Adamčíková Katarína
Plant Pathology and Mycology RG
Research Group Leader
Mgr. Katarína Adamčíková, PhD.
Slovak Academy of Sciences
Institute of Forest Ecology
Plant Pathology & Mycology Research Group
Akademická 2
949 01 Nitra
Phone: +421 37 6943 339
Email: katarina.adamcikova@ife.sk
Research Interests: fungal diseases of woody plants, biological control, mycology, diversity and distribution of pathogens, plant protection. Current research interest: chestnut blight disease, Dothistroma needle blight, ash dieback.
Education:
- PhD., Faculty of Agrobiology and Food Resources Slovak University of Agriculture in Nitra, Slovakia 2004 (plant protection)
- MSc., Faculty of Natural Sciences Comenius University Bratislava, Department of Plant Physiology, Slovak Republic 1997 (biology – plant physiology)
Academic employment:
- Institute of Forest Ecology of Slovak Academy of Sciences
- Senior researcher (since 2013)
- Head of Branch for Woody Plants Biology (since 2012)
- Scientific researcher – (2003 – 2012)
- PhD. student (1997 – 2003)
National projects:
- MVTS Ochrana mestských stromov (2021-2025, member of research team)
- VEGA 2/0132/22 Impact of climate change on the distribution of selected pathogens of Pinus sp. trees (2022-2025, deputy of project leader)
- VEGA 2/0050/22 Unraveling of functional diversity and ecological role of fungi of the family Clavariaceae (2022-2025, member of research team)
- VEGA 1/0346/22 What we (do not) know about the genus Crepidotus (Agaricomycotina, fungi), (2022-2025, member of research team)
- APVV-20-0257 Tree and country – influence of trees on diversity of soil microorganisms in agricultural land (2021-2025, member of research team)
- VEGA 2/0077/18 Identification, genetic variability a pathogenicity of economically important needle cast species on pines (2018-2021, project leader)
- VEGA 2/0062/18 Ash dieback: the causal agents and disease control strategy (2018-2021, member of research team)
- SK-FR-2017-0025 Spatial analyses in population genetic studies of tree pathogens (2018-2019, project leader)
- VEGA 2/0018/18 Systematics and phylogeny of the genus Dermoloma in Europe and North America (2018-2021, member of research team)
- APVV-15-0210 Distribution potential of different fungal groups in Europe (2016–2020, member of research team)
- VEGA 2/00143/15 Study of the effect of environmental variables on the incidence and spread of chestnut blight in European chestnut (Castanea sativa) in Slovakia and possibilities to control this disease (2015-2018, deputy of project leader)
- VEGA 2/0069/14 Biology, spread and diagnostics of harmful agent Dothistroma septosporum (Mycosphaerella pini), D. pini and other harmful agents of pines assimilation organs (2014-2017, deputy of project leader)
- VEGA 2/0071/14 Species diversity and biological characteristics of parasitic fungi associated with damage and withering of woody plants (2014-2017, member of research team)
- HUSK/1101/2.2.1/0230 Name of project: Our common natural resource: the European chestnut (2012-2014, project leader)
- APVV SK-MAD 0024-10 Study of genetic resources of selected nut crops in Slovakia and Romania (2011-2012, member of research team)
- VEGA 2/0149/10 Study of morphological, genetical and biological characteristics of selected genera of fungi from Coelomycetes, their pathogenicity and occurrence in different ecological conditions (2010-2013, member of research team)
- VEGA 2/0149/10 Study of the phenotypic and genetic variability and different susceptibility to the fungi diseases European chestnut (Castanea sativa Mill.) (2010-2013, member of research team)
International projects:
- Euphresco 2016-G-223 Lecanosticta – Brown spot disease of pines – spread in European forest ecosystems: impact on pines, predisposing and contributing factors, control (2017-2020, member of research team)
- COST FP 1403 Pine pitch canker – strategies for management of Gibberella circinata in greenhouses and forests (2015-2019)
- COST FP 1103 Fraxinus dieback in Europe: elaborating guidelines and strategies for sustainable management (2012-2016)
- COST FP 1102 Determining invasiveness and risk of Dothistroma (2011-2015)
List of my publications:
2022 |
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 | Jančovičová, S; Adamčíková, K; Caboň, M; Adamčík, S Phylogeny of Crepidotus applanatus Look-Alikes Reveals a Convergent Morphology Evolution and a New Species C. pini Journal Article Journal of Fungi, 8 (489), 2022, ISSN: 2309-608X. @article{Jančovičová2022, title = {Phylogeny of Crepidotus applanatus Look-Alikes Reveals a Convergent Morphology Evolution and a New Species C. pini}, author = {S. Jančovičová and K. Adamčíková and M. Caboň and S. Adamčík}, url = {http://ife.sk/wp-content/uploads/2020/09/figure-2-5.jpg}, doi = {doi.org/10.3390/jof8050489}, issn = { 2309-608X}, year = {2022}, date = {2022-05-07}, journal = {Journal of Fungi}, volume = {8}, number = {489}, abstract = {Crepidotus applanatus is known as a common wood inhabiting fungus widely distributed throughout the Northern Hemisphere. There have been contrasting opinions about the delimitation and taxonomic treatment of the similar species C. malachius. Our phylogeny did not support the close relationship of these two morphologically similar species and the grouping of collections labelled by both names within each phylogenetic species reflects unreliable species delimitations in the traditional literatures. Both species inhabit the wood of deciduous trees, and our morphological analysis identified the size of basidiospores as a significant difference between them. The collections from Pinus sylvestris are recognised as a new species, C. pini sp. nov., and its morphological identification requires a combination of both basidiospore and cheilocystidia characters.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Crepidotus applanatus is known as a common wood inhabiting fungus widely distributed throughout the Northern Hemisphere. There have been contrasting opinions about the delimitation and taxonomic treatment of the similar species C. malachius. Our phylogeny did not support the close relationship of these two morphologically similar species and the grouping of collections labelled by both names within each phylogenetic species reflects unreliable species delimitations in the traditional literatures. Both species inhabit the wood of deciduous trees, and our morphological analysis identified the size of basidiospores as a significant difference between them. The collections from Pinus sylvestris are recognised as a new species, C. pini sp. nov., and its morphological identification requires a combination of both basidiospore and cheilocystidia characters. |
 | Buyck, B; Eyssartier, G; Armada, F; Corrales, A; Hembrom, M E; Rossi, W; Bellanger, J M; Das, K; Dima, B; Ghosh, A; Noordeloos, M E; Parihar, A; Krisai-Greilhuber, I; M., Leonardi M; Manz, C; Vera, M; Vila, J; Adamčíková, K; Bizio, E; Caboň, M; Hampe, F; Piepenbring, M; Adamčík, S Fungal Biodiversity Profiles 111-120 Journal Article Cryptogamie. Mycologie, 43 (2), pp. 23-61, 2022, ISSN: 1776-100. @article{Buyck2022, title = {Fungal Biodiversity Profiles 111-120}, author = {B. Buyck and G. Eyssartier and F. Armada and A. Corrales and M.E. Hembrom and W. Rossi and J.M. Bellanger and K. Das and B. Dima and A. Ghosh and M.E. Noordeloos and A. Parihar and I. Krisai-Greilhuber and M. Leonardi M. and C. Manz and M. Vera and J. Vila and K. Adamčíková and E. Bizio and M. Caboň and F. Hampe and M. Piepenbring and S. Adamčík }, url = {https://doi.org/10.5252/cryptogamie-mycologie2022v43a2. http://cryptogamie.com/mycologie/43/2}, doi = {doi.org/10.5252/cryptogamie-mycologie2022v43a2. }, issn = { 1776-100}, year = {2022}, date = {2022-03-03}, journal = {Cryptogamie. Mycologie}, volume = {43}, number = {2}, pages = {23-61}, abstract = {In this new series of Fungal Biodiversity Profiles, the authors provide descriptions for ten new taxa: Ilytheomyces uncinatus W. Rossi & M. Leonardi, sp. nov. (Ascomycota, Laboulbeniales) and several Basidiomycota: in family Entolomataceae for Entoloma aurantioalpinum Armada, Vila, Bellanger, Noordel., Krisai & Dima, sp. nov. and E. nigroflavescens Armada, Bellanger, Noordel. & Dima, sp. nov., both from France; in family Inocybaceae for Inocybe hebes Eyssart. & Buyck, sp. nov., I. media Eyssart. & Buyck, sp. nov. and I. leucophaea Eyssart. & Buyck, sp. nov., all three gathered from miombo woodland in Zambia (Africa); in family Russulaceae, Russula ferruginea subsp. ferruginea Corrales & Vera, sp. nov. and subsp. panamanensis Corrales & Manz, subsp. nov. from Central America; in Laetiporaceae Jülich for the Indian Phaeolus sharmae Hembrom, A. Parihar, K. Das & A. Ghosh, sp. nov.; in family Vuilleminiaceae Maire for Vuilleminia tropica Hembrom, A. Ghosh, A. Parihar & K. Das, sp. nov., equally from India. All new species of Basidiomycota are supported by newly provided sequence data or already published phylogenetic analyses of sequence data.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In this new series of Fungal Biodiversity Profiles, the authors provide descriptions for ten new taxa: Ilytheomyces uncinatus W. Rossi & M. Leonardi, sp. nov. (Ascomycota, Laboulbeniales) and several Basidiomycota: in family Entolomataceae for Entoloma aurantioalpinum Armada, Vila, Bellanger, Noordel., Krisai & Dima, sp. nov. and E. nigroflavescens Armada, Bellanger, Noordel. & Dima, sp. nov., both from France; in family Inocybaceae for Inocybe hebes Eyssart. & Buyck, sp. nov., I. media Eyssart. & Buyck, sp. nov. and I. leucophaea Eyssart. & Buyck, sp. nov., all three gathered from miombo woodland in Zambia (Africa); in family Russulaceae, Russula ferruginea subsp. ferruginea Corrales & Vera, sp. nov. and subsp. panamanensis Corrales & Manz, subsp. nov. from Central America; in Laetiporaceae Jülich for the Indian Phaeolus sharmae Hembrom, A. Parihar, K. Das & A. Ghosh, sp. nov.; in family Vuilleminiaceae Maire for Vuilleminia tropica Hembrom, A. Ghosh, A. Parihar & K. Das, sp. nov., equally from India. All new species of Basidiomycota are supported by newly provided sequence data or already published phylogenetic analyses of sequence data. |
 | Kobza, M; Ostrovský, R; Adamčíková, K; Pastirčáková, K Stability of trees infected by wood decay fungi estimated by acoustic tomography: a field survey Journal Article Trees Structure and Function, 36 (1), pp. 103-112, 2022, ISSN: 1432-2285. @article{Kobza2022, title = {Stability of trees infected by wood decay fungi estimated by acoustic tomography: a field survey}, author = {M. Kobza and R. Ostrovský and K. Adamčíková and K. Pastirčáková}, url = {https://doi.org/10.1007/s00468-021-02185-w}, doi = {10.1007/s00468-021-02185-w}, issn = {1432-2285}, year = {2022}, date = {2022-02-08}, journal = {Trees Structure and Function}, volume = {36}, number = {1}, pages = {103-112}, abstract = {In this survey, we assessed the relationships between the presence of visible fruiting bodies and measurements of both wood damage and tree safety factor (SF), the final aim being the determination of whether a tree showing wood decay signs should be felled or not. In general, trees affected by wood-decaying fungi cannot be considered risky prior to determining the impact of the fungi on their structural integrity. Trees of nine genera from 13 localities of Slovakia were included in this survey. In total, nineteen species of fungi were found causing wood decay on 74 individual host trees. Acoustic tomography was done using a Fakopp 3D tomograph, and SF was calculated by ArborSonic 3D software. The most commonly occurring taxon, Fomes fomentarius (L.) Fr., was found on six tree genera. The highest degree of damaged area on trunks was caused by fungi in the genera Fomes, Ganoderma, and Perenniporia (over 70%), while the lowest damage was caused by genera Spongipellis, Cerrena, and Auricularia (up to 30%). The lowest values of SF were measured on trees of the genus Acer infected by Fomes fomentarius; Prunus infected by Phellinus pomaceus (Pers.) Maire; and Fagus infected by Ganoderma pfeifferi Bres. Computation of the SF of individual trees plays a vital part in the stability evaluation of trees affected by wood decay fungi.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In this survey, we assessed the relationships between the presence of visible fruiting bodies and measurements of both wood damage and tree safety factor (SF), the final aim being the determination of whether a tree showing wood decay signs should be felled or not. In general, trees affected by wood-decaying fungi cannot be considered risky prior to determining the impact of the fungi on their structural integrity. Trees of nine genera from 13 localities of Slovakia were included in this survey. In total, nineteen species of fungi were found causing wood decay on 74 individual host trees. Acoustic tomography was done using a Fakopp 3D tomograph, and SF was calculated by ArborSonic 3D software. The most commonly occurring taxon, Fomes fomentarius (L.) Fr., was found on six tree genera. The highest degree of damaged area on trunks was caused by fungi in the genera Fomes, Ganoderma, and Perenniporia (over 70%), while the lowest damage was caused by genera Spongipellis, Cerrena, and Auricularia (up to 30%). The lowest values of SF were measured on trees of the genus Acer infected by Fomes fomentarius; Prunus infected by Phellinus pomaceus (Pers.) Maire; and Fagus infected by Ganoderma pfeifferi Bres. Computation of the SF of individual trees plays a vital part in the stability evaluation of trees affected by wood decay fungi. |
 | Kádasi-Horáková, M; Barta, M; Adamčíková, K; Ostrovský, R; Pastirčáková, K Hymenoscyphus fraxineus on Fraxinus excelsior in Slovakia: distribution and mating types Journal Article Biologia, 2022, ISSN: 0006-3088. @article{Kádasi-Horáková2022, title = {\textit{Hymenoscyphus fraxineus} on \textit{Fraxinus excelsior} in Slovakia: distribution and mating types}, author = {M. Kádasi-Horáková and M. Barta and K. Adamčíková and R. Ostrovský and K. Pastirčáková}, doi = {10.1007/s11756-022-01023-9}, issn = {0006-3088}, year = {2022}, date = {2022-02-08}, journal = {Biologia}, abstract = {Hymenoscyphus fraxineus causes ash dieback in Europe and threatens the future existence of Fraxinus excelsior in large parts of its natural distribution range. In this study, we report the first documented distribution of the pathogen on the most common ash species F. excelsior in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region of rDNA. Analysis of the mating type genes of H. fraxineus isolates revealed the presence of both mating types in Slovakia. Hymenoscyphus fraxineus-positive trees were recorded in 70 localities with different types of management in different parts of the country, mainly in forest stands. The results indicate the widespread distribution of H. fraxineus across the entire country, wherever the host is present.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Hymenoscyphus fraxineus causes ash dieback in Europe and threatens the future existence of Fraxinus excelsior in large parts of its natural distribution range. In this study, we report the first documented distribution of the pathogen on the most common ash species F. excelsior in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region of rDNA. Analysis of the mating type genes of H. fraxineus isolates revealed the presence of both mating types in Slovakia. Hymenoscyphus fraxineus-positive trees were recorded in 70 localities with different types of management in different parts of the country, mainly in forest stands. The results indicate the widespread distribution of H. fraxineus across the entire country, wherever the host is present. |
2021 |
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 | Manz, C; Adamčík, S; Looney, B P; Corrales, A; Ovrebo, C; Adamčíková, K; Hofmann, T A; Hampe, F; Piepenbring, M Four new species of Russula subsection Roseinae from tropical montane forests in western Panama Journal Article PLoS ONE, 16 (10), pp. e0257616, 2021, ISSN: 1932-6203. @article{Manz2021, title = {Four new species of Russula subsection Roseinae from tropical montane forests in western Panama}, author = {C. Manz and S. Adamčík and B. P. Looney and A. Corrales and C. Ovrebo and K. Adamčíková and T. A. Hofmann and F. Hampe and M. Piepenbring }, url = {https://doi.org/10.1371/journal.pone.0257616}, doi = {doi.org/10.1371/journal.pone.0257616}, issn = {1932-6203}, year = {2021}, date = {2021-10-14}, journal = {PLoS ONE}, volume = {16}, number = {10}, pages = {e0257616}, abstract = {Species of the genus Russula are key components of ectomycorrhizal ecosystems worldwide. Nevertheless, their diversity in the tropics is still poorly known. This study aims to contribute to the knowledge of the diversity of Russula species classified in subsection Roseinae based on specimens recently collected in tropical montane rainforests in western Panama. A five gene multilocus phylogeny based on the nuclear markers ITS nrDNA, MCM7, RPB1, RPB2 and TEF-1α was constructed to identify the systematic position of 22 collections from Panama. Four new species, Russula cornicolor, Russula cynorhodon, Russula oreomunneae and Russula zephyrovelutipes are formally described and illustrated. None of the four species are sister species and they are more closely related to North American or Asian species. Two of the newly described species were associated with the ectomycorrhizal tree species Oreomunnea mexicana, while the other two species were associated with Quercus species. All four species are so far only known from mountains in western Panama.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species of the genus Russula are key components of ectomycorrhizal ecosystems worldwide. Nevertheless, their diversity in the tropics is still poorly known. This study aims to contribute to the knowledge of the diversity of Russula species classified in subsection Roseinae based on specimens recently collected in tropical montane rainforests in western Panama. A five gene multilocus phylogeny based on the nuclear markers ITS nrDNA, MCM7, RPB1, RPB2 and TEF-1α was constructed to identify the systematic position of 22 collections from Panama. Four new species, Russula cornicolor, Russula cynorhodon, Russula oreomunneae and Russula zephyrovelutipes are formally described and illustrated. None of the four species are sister species and they are more closely related to North American or Asian species. Two of the newly described species were associated with the ectomycorrhizal tree species Oreomunnea mexicana, while the other two species were associated with Quercus species. All four species are so far only known from mountains in western Panama. |
 | Lange, De R; Adamčík, S; Adamčíková, K; Asselman, P; Borovička, J; Delgat, L; Hampe, F; Verbeken, A Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex Journal Article IMA Fungus, 20 (Article ID 12), pp. 1-31, 2021, ISSN: 2210-6359. @article{Lange2020, title = {Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex}, author = {R. De Lange and S. Adamčík and K. Adamčíková and P. Asselman and J. Borovička and L. Delgat and F. Hampe and A. Verbeken}, url = {https://imafungus.biomedcentral.com/articles/10.1186/s43008-021-00064-0}, doi = {10.1186/s43008-021-00064-0}, issn = {2210-6359}, year = {2021}, date = {2021-08-02}, journal = {IMA Fungus}, volume = {20}, number = {Article ID 12}, pages = {1-31}, abstract = {Russula albonigra is considered a well-known species, morphologically delimited by the context of the basidiomata blackening without intermediate reddening, and the menthol-cooling taste of the lamellae. It is supposed to have a broad ecological range and a large distribution area. A thorough molecular analysis based on four nuclear markers (ITS, LSU, RPB2 and TEF1-α) shows this traditional concept of R. albonigra s. lat. represents a species complex consisting of at least five European, three North American, and one Chinese species. Morphological study shows traditional characters used to delimit R. albonigra are not always reliable. Therefore, a new delimitation of the R. albonigra complex is proposed and a key to the described European species of R. subgen. Compactae is presented. A lectotype and an epitype are designated for R. albonigra and three new European species are described: R. ambusta, R. nigrifacta, and R. ustulata. Different thresholds of UNITE species hypotheses were tested against the taxonomic data. The distance threshold of 0.5% gives a perfect match to the phylogenetically defined species within the R. albonigra complex. Publicly available sequence data can contribute to species delimitation and increase our knowledge on ecology and distribution, but the pitfalls are short and low quality sequences.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Russula albonigra is considered a well-known species, morphologically delimited by the context of the basidiomata blackening without intermediate reddening, and the menthol-cooling taste of the lamellae. It is supposed to have a broad ecological range and a large distribution area. A thorough molecular analysis based on four nuclear markers (ITS, LSU, RPB2 and TEF1-α) shows this traditional concept of R. albonigra s. lat. represents a species complex consisting of at least five European, three North American, and one Chinese species. Morphological study shows traditional characters used to delimit R. albonigra are not always reliable. Therefore, a new delimitation of the R. albonigra complex is proposed and a key to the described European species of R. subgen. Compactae is presented. A lectotype and an epitype are designated for R. albonigra and three new European species are described: R. ambusta, R. nigrifacta, and R. ustulata. Different thresholds of UNITE species hypotheses were tested against the taxonomic data. The distance threshold of 0.5% gives a perfect match to the phylogenetically defined species within the R. albonigra complex. Publicly available sequence data can contribute to species delimitation and increase our knowledge on ecology and distribution, but the pitfalls are short and low quality sequences. |
 | Adamčíková, K; Jánošíková, Z; Adamčík, S; Ostrovský, R; Pastirčáková, K; Kobza, M; Ondrušková, E Host range, genetic variability, and mating types of Lecanosticta acicola in Slovakia Journal Article Scandinavian Journal of Forest Research, 36 (5), pp. 325-332, 2021, ISSN: 0282-7581. @article{Adamčíková2021b, title = {Host range, genetic variability, and mating types of \textit{Lecanosticta acicola} in Slovakia}, author = {K. Adamčíková and Z. Jánošíková and S. Adamčík and R. Ostrovský and K. Pastirčáková and M. Kobza and E. Ondrušková}, url = {https://doi.org/10.1080/02827581.2021.1941236}, doi = {10.1080/02827581.2021.1941236}, issn = {0282-7581}, year = {2021}, date = {2021-07-16}, journal = {Scandinavian Journal of Forest Research}, volume = {36}, number = {5}, pages = {325-332}, abstract = {In recent years, there has been an apparent Europe-wide emerging presence of brown spot needle blight, a disease of pine species caused by the fungus Lecanosticta acicola. In this study, we report the first well-documented occurrences of the pathogen in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region and TEF1 gene. Among the material collected from 84 locations within the country, L. acicola was present in 17 samples from 13 different locations, mainly distributed in urban environments. Four pine species were identified as hosts, among which, Pinus nigra and P. mugo were found to be the most frequently infected. Analysis of the mating type genes of 24 isolates obtained from two localities revealed the presence of a single mating type in Slovakia. All analyzed ITS sequences of the Slovak isolates were found to be uniform. However, although analysis of the TEF1 gene indicated that all Slovak isolates could be grouped into a single lineage, we detected nucleotide polymorphisms suggestive of a certain degree of genetic diversification within central European populations of the fungus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In recent years, there has been an apparent Europe-wide emerging presence of brown spot needle blight, a disease of pine species caused by the fungus Lecanosticta acicola. In this study, we report the first well-documented occurrences of the pathogen in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region and TEF1 gene. Among the material collected from 84 locations within the country, L. acicola was present in 17 samples from 13 different locations, mainly distributed in urban environments. Four pine species were identified as hosts, among which, Pinus nigra and P. mugo were found to be the most frequently infected. Analysis of the mating type genes of 24 isolates obtained from two localities revealed the presence of a single mating type in Slovakia. All analyzed ITS sequences of the Slovak isolates were found to be uniform. However, although analysis of the TEF1 gene indicated that all Slovak isolates could be grouped into a single lineage, we detected nucleotide polymorphisms suggestive of a certain degree of genetic diversification within central European populations of the fungus. |
 | Adamčíková, K; Jánošíková, Z; van der Nest, A; Adamčík, S; Ondrušková, E; Barnes, I Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia Journal Article Plant Pathology, 70 (8), pp. 1883-1896, 2021, ISSN: 1365-3059. @article{Adamčíková2021c, title = {Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia}, author = {K. Adamčíková and Z. Jánošíková and A. van der Nest and S. Adamčík and E. Ondrušková and I. Barnes }, url = {https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13428}, doi = {10.1111/ppa.13428}, issn = {1365-3059}, year = {2021}, date = {2021-06-30}, journal = {Plant Pathology}, volume = {70}, number = {8}, pages = {1883-1896}, abstract = {Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia. |
 | Jánošíková, Z; Dutech, C; Ondrušková, E; Adamčíková, K; Mullett, M Population structure and genetic diversity of Dothistroma septosporum in Slovakia Journal Article European Journal of Plant Pathology, 160 (4), pp. 771-787, 2021, ISSN: 1573-8469. @article{Jánošíková2021, title = {Population structure and genetic diversity of Dothistroma septosporum in Slovakia}, author = {Z. Jánošíková and C. Dutech and E. Ondrušková and K. Adamčíková and M. Mullett}, url = {https://link.springer.com/article/10.1007/s10658-021-02266-z}, doi = {doi:10.1007/s10658-021-02266-z}, issn = {1573-8469}, year = {2021}, date = {2021-06-23}, journal = {European Journal of Plant Pathology}, volume = {160}, number = {4}, pages = {771-787}, abstract = {Dothistroma needle blight (DNB) is a serious pine disease present worldwide caused by the ascomycetous fungi Dothistroma septosporum and Dothistroma pini. Based on multiplex PCR analysis of 11 microsatellite markers screened on 253 D. septosporum isolates obtained from 32 sites across Slovakia, a total of 137 unique multilocus haplotypes (MLHs) were detected. The majority of MLHs (n = 91) were represented by a single isolate, but 13 MLHs were found at two different sites, and in some cases, these were separated by large distances. Four population clusters were identified using a discriminant analysis of principal components (DAPC). The genetic clusters obtained from the DAPC were mixed throughout the country and were not restricted to particular host species. Although both mating types of D. septosporum were found to be in an almost perfect 1:1 ratio in both the non-clone-corrected and clone-corrected datasets, random mating was rejected in the entire dataset. Random mating was only supported in the Pinus mugo group planted in urban areas and on a smaller spatial scale in two sites (Kálnica and Litava) using the clonecorrected dataset. These findings suggest a mixed mode of reproduction with an important component of sexual reproduction, although the sexual stage of the fungus (teleomorph) has not been physically observed in Slovakia yet. The examination of spatial relationships using spatial principal component analyses and the presence of isolation-by-distance together with relatively high genetic diversity suggests the pathogen has been long established in Slovakia and spread naturally across the landscape. However, the weak population structure and findings of identical clones at widely separated sites strongly suggests some degree of human assisted dispersal.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a serious pine disease present worldwide caused by the ascomycetous fungi Dothistroma septosporum and Dothistroma pini. Based on multiplex PCR analysis of 11 microsatellite markers screened on 253 D. septosporum isolates obtained from 32 sites across Slovakia, a total of 137 unique multilocus haplotypes (MLHs) were detected. The majority of MLHs (n = 91) were represented by a single isolate, but 13 MLHs were found at two different sites, and in some cases, these were separated by large distances. Four population clusters were identified using a discriminant analysis of principal components (DAPC). The genetic clusters obtained from the DAPC were mixed throughout the country and were not restricted to particular host species. Although both mating types of D. septosporum were found to be in an almost perfect 1:1 ratio in both the non-clone-corrected and clone-corrected datasets, random mating was rejected in the entire dataset. Random mating was only supported in the Pinus mugo group planted in urban areas and on a smaller spatial scale in two sites (Kálnica and Litava) using the clonecorrected dataset. These findings suggest a mixed mode of reproduction with an important component of sexual reproduction, although the sexual stage of the fungus (teleomorph) has not been physically observed in Slovakia yet. The examination of spatial relationships using spatial principal component analyses and the presence of isolation-by-distance together with relatively high genetic diversity suggests the pathogen has been long established in Slovakia and spread naturally across the landscape. However, the weak population structure and findings of identical clones at widely separated sites strongly suggests some degree of human assisted dispersal. |
 | Vera, M; Adamčík, S; Adamčíková, K; Hampe, F; Caboň, M; Manz, C; Overbo, C; Peipenbring, M; Corrales, A Morphological and genetic diversification of Russula floriformis, sp. nov., along the Isthmus of Panama Journal Article Mycologia, 113 (4), pp. 807-827, 2021, ISSN: 1557-2536. @article{Vera2021, title = {Morphological and genetic diversification of Russula floriformis, sp. nov., along the Isthmus of Panama}, author = {M. Vera and S. Adamčík and K. Adamčíková and F. Hampe and M. Caboň and C. Manz and C. Overbo and M. Peipenbring and A. Corrales}, url = {https://www.tandfonline.com/doi/full/10.1080/00275514.2021.1897377}, doi = {DOI: 10.1080/00275514.2021.1897377}, issn = {1557-2536}, year = {2021}, date = {2021-05-27}, journal = {Mycologia}, volume = {113}, number = {4}, pages = {807-827}, abstract = {Species of Russula are ubiquitous members of ectomycorrhizal fungal communities in tropical ecosystems. However, an important part of the total tropical diversity of this genus and its biogeographic patterns is unknown due to the lack of studies on Russula in tropical ecosystems. We combined molecular, morphological, ecological, and biogeographic data to elaborate concepts for two new subspecies of R. floriformis (subsection Substriatinae). Russula floriformis subsp. floriformis and R. floriformis subsp. symphoniae are described as new from montane forest dominated by Quercus and/or Oreomunnea (Fagales) from Colombia and Panama, respectively. Phylogenies were constructed using nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), D1–D2 domains of nuc 28S rDNA (28S), and partial regions of the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-alpha (tef1). Similar environmental conditions, similar morphology, and an ITS sequence similarity higher than 99% with only three different positions indicate that these two subspecies are closely related. Detailed observations of microscopic structures and analyses of further DNA loci, however, revealed morphological and molecular characteristics that allow distinguishing the two subspecies of R. floriformis. Spatial distribution and phylogenetic proximity of the two Russula subspecies and their ectomycorrhizal hosts, i.e., species of Quercus, suggest that their diversification is a result of comigration, adaptation, and geographic isolation along the Isthmus of Panama during the Pliocene and Pleistocene.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species of Russula are ubiquitous members of ectomycorrhizal fungal communities in tropical ecosystems. However, an important part of the total tropical diversity of this genus and its biogeographic patterns is unknown due to the lack of studies on Russula in tropical ecosystems. We combined molecular, morphological, ecological, and biogeographic data to elaborate concepts for two new subspecies of R. floriformis (subsection Substriatinae). Russula floriformis subsp. floriformis and R. floriformis subsp. symphoniae are described as new from montane forest dominated by Quercus and/or Oreomunnea (Fagales) from Colombia and Panama, respectively. Phylogenies were constructed using nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), D1–D2 domains of nuc 28S rDNA (28S), and partial regions of the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-alpha (tef1). Similar environmental conditions, similar morphology, and an ITS sequence similarity higher than 99% with only three different positions indicate that these two subspecies are closely related. Detailed observations of microscopic structures and analyses of further DNA loci, however, revealed morphological and molecular characteristics that allow distinguishing the two subspecies of R. floriformis. Spatial distribution and phylogenetic proximity of the two Russula subspecies and their ectomycorrhizal hosts, i.e., species of Quercus, suggest that their diversification is a result of comigration, adaptation, and geographic isolation along the Isthmus of Panama during the Pliocene and Pleistocene. |
 | Adamčíková, K; Ondrušková, E; Pažitný, J; Jánošíková, Z Divergence in culture morphology between two related species, Dothistroma septosporum and D. pini Journal Article Czech Mycology, 73 (1), pp. 109-119, 2021, ISSN: 1805-1421. @article{Adamčíková2021, title = {Divergence in culture morphology between two related species, Dothistroma septosporum and D. pini}, author = {K. Adamčíková and E. Ondrušková and J. Pažitný and Z. Jánošíková}, url = {http://ife.sk/wp-content/uploads/2020/09/fig.-2-rev-5.tif}, doi = {doi.org/10.33585/cmy.73108}, issn = {1805-1421}, year = {2021}, date = {2021-05-24}, journal = {Czech Mycology}, volume = {73}, number = {1}, pages = {109-119}, abstract = {Dothistroma needle blight (DNB) is one of the most important pine needle diseases worldwide. The disease is caused by two fungal species, Dothistroma septosporum and D. pini, which are similar not only in terms of their ecology, causing similar symptoms in hosts of the genus Pinus, but also in terms of their morphological characteristics. The morphologies of 353 cultured Dothistroma isolates from 11 different pine species in Slovakia and their relation to Dothistroma species and/or fungus origin (host) were studied and evaluated by means of the Chi-square test. The cultures were classified into eight categories according to pigmentation. Dothistroma pini isolates were assigned to 4 of the 8 categories, and D. septosporum isolates were assigned to all 8 categories with varying frequencies. The Chi-square test revealed that the culture morphology categories of D. pini and D. septosporum differed significantly. Interactions between the evaluated factors (culture morphology, Dothistroma and host species) were analysed and showed significant differences.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is one of the most important pine needle diseases worldwide. The disease is caused by two fungal species, Dothistroma septosporum and D. pini, which are similar not only in terms of their ecology, causing similar symptoms in hosts of the genus Pinus, but also in terms of their morphological characteristics. The morphologies of 353 cultured Dothistroma isolates from 11 different pine species in Slovakia and their relation to Dothistroma species and/or fungus origin (host) were studied and evaluated by means of the Chi-square test. The cultures were classified into eight categories according to pigmentation. Dothistroma pini isolates were assigned to 4 of the 8 categories, and D. septosporum isolates were assigned to all 8 categories with varying frequencies. The Chi-square test revealed that the culture morphology categories of D. pini and D. septosporum differed significantly. Interactions between the evaluated factors (culture morphology, Dothistroma and host species) were analysed and showed significant differences. |
 | Pastirčáková, K; Adamčík, S; Adamčíková, K; Chater, A O Erysiphe hypophylla, a second powdery mildew (Erysiphales) on oaks in Britain Journal Article Field Mycology, 22 (2), pp. 50-54, 2021, ISSN: 1468-1641. @article{Pastirčáková2021b, title = {\textit{Erysiphe hypophylla}, a second powdery mildew (\textit{Erysiphales}) on oaks in Britain}, author = {K. Pastirčáková and S. Adamčík and K. Adamčíková and A.O. Chater}, doi = {10.1016/j.fldmyc.2021.04.008}, issn = {1468-1641}, year = {2021}, date = {2021-04-20}, journal = {Field Mycology}, volume = {22}, number = {2}, pages = {50-54}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
 | Pastirčáková, K; Adamčíková, K; Bacigálová, K; Caboň, M; Mikušová, P; Senko, D; Svitok, M; Adamčík, S Ash trees (Fraxinus spp.) in urban greenery as possible invasion gates of non-native Phyllactinia species Journal Article Forests, 12 (2), pp. 1-16, Article ID 183, 2021, ISSN: 1999-4907. @article{Pastirčáková2021, title = {Ash trees (\textit{Fraxinus} spp.) in urban greenery as possible invasion gates of non-native \textit{Phyllactinia} species}, author = {K. Pastirčáková and K. Adamčíková and K. Bacigálová and M. Caboň and P. Mikušová and D. Senko and M. Svitok and S. Adamčík}, doi = {10.3390/f12020183}, issn = {1999-4907}, year = {2021}, date = {2021-02-06}, journal = {Forests}, volume = {12}, number = {2}, pages = {1-16, Article ID 183}, abstract = {Two Phyllactinia species have been associated with powdery mildew on leaves of ash trees (Fraxinus) in Eurasia, Phyllactinia fraxinicola U. Braun & H.D. Shin from Southeast Asia and Phyllactinia fraxini (DC.) Fuss from Europe. Non-native ash trees are planted in urban greeneries in both Europe and Southeast Asia, but so far, the two Phyllactinia species have not been reported from the same area. Our molecular analysis of European material consisting of 55 Phyllactinia specimens from 15 countries confirmed the absence of P. fraxinicola in Europe. In Europe, we confirmed P. fraxini on all three European native ash species and on the introduced Asian ash species, Fraxinus chinensis ssp. rhynchophylla (Hance) A.E. Murray and Fraxinus mandshurica Rupr., planted in arboreta. Among the 11 collections examined from Southeast Asia, 3 were identified as P. fraxini and 8 as P. fraxinicola. The environmental niches of the two Phyllactinia species do not show significant overlap in the multidimensional space defined by bioclimatic variables. This suggests that the Asian species P. fraxinicola is not adapted to conditions prevailing in most of Europe and does not represent an invasive threat across the continent. Models of the potential distribution of Phyllactinia species do not overlap in Europe, but there are some areas to the northwest that could be susceptible to invasion by P. fraxinicola.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Two Phyllactinia species have been associated with powdery mildew on leaves of ash trees (Fraxinus) in Eurasia, Phyllactinia fraxinicola U. Braun & H.D. Shin from Southeast Asia and Phyllactinia fraxini (DC.) Fuss from Europe. Non-native ash trees are planted in urban greeneries in both Europe and Southeast Asia, but so far, the two Phyllactinia species have not been reported from the same area. Our molecular analysis of European material consisting of 55 Phyllactinia specimens from 15 countries confirmed the absence of P. fraxinicola in Europe. In Europe, we confirmed P. fraxini on all three European native ash species and on the introduced Asian ash species, Fraxinus chinensis ssp. rhynchophylla (Hance) A.E. Murray and Fraxinus mandshurica Rupr., planted in arboreta. Among the 11 collections examined from Southeast Asia, 3 were identified as P. fraxini and 8 as P. fraxinicola. The environmental niches of the two Phyllactinia species do not show significant overlap in the multidimensional space defined by bioclimatic variables. This suggests that the Asian species P. fraxinicola is not adapted to conditions prevailing in most of Europe and does not represent an invasive threat across the continent. Models of the potential distribution of Phyllactinia species do not overlap in Europe, but there are some areas to the northwest that could be susceptible to invasion by P. fraxinicola. |
 | Mullett, M S; Drenkhan, R; Adamson, K; Boroń, P; Lenart-Boroń, A; Barnes, I; Tomšovský, M; Jánošíková, Z; Adamčíková, K; Ondrušková, E; Queloz, V; Piškur, B; Musolin, D L; Davydenko, K; Georgieva, M; Schmitz, S; Kačergius, A; Ghelardini, L; Orlović, Kranjec J; Müller, M; Oskay, F; Hauptman, T; Halász, Á; Markovskaja, S; Solheim, H; Vuorinen, M; Heinzelmann, R; Hamelin, R C; Konečný, A Journal of Fungi, 7 (2), pp. Article number: 111, 2021, ISBN: 2309-608X. @article{Mullett2021, title = {Worldwide Genetic Structure Elucidates the Eurasian Origin and Invasion Pathways of Dothistroma septosporum, Causal Agent of Dothistroma Needle Blight }, author = {M. S. Mullett and R. Drenkhan and K. Adamson and P. Boroń and A. Lenart-Boroń and I. Barnes and M. Tomšovský and Z. Jánošíková and K. Adamčíková and E. Ondrušková and V. Queloz and B. Piškur and D.L. Musolin and K. Davydenko and M. Georgieva and S. Schmitz and A. Kačergius and L. Ghelardini and J. Kranjec Orlović and M. Müller and F. Oskay and T. Hauptman and Á. Halász and S. Markovskaja and H. Solheim and M. Vuorinen and R. Heinzelmann and R.C. Hamelin and A. Konečný}, doi = {doi 10.3390/jof7020111 }, isbn = {2309-608X}, year = {2021}, date = {2021-02-03}, journal = {Journal of Fungi}, volume = {7}, number = {2}, pages = {Article number: 111}, abstract = {Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia. |
 | Sánchez-García, M; Adamčíková, K; Moreau, P -A; Vizzini, A; Jančovičová, S; Kiran, M; Caboň, M; Matheny, P B; Adamčík, S The genus Dermoloma is more diverse than expected and forms a monophyletic lineage in the Tricholomataceae Journal Article Mycological Progress, 20 (1), pp. 11–25, 2021, ISSN: 1617-416X. @article{Sánchez-García2021, title = {The genus Dermoloma is more diverse than expected and forms a monophyletic lineage in the Tricholomataceae}, author = {M. Sánchez-García and K. Adamčíková and P.-A. Moreau and A. Vizzini and S. Jančovičová and M. Kiran and M. Caboň and P. B. Matheny and S. Adamčík}, doi = {doi 10.1007/s11557-020-01651-y}, issn = {1617-416X}, year = {2021}, date = {2021-01-18}, journal = {Mycological Progress}, volume = {20}, number = {1}, pages = {11–25}, abstract = {We present the first phylogenetic evaluation of the genus Dermoloma, which is resolved as monophyletic and closely related to Pseudotricholoma, a poorly known Dermoloma-like lineage within the family Tricholomataceae. The position of Dermoloma is confirmed by the placement of the type species, Dermoloma cuneifolium, represented by multiple samples including the neotype. Based on our phylogenetic analyses, we recognised 25 European operational taxonomic units (OTUs) but could only assign species names to ten of them based on ex-type sequences. Furthermore, only five additional published Dermoloma names of uncertain status are available for the remaining 16 potential European species, thus demonstrating an unexpected amount of taxonomic diversity. Samples from Europe and North America seem to be endemic on a continental scale. North American samples formed six unique OTUs, but only one could be reliably named, Dermoloma hymenocephalum. Dermoloma is morphologically defined by basidiomata with brown, grey and white colours with a farinaceous odour and a pluristratous hymeniderm type of pileipellis. Our phylogenetic analyses support the subdivision of the genus into two subgenera and four sections, and species with inamyloid basidiospores are placed in subg. Dermoloma and those with amyloid basidiospores in subg. Amylospora. Both subgenera are further divided into two sections. The analysis of spore morphology shows that sect. Conica of subg. Dermoloma and sect. Nigrescentia of subg. Amylospora have a very distinctive spore shape. Sect. Atrobrunnea of subg. Amylospora showed relatively high variability of spores among species, but spores of sect. Dermoloma were similar and not useful for species discrimination.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We present the first phylogenetic evaluation of the genus Dermoloma, which is resolved as monophyletic and closely related to Pseudotricholoma, a poorly known Dermoloma-like lineage within the family Tricholomataceae. The position of Dermoloma is confirmed by the placement of the type species, Dermoloma cuneifolium, represented by multiple samples including the neotype. Based on our phylogenetic analyses, we recognised 25 European operational taxonomic units (OTUs) but could only assign species names to ten of them based on ex-type sequences. Furthermore, only five additional published Dermoloma names of uncertain status are available for the remaining 16 potential European species, thus demonstrating an unexpected amount of taxonomic diversity. Samples from Europe and North America seem to be endemic on a continental scale. North American samples formed six unique OTUs, but only one could be reliably named, Dermoloma hymenocephalum. Dermoloma is morphologically defined by basidiomata with brown, grey and white colours with a farinaceous odour and a pluristratous hymeniderm type of pileipellis. Our phylogenetic analyses support the subdivision of the genus into two subgenera and four sections, and species with inamyloid basidiospores are placed in subg. Dermoloma and those with amyloid basidiospores in subg. Amylospora. Both subgenera are further divided into two sections. The analysis of spore morphology shows that sect. Conica of subg. Dermoloma and sect. Nigrescentia of subg. Amylospora have a very distinctive spore shape. Sect. Atrobrunnea of subg. Amylospora showed relatively high variability of spores among species, but spores of sect. Dermoloma were similar and not useful for species discrimination. |
2020 |
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 | Bičárová, S; Shashikumar, A; Richier, Dalstein- L; Lukasová, V; Adamčíková, K; Pavlendová, H; Sitková, Z; Buchholcerová, A; Bilčík, D The response of Pinus species to ozone uptake in different climate regions of Europe Journal Article Central European Forestry Journal , 66 (4), pp. 255–268, 2020, ISBN: 2454-034X. @article{Bičárová2020, title = {The response of Pinus species to ozone uptake in different climate regions of Europe}, author = {S. Bičárová and A. Shashikumar and L. Dalstein- Richier and V. Lukasová and K. Adamčíková and H. Pavlendová and Z. Sitková and A. Buchholcerová and D. Bilčík}, doi = {DOI: 10.2478/forj-2020-0022}, isbn = {2454-034X}, year = {2020}, date = {2020-12-04}, journal = {Central European Forestry Journal }, volume = {66}, number = {4}, pages = {255–268}, abstract = {This study is focused on the research of selected Pinus species exposed to high ozone concentrations in the mountain environment. We noticed different values of modelled ozone doses (MOD) up-taken by Mountain pine (Pinus mugo Turra) in the High Tatra Mts (SK–HTMts) and Swiss stone pine (Pinus cembra L.) in the Alpes-Mercantour (FR–AlpMar) during the growing season 2019. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. Testing has shown that ozone input based on passive sampling may be used in MOD modelling for sites situated in the subalpine zone where the operation of active monitors is limited. . Presented results confirmed the assumption regarding stomatal ozone flux reduction due to the occurrence of soil drought in hot and dry summer weather typical for the Mediterranean climate region. Despite the limitation of stomatal flux, foliar ozone specific injury on two years needles of P. cembra was substantially higher in comparison to the incidence of ozone injury symptoms observed on two years needles of P. mugo in SK–HTMts. It may suggest low phytotoxicity of given MOD or efficient resistance of P. mugo against oxidative stress. In addition, the visible injury index (VINX) covering the broad effect of biotic and abiotic harmful agents was appraised on P. mugo. Percentage of affected surface indicated moderate deterioration of needle injury at the end of the growing season, particularly due to traces of mechanical damage.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study is focused on the research of selected Pinus species exposed to high ozone concentrations in the mountain environment. We noticed different values of modelled ozone doses (MOD) up-taken by Mountain pine (Pinus mugo Turra) in the High Tatra Mts (SK–HTMts) and Swiss stone pine (Pinus cembra L.) in the Alpes-Mercantour (FR–AlpMar) during the growing season 2019. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. Testing has shown that ozone input based on passive sampling may be used in MOD modelling for sites situated in the subalpine zone where the operation of active monitors is limited. . Presented results confirmed the assumption regarding stomatal ozone flux reduction due to the occurrence of soil drought in hot and dry summer weather typical for the Mediterranean climate region. Despite the limitation of stomatal flux, foliar ozone specific injury on two years needles of P. cembra was substantially higher in comparison to the incidence of ozone injury symptoms observed on two years needles of P. mugo in SK–HTMts. It may suggest low phytotoxicity of given MOD or efficient resistance of P. mugo against oxidative stress. In addition, the visible injury index (VINX) covering the broad effect of biotic and abiotic harmful agents was appraised on P. mugo. Percentage of affected surface indicated moderate deterioration of needle injury at the end of the growing season, particularly due to traces of mechanical damage. |
 | Adamčíková, K; Jánošíková, Z; Pažitný, J Inhibition effect of selected inorganic metal ions on the mycelial growth of Cryphonectria parasitica Journal Article Journal of Plant Protection Research, 60 (4), pp. 399-405, 2020, ISSN: 1427-4345. @article{Adamčíková2020, title = {Inhibition effect of selected inorganic metal ions on the mycelial growth of Cryphonectria parasitica}, author = {K. Adamčíková and Z. Jánošíková and J. Pažitný}, doi = {DOI: 10.24425/jppr.2020.134915}, issn = {1427-4345}, year = {2020}, date = {2020-12-02}, journal = {Journal of Plant Protection Research}, volume = {60}, number = {4}, pages = {399-405}, abstract = {In the current study the antifungal activity of inorganic reagents was tested against Cryphonectria parasitica in vitro in a mycelial growth inhibition test. Three reagents, each consisting of chloride silver (AgCl) in combination with (1) aluminum oxide, (2) zinc oxide − ZnO, and (3) aluminum oxide and titanium dioxide were tested. Significant differences of the tested reagents on the growth of C. parasitica were recorded. The study demonstrated that silver in mixture with ZnO had an antifungal effect and significantly reduced the mycelial growth of C. parasitica in vitro. The mixture of AgCl with the other two combinations of inorganic metal oxides had no inhibition effect on the growth of the pathogen. It was confirmed that ZnO (applied in a single compound test) is responsible for inhibition of C. parasitica mycelium growth. A preliminary in planta assay was performed but statistically significant differences were not recorded in the average increment of canker length.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the current study the antifungal activity of inorganic reagents was tested against Cryphonectria parasitica in vitro in a mycelial growth inhibition test. Three reagents, each consisting of chloride silver (AgCl) in combination with (1) aluminum oxide, (2) zinc oxide − ZnO, and (3) aluminum oxide and titanium dioxide were tested. Significant differences of the tested reagents on the growth of C. parasitica were recorded. The study demonstrated that silver in mixture with ZnO had an antifungal effect and significantly reduced the mycelial growth of C. parasitica in vitro. The mixture of AgCl with the other two combinations of inorganic metal oxides had no inhibition effect on the growth of the pathogen. It was confirmed that ZnO (applied in a single compound test) is responsible for inhibition of C. parasitica mycelium growth. A preliminary in planta assay was performed but statistically significant differences were not recorded in the average increment of canker length. |
 | Ondrušková, Emília; Ostrovský, Radovan; Jánošíková, Zuzana; Adamčíková, Katarína; Kobza, Marek Selected climatic variables in Slovakia are favourable to the development of Dothistroma needle blight Journal Article Folia Oecologica, 47 (2), pp. 144-152, 2020. @article{Ondrušková2020b, title = {Selected climatic variables in Slovakia are favourable to the development of Dothistroma needle blight}, author = {Emília Ondrušková and Radovan Ostrovský and Zuzana Jánošíková and Katarína Adamčíková and Marek Kobza}, url = {http://ife.sk/wp-content/uploads/2020/09/foecol-2020-0008.pdf}, doi = {10.2478/foecol-2020-0017}, year = {2020}, date = {2020-11-24}, journal = {Folia Oecologica}, volume = {47}, number = {2}, pages = {144-152}, abstract = {Dothistroma needle blight (DNB) is a disease of pine needles. It causes significant defoliation of affected trees; intensive attacks lead to damages of the whole stand. The relationship of trends in disease severity and intensity with climatic variables were studied in three Austrian pine plantations (Jahodná, Kálnica, Litava) during 2014–2018. During the monitoring period, the greatest variability in disease severity was observed in the top third of the crowns, which showed the highest correlation with the variants of the most important climatic conditions (temperature and relative humidity) tested. For the spread of DNB, based on statistical assessment, a higher number of intervals of environmentally favourable climatic conditions is crucial. Both Dothistroma species (D. septosporum and D. pini), which are causal agents of the disease, were identified in Jahodná. In Kálnica and Litava, only D. septosporum was present.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a disease of pine needles. It causes significant defoliation of affected trees; intensive attacks lead to damages of the whole stand. The relationship of trends in disease severity and intensity with climatic variables were studied in three Austrian pine plantations (Jahodná, Kálnica, Litava) during 2014–2018. During the monitoring period, the greatest variability in disease severity was observed in the top third of the crowns, which showed the highest correlation with the variants of the most important climatic conditions (temperature and relative humidity) tested. For the spread of DNB, based on statistical assessment, a higher number of intervals of environmentally favourable climatic conditions is crucial. Both Dothistroma species (D. septosporum and D. pini), which are causal agents of the disease, were identified in Jahodná. In Kálnica and Litava, only D. septosporum was present. |
 | Drenkhan, R; Ganley, B; Martín-García, J; Vahalík, P; Adamson, K; Adamčíková, K; Ahumada, R; Blank, L; Bragança, H; Capretti, P; Cleary, M; Cornejo, C; Davydenko, K; Diez, J J; Lehtijärvi, Doğmuş H T; Dvořák, M; Enderle, R; Fourie, G; Georgieva, M; Ghelardini, L; Hantula, J; Ioos, R; Iturritxa, E; Kanetis, L; Karpun, N N; Koltay, A; Landeras, E; Markovskaja, S; Mesanza, N; Milenković, I; Musolin, D L; Nikolaou, K; Nowakowska, J A; Ogris, N; Oskay, F; Oszako, T; Papazova-Anakieva, I; Paraschiv, M; Pasquali, M; Pecori, F; Rafoss, T; Raitelaitytė, K; Raposo, R; Robin, C; Rodas, C A; A., Santini A; Sanz-Ros, A V; Selikhovkin, A V; Solla, A; Soukainen, M; Soulioti, N; Steenkamp, E T; Tsopelas, P; Vemić, A; Vettraino, A M; Wingfield, M J; Woodward, S; C., Zamora-Ballesteros C; Mullett, M S Global Geographic Distribution and Host Range of Fusarium circinatum, the Causal Agent of Pine Pitch Canker Journal Article Forests, 11 (7), 2020, ISSN: 1999-4907. @article{Drenkhan2020, title = {Global Geographic Distribution and Host Range of Fusarium circinatum, the Causal Agent of Pine Pitch Canker}, author = {R. Drenkhan and B. Ganley and J. Martín-García and P. Vahalík and K. Adamson and K. Adamčíková and R. Ahumada and L. Blank and H. Bragança and P. Capretti and M. Cleary and C. Cornejo and K. Davydenko and J.J. Diez and H.T. Doğmuş Lehtijärvi and M. Dvořák and R. Enderle and G. Fourie and M. Georgieva and L. Ghelardini and J. Hantula and R. Ioos and E. Iturritxa and L. Kanetis and N.N. Karpun and A. Koltay and E. Landeras and S. Markovskaja and N. Mesanza and I. Milenković and D.L. Musolin and K. Nikolaou and J.A. Nowakowska and N. Ogris and F. Oskay and T. Oszako and I. Papazova-Anakieva and M. Paraschiv and M. Pasquali and F. Pecori and T. Rafoss and K. Raitelaitytė and R. Raposo and C. Robin and C.A. Rodas and A. Santini A. and A.V. Sanz-Ros and A.V. Selikhovkin and A. Solla and M. Soukainen and N. Soulioti and E.T. Steenkamp and P. Tsopelas and A. Vemić and A.M. Vettraino and M.J. Wingfield and S. Woodward and C. Zamora-Ballesteros C. and M.S. Mullett }, doi = {doi:10.3390/f11070724}, issn = {1999-4907}, year = {2020}, date = {2020-07-01}, journal = {Forests}, volume = {11}, number = {7}, abstract = {Fusarium circinatum, the causal agent of pine pitch canker (PPC), is currently one of the most important threats of Pinus spp. globally. This pathogen is known in many pine-growing regions, including natural and planted forests, and can affect all life stages of trees, from emerging seedlings to mature trees. Despite the importance of PPC, the global distribution of F. circinatum is poorly documented, and this problem is also true of the hosts within countries that are affected. The aim of this study was to review the global distribution of F. circinatum, with a particular focus on Europe. We considered (1) the current and historical pathogen records, both positive and negative, based on confirmed reports from Europe and globally; (2) the genetic diversity and population structure of the pathogen; (3) the current distribution of PPC in Europe, comparing published models of predicted disease distribution; and (4) host susceptibility by reviewing literature and generating a comprehensive list of known hosts for the fungus. These data were collated from 41 countries and used to compile a specially constructed geo-database (http://bit.do/phytoportal). A review of 6297 observation records showed that F. circinatum and the symptoms it causes on conifers occurred in 14 countries, including four in Europe, and is absent in 28 countries. Field observations and experimental data from 138 host species revealed 106 susceptible host species including 85 Pinus species, 6 non-pine tree species and 15 grass and herb species. Our data confirm that susceptibility to F. circinatum varies between different host species, tree ages and environmental characteristics. Knowledge on the geographic distribution, host range and the relative susceptibility of different hosts is essential for disease management, mitigation and containment strategies. The findings reported in this review will support countries that are currently free of F. circinatum in implementing effective procedures and restrictions and prevent further spread of the pathogen.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fusarium circinatum, the causal agent of pine pitch canker (PPC), is currently one of the most important threats of Pinus spp. globally. This pathogen is known in many pine-growing regions, including natural and planted forests, and can affect all life stages of trees, from emerging seedlings to mature trees. Despite the importance of PPC, the global distribution of F. circinatum is poorly documented, and this problem is also true of the hosts within countries that are affected. The aim of this study was to review the global distribution of F. circinatum, with a particular focus on Europe. We considered (1) the current and historical pathogen records, both positive and negative, based on confirmed reports from Europe and globally; (2) the genetic diversity and population structure of the pathogen; (3) the current distribution of PPC in Europe, comparing published models of predicted disease distribution; and (4) host susceptibility by reviewing literature and generating a comprehensive list of known hosts for the fungus. These data were collated from 41 countries and used to compile a specially constructed geo-database (http://bit.do/phytoportal). A review of 6297 observation records showed that F. circinatum and the symptoms it causes on conifers occurred in 14 countries, including four in Europe, and is absent in 28 countries. Field observations and experimental data from 138 host species revealed 106 susceptible host species including 85 Pinus species, 6 non-pine tree species and 15 grass and herb species. Our data confirm that susceptibility to F. circinatum varies between different host species, tree ages and environmental characteristics. Knowledge on the geographic distribution, host range and the relative susceptibility of different hosts is essential for disease management, mitigation and containment strategies. The findings reported in this review will support countries that are currently free of F. circinatum in implementing effective procedures and restrictions and prevent further spread of the pathogen. |
 | Jančovičová, S; Adamčíková, K; Caboň, M; Adamčík, S How variable is Crepidotus variabilis? Journal Article Phytotaxa, 449 (3), pp. 243-264, 2020, ISSN: 1179-3155. @article{Jančovičová2020, title = {How variable is Crepidotus variabilis?}, author = {S. Jančovičová and K. Adamčíková and M. Caboň and S. Adamčík}, url = {http://ife.sk/wp-content/uploads/2016/10/fig-phytotaxa-sona-300x206.jpg}, doi = {doi.org/10.11646/phytotaxa.449.3.4}, issn = {1179-3155}, year = {2020}, date = {2020-06-23}, journal = {Phytotaxa}, volume = {449}, number = {3}, pages = {243-264}, abstract = {This study evaluates the morphological concept of Crepidotus variabilis and similar taxa based on basidiospore and cheilocystidia characters. A phylogenetic analysis of the ITS region clearly distinguished C. variabilis var. variabilis from the two other distinct taxa of the same lineage, C. variabilis var. trichocystis and C. neotrichocystis. We also recognised one additional morphologically similar and undescribed species that belongs to the C. variabilis lineage. All four taxa can also be recognised morphologically using basidiospore and cheilocystidia characters. Our detailed statistical comparison of these characters improves the knowledge of their variability and specifies more precisely the morphological circumscription of the taxa within this group.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study evaluates the morphological concept of Crepidotus variabilis and similar taxa based on basidiospore and cheilocystidia characters. A phylogenetic analysis of the ITS region clearly distinguished C. variabilis var. variabilis from the two other distinct taxa of the same lineage, C. variabilis var. trichocystis and C. neotrichocystis. We also recognised one additional morphologically similar and undescribed species that belongs to the C. variabilis lineage. All four taxa can also be recognised morphologically using basidiospore and cheilocystidia characters. Our detailed statistical comparison of these characters improves the knowledge of their variability and specifies more precisely the morphological circumscription of the taxa within this group. |
 | Pastirčáková, K; Adamčíková, K; Barta, M; Pažitný, J; Hoťka, P; Sarvašová, I; Kádasi-Horáková, M Host range of Hymenoscyphus fraxineus in Slovak arboreta Journal Article Forests, 11 (5), pp. 1-18, Article Number: 596, 2020, ISSN: 1999-4907. @article{Pastirčáková25.0, title = {Host range of \textit{Hymenoscyphus fraxineus} in Slovak arboreta}, author = {K. Pastirčáková and K. Adamčíková and M. Barta and J. Pažitný and P. Hoťka and I. Sarvašová and M. Kádasi-Horáková}, doi = {10.3390/f11050596}, issn = {1999-4907}, year = {2020}, date = {2020-05-25}, journal = {Forests}, volume = {11}, number = {5}, pages = {1-18, Article Number: 596}, abstract = {The health of 34 different Fraxinus taxa in association with the pathogenic fungus Hymenoscyphus fraxineus was assessed in four Slovak arboreta. Averaged across all arboreta, nearly one-quarter (24.9%) of all evaluated trees showed ash dieback symptoms. The damage was most serious on the common ash F. excelsior, a native species. The percentage of dead trees did not exceed 2% for all evaluated trees. Generally, ash trees of all ages were affected, though the intensity of the damage varied among the sites. The identity of H. fraxineus was confirmed by conventional PCR targeting the internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA, as well as the 18S gene/ITS-2 region of the rDNA operon. In Slovakia, the pathogen has expanded its host range from native species not only to their ornamental cultivars, but also to introduced North American (F. cinerea, F. latifolia, F. pennsylvanica, F. quadrangulata) and Asian (F. bungeana, F. chinensis ssp. rhynchophylla, F. mandshurica) ash species. H. fraxineus was also observed on the previous year’s leaf petioles of the native European species F. ornus, considered a weakly susceptible host. In Slovak arboreta, H. fraxineus was found on 23 Fraxinus taxa; 21 of them represent first records for the country. F. bungeana is recorded as a new host species of H. fraxineus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The health of 34 different Fraxinus taxa in association with the pathogenic fungus Hymenoscyphus fraxineus was assessed in four Slovak arboreta. Averaged across all arboreta, nearly one-quarter (24.9%) of all evaluated trees showed ash dieback symptoms. The damage was most serious on the common ash F. excelsior, a native species. The percentage of dead trees did not exceed 2% for all evaluated trees. Generally, ash trees of all ages were affected, though the intensity of the damage varied among the sites. The identity of H. fraxineus was confirmed by conventional PCR targeting the internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA, as well as the 18S gene/ITS-2 region of the rDNA operon. In Slovakia, the pathogen has expanded its host range from native species not only to their ornamental cultivars, but also to introduced North American (F. cinerea, F. latifolia, F. pennsylvanica, F. quadrangulata) and Asian (F. bungeana, F. chinensis ssp. rhynchophylla, F. mandshurica) ash species. H. fraxineus was also observed on the previous year’s leaf petioles of the native European species F. ornus, considered a weakly susceptible host. In Slovak arboreta, H. fraxineus was found on 23 Fraxinus taxa; 21 of them represent first records for the country. F. bungeana is recorded as a new host species of H. fraxineus. |
 | Buyck, B; Wang, X H; Adamčíková, K; Caboň, M; Jančovičová, S; Hofstetter, V; Adamčík, S One step closer to unravelling the origin of Russula: subgenus Glutinosae subg. nov. Journal Article Mycosphere, 11 (1), pp. 285–304, 2020, ISSN: 2077 7019. @article{Buyck2020, title = {One step closer to unravelling the origin of Russula: subgenus Glutinosae subg. nov.}, author = {B. Buyck and X. H. Wang and K. Adamčíková and M. Caboň and S Jančovičová and V. Hofstetter and S. Adamčík }, doi = {Doi 10.5943/mycosphere/11/1/6}, issn = {2077 7019}, year = {2020}, date = {2020-01-31}, journal = {Mycosphere}, volume = {11}, number = {1}, pages = {285–304}, abstract = {This study reports on the discovery of a new subgenus, Russula subg. Glutinosae, having an Eastern North American – East Asian distribution. A multigene phylogeny places this new subgenus sister with strong support to a well-supported clade composed of subgenera Compactae and Archaeae. It holds only two very rare, northern hemisphere species, the North American R. glutinosa and the Asian R. glutinosoides sp. nov., thereby adding support to a northern hemisphere origin of the genus. Russula fattoensis is here shown to be a synonym of R. glutinosa. Detailed morphological descriptions and illustrations of holotype collections are provided and potential affinities and similarities with other subgenera are discussed. The new subgenus is a perfect illustration of the fact that nBLAST of nrITS does not always provide the appropriate sampling for phylogenetic analyses.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study reports on the discovery of a new subgenus, Russula subg. Glutinosae, having an Eastern North American – East Asian distribution. A multigene phylogeny places this new subgenus sister with strong support to a well-supported clade composed of subgenera Compactae and Archaeae. It holds only two very rare, northern hemisphere species, the North American R. glutinosa and the Asian R. glutinosoides sp. nov., thereby adding support to a northern hemisphere origin of the genus. Russula fattoensis is here shown to be a synonym of R. glutinosa. Detailed morphological descriptions and illustrations of holotype collections are provided and potential affinities and similarities with other subgenera are discussed. The new subgenus is a perfect illustration of the fact that nBLAST of nrITS does not always provide the appropriate sampling for phylogenetic analyses. |
 | Adamčík, S; Dima, B; Adamčíková, K; Corriol, G; Læssøe, T; Moreau, P -A; Caboň, M; Jančovičová, S Hodophilus phaeophyllus complex (Clavariaceae, Agaricales) is defined as new phylogenetic lineage in Europe Journal Article Mycological Progress, 19 (2), pp. 111-125, 2020, ISSN: 1617-416X. @article{Adamčík2020, title = {Hodophilus phaeophyllus complex (Clavariaceae, Agaricales) is defined as new phylogenetic lineage in Europe}, author = {S. Adamčík and B. Dima and K. Adamčíková and G. Corriol and T. Læssøe and P.-A. Moreau and M. Caboň and S. Jančovičová }, doi = {DOI 10.1007/s11557-019-01544-9}, issn = {1617-416X}, year = {2020}, date = {2020-01-30}, journal = {Mycological Progress}, volume = {19}, number = {2}, pages = {111-125}, abstract = {The study deals with the last unexplored morphological group of the genus Hodophilus defined by absence of distinct odours, absence of yellow colours and absence of darker dots on the stipe. The phylogenetic reconstruction of the whole genus based on nrITS, nrLSU and RPB2 sequences placed all European members having these morphological characters in a monophyletic group defined here as a new section H. sect. Phaeophylli. The remaining European members of the genus are placed in two additional groups classified as section H. sect. Hodophilus and the new section H. sect. Micacei. Five species are recognised within section Phaeophylli which is typified by H. phaeophyllus that is lecto- and epitypified. Three new species belonging to this section are described: H. carpathicus, H. decurrentior and H. stramineus. Hodophilus decurrentior is the only species showing distinct morphological differences under the microscope. The identification of other species of the section depends mainly on the colour of basidiomata. An updated key to all European members of the genus is provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The study deals with the last unexplored morphological group of the genus Hodophilus defined by absence of distinct odours, absence of yellow colours and absence of darker dots on the stipe. The phylogenetic reconstruction of the whole genus based on nrITS, nrLSU and RPB2 sequences placed all European members having these morphological characters in a monophyletic group defined here as a new section H. sect. Phaeophylli. The remaining European members of the genus are placed in two additional groups classified as section H. sect. Hodophilus and the new section H. sect. Micacei. Five species are recognised within section Phaeophylli which is typified by H. phaeophyllus that is lecto- and epitypified. Three new species belonging to this section are described: H. carpathicus, H. decurrentior and H. stramineus. Hodophilus decurrentior is the only species showing distinct morphological differences under the microscope. The identification of other species of the section depends mainly on the colour of basidiomata. An updated key to all European members of the genus is provided. |
 | Elvira-Recuenco, M; Cacciola, S O; Sanz-Ros, A V; Garbelotto, M; Aguayo, J; Solla, A; Mullett, M; Drenkhan, T; Oskay, F; Kaya, A G A; Iturritxa, E; Cleary, M; Witzell, J; Georgieva, M; Papazova-Anakieva, I; Chira, D; Paraschiv, M; Musolin, D L; Selikhovkin, A V; Varentsova, Yu. E; Adamčíková, K; Markovskaja, S; Mesanza, N; Davydenko, K; Capretti, P; Scanu, B; Gonthier, P; Tsopelas, P; Martín-García, J; Morales-Rodríguez, C; Lehtijärvi, A; Lehtijärvi, Doğmuş H T; Oszako, T; Nowakowska, J A; Bragança, H; Fernández-Fernández, M; Hantula, J; Díez, J J Potential Interactions Between Invasive Fusarium circinatum and Other Pine Pathogens in Europe Journal Article Forests, 11 (1), 2020, ISSN: 1999-4907. @article{Elvira-Recuenco2019, title = {Potential Interactions Between Invasive Fusarium circinatum and Other Pine Pathogens in Europe}, author = {M. Elvira-Recuenco and S. O. Cacciola and A. V. Sanz-Ros and M. Garbelotto and J. Aguayo and A. Solla and M. Mullett and T. Drenkhan and F. Oskay and A. G. A. Kaya and E. Iturritxa and M. Cleary and J. Witzell and M. Georgieva and I. Papazova-Anakieva and D. Chira and M. Paraschiv and D.L. Musolin and A.V. Selikhovkin and E. Yu. Varentsova and K. Adamčíková and S. Markovskaja and N. Mesanza and K. Davydenko and P. Capretti and B. Scanu and P. Gonthier and P. Tsopelas and J. Martín-García and C. Morales-Rodríguez and A. Lehtijärvi and H. T. Doğmuş Lehtijärvi and T. Oszako and J.A. Nowakowska and H. Bragança and M. Fernández-Fernández and J. Hantula and J.J. Díez }, doi = {doi:10.3390/f11010007}, issn = {1999-4907}, year = {2020}, date = {2020-01-18}, journal = {Forests}, volume = {11}, number = {1}, abstract = {Pines are major components of native forests and plantations in Europe, where they have both economic significance and an important ecological role. Diseases of pines are mainly caused by fungal and oomycete pathogens, and can significantly reduce the survival, vigor, and yield of both individual trees and entire stands or plantations. Pine pitch canker (PPC), caused by Fusarium circinatum (Nirenberg and O’Donnell), is among the most devastating pine diseases in the world, and is an example of an emergent invasive disease in Europe. The effects of microbial interactions on plant health, as well as the possible roles plant microbiomes may have in disease expression, have been the focus of several recent studies. Here, we describe the possible effects of co-infection with pathogenic fungi and oomycetes with F. circinatum on the health of pine seedlings and mature plants, in an attempt to expand our understanding of the role that biotic interactions may play in the future of PPC disease in European nurseries and forests. The available information on pine pathogens that are able to co-occur with F. circinatum in Europe is here reviewed and interpreted to theoretically predict the effects of such co-occurrences on pine survival, growth, and yield. Beside the awareness that F. circinatum may co-occurr on pines with other pathogens, an additional outcome from this review is an updating of the literature, including the so-called grey literature, to document the geographical distribution of the relevant pathogens and to facilitate differential diagnoses, particularly in nurseries, where some of them may cause symptoms similar to those induced by F. circinatum. An early and accurate diagnosis of F. circinatum, a pathogen that has been recently introduced and that is currently regulated in Europe, is essential to prevent its introduction and spread in plantings and forests.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Pines are major components of native forests and plantations in Europe, where they have both economic significance and an important ecological role. Diseases of pines are mainly caused by fungal and oomycete pathogens, and can significantly reduce the survival, vigor, and yield of both individual trees and entire stands or plantations. Pine pitch canker (PPC), caused by Fusarium circinatum (Nirenberg and O’Donnell), is among the most devastating pine diseases in the world, and is an example of an emergent invasive disease in Europe. The effects of microbial interactions on plant health, as well as the possible roles plant microbiomes may have in disease expression, have been the focus of several recent studies. Here, we describe the possible effects of co-infection with pathogenic fungi and oomycetes with F. circinatum on the health of pine seedlings and mature plants, in an attempt to expand our understanding of the role that biotic interactions may play in the future of PPC disease in European nurseries and forests. The available information on pine pathogens that are able to co-occur with F. circinatum in Europe is here reviewed and interpreted to theoretically predict the effects of such co-occurrences on pine survival, growth, and yield. Beside the awareness that F. circinatum may co-occurr on pines with other pathogens, an additional outcome from this review is an updating of the literature, including the so-called grey literature, to document the geographical distribution of the relevant pathogens and to facilitate differential diagnoses, particularly in nurseries, where some of them may cause symptoms similar to those induced by F. circinatum. An early and accurate diagnosis of F. circinatum, a pathogen that has been recently introduced and that is currently regulated in Europe, is essential to prevent its introduction and spread in plantings and forests. |
2019 |
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Adamčík, S; Looney, B; Caboň, M; Jančovičová, S; Adamčíková, K; Avis, P G; Barajas, M; Bhatt, R P; Corrales, A; Das, K; Hampe, F; Ghosh, A; Gates, G; Kälviäinen, V; Khalid, A N; Kiran, M; Lange, De R; Lee, H; Lim, Y W; Kong, A; Manz, C; Ovrebo, C; Saba, M; Taipale, T; Verbeken, A; Wisitrassameewong, K; Buyck, B The quest for a globally comprehensible Russula language Journal Article Fungal Diversity, 99 (1), pp. 369-449, 2019, ISSN: 1560-2745. @article{Adamčík2019, title = {The quest for a globally comprehensible Russula language}, author = {S. Adamčík and B. Looney and M. Caboň and S. Jančovičová and K. Adamčíková and P. G. Avis and M. Barajas and R. P. Bhatt and A. Corrales and K. Das and F. Hampe and A. Ghosh and G. Gates and V. Kälviäinen and A. N. Khalid and M. Kiran and R. De Lange and H. Lee and Y. W. Lim and A. Kong and C. Manz and C. Ovrebo and M. Saba and T. Taipale and A. Verbeken and K. Wisitrassameewong and B. Buyck}, doi = {doi.org/10.1007/s13225-019-00437-2}, issn = {1560-2745}, year = {2019}, date = {2019-10-24}, journal = {Fungal Diversity}, volume = {99}, number = {1}, pages = {369-449}, abstract = {Since 2007, the quality of Russula descriptions has improved and the use of molecular support for species delimitation and the number of published new species has increased. However, the description style is not consistent and has regional or author-specific patterns. Most recent publications still favour descriptions of spores compared to hymenium and pileipellis elements, and usually only the spore size is provided with statistical support. This study proposes standards for descriptions of the microscopic structure of Russula species (Russulaceae, Agaricomycetes). We present the description template, the template measurements table, the specific terminology and the essential chemical reagents. The proposed standards were tested by mycologists from 11 countries who met at the Russula Microscopy Workshop in Slovakia. Descriptions of 26 species from 9 countries and four continents were prepared, among them R. amarissima, R. castanopsidis, R. seperina and R. subtilis are re-described and 15 species are introduced as new: R. abietiphila, R. amerorecondita, R. aurantioflava, R. echidna, R. flavobrunnescens, R. fluvialis, R. fortunae, R. garyensis, R. gemmata, R. laevis, R. madrensis, R. olivaceohimalayensis, R. purpureogracilis, R. sancti-pauli and R. wielangtae. Seven descriptions for candidate new species are provided without a formal name assignment. Pairwise comparison of species described in this study with available similar descriptions of related species suggests that microscopic characters from all parts of the basidiomata can be equally important for species recognition and they deserve the same treatment including number of measurements and statistics. The majority of recent studies does not recognise differences between the pileus margin and centre, but more than one-third of the species described in this study show distinct differences between the pileus areas, emphasizing the importance to specify the origin of pileipellis observations. This study proved that there is frequently insufficient difference in the ITS barcode between closely related species and that it is necessary to use more genetic markers combined with ecological and geographical data.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Since 2007, the quality of Russula descriptions has improved and the use of molecular support for species delimitation and the number of published new species has increased. However, the description style is not consistent and has regional or author-specific patterns. Most recent publications still favour descriptions of spores compared to hymenium and pileipellis elements, and usually only the spore size is provided with statistical support. This study proposes standards for descriptions of the microscopic structure of Russula species (Russulaceae, Agaricomycetes). We present the description template, the template measurements table, the specific terminology and the essential chemical reagents. The proposed standards were tested by mycologists from 11 countries who met at the Russula Microscopy Workshop in Slovakia. Descriptions of 26 species from 9 countries and four continents were prepared, among them R. amarissima, R. castanopsidis, R. seperina and R. subtilis are re-described and 15 species are introduced as new: R. abietiphila, R. amerorecondita, R. aurantioflava, R. echidna, R. flavobrunnescens, R. fluvialis, R. fortunae, R. garyensis, R. gemmata, R. laevis, R. madrensis, R. olivaceohimalayensis, R. purpureogracilis, R. sancti-pauli and R. wielangtae. Seven descriptions for candidate new species are provided without a formal name assignment. Pairwise comparison of species described in this study with available similar descriptions of related species suggests that microscopic characters from all parts of the basidiomata can be equally important for species recognition and they deserve the same treatment including number of measurements and statistics. The majority of recent studies does not recognise differences between the pileus margin and centre, but more than one-third of the species described in this study show distinct differences between the pileus areas, emphasizing the importance to specify the origin of pileipellis observations. This study proved that there is frequently insufficient difference in the ITS barcode between closely related species and that it is necessary to use more genetic markers combined with ecological and geographical data. | |
 | K. Adamčíková E. Ondrušková, Kobza M Hypovirulence in chestnut blight fungus, Cryphonectria parasitica, in Slovakia Journal Article Biocontrol Science and Technology, 29 (9), pp. 840-851, 2019, ISSN: 0958-3157. @article{Adamčíková2019, title = {Hypovirulence in chestnut blight fungus, Cryphonectria parasitica, in Slovakia}, author = {K. Adamčíková, E. Ondrušková, M. Kobza}, doi = {DOI: 10.1080/09583157.2019.1608509}, issn = {0958-3157}, year = {2019}, date = {2019-07-19}, journal = {Biocontrol Science and Technology}, volume = {29}, number = {9}, pages = {840-851}, abstract = {The recovery of the European chestnut from chestnut blight in Europe is attributed to hypovirulence, a viral disease that reduces the virulence of its host – Cryphonectria parasitica. Hypovirulence has been used in biocontrol programmes throughout Europe with the aim of not only treating individual chestnut blight cankers but causing the natural spread and establishment of viruses in C. parasitica populations. Almost 40 years after the first appearance of chestnut blight and more than 20 years after the first biocontrol treatment implementation in Slovakia the establishment and natural spread of hypovirulence were studied. The morphological characteristics of 179 chestnut blight cankers and 161 C. parasitica strains were evaluated for the presence of hypovirus from three chestnut cultivation sites in Slovakia. Nineteen cankers and isolates were considered hypovirulent based on visual assessment of their morphological characteristics. The hypovirus presence by RNA extraction was confirmed in 6 cankers/strains.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The recovery of the European chestnut from chestnut blight in Europe is attributed to hypovirulence, a viral disease that reduces the virulence of its host – Cryphonectria parasitica. Hypovirulence has been used in biocontrol programmes throughout Europe with the aim of not only treating individual chestnut blight cankers but causing the natural spread and establishment of viruses in C. parasitica populations. Almost 40 years after the first appearance of chestnut blight and more than 20 years after the first biocontrol treatment implementation in Slovakia the establishment and natural spread of hypovirulence were studied. The morphological characteristics of 179 chestnut blight cankers and 161 C. parasitica strains were evaluated for the presence of hypovirus from three chestnut cultivation sites in Slovakia. Nineteen cankers and isolates were considered hypovirulent based on visual assessment of their morphological characteristics. The hypovirus presence by RNA extraction was confirmed in 6 cankers/strains. |
 | Pažitný, J; Kobza, M; Adamčíková, K Variance in leaf spot susceptibility in chestnut trees of different species and hybrids Journal Article Journal of Plant Disease and Protection, 126 (2), pp. 169-172, 2019, ISSN: 1861-3829. @article{Pažitný2019, title = {Variance in leaf spot susceptibility in chestnut trees of different species and hybrids}, author = {J. Pažitný and M. Kobza and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s41348-019-00209-9}, doi = {DOI 10.1007/s41348-019-00209-9}, issn = {1861-3829}, year = {2019}, date = {2019-02-19}, journal = {Journal of Plant Disease and Protection}, volume = {126}, number = {2}, pages = {169-172}, abstract = {Prior to the first occurrence of chestnut blight in 1976, leaf spot caused by Cylindrosporium castaneae was one of the most important diseases of European chestnut trees in Slovakia. In 2011, extensive damage of leaves by leaf spot was recorded in young chestnut trees in an experimental chestnut orchard in Pribelce, Slovakia, where seedlings and grafts of different taxonomic origin (Castanea sativa, Castanea crenata, and C. sativa × C. crenata) exhibited visible differences in the extent of leaf spot. Digital images of particular leaves were evaluated using Quick Micro Photo software and the ratio of infected leaf area was calculated. Morphological observations of the fungal species causing leaf spot were conducted and the disease symptoms were described and discussed. Taxonomic origin of studied trees showed a significant effect on the proportion of leaf spot area, indicating that the response to fungal attacks has a strong genetic determinism, with resistance being clearly influenced by taxonomic origin. The lowest leaf spot infection was recorded in C. crenata seedlings. Significant differences were noted between the individual trees tested and between the studied years.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Prior to the first occurrence of chestnut blight in 1976, leaf spot caused by Cylindrosporium castaneae was one of the most important diseases of European chestnut trees in Slovakia. In 2011, extensive damage of leaves by leaf spot was recorded in young chestnut trees in an experimental chestnut orchard in Pribelce, Slovakia, where seedlings and grafts of different taxonomic origin (Castanea sativa, Castanea crenata, and C. sativa × C. crenata) exhibited visible differences in the extent of leaf spot. Digital images of particular leaves were evaluated using Quick Micro Photo software and the ratio of infected leaf area was calculated. Morphological observations of the fungal species causing leaf spot were conducted and the disease symptoms were described and discussed. Taxonomic origin of studied trees showed a significant effect on the proportion of leaf spot area, indicating that the response to fungal attacks has a strong genetic determinism, with resistance being clearly influenced by taxonomic origin. The lowest leaf spot infection was recorded in C. crenata seedlings. Significant differences were noted between the individual trees tested and between the studied years. |
2018 |
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 | Bolvanský, M; Pažitný, J; Adamčíková, K Grading of blight resistance in different chestnut accessions. Journal Article Acta Horticulturae, 1220 , pp. 87-94, 2018, ISSN: 05677572. @article{M.2018, title = {Grading of blight resistance in different chestnut accessions.}, author = {M. Bolvanský and J. Pažitný and K. Adamčíková}, url = {https://www.actahort.org/members/showpdf?booknrarnr=1220_13}, doi = {10.17660/ActaHortic.2018.1220.13}, issn = {05677572}, year = {2018}, date = {2018-11-01}, journal = {Acta Horticulturae}, volume = {1220}, pages = {87-94}, abstract = {Five chestnut trees (three hybrids Castanea sativa × C. crenata, one C. sativa and one C. crenata) with different levels of blight resistance were subjected to the screening by two virulent and one hypovirulent strains of Cryphonectria parasitica in order to examine reliability of ex situ tests for grading of blight resistance in chestnut. Branches 2-3 cm in diameter were harvested from the trees, in early April and end of September and stored at about 10°C for either 2 weeks (for branch sections) or 4 weeks (for bark- and wood-tissue sections). Branch sections and bark- and wood-tissue sections were inoculated with the C. parasitica strains and incubated in the dark at 25°C with 95% humidity for 4 weeks and/or 4 days, respectively. In branch sections, superficial cankers were very rare so only occurrence of pycnidia formed around the inoculating holes were evaluated. Pycnidia occurred more frequently in branch sections collected from the less resistant accessions in October trial. The size of necrotic areas reflected the resistance ranking of accessions in April/May better than in October inoculation trials. Results of inoculations on excised wood tissue were more consistent with the real blight resistance level than data obtained from inoculations on excised bark tissue. Occasional inconsistence between results of inoculations carried out at different dates or in different trees might be caused by different physiological state of collected branches. Based on these results it can be concluded that the end of dormant period seems to be the most suitable time to carry out both pathogenicity and resistance tests in chestnuts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five chestnut trees (three hybrids Castanea sativa × C. crenata, one C. sativa and one C. crenata) with different levels of blight resistance were subjected to the screening by two virulent and one hypovirulent strains of Cryphonectria parasitica in order to examine reliability of ex situ tests for grading of blight resistance in chestnut. Branches 2-3 cm in diameter were harvested from the trees, in early April and end of September and stored at about 10°C for either 2 weeks (for branch sections) or 4 weeks (for bark- and wood-tissue sections). Branch sections and bark- and wood-tissue sections were inoculated with the C. parasitica strains and incubated in the dark at 25°C with 95% humidity for 4 weeks and/or 4 days, respectively. In branch sections, superficial cankers were very rare so only occurrence of pycnidia formed around the inoculating holes were evaluated. Pycnidia occurred more frequently in branch sections collected from the less resistant accessions in October trial. The size of necrotic areas reflected the resistance ranking of accessions in April/May better than in October inoculation trials. Results of inoculations on excised wood tissue were more consistent with the real blight resistance level than data obtained from inoculations on excised bark tissue. Occasional inconsistence between results of inoculations carried out at different dates or in different trees might be caused by different physiological state of collected branches. Based on these results it can be concluded that the end of dormant period seems to be the most suitable time to carry out both pathogenicity and resistance tests in chestnuts. |
 | Adamčíková, K; Pažitný, J; Pastirčáková, K Individual resistance of Fraxinus angustifolia and F. excelsior clones to Hymenoscyphus fraxineus Journal Article Journal of Plant Protection Research, 58 (3), pp. 227-233, 2018, ISSN: 1427-4345. @article{Adamčíková2018, title = {Individual resistance of \textit{Fraxinus angustifolia} and \textit{F. excelsior} clones to \textit{Hymenoscyphus fraxineus}}, author = {K. Adamčíková and J. Pažitný and K. Pastirčáková}, doi = {10.24425/122937}, issn = {1427-4345}, year = {2018}, date = {2018-10-22}, journal = {Journal of Plant Protection Research}, volume = {58}, number = {3}, pages = {227-233}, abstract = {Ash dieback, caused by Hymenoscyphus fraxineus, is a serious disease of common and narrow-leaved ash in Europe. The resistance of individual trees seems to be important for the maintenance of ash in European forests. In this in situ wound inoculation study, the susceptibility and differences in resistance to H. fraxineus between Fraxinus excelsior and F. angustifolia clones were assessed. Neither of the tested clones revealed total resistance to ash dieback; variety between the tested clones was observed. Differences in necroses lengths were significant between clones and between two ash species. Longer necroses were formed in F. angustifolia than in F. excelsior. Some clones exhibiting some resistance to the pathogen were identified. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Ash dieback, caused by Hymenoscyphus fraxineus, is a serious disease of common and narrow-leaved ash in Europe. The resistance of individual trees seems to be important for the maintenance of ash in European forests. In this in situ wound inoculation study, the susceptibility and differences in resistance to H. fraxineus between Fraxinus excelsior and F. angustifolia clones were assessed. Neither of the tested clones revealed total resistance to ash dieback; variety between the tested clones was observed. Differences in necroses lengths were significant between clones and between two ash species. Longer necroses were formed in F. angustifolia than in F. excelsior. Some clones exhibiting some resistance to the pathogen were identified. |
 | Pastirčáková, K; Adamčíková, K; Pastirčák, M; Zach, P; Galko, J; Kováč, M; Laco, J Two blue-stain fungi colonizing Scots pine (Pinus sylvestris) trees infested by bark beetles in Slovakia, Central Europe Journal Article Biologia, 73 (11), pp. 1053-1066, 2018, ISSN: 0006-3088. @article{Pastirčáková2018c, title = {Two blue-stain fungi colonizing Scots pine (\textit{Pinus sylvestris}) trees infested by bark beetles in Slovakia, Central Europe}, author = {K. Pastirčáková and K. Adamčíková and M. Pastirčák and P. Zach and J. Galko and M. Kováč and J. Laco}, doi = {10.2478/s11756-018-0114-6}, issn = {0006-3088}, year = {2018}, date = {2018-09-17}, journal = {Biologia}, volume = {73}, number = {11}, pages = {1053-1066}, abstract = {Excessive mortality of Scots pine (Pinus sylvestris) trees has recently been observed in the Záhorská nížina lowland in western Slovakia, Central Europe. Continuous drought, bark beetles and blue-stain fungi are likely to play a crucial role in this dieback of pines in a warm sandy area. Two blue-stain fungi, Ophiostoma ips and Ophiostoma minus, were found here on Scots pine trunks infested by bark beetles. These fungi were isolated and identified based on morphological properties and DNA sequences (ITS, LSU, β-tubulin). They colonized the inner bark, phloem and blue-stained sapwood, and were recorded in the galleries of Orthotomicus longicollis and other species of this genus. The occurrence of O. minus was massive; the fungus was found to produce perithecia abundantly in dying pines. Detailed descriptions, illustrations and global distribution of the two fungal species on host pine species are provided. The records of O. ips and those of O. minus are new for the mycoflora of Slovakia. Undetermined mycophagous nematodes were found inside the perithecia of O. ips. The role of bark beetles as real and potential vectors (Ips spp., Orthotomicus spp., Tomicus spp., Hylurgus ligniperda, Hylastes sp.) of blue-stain fungi in a declining pine forest is discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Excessive mortality of Scots pine (Pinus sylvestris) trees has recently been observed in the Záhorská nížina lowland in western Slovakia, Central Europe. Continuous drought, bark beetles and blue-stain fungi are likely to play a crucial role in this dieback of pines in a warm sandy area. Two blue-stain fungi, Ophiostoma ips and Ophiostoma minus, were found here on Scots pine trunks infested by bark beetles. These fungi were isolated and identified based on morphological properties and DNA sequences (ITS, LSU, β-tubulin). They colonized the inner bark, phloem and blue-stained sapwood, and were recorded in the galleries of Orthotomicus longicollis and other species of this genus. The occurrence of O. minus was massive; the fungus was found to produce perithecia abundantly in dying pines. Detailed descriptions, illustrations and global distribution of the two fungal species on host pine species are provided. The records of O. ips and those of O. minus are new for the mycoflora of Slovakia. Undetermined mycophagous nematodes were found inside the perithecia of O. ips. The role of bark beetles as real and potential vectors (Ips spp., Orthotomicus spp., Tomicus spp., Hylurgus ligniperda, Hylastes sp.) of blue-stain fungi in a declining pine forest is discussed. |
 | Pažitný, J; Bolvanský, M; Adamčíková, K Screening for resistance of progenies derived from Castanea sativa × C. crenata and C. crenata to Cryphonectria parasitica Journal Article Forest Pathology, 48 (5), pp. e12439, 2018, ISSN: 1439-0329. @article{Pažitný2018, title = {Screening for resistance of progenies derived from Castanea sativa × C. crenata and C. crenata to Cryphonectria parasitica}, author = {J. Pažitný and M. Bolvanský and K. Adamčíková}, url = {https://onlinelibrary.wiley.com/doi/10.1111/efp.12439}, doi = {10.1111/efp.12439}, issn = {1439-0329}, year = {2018}, date = {2018-08-10}, journal = {Forest Pathology}, volume = {48}, number = {5}, pages = {e12439}, abstract = {The possibility of screening young seedlings of Castanea for chestnut blight resistance was examined to try to shorten time needed for a breeding programme. Two-year-old chestnut seedlings of eight progenies, derived from open and controlled pollination of three hybrids (Castanea sativa × C. crenata and a C. crenata tree by paternal tree of C. sativa) were screened for resistance to chestnut blight. One hybrid and a C. crenata were partially susceptible, and two hybrids were partially resistant to chestnut blight. A total of 179 seedlings were inoculated with two virulent and one hypovirulent strain of Cryphonectria parasitica at two dates (early June and early September). Stromata production was higher and canker size larger when seedlings were inoculated in early June. Differences in canker size among seedlings derived from different parental trees were observed only after September inoculation. However, differences in canker size among seedlings inoculated with the virulent strains were observed only after June inoculation. The only significant effect on seedling survival was based on fungal strains used for inoculation. Ratio of seedlings, which died after inoculation with the hypovirulent strain, was significantly lower (36.8%) than those that died after inoculation with virulent strains (88.4 and 95.1%). Survival of seedlings was affected only by fungal strains used in inoculation, and not influenced by parental trees. The risk of seedling dieback increased with increasing canker size and with decreasing stem diameter of seedlings. }, keywords = {}, pubstate = {published}, tppubtype = {article} } The possibility of screening young seedlings of Castanea for chestnut blight resistance was examined to try to shorten time needed for a breeding programme. Two-year-old chestnut seedlings of eight progenies, derived from open and controlled pollination of three hybrids (Castanea sativa × C. crenata and a C. crenata tree by paternal tree of C. sativa) were screened for resistance to chestnut blight. One hybrid and a C. crenata were partially susceptible, and two hybrids were partially resistant to chestnut blight. A total of 179 seedlings were inoculated with two virulent and one hypovirulent strain of Cryphonectria parasitica at two dates (early June and early September). Stromata production was higher and canker size larger when seedlings were inoculated in early June. Differences in canker size among seedlings derived from different parental trees were observed only after September inoculation. However, differences in canker size among seedlings inoculated with the virulent strains were observed only after June inoculation. The only significant effect on seedling survival was based on fungal strains used for inoculation. Ratio of seedlings, which died after inoculation with the hypovirulent strain, was significantly lower (36.8%) than those that died after inoculation with virulent strains (88.4 and 95.1%). Survival of seedlings was affected only by fungal strains used in inoculation, and not influenced by parental trees. The risk of seedling dieback increased with increasing canker size and with decreasing stem diameter of seedlings. |
 | Crous, P W; Wingfield, M J; Burgess, T I; Hardy, G.E.St.J.; Gené, J; Guarro, J; García, Baseia I G D; Gusmão, L F P; Souza-Motta, C M; Thangavel, R; Adamčík, S; Barili, A; Barnes, C W; Bezerra, J D P; Bordallo, J J; Cano-Lira, J F; de Oliveira, R J V; Ercole, E; Hubka, V; Iturrieta-González, I; Kubátová, A; Martín, M P; Moreau, P -A; Morte, A; Ordoñez, M E; Rodríguez, A; Stchigel, A M; Vizzini, A; Abdollahzadeh, J; Abreu, V P; Adamčíková, K; Albuquerque, G M R; Alexandrova, A V; Álvarez_Duarte, E; Armstrong-Cho, C; Banniza, S; Barbosa, R N; Bellanger, J -M; Bezerra, J L; Cabral, T S; Caicedo, Caboňand M E; Cantillo, T; Carnegie, A J; Carmo, L T; Castañeda-Ruiz, R F; Clement, C R; Čmoková, A; Conceição, L B; Cruz, R H S F; Damm, U; da_Silva, B D B; da_Silva, G A; da_Silva, R M F; Santiago, A.L.C.M._de_A.; de_Oliveira, L F; de_Souza, C A F; Déniel, F; Dima, B; Dong, G; Edwards, J; Félix, C R; Fournier, J; Gibertoni, T B; Hosaka, K; Iturriaga, T; Jadan, M; Jany, J -L; Jurjević, Ž; Kolařík, M; Kušan, I; Landell, M F; Leite_Cordeiro, T R; Lima, X D; Loizides, M; Luo, S; Machado, A R; Madrid, H; Magalhães, O M C; Marinho, P; Matočec, N; Mešić, A; Miller, A N; Morozova, O V; Neves, R P; Nonaka, K; Nováková, A; Oberlies, N H; Oliveira-Filho, J R C; Oliveira, T G L; Papp, V; Pereira, O L; Perrone, G; Peterson, S W; Pham, T H G; Raja, H A; Raudabaugh, D B; Řehulka, J; RodrÃguez-Andrade, E; Saba, M; Schauflerova, A; Shivas, R G; Simonini, G; Siqueira, J P Z; Sousa, J O; Stajsic, V; Svetasheva, T; Tan, Y P; Tkalčec, Z; Ullah, S; Valente, P; Valenzuela-Lopez, N; Abrinbana, M; Viana_Marques, D A; Wong, P T W; Xavier_de_Lima, V; Groenewald, J Z Fungal Planet description sheets: 716-784 Journal Article Persoonia, 40 , pp. 240-393, 2018, ISSN: 1878-9080. @article{Crous2018, title = {Fungal Planet description sheets: 716-784}, author = {P.W. Crous and M.J. Wingfield and T.I. Burgess and G.E.St.J. Hardy and J. Gené and J. Guarro and I.G. Baseia D. García and L.F.P. Gusmão and C.M. Souza-Motta and R. Thangavel and S. Adamčík and A. Barili and C.W. Barnes and J.D.P. Bezerra and J.J. Bordallo and J.F. Cano-Lira and R.J.V. de Oliveira and E. Ercole and V. Hubka and I. Iturrieta-González and A. Kubátová and M.P. Martín and P.-A. Moreau and A. Morte and M.E. Ordoñez and A. Rodríguez and A.M. Stchigel and A. Vizzini and J. Abdollahzadeh and V.P. Abreu and K. Adamčíková and G.M.R. Albuquerque and A.V. Alexandrova and E. Álvarez_Duarte and C. Armstrong-Cho and S. Banniza and R.N. Barbosa and J.-M. Bellanger and J.L. Bezerra and T.S. Cabral and M. Caboňand E. Caicedo and T. Cantillo and A.J. Carnegie and L.T. Carmo and R.F. Castañeda-Ruiz and C.R. Clement and A. Čmoková and L.B. Conceição and R.H.S.F Cruz and U. Damm and B.D.B. da_Silva and G.A. da_Silva and R.M.F. da_Silva and A.L.C.M._de_A. Santiago and L.F. de_Oliveira and C.A.F. de_Souza and F. Déniel and B. Dima and G. Dong and J. Edwards and C.R. Félix and J. Fournier and T.B. Gibertoni and K. Hosaka and T. Iturriaga and M. Jadan and J.-L. Jany and Ž. Jurjević and M. Kolařík and I. Kušan and M.F. Landell and T.R. Leite_Cordeiro and X.D. Lima and M. Loizides and S. Luo and A.R. Machado and H. Madrid and O.M.C. Magalhães and P. Marinho and N. Matočec and A. Mešić and A.N. Miller and O.V. Morozova and R.P. Neves and K. Nonaka and A. Nováková and N.H. Oberlies and J.R.C. Oliveira-Filho and T.G.L. Oliveira and V. Papp and O.L. Pereira and G. Perrone and S.W. Peterson and T.H.G. Pham and H.A. Raja and D.B. Raudabaugh and J. Řehulka and E. RodrÃguez-Andrade and M. Saba and A. Schauflerova and R.G. Shivas and G. Simonini and J.P.Z. Siqueira and J.O. Sousa and V. Stajsic and T. Svetasheva and Y.P. Tan and Z. Tkalčec and S. Ullah and P. Valente and N. Valenzuela-Lopez and M. Abrinbana and D.A. Viana_Marques and P.T.W. Wong and V. Xavier_de_Lima and J.Z. Groenewald}, doi = {https://doi.org/10.3767/persoonia.2018.40.10}, issn = {1878-9080}, year = {2018}, date = {2018-07-13}, journal = {Persoonia}, volume = {40}, pages = {240-393}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
 | Adamčík, S; Dima, B; Adamčíková, K; Læssøe, T; Moreau, P A; Vizzini, A; Jančovičová, S European Hodophilus (Clavariaceae, Agaricales) species with yellow stipe Journal Article Mycological Progress, 17 (9), pp. 1097-1111, 2018, ISSN: ISSN: 1861-8952 . @article{Adamčík2018, title = {European Hodophilus (Clavariaceae, Agaricales) species with yellow stipe }, author = {S. Adamčík and B. Dima and K. Adamčíková and T. Læssøe and P.A. Moreau and A. Vizzini and S. Jančovičová}, doi = {https://doi.org/10.1007/s11557-018-1418-1}, issn = {ISSN: 1861-8952 }, year = {2018}, date = {2018-06-29}, journal = {Mycological Progress}, volume = {17}, number = {9}, pages = {1097-1111}, abstract = {Phylogenetic reconstruction of Hodophilus species with a yellow colour on the stipe based on nrITS, nrLSU and rpb2 sequences revealed six European species. All these species correspond to the widely accepted European concept of a single species Hodophilus micaceus. Four of these species are described and illustrated. H. micaceus and H. phaeoxanthus are recognised as two separate species and H. albofloccipes as a synonym of the latter. Two species, H. anatinus and H. cambriensis, are described as new. Possible endemism of H. micaceus and H. cambriensis to the British Isles is discussed. All analysed North American samples represent different species to those found in Europe. The North American species Hygrophorus rugulosus is combined in the genus Hodophilus. The preliminary key uses position and development of the yellow colour during maturation as themost important distinguishing character.The presence of the yellow colour is discussed as a possible synapomorphic character.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Phylogenetic reconstruction of Hodophilus species with a yellow colour on the stipe based on nrITS, nrLSU and rpb2 sequences revealed six European species. All these species correspond to the widely accepted European concept of a single species Hodophilus micaceus. Four of these species are described and illustrated. H. micaceus and H. phaeoxanthus are recognised as two separate species and H. albofloccipes as a synonym of the latter. Two species, H. anatinus and H. cambriensis, are described as new. Possible endemism of H. micaceus and H. cambriensis to the British Isles is discussed. All analysed North American samples represent different species to those found in Europe. The North American species Hygrophorus rugulosus is combined in the genus Hodophilus. The preliminary key uses position and development of the yellow colour during maturation as themost important distinguishing character.The presence of the yellow colour is discussed as a possible synapomorphic character. |
 | Ondrušková, E; Jánošíková, Z; Adamčík, S; Kádasi-Horáková, M; Rákusová-Sládková, D; Adamčíková, K Needle blight caused by Dothistroma pini in Slovakia: distribution, host range and mating types Journal Article Scandinavian Journal of Forest Research, 33 (7), pp. 650-656, 2018, ISSN: 1651-1891. @article{Ondrušková2018, title = {Needle blight caused by Dothistroma pini in Slovakia: distribution, host range and mating types}, author = {E. Ondrušková and Z. Jánošíková and S. Adamčík and M. Kádasi-Horáková and D. Rákusová-Sládková and K. Adamčíková}, doi = {10.1080/02827581.2018.1482954}, issn = {1651-1891}, year = {2018}, date = {2018-06-06}, journal = {Scandinavian Journal of Forest Research}, volume = {33}, number = {7}, pages = {650-656}, abstract = {Dothistroma needle blight (DNB) has been observed in Slovakia during the last two decades. Up until 2017, Dothistroma septosporum has only been detected and molecularly confirmed to cause DNB in Slovakia. Here, we report the detection of Dothistroma pini at six localities around Slovakia, representing different plantation types. Four pine species (Pinus sylvestris, P. nigra, P. mugo and P. jeffreyi) were confirmed as hosts of D. pini in Slovakia, of which only P. mugo has been previously reported as host in Slovakia. Three gene regions (ITS, EF1 –α, and ß-tubulin) of each of the 13 isolates were sequenced and assigned as D. pini. Based on ITS sequences, the studied isolates represent the haplotypes Dp_HAP.1, Dp_HAP.2. Both mating types were detected but at different localities. Our results suggest that in addition to D. septosporum, D. pini may contribute to DNB also in Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) has been observed in Slovakia during the last two decades. Up until 2017, Dothistroma septosporum has only been detected and molecularly confirmed to cause DNB in Slovakia. Here, we report the detection of Dothistroma pini at six localities around Slovakia, representing different plantation types. Four pine species (Pinus sylvestris, P. nigra, P. mugo and P. jeffreyi) were confirmed as hosts of D. pini in Slovakia, of which only P. mugo has been previously reported as host in Slovakia. Three gene regions (ITS, EF1 –α, and ß-tubulin) of each of the 13 isolates were sequenced and assigned as D. pini. Based on ITS sequences, the studied isolates represent the haplotypes Dp_HAP.1, Dp_HAP.2. Both mating types were detected but at different localities. Our results suggest that in addition to D. septosporum, D. pini may contribute to DNB also in Slovakia. |
 | Jánošíková-Hečková, Z; Ondrušková, E; Barta, M; Ostrovský, R; Kádasi-Horáková, M; Pastirčáková, K; Kobza, M; Adamčíková, K The hosts and geographic range of Dothistroma needle blight in Slovakia Journal Article Forest Pathology, 48 (3), pp. e12421, 2018, ISSN: 1437-4781. @article{Jánošíková-Hečková2018, title = {The hosts and geographic range of Dothistroma needle blight in Slovakia}, author = {Z. Jánošíková-Hečková and E. Ondrušková and M. Barta and R. Ostrovský and M. Kádasi-Horáková and K. Pastirčáková and M. Kobza and K. Adamčíková }, url = {https://onlinelibrary.wiley.com/doi/abs/10.1111/efp.12421}, doi = {10.1111/efp.12421}, issn = {1437-4781}, year = {2018}, date = {2018-02-15}, journal = {Forest Pathology}, volume = {48}, number = {3}, pages = {e12421}, abstract = {The occurrence and distribution of Dothistroma needle blight (DNB) were studied in 2014–2017 around Slovakia. A total of 84 localities, both native and planted, were investigated, and the presence of DNB was confirmed in 73 of them. In all positive locations, symptoms typical of DNB were observed and the Dothistroma species was confirmed using species-specific primers either from fungal cultures or directly from needles. Both Dothistroma species—D. septosporum and D. pini—were identified. Both species occurred together in 29 locations, only D. septosporum in 42 and only D. pini in two locations. The host range of D. septosporum included 10 pine species and two spruce species. The host range of D. pini comprised the same number of pine hosts but only one spruce species. Five pine hosts, P. aristata, P. coulteri, P. densiflora, P. jeffreyi, P. × schwerinii, and one spruce host P. abies are new hosts species of D. pini. P. densiflora and Picea pungens have earlier been reported to be susceptible for DNB. In this study, D. septosporum was found from both tree species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The occurrence and distribution of Dothistroma needle blight (DNB) were studied in 2014–2017 around Slovakia. A total of 84 localities, both native and planted, were investigated, and the presence of DNB was confirmed in 73 of them. In all positive locations, symptoms typical of DNB were observed and the Dothistroma species was confirmed using species-specific primers either from fungal cultures or directly from needles. Both Dothistroma species—D. septosporum and D. pini—were identified. Both species occurred together in 29 locations, only D. septosporum in 42 and only D. pini in two locations. The host range of D. septosporum included 10 pine species and two spruce species. The host range of D. pini comprised the same number of pine hosts but only one spruce species. Five pine hosts, P. aristata, P. coulteri, P. densiflora, P. jeffreyi, P. × schwerinii, and one spruce host P. abies are new hosts species of D. pini. P. densiflora and Picea pungens have earlier been reported to be susceptible for DNB. In this study, D. septosporum was found from both tree species. |
2017 |
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 | Jančovičová, S; Adamčík, S; Looney, B P; Caboň, M; Čaplovičová, M; Kopáni, M; Pennycook, S R; Adamčíková, K Delimitation of European Crepidotus stenocystis as different from the North American species C. brunnescens (Crepidotaceae, Agariccales) Journal Article Phytotaxa, 328 (2), pp. 127-139, 2017, ISSN: 1179-3163. @article{Jančovičová2017, title = {Delimitation of European Crepidotus stenocystis as different from the North American species C. brunnescens (Crepidotaceae, Agariccales)}, author = {S. Jančovičová and S. Adamčík and B.P. Looney and M. Caboň and M. Čaplovičová and M. Kopáni and S.R. Pennycook and K. Adamčíková}, doi = {https://doi.org/10.11646/phytotaxa.328.2.3}, issn = {1179-3163}, year = {2017}, date = {2017-11-17}, journal = {Phytotaxa}, volume = {328}, number = {2}, pages = {127-139}, abstract = {Crepidotus stenocystis and C. brunnescens are morphologically similar species defined by globose to subglobose spores, the presence of clamp connections in all tissues and bottle-like or flask-like cheilocystidia. They are also similar in the pileal aspect which is hygrophanous, glabrous or white-fibrillose, at first white but becoming brownish with age. Each are described from separate continents and have only been reported from their respective continents, C. stenocystis from Europe and C. brunnescens from North America. The phylogenetic analysis of IT S and LSU nrDNA regions from original type material confirms the existence of two distinct species, C. brunnescens that is more closely related to C. malachioides, and C. stenocystis that is more closely related to C. applanatus. Crepidotus stenocystis differs from C. brunnescens by its more prominent spore ornamentation and longer cheilocystidia that are frequently narrowly utriform. Based on the studied material and published data, it seems that C. stenocystis is distributed throughout all of Europe and does not occur in North America, whereas C. brunnescens is only known from Michigan in the USA.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Crepidotus stenocystis and C. brunnescens are morphologically similar species defined by globose to subglobose spores, the presence of clamp connections in all tissues and bottle-like or flask-like cheilocystidia. They are also similar in the pileal aspect which is hygrophanous, glabrous or white-fibrillose, at first white but becoming brownish with age. Each are described from separate continents and have only been reported from their respective continents, C. stenocystis from Europe and C. brunnescens from North America. The phylogenetic analysis of IT S and LSU nrDNA regions from original type material confirms the existence of two distinct species, C. brunnescens that is more closely related to C. malachioides, and C. stenocystis that is more closely related to C. applanatus. Crepidotus stenocystis differs from C. brunnescens by its more prominent spore ornamentation and longer cheilocystidia that are frequently narrowly utriform. Based on the studied material and published data, it seems that C. stenocystis is distributed throughout all of Europe and does not occur in North America, whereas C. brunnescens is only known from Michigan in the USA. |
 | Adamčík, S; Jančovičová, S; Looney, B P; Adamčíková, K; Griffith, G W; Læssøe, T; Moreau, P A; Vizzini, A; Matheny, P B Hodophilus (Clavariaceae, Agaricales) species with dark dots on the stipe: more than one species in Europe Journal Article Mycological Progress, 16 (8), pp. 811–821, 2017, ISSN: 1861-8952 . @article{Adamčík2017b, title = {Hodophilus (Clavariaceae, Agaricales) species with dark dots on the stipe: more than one species in Europe}, author = {S. Adamčík and S. Jančovičová and B.P. Looney and K. Adamčíková and G. W. Griffith and T. Læssøe and P.A. Moreau and A. Vizzini and P. B. Matheny }, doi = {DOI 10.1007/s11557-017-1318-9}, issn = {1861-8952 }, year = {2017}, date = {2017-07-01}, journal = {Mycological Progress}, volume = {16}, number = {8}, pages = {811–821}, abstract = {Hodophilus atropunctus is traditionally defined as the only species of this genus with dark brown or black dots on the stipe. Multi-locus phylogenetic reconstruction recognised two distinct clades morphologically corresponding to this species concept. The limited morphological description in the protologue of H. atropunctus and absence of a type specimen were limitations in an assignment of this name to one of the recognised phylogenetic species. The emended species concept and the selection of a neotype are based on careful analyses of the colour of the basidiomata and how this changes during maturation and drying. The name H. atropunctus is assigned to the paler of the two species which also shows colour change across the pileus and along the length of the stipe when dry. The second darker species is described here as new, H. variabilipes, but only seven out of 14 collections examined belonging to this taxon had distinct dark coloured dots on the stipe surface.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Hodophilus atropunctus is traditionally defined as the only species of this genus with dark brown or black dots on the stipe. Multi-locus phylogenetic reconstruction recognised two distinct clades morphologically corresponding to this species concept. The limited morphological description in the protologue of H. atropunctus and absence of a type specimen were limitations in an assignment of this name to one of the recognised phylogenetic species. The emended species concept and the selection of a neotype are based on careful analyses of the colour of the basidiomata and how this changes during maturation and drying. The name H. atropunctus is assigned to the paler of the two species which also shows colour change across the pileus and along the length of the stipe when dry. The second darker species is described here as new, H. variabilipes, but only seven out of 14 collections examined belonging to this taxon had distinct dark coloured dots on the stipe surface. |
 | Kádasi-Horáková, M; Adamčíková, K; Pastirčáková, K; Longauerová, V; Maľová, M Natural infection of Fraxinus angustifolia by Hymenoscyphus fraxineus in Slovakia Journal Article Baltic Forestry, 23 (1), pp. 52-55, 2017, ISSN: 2029-9230. @article{M.2017b, title = {Natural infection of \textit{Fraxinus angustifolia} by \textit{Hymenoscyphus fraxineus} in Slovakia}, author = {M. Kádasi-Horáková and K. Adamčíková and K. Pastirčáková and V. Longauerová and M. Maľová}, url = {https://www.balticforestry.mi.lt/bf/PDF_Articles/2017-23%5B1%5D/Baltic%20Forestry%202017.1_052-055.pdf}, issn = {2029-9230}, year = {2017}, date = {2017-04-07}, journal = {Baltic Forestry}, volume = {23}, number = {1}, pages = {52-55}, abstract = {The fungus Hymenoscyphus fraxineus is responsible for dieback of common ash (Fraxinus excelsior) and in some parts of Europe also of narrow-leaved ash (F. angustifolia). The first symptoms of ash dieback have been recorded on F. excelsior in Slovakia since 2004. This study reports about the first natural occurrence of H. fraxineus on F. angustifolia in Slovakia. The field investigation was carried out in 2014. The segments of diseased shoots and last year’s petioles were collected in clonal seed orchard situated in southwest part of the country. The fungus was isolated from infected host tissue and identified using molecular techniques (DNA extraction from pure cultures and apothecia, conventional PCR).}, keywords = {}, pubstate = {published}, tppubtype = {article} } The fungus Hymenoscyphus fraxineus is responsible for dieback of common ash (Fraxinus excelsior) and in some parts of Europe also of narrow-leaved ash (F. angustifolia). The first symptoms of ash dieback have been recorded on F. excelsior in Slovakia since 2004. This study reports about the first natural occurrence of H. fraxineus on F. angustifolia in Slovakia. The field investigation was carried out in 2014. The segments of diseased shoots and last year’s petioles were collected in clonal seed orchard situated in southwest part of the country. The fungus was isolated from infected host tissue and identified using molecular techniques (DNA extraction from pure cultures and apothecia, conventional PCR). |
 | Ondrušková, E; Hečková, Z; Kádasi-Horáková, M; Koltay, A; Ostrovský, R; Pažitný, J; Adamčíková, K Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia Journal Article European Journal of Plant Pathology, 148 (2), pp. 283-294, 2017, ISSN: 0929-1873. @article{Ondrušková2017, title = {Distribution and characterization of Dothistroma needle blight pathogens on Pinus mugo in Slovakia}, author = {E. Ondrušková and Z. Hečková and M. Kádasi-Horáková and A. Koltay and R. Ostrovský and J. Pažitný and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s10658-016-1088-2}, doi = {10.1007/s10658-016-1088-2}, issn = {0929-1873}, year = {2017}, date = {2017-01-01}, journal = {European Journal of Plant Pathology}, volume = {148}, number = {2}, pages = {283-294}, abstract = {The occurrence and distribution of Dothistroma needle blight (DNB) on Pinus mugo was studied in 2014–2015 around the Slovakia. In total, 42 localities were investigated both native and planted ones. Symptoms of DNB were observed on 35 localities only on planted shrubs. All these 35 localities are new P. mugo DNB stands. No DNB symptoms were observed in natural and naturally regenerated plantations. DNAwas extracted from a total of 236 isolates and eight needle samples. Based on the ITS-rDNA comparisons and using species specific primers, both pathogenic Dothistroma species were detected: D. septosporum and D. pini. Isolates of D. septosporum had ITS sequences identical to D. septosporum from Europe and both mating types were identified with slight predominance of MAT2. The ratio of D. septosporum mating types varies significantly between sites, ranging from an equal proportion of each mating type to single mating type populations. D. pini ITS sequence grouped with D. pini from Ukraine, Russia and Switzerland and only MAT2 was found.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The occurrence and distribution of Dothistroma needle blight (DNB) on Pinus mugo was studied in 2014–2015 around the Slovakia. In total, 42 localities were investigated both native and planted ones. Symptoms of DNB were observed on 35 localities only on planted shrubs. All these 35 localities are new P. mugo DNB stands. No DNB symptoms were observed in natural and naturally regenerated plantations. DNAwas extracted from a total of 236 isolates and eight needle samples. Based on the ITS-rDNA comparisons and using species specific primers, both pathogenic Dothistroma species were detected: D. septosporum and D. pini. Isolates of D. septosporum had ITS sequences identical to D. septosporum from Europe and both mating types were identified with slight predominance of MAT2. The ratio of D. septosporum mating types varies significantly between sites, ranging from an equal proportion of each mating type to single mating type populations. D. pini ITS sequence grouped with D. pini from Ukraine, Russia and Switzerland and only MAT2 was found. |
2016 |
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 | Adamčík, S; Jančovičová, S; Looney, B P; Adamčíková, K; Birkebak, J M; Moreau, P A; Vizziny, A; Matheny, P B Circumscription of species in the Hodophilus foetens complex (Clavariaceae, Agaricales) in Europe Journal Article Mycological Progress, 16 (1), pp. 47–62, 2016, ISSN: 1617-416X. @article{Adamčík2017, title = {Circumscription of species in the Hodophilus foetens complex (Clavariaceae, Agaricales) in Europe}, author = {S. Adamčík and S. Jančovičová and B.P. Looney and K. Adamčíková and J.M. Birkebak and P.A. Moreau and A. Vizziny and P.B. Matheny}, doi = {DOI 10.1007/s11557-016-1249-x}, issn = {1617-416X}, year = {2016}, date = {2016-12-12}, journal = {Mycological Progress}, volume = {16}, number = {1}, pages = {47–62}, abstract = {Four European Hodophilus species with an odour similar to naphthalene, a strong unpleasant odour similar to that of mothballs, are recognized based on sequence and/or morphological data. The traditional concept defines Ho. foetens as the only Hodophilus species with a naphthalene odour in Europe. This name is now assigned to one of the studied species based on morphological examination of the holotype specimen. A recently collected specimen is proposed as the epitype. The other three species with a naphthalene odour are described here as new: Ho. pallidus, Ho. subfoetens and Ho. tenuicystidiatus. They are distinguishable in the field based on a combination of lamellae number and colour of basidiomata. All four species are grouped in the Ho. foetens superclade, one of two superclades, together with the Ho. micaceus superclade, in the genus Hodophilus. All are different species from North American taxa with a naphthalene-like odour recognised in a previous study. The Ho. foetens superclade also includes one species identified as Ho. atropunctus that does not have a distinctive odour. The type collection of Ho. albofloccipes, a recently described European species with a naphthalene odour, is placed together with some collections without a distinctive odour in the Ho. micaceus superclade.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Four European Hodophilus species with an odour similar to naphthalene, a strong unpleasant odour similar to that of mothballs, are recognized based on sequence and/or morphological data. The traditional concept defines Ho. foetens as the only Hodophilus species with a naphthalene odour in Europe. This name is now assigned to one of the studied species based on morphological examination of the holotype specimen. A recently collected specimen is proposed as the epitype. The other three species with a naphthalene odour are described here as new: Ho. pallidus, Ho. subfoetens and Ho. tenuicystidiatus. They are distinguishable in the field based on a combination of lamellae number and colour of basidiomata. All four species are grouped in the Ho. foetens superclade, one of two superclades, together with the Ho. micaceus superclade, in the genus Hodophilus. All are different species from North American taxa with a naphthalene-like odour recognised in a previous study. The Ho. foetens superclade also includes one species identified as Ho. atropunctus that does not have a distinctive odour. The type collection of Ho. albofloccipes, a recently described European species with a naphthalene odour, is placed together with some collections without a distinctive odour in the Ho. micaceus superclade. |
 | Bulman, L S; Bradshaw, R E; Fraser, S; Martín‐García, J; Barnes, I; Musolin, D L; Porta, La N; Woods, A J; Diez, J J; Koltay, A; Drenkhan, R; Ahumada, R; Poljakovic‐Pajnik, L; Queloz, V; Piškur, B; Doğmuş‐Lehtijärvi, H T; Chira, D; Tomešová‐Haataja, V; Georgieva, M; Jankovský, L; Anselmi, N; Markovskaja, S; Papazova‐Anakieva, I; Sotirovski, K; Lazarević, J; Adamčíková, K; Boroń, P; Bragança, H; Vettraino, A M; Selikhovkin, A V; Bulgakov, T S; Tubby, K A worldwide perspective on the management and control of Dothistroma needle blight Journal Article Forest Pathology : Journal de pathologie forestiere, 46 (5), pp. 472-488, 2016, ISSN: 1437-4781. @article{Bulman2016, title = {A worldwide perspective on the management and control of Dothistroma needle blight}, author = {L. S. Bulman and R. E. Bradshaw and S. Fraser and J. Martín‐García and I. Barnes and D. L. Musolin and N. La Porta and A. J. Woods and J. J. Diez and A. Koltay and R. Drenkhan and R. Ahumada and L. Poljakovic‐Pajnik and V. Queloz and B. Piškur and H. T. Doğmuş‐Lehtijärvi and D. Chira and V. Tomešová‐Haataja and M. Georgieva and L. Jankovský and N. Anselmi and S. Markovskaja and I. Papazova‐Anakieva and K. Sotirovski and J. Lazarević and K. Adamčíková and P. Boroń and H. Bragança and A. M. Vettraino and A. V. Selikhovkin and T. S. Bulgakov and K. Tubby }, doi = {https://doi.org/10.1111/efp.12305}, issn = {1437-4781}, year = {2016}, date = {2016-09-22}, journal = {Forest Pathology : Journal de pathologie forestiere}, volume = {46}, number = {5}, pages = {472-488}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
 | Adamčík, S; Looney, B P; Birkebak, J M; Jančovičová, S; Adamčíková, K; Marhold, K; Matheny, P B Circumscription of species of Hodophilus (Clavariaceae, Agaricales) in North America with naphthalene odours Journal Article Botany, 94 (10), pp. 941-956, 2016, ISSN: 1916-2804. @article{ADAMČÍK2016, title = {Circumscription of species of Hodophilus (Clavariaceae, Agaricales) in North America with naphthalene odours}, author = {S. Adamčík and B.P. Looney and J.M. Birkebak and S. Jančovičová and K. Adamčíková and K. Marhold and P.B. Matheny}, doi = {dx.doi.org/10.1139/cjb-2016-0091}, issn = {1916-2804}, year = {2016}, date = {2016-06-14}, journal = {Botany}, volume = {94}, number = {10}, pages = {941-956}, abstract = {Five North American Hodophilus species with naphthalene-like odours are now recognized based on sequence and (or) morphological data and molecular annotation of type collections. Two well-supported eastern North American species do not match any of the studied types and are described here as new: Hodophilus hesleri and Hodophilus smithii. The previously described Hodophilus paupertinus is found to represent an autonomous species and appears restricted to western North America. Hodophilus subfuscescens is found to be an independent lineage in eastern North America. A morphological type study of Hodophilus peckianus shows that it is a distinct species and not represented among recent collections. Multilocus phylogenetic analyses of European and North American material of species with naphthalene odours reveal no species with transatlantic distributions. Overall, Hodophilus comprises two superclades (the Hodophilus foetens superclade and the Hodophilus micaceus superclade) and 16 terminal clades that correspond to phylogenetic species. This study introduces a new approach for morphological delimitation of agaricoid Clavariaceae combining shape and dimensions of particular elements in the pileipellis and caulocystidia. All previously described taxa included in this study, which were previously treated in the genera Hygrophorus, Camarophyllopsis, or Hygrotrama, are formally transferred to Hodophilus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five North American Hodophilus species with naphthalene-like odours are now recognized based on sequence and (or) morphological data and molecular annotation of type collections. Two well-supported eastern North American species do not match any of the studied types and are described here as new: Hodophilus hesleri and Hodophilus smithii. The previously described Hodophilus paupertinus is found to represent an autonomous species and appears restricted to western North America. Hodophilus subfuscescens is found to be an independent lineage in eastern North America. A morphological type study of Hodophilus peckianus shows that it is a distinct species and not represented among recent collections. Multilocus phylogenetic analyses of European and North American material of species with naphthalene odours reveal no species with transatlantic distributions. Overall, Hodophilus comprises two superclades (the Hodophilus foetens superclade and the Hodophilus micaceus superclade) and 16 terminal clades that correspond to phylogenetic species. This study introduces a new approach for morphological delimitation of agaricoid Clavariaceae combining shape and dimensions of particular elements in the pileipellis and caulocystidia. All previously described taxa included in this study, which were previously treated in the genera Hygrophorus, Camarophyllopsis, or Hygrotrama, are formally transferred to Hodophilus. |
2015 |
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 | Adamčíková, K; Kádasi-Horáková, M; Jankovský, L; Havrdová, L Identification of Hymenoscyphus fraxineus, the causal agent of ash dieback in Slovakia Journal Article Biologia, 70 (5), pp. 559–564, 2015. @article{K.2015, title = {Identification of \textit{Hymenoscyphus fraxineus}, the causal agent of ash dieback in Slovakia}, author = {K. Adamčíková and M. Kádasi-Horáková and L. Jankovský and L. Havrdová}, url = {http://www.degruyter.com/view/j/biolog.2015.70.issue-5/biolog-2015-0075/biolog-2015-0075.xml}, doi = {10.1515/biolog-2015-0075}, year = {2015}, date = {2015-06-23}, journal = {Biologia}, volume = {70}, number = {5}, pages = {559–564}, abstract = {Symptoms of ash dieback have been recorded in Slovakia since 2004. The field sampling was carried out in 2013, included 59 segments of shoots and 10 and more petioles per locality from four localities. The causal agent of ash dieback, the hyphomycete Chalara fraxinea T. Kowalski, was isolated from Fraxinus excelsior L. from seven localities in Slovakia. The morphology of C. fraxinea isolates and the teleomorph Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz, Hosoya are described and ITS sequences are provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Symptoms of ash dieback have been recorded in Slovakia since 2004. The field sampling was carried out in 2013, included 59 segments of shoots and 10 and more petioles per locality from four localities. The causal agent of ash dieback, the hyphomycete Chalara fraxinea T. Kowalski, was isolated from Fraxinus excelsior L. from seven localities in Slovakia. The morphology of C. fraxinea isolates and the teleomorph Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz, Hosoya are described and ITS sequences are provided. |
 | Adamčíková, K; Ondrušková, E; Kádasi-Horáková, M; Botu, M; Kobza, M; Achim, G Distribution and population structure of the chestnut blight fungus in Romania Journal Article Plant Protection Science, 51 (3), pp. 141-149, 2015. @article{Adamčíková2015, title = {Distribution and population structure of the chestnut blight fungus in Romania}, author = {K. Adamčíková and E. Ondrušková and M. Kádasi-Horáková and M. Botu and M. Kobza and G. Achim}, url = {http://www.agriculturejournals.cz/publicFiles/157004.pdf}, doi = {10.17221/52/2014-PPS}, year = {2015}, date = {2015-01-01}, journal = {Plant Protection Science}, volume = {51}, number = {3}, pages = {141-149}, abstract = {The occurrence of chestnut blight (Cryphonectria parasitica) was studied in 2011-2012 at 13 locations in the main chestnut growing areas of Romania. Infections were detected at four localities. The symptoms and the fungus were detected on European chestnut (four localities) and also on oak trees (two localities). A total of 89 isolates of C. parasitica were isolated and characterised. Based on canker and isolate morphology (culture morphology and the Bavendamm test), both virulent and hypovirulent samples were isolated; hypovirulent isolates were found at only one locality. Two vegetative compatibility types corresponding to EU-12 and EU-2 were identified among isolates. Both mating types were found, with a dominance of MAT-1 in southern Romania and MAT-2 in northern Romania.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The occurrence of chestnut blight (Cryphonectria parasitica) was studied in 2011-2012 at 13 locations in the main chestnut growing areas of Romania. Infections were detected at four localities. The symptoms and the fungus were detected on European chestnut (four localities) and also on oak trees (two localities). A total of 89 isolates of C. parasitica were isolated and characterised. Based on canker and isolate morphology (culture morphology and the Bavendamm test), both virulent and hypovirulent samples were isolated; hypovirulent isolates were found at only one locality. Two vegetative compatibility types corresponding to EU-12 and EU-2 were identified among isolates. Both mating types were found, with a dominance of MAT-1 in southern Romania and MAT-2 in northern Romania. |
2014 |
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 | Pastirčáková, K; Pastirčák, M; Adamčiková, K; Bouznad, Z; Kedad, A; El_Guilli, M; Diminić, D; Hofte, M Global distribution of Erysiphe platani: new records, teleomorph formation and re-examination of herbarium collections Journal Article Cryptogamie, Mycologie, 35 (2), pp. 163-176, 2014, ISSN: 0181-1584. @article{K.2014, title = {Global distribution of \textit{Erysiphe platani}: new records, teleomorph formation and re-examination of herbarium collections}, author = {K. Pastirčáková and M. Pastirčák and K. Adamčiková and Z. Bouznad and A. Kedad and M. El_Guilli and D. Diminić and M. Hofte}, url = {http://www.bioone.org/doi/abs/10.7872/crym.v35.iss2.2014.163?journalCode=crym}, doi = {10.7872/crym.v35.iss2.2014.163}, issn = {0181-1584}, year = {2014}, date = {2014-06-01}, journal = {Cryptogamie, Mycologie}, volume = {35}, number = {2}, pages = {163-176}, abstract = {A global survey of the spread of the Platanus powdery mildew, Erysiphe platani, has been carried out. E. platani teleomorph formation was recorded in countries where the fungus anamorph has been present for several years. The first findings of chasmothecia were recorded in Austria, Czech Republic, France, Italy and Slovakia. New records of E. platani (including the teleomorph) were found in Belgium, Croatia and Denmark. The occurrence of this fungus in Sweden and in two countries of North Africa (Algeria and Morocco) was confirmed. Descriptions of morphological features, illustrations, and worldwide distribution of E. platani are provided. Herbarium collections of powdery mildews on Platanus spp. were re-examined and revised. The occurrence of Phyllactinia guttata on Platanus is discussed and questioned.}, keywords = {}, pubstate = {published}, tppubtype = {article} } A global survey of the spread of the Platanus powdery mildew, Erysiphe platani, has been carried out. E. platani teleomorph formation was recorded in countries where the fungus anamorph has been present for several years. The first findings of chasmothecia were recorded in Austria, Czech Republic, France, Italy and Slovakia. New records of E. platani (including the teleomorph) were found in Belgium, Croatia and Denmark. The occurrence of this fungus in Sweden and in two countries of North Africa (Algeria and Morocco) was confirmed. Descriptions of morphological features, illustrations, and worldwide distribution of E. platani are provided. Herbarium collections of powdery mildews on Platanus spp. were re-examined and revised. The occurrence of Phyllactinia guttata on Platanus is discussed and questioned. |
![Príčiny hromadného hynutia gaštana jedlého [Causal agent of European chestnut blight]](https://ife.sk/wp-content/uploads/2016/10/IFE_adamcikova_katarina_article_08.jpg) | Juhásová, G; Adamčíková, K; Kobza, M; Ostrovský, R Príčiny hromadného hynutia gaštana jedlého [Causal agent of European chestnut blight] Journal Article Zahradnictví : časopis profesionálních zahradníků, 13 (7), pp. 48-51, 2014, ISSN: 1213-7596. @article{Juhásová2014, title = {Príčiny hromadného hynutia gaštana jedlého [Causal agent of European chestnut blight]}, author = {G. Juhásová and K. Adamčíková and M. Kobza and R. Ostrovský}, issn = {1213-7596}, year = {2014}, date = {2014-01-01}, journal = {Zahradnictví : časopis profesionálních zahradníků}, volume = {13}, number = {7}, pages = {48-51}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
 | Bolvanský, M; Ostrovský, R; Kobza, M; Adamčíková, K; Pažitný, J; Juhásová, G; Kajaba, P Spread of chestnut blight in Slovakia in relation to the site topography and climatic characteristics Inproceedings Acta Horticulturae: Proceedings of the second European congress on chestnut : Debrecen, Hungary, Baia Mare, Romania, Modry Kamen, Slovakia, October 9-12, 2013. , pp. 35-42, ISHS, 2014, ISBN: 978 94 6261 032 3. @inproceedings{Bolvanský2014, title = {Spread of chestnut blight in Slovakia in relation to the site topography and climatic characteristics}, author = {M. Bolvanský and R. Ostrovský and M. Kobza and K. Adamčíková and J. Pažitný and G. Juhásová and P. Kajaba}, isbn = {978 94 6261 032 3}, year = {2014}, date = {2014-01-01}, booktitle = {Acta Horticulturae: Proceedings of the second European congress on chestnut : Debrecen, Hungary, Baia Mare, Romania, Modry Kamen, Slovakia, October 9-12, 2013. }, number = {1043}, pages = {35-42}, publisher = {ISHS}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
![Gaštan jedlý na Slovensku a v Európe : pestovanie, ochrana, variabilita a využitie [European chestnut in Slovakia : growing, protection, use and genetic variability]](http://ife.sk/wp-content/uploads/2016/10/IFE_adamcikova_katarina_article_08.jpg) | Adamčíková, K; Kobza, M; Juhásová, G; Ondrušková, E; Bolvanský, M; Kádasi-Horáková, M Gaštan jedlý na Slovensku a v Európe : pestovanie, ochrana, variabilita a využitie [European chestnut in Slovakia : growing, protection, use and genetic variability] Book Garamond, Nitra, 2014, ISBN: 978-80-89408-18-4. @book{Adamčíková2014, title = {Gaštan jedlý na Slovensku a v Európe : pestovanie, ochrana, variabilita a využitie [European chestnut in Slovakia : growing, protection, use and genetic variability]}, author = {K. Adamčíková and M. Kobza and G. Juhásová and E. Ondrušková and M. Bolvanský and M. Kádasi-Horáková}, isbn = {978-80-89408-18-4}, year = {2014}, date = {2014-01-01}, volume = {1}, pages = {155}, publisher = {Garamond}, address = {Nitra}, keywords = {}, pubstate = {published}, tppubtype = {book} } |
 | Bolvanský, M; Adamčíková, K; Kobza, M Screening resistance to chestnut blight in young chestnut trees derived from Castanea sativa × C. crenata hybrids Journal Article Folia Oecologica, 41 (1), pp. 1-7, 2014, ISSN: 1336-5266. @article{Bolvanský2014c, title = {Screening resistance to chestnut blight in young chestnut trees derived from \textit{Castanea sativa} × \textit{C. crenata} hybrids}, author = {M. Bolvanský and K. Adamčíková and M. Kobza}, url = {http://ife.sk/wp-content/uploads/2016/10/FO_v41_iss1_1to7.pdf}, issn = {1336-5266}, year = {2014}, date = {2014-01-01}, journal = {Folia Oecologica}, volume = {41}, number = {1}, pages = {1-7}, abstract = {In 2010, 2011 and 2012 four trials were carried out to prove a reliability of the new screening method for resistance to chestnut blight caused by Cryphonectria parasitica. In the selected trees of hybrid origin (C. sativa × C. crenata)) 10 cm long cut-branch sections were inoculated with mycelia of three virulent isolates and one hypovirulent isolate (in two last trials) and cultivated for 7 days in the dark at 25 °C with 95% humidity. Then the bark of branch segments was peeled off and the size of necrotic lesions formed on the wood tissue around the inoculation place was measured. The size of necrotic areas varied by sampling date, type of fungus strains and tested trees. In all three summer trials necrotic lesions were larger than lesions in a spring trial of 2011, in which still dormant stem sections were used. Unlike the summer trials in the spring trial higher differences in the size of necrotic lesions among tested trees and among used virulent strains were observed. In the spring trial interactions between tested trees and fungal isolates were not so frequent like in summer trials where more trees exhibited different response to the same virulent strain. Majority of trees showed different susceptibility in particular trials. Observed high variation of reactions of tested trees to both virulent and hypovirulent isolates has pointed at the need to prove other screening methods, and to find such one, that would be highly effective to reveal an inherited resistance and/or a lower degree of susceptibility to chestnut blight.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In 2010, 2011 and 2012 four trials were carried out to prove a reliability of the new screening method for resistance to chestnut blight caused by Cryphonectria parasitica. In the selected trees of hybrid origin (C. sativa × C. crenata)) 10 cm long cut-branch sections were inoculated with mycelia of three virulent isolates and one hypovirulent isolate (in two last trials) and cultivated for 7 days in the dark at 25 °C with 95% humidity. Then the bark of branch segments was peeled off and the size of necrotic lesions formed on the wood tissue around the inoculation place was measured. The size of necrotic areas varied by sampling date, type of fungus strains and tested trees. In all three summer trials necrotic lesions were larger than lesions in a spring trial of 2011, in which still dormant stem sections were used. Unlike the summer trials in the spring trial higher differences in the size of necrotic lesions among tested trees and among used virulent strains were observed. In the spring trial interactions between tested trees and fungal isolates were not so frequent like in summer trials where more trees exhibited different response to the same virulent strain. Majority of trees showed different susceptibility in particular trials. Observed high variation of reactions of tested trees to both virulent and hypovirulent isolates has pointed at the need to prove other screening methods, and to find such one, that would be highly effective to reveal an inherited resistance and/or a lower degree of susceptibility to chestnut blight. |
2013 |
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![Diversity of microfungi on branches of Castanea sativa in Slovakia [Diverzita mikroskopických húb na konároch Castanea sativa na Slovensku]](http://ife.sk/wp-content/uploads/2016/10/g4646.png) | Adamčíková, K; Juhásová, G; Kobza, M; Ondrušková, E Diversity of microfungi on branches of Castanea sativa in Slovakia [Diverzita mikroskopických húb na konároch Castanea sativa na Slovensku] Journal Article Polish Botanical Journal, 58 (2), pp. 741-746, 2013, ISSN: 1641-8180. @article{Adamčíková2013, title = {Diversity of microfungi on branches of Castanea sativa in Slovakia [Diverzita mikroskopických húb na konároch Castanea sativa na Slovensku]}, author = {K. Adamčíková and G. Juhásová and M. Kobza and E. Ondrušková}, issn = {1641-8180}, year = {2013}, date = {2013-01-01}, journal = {Polish Botanical Journal}, volume = {58}, number = {2}, pages = {741-746}, abstract = {Localities in Castanea sativa Mill. plantations were visited in a study aimed at identifying the mycoflora of C. sativa in Slovakia [excluding Cryphonectria parasitica (Murr.), Barr for which much data is available]. Samples from chestnut tree branches and stems were examined visually and microscopically. Seven species of microfungi were recorded, three with their anamorphs. Coryneum modonium (Sacc.) Griffon & Maubl. and Phomopsis castaneae Woron. were the most common. Libertella quercina Tul. & C. Tul. was identified in both states (anamorph and teleomorph) at two new localities. The records of Gloniopsis praelonga (Schwein.) Underw. & Earle and Dothidotthia celtidis (Ellis & Everh.) M. E. Barr are the first for Slovakia. These species were rare, found only in one locality. Two microscopic fungi were detected: Valsa ambiens (Pers.) Fr. [Cytospora leucosperma (Pers.) Fr.] and Diplodina castaneae Prill. & Delacr.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Localities in Castanea sativa Mill. plantations were visited in a study aimed at identifying the mycoflora of C. sativa in Slovakia [excluding Cryphonectria parasitica (Murr.), Barr for which much data is available]. Samples from chestnut tree branches and stems were examined visually and microscopically. Seven species of microfungi were recorded, three with their anamorphs. Coryneum modonium (Sacc.) Griffon & Maubl. and Phomopsis castaneae Woron. were the most common. Libertella quercina Tul. & C. Tul. was identified in both states (anamorph and teleomorph) at two new localities. The records of Gloniopsis praelonga (Schwein.) Underw. & Earle and Dothidotthia celtidis (Ellis & Everh.) M. E. Barr are the first for Slovakia. These species were rare, found only in one locality. Two microscopic fungi were detected: Valsa ambiens (Pers.) Fr. [Cytospora leucosperma (Pers.) Fr.] and Diplodina castaneae Prill. & Delacr. |