigra. The results suggest that the virulence of the two Dothistroma species could be affected by host pine species and that there are differences in susceptibility of individual pine species to D. septosporum and D. pini. Further factors could affect the virulence of these pathogens, including isolate origin, climatic or environmental factors. Close http://ife.sk/wp-content/uploads/2020/09/Fig_edited.jpghttp://ife.sk/wp-content/[...] doi:https://doi.org/10.1007/s10658-023-02799-5 Close
2023
	Jánošíková, Z; Ondrušková, E; Adamčíková, K Artificial inoculation and susceptibility of Pinus armandii to Dothistroma septosporum Journal Article Forest Pathology, 53 (5), pp. e12826, 2023, ISSN: 1439-0329. Abstract | Links | BibTeX @article{Jánošíková2023b, title = {Artificial inoculation and susceptibility of Pinus armandii to Dothistroma septosporum}, author = {Z. Jánošíková and E. Ondrušková and K. Adamčíková}, doi = {DOI: 10.1111/efp.12826 }, issn = {1439-0329}, year = {2023}, date = {2023-08-18}, journal = {Forest Pathology}, volume = {53}, number = {5}, pages = {e12826}, abstract = {Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species. Close doi:DOI: 10.1111/efp.12826 Close
	Adamčíková, K; Pastirčáková, K; Jánošíková, Z; Ostrovský, R; Pastirčák, M; Pažitný, J; Kobza, M; Adamčík, S; Kádasi-Horáková, M; Ondrušková, E New regional records of Dothistroma needle blight pathogens from Slovakia: distribution, hosts and pathogens characterization Journal Article Annals of Forest Research, 66 (1), pp. 99-111, 2023, ISSN: 1844-8135. Abstract | Links | BibTeX @article{Adamčíková2023, title = {New regional records of Dothistroma needle blight pathogens from Slovakia: distribution, hosts and pathogens characterization}, author = {K. Adamčíková and K. Pastirčáková and Z. Jánošíková and R. Ostrovský and M. Pastirčák and J. Pažitný and M. Kobza and S. Adamčík and M. Kádasi-Horáková and E. Ondrušková}, doi = {https://doi.org/10.15287/afr.2023.2427}, issn = {1844-8135}, year = {2023}, date = {2023-07-25}, journal = {Annals of Forest Research}, volume = {66}, number = {1}, pages = {99-111}, abstract = {Dothistroma needle blight is one of the most severe needle diseases of pines caused by two closely related species, Dothistroma septosporum and D. pini. The further spread and distribution of this disease was investigated in Slovakia as well as new hosts and stand types were identified at regional level. Dothistroma septosporum was recorded in a natural stand at higher altitude on Pinus cembra in the High Tatras and the P. uncinata records are new host reports for Slovakia for this pathogen. Moreover, for D. pini, P. cembra as a new host at the country level was recorded and P. armandii was identified as new host species worldwide. Mating types for all collected samples and ITS haplotypes for D. pini isolates were determined. For D. pini, five localities with the presence of both mating types and three ITS haplotypes (Dp_HAP.1, Dp_HAP.2 and Dp_HAP.4) were reported. Samples where both mating types of the pathogens were identified, were selected for the microscopic examination of fruiting bodies aimed to detect sexual reproductive organs. In all inspected needle samples of D. pini, only conidiomata with typical hyaline cylindrical conidia were identified. The sexual state of D. septosporum was recorded in one sample of P. nigra needles.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Dothistroma needle blight is one of the most severe needle diseases of pines caused by two closely related species, Dothistroma septosporum and D. pini. The further spread and distribution of this disease was investigated in Slovakia as well as new hosts and stand types were identified at regional level. Dothistroma septosporum was recorded in a natural stand at higher altitude on Pinus cembra in the High Tatras and the P. uncinata records are new host reports for Slovakia for this pathogen. Moreover, for D. pini, P. cembra as a new host at the country level was recorded and P. armandii was identified as new host species worldwide. Mating types for all collected samples and ITS haplotypes for D. pini isolates were determined. For D. pini, five localities with the presence of both mating types and three ITS haplotypes (Dp_HAP.1, Dp_HAP.2 and Dp_HAP.4) were reported. Samples where both mating types of the pathogens were identified, were selected for the microscopic examination of fruiting bodies aimed to detect sexual reproductive organs. In all inspected needle samples of D. pini, only conidiomata with typical hyaline cylindrical conidia were identified. The sexual state of D. septosporum was recorded in one sample of P. nigra needles. Close doi:https://doi.org/10.15287/afr.2023.2427 Close
	Ondrušková, E; Adamčík, S; Kobza, M; Jánošíková, Z; Ostrovský, R; Pastirčáková, K; Caboň, M; Adamčíková, K Checking the balance between pathogenic and mutualistic pine needle fungi of the genus Lophodermium in forested and urban areas of Slovakia Journal Article Scandinavian Journal of Forest Research, 38 (1-2), pp. 39-48, 2023, ISSN: 1651-1891. Abstract | Links | BibTeX @article{Ondrušková2023, title = {Checking the balance between pathogenic and mutualistic pine needle fungi of the genus Lophodermium in forested and urban areas of Slovakia}, author = {E. Ondrušková and S. Adamčík and M. Kobza and Z. Jánošíková and R. Ostrovský and K. Pastirčáková and M. Caboň and K. Adamčíková }, doi = { DOI: 10.1080/02827581.2023.2191004}, issn = {1651-1891}, year = {2023}, date = {2023-03-27}, journal = {Scandinavian Journal of Forest Research}, volume = {38}, number = {1-2}, pages = {39-48}, abstract = {Lophodermium species are well known to be among dominant endophytic fungi colonising pine needles. Occurrence of two species with different life strategies on different Pinus trees across Slovakia was detected by PCR using species specific primers. In general, commensalistic species L. pinastri was more frequent than parasitic L. seditiosum. There were no significant differences observed between urban and natural environments, but natural environments have more frequent colonisation by L. pinastri and less frequent were trees negatively tested to Lophodermium colonisation. Among the most frequently sampled trees were non-native P. nigra and native P. sylvestris. Significant difference in Lophodermium incidence was detected between these two species in natural environment, with increased frequency of L. pinastri on native Scots pine. In addition to one clade of L. pinastri, culture based species identification confirmed presence of L. corconticum and an undescribed clade of L. seditiosum that is not sensitive to used PCR primers for the species detection.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Lophodermium species are well known to be among dominant endophytic fungi colonising pine needles. Occurrence of two species with different life strategies on different Pinus trees across Slovakia was detected by PCR using species specific primers. In general, commensalistic species L. pinastri was more frequent than parasitic L. seditiosum. There were no significant differences observed between urban and natural environments, but natural environments have more frequent colonisation by L. pinastri and less frequent were trees negatively tested to Lophodermium colonisation. Among the most frequently sampled trees were non-native P. nigra and native P. sylvestris. Significant difference in Lophodermium incidence was detected between these two species in natural environment, with increased frequency of L. pinastri on native Scots pine. In addition to one clade of L. pinastri, culture based species identification confirmed presence of L. corconticum and an undescribed clade of L. seditiosum that is not sensitive to used PCR primers for the species detection. Close doi: DOI: 10.1080/02827581.2023.2191004 Close
2021
	Adamčíková, K; Jánošíková, Z; Adamčík, S; Ostrovský, R; Pastirčáková, K; Kobza, M; Ondrušková, E Host range, genetic variability, and mating types of Lecanosticta acicola in Slovakia Journal Article Scandinavian Journal of Forest Research, 36 (5), pp. 325-332, 2021, ISSN: 0282-7581. Abstract | Links | BibTeX @article{Adamčíková2021b, title = {Host range, genetic variability, and mating types of \textit{Lecanosticta acicola} in Slovakia}, author = {K. Adamčíková and Z. Jánošíková and S. Adamčík and R. Ostrovský and K. Pastirčáková and M. Kobza and E. Ondrušková}, url = {https://doi.org/10.1080/02827581.2021.1941236}, doi = {10.1080/02827581.2021.1941236}, issn = {0282-7581}, year = {2021}, date = {2021-07-16}, journal = {Scandinavian Journal of Forest Research}, volume = {36}, number = {5}, pages = {325-332}, abstract = {In recent years, there has been an apparent Europe-wide emerging presence of brown spot needle blight, a disease of pine species caused by the fungus Lecanosticta acicola. In this study, we report the first well-documented occurrences of the pathogen in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region and TEF1 gene. Among the material collected from 84 locations within the country, L. acicola was present in 17 samples from 13 different locations, mainly distributed in urban environments. Four pine species were identified as hosts, among which, Pinus nigra and P. mugo were found to be the most frequently infected. Analysis of the mating type genes of 24 isolates obtained from two localities revealed the presence of a single mating type in Slovakia. All analyzed ITS sequences of the Slovak isolates were found to be uniform. However, although analysis of the TEF1 gene indicated that all Slovak isolates could be grouped into a single lineage, we detected nucleotide polymorphisms suggestive of a certain degree of genetic diversification within central European populations of the fungus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close In recent years, there has been an apparent Europe-wide emerging presence of brown spot needle blight, a disease of pine species caused by the fungus Lecanosticta acicola. In this study, we report the first well-documented occurrences of the pathogen in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region and TEF1 gene. Among the material collected from 84 locations within the country, L. acicola was present in 17 samples from 13 different locations, mainly distributed in urban environments. Four pine species were identified as hosts, among which, Pinus nigra and P. mugo were found to be the most frequently infected. Analysis of the mating type genes of 24 isolates obtained from two localities revealed the presence of a single mating type in Slovakia. All analyzed ITS sequences of the Slovak isolates were found to be uniform. However, although analysis of the TEF1 gene indicated that all Slovak isolates could be grouped into a single lineage, we detected nucleotide polymorphisms suggestive of a certain degree of genetic diversification within central European populations of the fungus. Close https://doi.org/10.1080/02827581.2021.1941236 doi:10.1080/02827581.2021.1941236 Close
	Adamčíková, K; Jánošíková, Z; van der Nest, A; Adamčík, S; Ondrušková, E; Barnes, I Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia Journal Article Plant Pathology, 70 (8), pp. 1883-1896, 2021, ISSN: 1365-3059. Abstract | Links | BibTeX @article{Adamčíková2021c, title = {Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia}, author = {K. Adamčíková and Z. Jánošíková and A. van der Nest and S. Adamčík and E. Ondrušková and I. Barnes }, url = {https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13428}, doi = {10.1111/ppa.13428}, issn = {1365-3059}, year = {2021}, date = {2021-06-30}, journal = {Plant Pathology}, volume = {70}, number = {8}, pages = {1883-1896}, abstract = {Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia. Close https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13428 doi:10.1111/ppa.13428 Close
	Jánošíková, Z; Dutech, C; Ondrušková, E; Adamčíková, K; Mullett, M Population structure and genetic diversity of Dothistroma septosporum in Slovakia Journal Article European Journal of Plant Pathology, 160 (4), pp. 771-787, 2021, ISSN: 1573-8469. Abstract | Links | BibTeX @article{Jánošíková2021, title = {Population structure and genetic diversity of Dothistroma septosporum in Slovakia}, author = {Z. Jánošíková and C. Dutech and E. Ondrušková and K. Adamčíková and M. Mullett}, url = {https://link.springer.com/article/10.1007/s10658-021-02266-z}, doi = {doi:10.1007/s10658-021-02266-z}, issn = {1573-8469}, year = {2021}, date = {2021-06-23}, journal = {European Journal of Plant Pathology}, volume = {160}, number = {4}, pages = {771-787}, abstract = {Dothistroma needle blight (DNB) is a serious pine disease present worldwide caused by the ascomycetous fungi Dothistroma septosporum and Dothistroma pini. Based on multiplex PCR analysis of 11 microsatellite markers screened on 253 D. septosporum isolates obtained from 32 sites across Slovakia, a total of 137 unique multilocus haplotypes (MLHs) were detected. The majority of MLHs (n = 91) were represented by a single isolate, but 13 MLHs were found at two different sites, and in some cases, these were separated by large distances. Four population clusters were identified using a discriminant analysis of principal components (DAPC). The genetic clusters obtained from the DAPC were mixed throughout the country and were not restricted to particular host species. Although both mating types of D. septosporum were found to be in an almost perfect 1:1 ratio in both the non-clone-corrected and clone-corrected datasets, random mating was rejected in the entire dataset. Random mating was only supported in the Pinus mugo group planted in urban areas and on a smaller spatial scale in two sites (Kálnica and Litava) using the clonecorrected dataset. These findings suggest a mixed mode of reproduction with an important component of sexual reproduction, although the sexual stage of the fungus (teleomorph) has not been physically observed in Slovakia yet. The examination of spatial relationships using spatial principal component analyses and the presence of isolation-by-distance together with relatively high genetic diversity suggests the pathogen has been long established in Slovakia and spread naturally across the landscape. However, the weak population structure and findings of identical clones at widely separated sites strongly suggests some degree of human assisted dispersal.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Dothistroma needle blight (DNB) is a serious pine disease present worldwide caused by the ascomycetous fungi Dothistroma septosporum and Dothistroma pini. Based on multiplex PCR analysis of 11 microsatellite markers screened on 253 D. septosporum isolates obtained from 32 sites across Slovakia, a total of 137 unique multilocus haplotypes (MLHs) were detected. The majority of MLHs (n = 91) were represented by a single isolate, but 13 MLHs were found at two different sites, and in some cases, these were separated by large distances. Four population clusters were identified using a discriminant analysis of principal components (DAPC). The genetic clusters obtained from the DAPC were mixed throughout the country and were not restricted to particular host species. Although both mating types of D. septosporum were found to be in an almost perfect 1:1 ratio in both the non-clone-corrected and clone-corrected datasets, random mating was rejected in the entire dataset. Random mating was only supported in the Pinus mugo group planted in urban areas and on a smaller spatial scale in two sites (Kálnica and Litava) using the clonecorrected dataset. These findings suggest a mixed mode of reproduction with an important component of sexual reproduction, although the sexual stage of the fungus (teleomorph) has not been physically observed in Slovakia yet. The examination of spatial relationships using spatial principal component analyses and the presence of isolation-by-distance together with relatively high genetic diversity suggests the pathogen has been long established in Slovakia and spread naturally across the landscape. However, the weak population structure and findings of identical clones at widely separated sites strongly suggests some degree of human assisted dispersal. Close https://link.springer.com/article/10.1007/s10658-021-02266-z doi:doi:10.1007/s10658-021-02266-z Close
	Adamčíková, K; Ondrušková, E; Pažitný, J; Jánošíková, Z Divergence in culture morphology between two related species, Dothistroma septosporum and D. pini Journal Article Czech Mycology, 73 (1), pp. 109-119, 2021, ISSN: 1805-1421. Abstract | Links | BibTeX @article{Adamčíková2021, title = {Divergence in culture morphology between two related species, Dothistroma septosporum and D. pini}, author = {K. Adamčíková and E. Ondrušková and J. Pažitný and Z. Jánošíková}, url = {http://ife.sk/wp-content/uploads/2020/09/fig.-2-rev-5.tif}, doi = {doi.org/10.33585/cmy.73108}, issn = {1805-1421}, year = {2021}, date = {2021-05-24}, journal = {Czech Mycology}, volume = {73}, number = {1}, pages = {109-119}, abstract = {Dothistroma needle blight (DNB) is one of the most important pine needle diseases worldwide. The disease is caused by two fungal species, Dothistroma septosporum and D. pini, which are similar not only in terms of their ecology, causing similar symptoms in hosts of the genus Pinus, but also in terms of their morphological characteristics. The morphologies of 353 cultured Dothistroma isolates from 11 different pine species in Slovakia and their relation to Dothistroma species and/or fungus origin (host) were studied and evaluated by means of the Chi-square test. The cultures were classified into eight categories according to pigmentation. Dothistroma pini isolates were assigned to 4 of the 8 categories, and D. septosporum isolates were assigned to all 8 categories with varying frequencies. The Chi-square test revealed that the culture morphology categories of D. pini and D. septosporum differed significantly. Interactions between the evaluated factors (culture morphology, Dothistroma and host species) were analysed and showed significant differences.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Dothistroma needle blight (DNB) is one of the most important pine needle diseases worldwide. The disease is caused by two fungal species, Dothistroma septosporum and D. pini, which are similar not only in terms of their ecology, causing similar symptoms in hosts of the genus Pinus, but also in terms of their morphological characteristics. The morphologies of 353 cultured Dothistroma isolates from 11 different pine species in Slovakia and their relation to Dothistroma species and/or fungus origin (host) were studied and evaluated by means of the Chi-square test. The cultures were classified into eight categories according to pigmentation. Dothistroma pini isolates were assigned to 4 of the 8 categories, and D. septosporum isolates were assigned to all 8 categories with varying frequencies. The Chi-square test revealed that the culture morphology categories of D. pini and D. septosporum differed significantly. Interactions between the evaluated factors (culture morphology, Dothistroma and host species) were analysed and showed significant differences. Close http://ife.sk/wp-content/uploads/2020/09/fig.-2-rev-5.tif doi:doi.org/10.33585/cmy.73108 Close
	Mullett, M S; Drenkhan, R; Adamson, K; Boroń, P; Lenart-Boroń, A; Barnes, I; Tomšovský, M; Jánošíková, Z; Adamčíková, K; Ondrušková, E; Queloz, V; Piškur, B; Musolin, D L; Davydenko, K; Georgieva, M; Schmitz, S; Kačergius, A; Ghelardini, L; Orlović, Kranjec J; Müller, M; Oskay, F; Hauptman, T; Halász, Á; Markovskaja, S; Solheim, H; Vuorinen, M; Heinzelmann, R; Hamelin, R C; Konečný, A Worldwide Genetic Structure Elucidates the Eurasian Origin and Invasion Pathways of Dothistroma septosporum, Causal Agent of Dothistroma Needle Blight Journal Article Journal of Fungi, 7 (2), pp. Article number: 111, 2021, ISBN: 2309-608X. Abstract | Links | BibTeX @article{Mullett2021, title = {Worldwide Genetic Structure Elucidates the Eurasian Origin and Invasion Pathways of Dothistroma septosporum, Causal Agent of Dothistroma Needle Blight }, author = {M. S. Mullett and R. Drenkhan and K. Adamson and P. Boroń and A. Lenart-Boroń and I. Barnes and M. Tomšovský and Z. Jánošíková and K. Adamčíková and E. Ondrušková and V. Queloz and B. Piškur and D.L. Musolin and K. Davydenko and M. Georgieva and S. Schmitz and A. Kačergius and L. Ghelardini and J. Kranjec Orlović and M. Müller and F. Oskay and T. Hauptman and Á. Halász and S. Markovskaja and H. Solheim and M. Vuorinen and R. Heinzelmann and R.C. Hamelin and A. Konečný}, doi = {doi 10.3390/jof7020111 }, isbn = {2309-608X}, year = {2021}, date = {2021-02-03}, journal = {Journal of Fungi}, volume = {7}, number = {2}, pages = {Article number: 111}, abstract = {Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia. Close doi:doi 10.3390/jof7020111 Close
2020
	Adamčíková, K; Jánošíková, Z; Pažitný, J Inhibition effect of selected inorganic metal ions on the mycelial growth of Cryphonectria parasitica Journal Article Journal of Plant Protection Research, 60 (4), pp. 399-405, 2020, ISSN: 1427-4345. Abstract | Links | BibTeX @article{Adamčíková2020, title = {Inhibition effect of selected inorganic metal ions on the mycelial growth of Cryphonectria parasitica}, author = {K. Adamčíková and Z. Jánošíková and J. Pažitný}, doi = {DOI: 10.24425/jppr.2020.134915}, issn = {1427-4345}, year = {2020}, date = {2020-12-02}, journal = {Journal of Plant Protection Research}, volume = {60}, number = {4}, pages = {399-405}, abstract = {In the current study the antifungal activity of inorganic reagents was tested against Cryphonectria parasitica in vitro in a mycelial growth inhibition test. Three reagents, each consisting of chloride silver (AgCl) in combination with (1) aluminum oxide, (2) zinc oxide − ZnO, and (3) aluminum oxide and titanium dioxide were tested. Significant differences of the tested reagents on the growth of C. parasitica were recorded. The study demonstrated that silver in mixture with ZnO had an antifungal effect and significantly reduced the mycelial growth of C. parasitica in vitro. The mixture of AgCl with the other two combinations of inorganic metal oxides had no inhibition effect on the growth of the pathogen. It was confirmed that ZnO (applied in a single compound test) is responsible for inhibition of C. parasitica mycelium growth. A preliminary in planta assay was performed but statistically significant differences were not recorded in the average increment of canker length.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close In the current study the antifungal activity of inorganic reagents was tested against Cryphonectria parasitica in vitro in a mycelial growth inhibition test. Three reagents, each consisting of chloride silver (AgCl) in combination with (1) aluminum oxide, (2) zinc oxide − ZnO, and (3) aluminum oxide and titanium dioxide were tested. Significant differences of the tested reagents on the growth of C. parasitica were recorded. The study demonstrated that silver in mixture with ZnO had an antifungal effect and significantly reduced the mycelial growth of C. parasitica in vitro. The mixture of AgCl with the other two combinations of inorganic metal oxides had no inhibition effect on the growth of the pathogen. It was confirmed that ZnO (applied in a single compound test) is responsible for inhibition of C. parasitica mycelium growth. A preliminary in planta assay was performed but statistically significant differences were not recorded in the average increment of canker length. Close doi:DOI: 10.24425/jppr.2020.134915 Close
	Ondrušková, Emília; Ostrovský, Radovan; Jánošíková, Zuzana; Adamčíková, Katarína; Kobza, Marek Selected climatic variables in Slovakia are favourable to the development of Dothistroma needle blight Journal Article Folia Oecologica, 47 (2), pp. 144-152, 2020. Abstract | Links | BibTeX @article{Ondrušková2020b, title = {Selected climatic variables in Slovakia are favourable to the development of Dothistroma needle blight}, author = {Emília Ondrušková and Radovan Ostrovský and Zuzana Jánošíková and Katarína Adamčíková and Marek Kobza}, url = {http://ife.sk/wp-content/uploads/2020/09/foecol-2020-0008.pdf}, doi = {10.2478/foecol-2020-0017}, year = {2020}, date = {2020-11-24}, journal = {Folia Oecologica}, volume = {47}, number = {2}, pages = {144-152}, abstract = {Dothistroma needle blight (DNB) is a disease of pine needles. It causes significant defoliation of affected trees; intensive attacks lead to damages of the whole stand. The relationship of trends in disease severity and intensity with climatic variables were studied in three Austrian pine plantations (Jahodná, Kálnica, Litava) during 2014–2018. During the monitoring period, the greatest variability in disease severity was observed in the top third of the crowns, which showed the highest correlation with the variants of the most important climatic conditions (temperature and relative humidity) tested. For the spread of DNB, based on statistical assessment, a higher number of intervals of environmentally favourable climatic conditions is crucial. Both Dothistroma species (D. septosporum and D. pini), which are causal agents of the disease, were identified in Jahodná. In Kálnica and Litava, only D. septosporum was present.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Dothistroma needle blight (DNB) is a disease of pine needles. It causes significant defoliation of affected trees; intensive attacks lead to damages of the whole stand. The relationship of trends in disease severity and intensity with climatic variables were studied in three Austrian pine plantations (Jahodná, Kálnica, Litava) during 2014–2018. During the monitoring period, the greatest variability in disease severity was observed in the top third of the crowns, which showed the highest correlation with the variants of the most important climatic conditions (temperature and relative humidity) tested. For the spread of DNB, based on statistical assessment, a higher number of intervals of environmentally favourable climatic conditions is crucial. Both Dothistroma species (D. septosporum and D. pini), which are causal agents of the disease, were identified in Jahodná. In Kálnica and Litava, only D. septosporum was present. Close http://ife.sk/wp-content/uploads/2020/09/foecol-2020-0008.pdf doi:10.2478/foecol-2020-0017 Close
2018
	Ondrušková, E; Jánošíková, Z; Adamčík, S; Kádasi-Horáková, M; Rákusová-Sládková, D; Adamčíková, K Needle blight caused by Dothistroma pini in Slovakia: distribution, host range and mating types Journal Article Scandinavian Journal of Forest Research, 33 (7), pp. 650-656, 2018, ISSN: 1651-1891. Abstract | Links | BibTeX @article{Ondrušková2018, title = {Needle blight caused by Dothistroma pini in Slovakia: distribution, host range and mating types}, author = {E. Ondrušková and Z. Jánošíková and S. Adamčík and M. Kádasi-Horáková and D. Rákusová-Sládková and K. Adamčíková}, doi = {10.1080/02827581.2018.1482954}, issn = {1651-1891}, year = {2018}, date = {2018-06-06}, journal = {Scandinavian Journal of Forest Research}, volume = {33}, number = {7}, pages = {650-656}, abstract = {Dothistroma needle blight (DNB) has been observed in Slovakia during the last two decades. Up until 2017, Dothistroma septosporum has only been detected and molecularly confirmed to cause DNB in Slovakia. Here, we report the detection of Dothistroma pini at six localities around Slovakia, representing different plantation types. Four pine species (Pinus sylvestris, P. nigra, P. mugo and P. jeffreyi) were confirmed as hosts of D. pini in Slovakia, of which only P. mugo has been previously reported as host in Slovakia. Three gene regions (ITS, EF1 –α, and ß-tubulin) of each of the 13 isolates were sequenced and assigned as D. pini. Based on ITS sequences, the studied isolates represent the haplotypes Dp_HAP.1, Dp_HAP.2. Both mating types were detected but at different localities. Our results suggest that in addition to D. septosporum, D. pini may contribute to DNB also in Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close Dothistroma needle blight (DNB) has been observed in Slovakia during the last two decades. Up until 2017, Dothistroma septosporum has only been detected and molecularly confirmed to cause DNB in Slovakia. Here, we report the detection of Dothistroma pini at six localities around Slovakia, representing different plantation types. Four pine species (Pinus sylvestris, P. nigra, P. mugo and P. jeffreyi) were confirmed as hosts of D. pini in Slovakia, of which only P. mugo has been previously reported as host in Slovakia. Three gene regions (ITS, EF1 –α, and ß-tubulin) of each of the 13 isolates were sequenced and assigned as D. pini. Based on ITS sequences, the studied isolates represent the haplotypes Dp_HAP.1, Dp_HAP.2. Both mating types were detected but at different localities. Our results suggest that in addition to D. septosporum, D. pini may contribute to DNB also in Slovakia. Close doi:10.1080/02827581.2018.1482954 Close
	Jánošíková-Hečková, Z; Ondrušková, E; Barta, M; Ostrovský, R; Kádasi-Horáková, M; Pastirčáková, K; Kobza, M; Adamčíková, K The hosts and geographic range of Dothistroma needle blight in Slovakia Journal Article Forest Pathology, 48 (3), pp. e12421, 2018, ISSN: 1437-4781. Abstract | Links | BibTeX @article{Jánošíková-Hečková2018, title = {The hosts and geographic range of Dothistroma needle blight in Slovakia}, author = {Z. Jánošíková-Hečková and E. Ondrušková and M. Barta and R. Ostrovský and M. Kádasi-Horáková and K. Pastirčáková and M. Kobza and K. Adamčíková }, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/efp.12421}, doi = {10.1111/efp.12421}, issn = {1437-4781}, year = {2018}, date = {2018-02-15}, journal = {Forest Pathology}, volume = {48}, number = {3}, pages = {e12421}, abstract = {The occurrence and distribution of Dothistroma needle blight (DNB) were studied in 2014–2017 around Slovakia. A total of 84 localities, both native and planted, were investigated, and the presence of DNB was confirmed in 73 of them. In all positive locations, symptoms typical of DNB were observed and the Dothistroma species was confirmed using species-specific primers either from fungal cultures or directly from needles. Both Dothistroma species—D. septosporum and D. pini—were identified. Both species occurred together in 29 locations, only D. septosporum in 42 and only D. pini in two locations. The host range of D. septosporum included 10 pine species and two spruce species. The host range of D. pini comprised the same number of pine hosts but only one spruce species. Five pine hosts, P. aristata, P. coulteri, P. densiflora, P. jeffreyi, P. × schwerinii, and one spruce host P. abies are new hosts species of D. pini. P. densiflora and Picea pungens have earlier been reported to be susceptible for DNB. In this study, D. septosporum was found from both tree species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close The occurrence and distribution of Dothistroma needle blight (DNB) were studied in 2014–2017 around Slovakia. A total of 84 localities, both native and planted, were investigated, and the presence of DNB was confirmed in 73 of them. In all positive locations, symptoms typical of DNB were observed and the Dothistroma species was confirmed using species-specific primers either from fungal cultures or directly from needles. Both Dothistroma species—D. septosporum and D. pini—were identified. Both species occurred together in 29 locations, only D. septosporum in 42 and only D. pini in two locations. The host range of D. septosporum included 10 pine species and two spruce species. The host range of D. pini comprised the same number of pine hosts but only one spruce species. Five pine hosts, P. aristata, P. coulteri, P. densiflora, P. jeffreyi, P. × schwerinii, and one spruce host P. abies are new hosts species of D. pini. P. densiflora and Picea pungens have earlier been reported to be susceptible for DNB. In this study, D. septosporum was found from both tree species. Close https://onlinelibrary.wiley.com/doi/abs/10.1111/efp.12421 doi:10.1111/efp.12421 Close
2017
	Ondrušková, E; Hečková, Z; Kádasi-Horáková, M; Koltay, A; Ostrovský, R; Pažitný, J; Adamčíková, K Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia Journal Article European Journal of Plant Pathology, 148 (2), pp. 283-294, 2017, ISSN: 0929-1873. Abstract | Links | BibTeX @article{Ondrušková2017, title = {Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia}, author = {E. Ondrušková and Z. Hečková and M. Kádasi-Horáková and A. Koltay and R. Ostrovský and J. Pažitný and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s10658-016-1088-2}, doi = {10.1007/s10658-016-1088-2}, issn = {0929-1873}, year = {2017}, date = {2017-01-01}, journal = {European Journal of Plant Pathology}, volume = {148}, number = {2}, pages = {283-294}, abstract = {The occurrence and distribution of Dothistroma needle blight (DNB) on Pinus mugo was studied in 2014–2015 around the Slovakia. In total, 42 localities were investigated both native and planted ones. Symptoms of DNB were observed on 35 localities only on planted shrubs. All these 35 localities are new P. mugo DNB stands. No DNB symptoms were observed in natural and naturally regenerated plantations. DNAwas extracted from a total of 236 isolates and eight needle samples. Based on the ITS-rDNA comparisons and using species specific primers, both pathogenic Dothistroma species were detected: D. septosporum and D. pini. Isolates of D. septosporum had ITS sequences identical to D. septosporum from Europe and both mating types were identified with slight predominance of MAT2. The ratio of D. septosporum mating types varies significantly between sites, ranging from an equal proportion of each mating type to single mating type populations. D. pini ITS sequence grouped with D. pini from Ukraine, Russia and Switzerland and only MAT2 was found.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close The occurrence and distribution of Dothistroma needle blight (DNB) on Pinus mugo was studied in 2014–2015 around the Slovakia. In total, 42 localities were investigated both native and planted ones. Symptoms of DNB were observed on 35 localities only on planted shrubs. All these 35 localities are new P. mugo DNB stands. No DNB symptoms were observed in natural and naturally regenerated plantations. DNAwas extracted from a total of 236 isolates and eight needle samples. Based on the ITS-rDNA comparisons and using species specific primers, both pathogenic Dothistroma species were detected: D. septosporum and D. pini. Isolates of D. septosporum had ITS sequences identical to D. septosporum from Europe and both mating types were identified with slight predominance of MAT2. The ratio of D. septosporum mating types varies significantly between sites, ranging from an equal proportion of each mating type to single mating type populations. D. pini ITS sequence grouped with D. pini from Ukraine, Russia and Switzerland and only MAT2 was found. Close https://link.springer.com/article/10.1007/s10658-016-1088-2 doi:10.1007/s10658-016-1088-2 Close
2015
	Alenezi, F N; Fraser, S; Bełka, M; Doğmuş, T H; Heckova, Z; Oskay, F; Belbahri, L; Woodward, S Biological control of Dothistroma needle blight on pine with Aneurinibacillus migulanus Journal Article Forest Pathology : Journal de pathologie forestiere, 46 (5), pp. 145-224, 2015, ISBN: 1437-4781. Abstract | Links | BibTeX @article{Alenezi2015, title = {Biological control of Dothistroma needle blight on pine with \textit{Aneurinibacillus migulanus}}, author = {F. N. Alenezi and S. Fraser and M. Bełka and T.H. Doğmuş and Z. Heckova and F. Oskay and L. Belbahri and S. Woodward}, doi = {10.1111/efp.12237}, isbn = {1437-4781}, year = {2015}, date = {2015-10-29}, journal = {Forest Pathology : Journal de pathologie forestiere}, volume = {46}, number = {5}, pages = {145-224}, abstract = {The ability of two strains of the antibiotic‐producing bacillus Aneurinibacillus migulanus to control Dothistroma septosporum infection of pine was tested in artificial inoculation experiments on 2‐year‐old Pinus contorta plants. Disease severity (% needles with conidiomata) on control plants was 5.8 ± 1.7%, compared with 6.2 ± 2.0% on plants treated with A. migulanus NCTC 7096. In contrast, treatment with A. migulanus Nagano reduced Dothistroma needle blight (DNB) severity to 1.1 ± 0.4%. The conidial density and percentage germination of D. septosporum on needles were also greatly reduced on seedlings treated with A. migulanus Nagano compared with the A. migulanus NCTC 7096 and control treatments. The results suggest that A. migulanus Nagano has potential as a biological control agent for use against DNB in forest nurseries.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Close The ability of two strains of the antibiotic‐producing bacillus Aneurinibacillus migulanus to control Dothistroma septosporum infection of pine was tested in artificial inoculation experiments on 2‐year‐old Pinus contorta plants. Disease severity (% needles with conidiomata) on control plants was 5.8 ± 1.7%, compared with 6.2 ± 2.0% on plants treated with A. migulanus NCTC 7096. In contrast, treatment with A. migulanus Nagano reduced Dothistroma needle blight (DNB) severity to 1.1 ± 0.4%. The conidial density and percentage germination of D. septosporum on needles were also greatly reduced on seedlings treated with A. migulanus Nagano compared with the A. migulanus NCTC 7096 and control treatments. The results suggest that A. migulanus Nagano has potential as a biological control agent for use against DNB in forest nurseries. Close doi:10.1111/efp.12237 Close