Adamčíková Katarína
Plant Pathology and Mycology RG
Research Group Leader
Mgr. Katarína Adamčíková, PhD.
Slovak Academy of Sciences
Institute of Forest Ecology
Plant Pathology & Mycology Research Group
Akademická 2
949 01 Nitra
Phone: +421 37 6943 339
Email: katarina.adamcikova@ife.sk
Research Interests: fungal diseases of woody plants, biological control, mycology, diversity and distribution of pathogens, plant protection. Current research interest: chestnut blight disease, Dothistroma needle blight, ash dieback.
Education:
- PhD., Faculty of Agrobiology and Food Resources Slovak University of Agriculture in Nitra, Slovakia 2004 (plant protection)
- MSc., Faculty of Natural Sciences Comenius University Bratislava, Department of Plant Physiology, Slovak Republic 1997 (biology – plant physiology)
Academic employment:
- Institute of Forest Ecology of Slovak Academy of Sciences
- Senior researcher (since 2013)
- Head of Branch for Woody Plants Biology (since 2012)
- Scientific researcher – (2003 – 2012)
- PhD. student (1997 – 2003)
National projects:
- MVTS Ochrana mestských stromov (2021-2025, member of research team)
- VEGA 2/0132/22 Impact of climate change on the distribution of selected pathogens of Pinus sp. trees (2022-2025, deputy of project leader)
- VEGA 2/0050/22 Unraveling of functional diversity and ecological role of fungi of the family Clavariaceae (2022-2025, member of research team)
- VEGA 1/0346/22 What we (do not) know about the genus Crepidotus (Agaricomycotina, fungi), (2022-2025, member of research team)
- APVV-20-0257 Tree and country – influence of trees on diversity of soil microorganisms in agricultural land (2021-2025, member of research team)
- VEGA 2/0077/18 Identification, genetic variability a pathogenicity of economically important needle cast species on pines (2018-2021, project leader)
- VEGA 2/0062/18 Ash dieback: the causal agents and disease control strategy (2018-2021, member of research team)
- SK-FR-2017-0025 Spatial analyses in population genetic studies of tree pathogens (2018-2019, project leader)
- VEGA 2/0018/18 Systematics and phylogeny of the genus Dermoloma in Europe and North America (2018-2021, member of research team)
- APVV-15-0210 Distribution potential of different fungal groups in Europe (2016–2020, member of research team)
- VEGA 2/00143/15 Study of the effect of environmental variables on the incidence and spread of chestnut blight in European chestnut (Castanea sativa) in Slovakia and possibilities to control this disease (2015-2018, deputy of project leader)
- VEGA 2/0069/14 Biology, spread and diagnostics of harmful agent Dothistroma septosporum (Mycosphaerella pini), D. pini and other harmful agents of pines assimilation organs (2014-2017, deputy of project leader)
- VEGA 2/0071/14 Species diversity and biological characteristics of parasitic fungi associated with damage and withering of woody plants (2014-2017, member of research team)
- HUSK/1101/2.2.1/0230 Name of project: Our common natural resource: the European chestnut (2012-2014, project leader)
- APVV SK-MAD 0024-10 Study of genetic resources of selected nut crops in Slovakia and Romania (2011-2012, member of research team)
- VEGA 2/0149/10 Study of morphological, genetical and biological characteristics of selected genera of fungi from Coelomycetes, their pathogenicity and occurrence in different ecological conditions (2010-2013, member of research team)
- VEGA 2/0149/10 Study of the phenotypic and genetic variability and different susceptibility to the fungi diseases European chestnut (Castanea sativa Mill.) (2010-2013, member of research team)
International projects:
- Euphresco 2016-G-223 Lecanosticta – Brown spot disease of pines – spread in European forest ecosystems: impact on pines, predisposing and contributing factors, control (2017-2020, member of research team)
- COST FP 1403 Pine pitch canker – strategies for management of Gibberella circinata in greenhouses and forests (2015-2019)
- COST FP 1103 Fraxinus dieback in Europe: elaborating guidelines and strategies for sustainable management (2012-2016)
- COST FP 1102 Determining invasiveness and risk of Dothistroma (2011-2015)
List of my publications:
2025 |
|
 | Adamčíková, K; Kiran, M; Caboň, M; Matheny, B P; Sánchez-García, M; Arnolds, E; Caboňová, M; Corriol, G; Dima, B; Friebes, G; Griffith, G W; Grootmyers, D; Harries, D; Karich, A; Mešić, A; Mihaljevič, M; Moreau, P -A; Pošta, A; Shapkin, V; Tkalčec, Z; Vizzini, A; Vondrovicová, L; Adamčík, S; Jančovičová, S IMA Fungus, 16 , pp. e157337, 2025, ISSN: 2210-6359. @article{Adamčíková2025, title = {A phylogenetic and morphological study of the genus Dermoloma (Agaricales, Tricholomataceae) in Europe and North America exposes inefficiency of opportunistic species descriptions}, author = {K. Adamčíková and M. Kiran and M. Caboň and B. P. Matheny and M. Sánchez-García and E. Arnolds and M. Caboňová and G. Corriol and B. Dima and G. Friebes and G. W. Griffith and D. Grootmyers and D. Harries and A. Karich and A. Mešić and M. Mihaljevič and P.-A. Moreau and A. Pošta and V. Shapkin and Z. Tkalčec and A. Vizzini and L. Vondrovicová and S. Adamčík and S. Jančovičová}, doi = {DOI: 10.3897/imafungus.16.157337}, issn = {2210-6359}, year = {2025}, date = {2025-07-10}, journal = {IMA Fungus}, volume = {16}, pages = {e157337}, abstract = {Dermoloma is traditionally known as a small genus of agarics classified in the family Tricholomataceae. This study implemented a multilocus phylogeny of six DNA regions to recognize phylogenetic species within the genus. The species concept is reinforced by observations of well-defined morphological characters enhanced by long term sampling effort in Europe and North America. Thirty European Dermoloma species are described, including 16 new species from Europe and three from North American. These species are classified into two subgenera morphologically distinguished by spores with positive or negative amyloid reaction. A new genus Neodermoloma is introduced for the Dermoloma-like species N. campestre. Localized or continental-scale species endemicity was confirmed based on studied material, but more inclusive phylogenetic clustering supported a mixture of North American species among the European clades. Of the 22 names validly published from Europe prior to this study, 11 could be assigned to well-defined Dermoloma species recognized here. Of the remaining 11 names, two were considered representing Dermoloma species not recorded since their description, and nine were established as later synonyms of other species. Morphological studies of Dermoloma are challenging due to the relatively low number of characters suitable for identification of species. The majority of morphological characters showed continuous variation with high overlap throughout the genus. For this reason, species identification requires an awareness of morphological variability within species, and multiple distinguishing characters need to be combined, and furthermore, often a barcode sequence is needed for a certain identification. Stable isotope analysis in Dermoloma of δ13C and δ15N revealed an ecological signature similar to known CHEGD fungi, i.e. Clavariaceae and Hygrocybe s.l. This indicates that Dermoloma species are biotrophic but neither ectomycorrhizal nor saprotrophic and may form mutualistic root endophytic associations with vascular plants.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dermoloma is traditionally known as a small genus of agarics classified in the family Tricholomataceae. This study implemented a multilocus phylogeny of six DNA regions to recognize phylogenetic species within the genus. The species concept is reinforced by observations of well-defined morphological characters enhanced by long term sampling effort in Europe and North America. Thirty European Dermoloma species are described, including 16 new species from Europe and three from North American. These species are classified into two subgenera morphologically distinguished by spores with positive or negative amyloid reaction. A new genus Neodermoloma is introduced for the Dermoloma-like species N. campestre. Localized or continental-scale species endemicity was confirmed based on studied material, but more inclusive phylogenetic clustering supported a mixture of North American species among the European clades. Of the 22 names validly published from Europe prior to this study, 11 could be assigned to well-defined Dermoloma species recognized here. Of the remaining 11 names, two were considered representing Dermoloma species not recorded since their description, and nine were established as later synonyms of other species. Morphological studies of Dermoloma are challenging due to the relatively low number of characters suitable for identification of species. The majority of morphological characters showed continuous variation with high overlap throughout the genus. For this reason, species identification requires an awareness of morphological variability within species, and multiple distinguishing characters need to be combined, and furthermore, often a barcode sequence is needed for a certain identification. Stable isotope analysis in Dermoloma of δ13C and δ15N revealed an ecological signature similar to known CHEGD fungi, i.e. Clavariaceae and Hygrocybe s.l. This indicates that Dermoloma species are biotrophic but neither ectomycorrhizal nor saprotrophic and may form mutualistic root endophytic associations with vascular plants. |
 | Jánošíková, Z; Adamčíková, K; Ondrušková, E; Ostrovský, R; Woodward, S; Fraser, S Histological Analysis of Dothistroma septosporum Infection on Different Provenances of Pinus sylvestris Journal Article Forests, 16 , pp. 973, 2025, ISSN: 1999-4907. @article{Jánošíková2025, title = {Histological Analysis of Dothistroma septosporum Infection on Different Provenances of Pinus sylvestris}, author = {Z. Jánošíková and K. Adamčíková and E. Ondrušková and R. Ostrovský and S. Woodward and S. Fraser}, url = {http://ife.sk/wp-content/uploads/2020/09/fig-5.jpg}, doi = {10.3390/f16060973}, issn = {1999-4907}, year = {2025}, date = {2025-06-09}, journal = {Forests}, volume = {16}, pages = {973}, abstract = {Dothistroma needle blight (DNB) is one of the most significant diseases of conifers, causing premature defoliation, growth reduction, and, in extreme cases, mortality. Histological analysis was undertaken on inoculated seedlings of three different seed sources of Pinus sylvestris L. to investigate the process of infection and degradation of needle tissue on this host species. Seedlings were inoculated using a single spore isolate of Dothistroma septosporum (Doroguine) M. Morelet (D636) from northern Scotland. Mesophyll degradation in the needles occurred by four weeks after inoculation; collapse of mesophyll, bundle sheath tissues, and tracheids by five weeks; and eruption of fruiting bodies in near proximity to stomatal openings by six weeks. Significantly greater collapse of mesophyll during the early stages of infection occurred in the Austrian provenance compared with the United Kingdom provenance, although in the later stages of infection, this difference disappeared. Furthermore, disease severity, assessed as the proportion of needles with D. septosporum conidiomata on each tree, was not significantly different between seed sources.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is one of the most significant diseases of conifers, causing premature defoliation, growth reduction, and, in extreme cases, mortality. Histological analysis was undertaken on inoculated seedlings of three different seed sources of Pinus sylvestris L. to investigate the process of infection and degradation of needle tissue on this host species. Seedlings were inoculated using a single spore isolate of Dothistroma septosporum (Doroguine) M. Morelet (D636) from northern Scotland. Mesophyll degradation in the needles occurred by four weeks after inoculation; collapse of mesophyll, bundle sheath tissues, and tracheids by five weeks; and eruption of fruiting bodies in near proximity to stomatal openings by six weeks. Significantly greater collapse of mesophyll during the early stages of infection occurred in the Austrian provenance compared with the United Kingdom provenance, although in the later stages of infection, this difference disappeared. Furthermore, disease severity, assessed as the proportion of needles with D. septosporum conidiomata on each tree, was not significantly different between seed sources. |
 | Shapkin, V; Caboň, M; Kolařík, M; Adamčíková, K; Baldrian, P; Michalková, T; Větrovský, T; Adamčík, S Protein Coding Low-Copy rpb2 and ef1-α Regions Are Viable Fungal Metabarcoding DNA Markers Which Can Supplement ITS for Better Accuracy Journal Article Ecology and Evolution, 15 (e71352), 2025, ISSN: 2045-7758. @article{Shapkin2025, title = {Protein Coding Low-Copy rpb2 and ef1-α Regions Are Viable Fungal Metabarcoding DNA Markers Which Can Supplement ITS for Better Accuracy}, author = {V. Shapkin and M. Caboň and M. Kolařík and K. Adamčíková and P. Baldrian and T. Michalková and T. Větrovský and S. Adamčík}, doi = {10.1002/ece3.71352}, issn = {2045-7758}, year = {2025}, date = {2025-04-23}, journal = {Ecology and Evolution}, volume = {15}, number = {e71352}, abstract = {The nuclear ribosomal DNA Internal Transcribed Spacer (ITS) region is used as a universal fungal barcode marker, but often lacks a significant DNA barcoding gap between sister taxa. Here we tested the reliability of protein coding low-copy genes as alternative barcode markers. Mock communities of three unrelated agaric genera (Dermoloma, Hodophilus, and Russula) representing lineages of closely related species were sequenced by the Illumina platform targeting the ITS1, ITS2, the second largest subunit of RNA polymerase II gene (rpb2) and the transcription elongation factor 1-alpha gene (ef1-α) regions. Species representation and their relative abundances were similar across all tested barcode regions, despite a lower copy number in protein coding markers. ITS1 and ITS2 required more sophisticated sequence filtering because they produced a high number of chimeric sequences requiring reference-based chimera removal and had a higher number of sequence variants per species. Although clustering of filtered ITS sequences resulted in an average higher number of correctly clustered units at optimal similarity thresholds, these thresholds varied substantially among genera. Best-fitted thresholds of low-copy markers were more consistent across genera but frequently lacked species resolution due to low intraspecific variability. At some thresholds, we observed multiple species lumped together, and at the same time, species split into multiple partial clusters, which should be taken into consideration when assessing the best clustering thresholds and taxonomic identity of clusters. To achieve the best taxonomic resolution and improve species detection, we recommend combining different markers and applying additional reference-based sorting of clusters. The current availability of rpb2 and ef1-α reference sequences in public databases is far from being complete for all fungal groups, but a combined marker approach can be used for group-specific studies that can build reference data for their own purposes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The nuclear ribosomal DNA Internal Transcribed Spacer (ITS) region is used as a universal fungal barcode marker, but often lacks a significant DNA barcoding gap between sister taxa. Here we tested the reliability of protein coding low-copy genes as alternative barcode markers. Mock communities of three unrelated agaric genera (Dermoloma, Hodophilus, and Russula) representing lineages of closely related species were sequenced by the Illumina platform targeting the ITS1, ITS2, the second largest subunit of RNA polymerase II gene (rpb2) and the transcription elongation factor 1-alpha gene (ef1-α) regions. Species representation and their relative abundances were similar across all tested barcode regions, despite a lower copy number in protein coding markers. ITS1 and ITS2 required more sophisticated sequence filtering because they produced a high number of chimeric sequences requiring reference-based chimera removal and had a higher number of sequence variants per species. Although clustering of filtered ITS sequences resulted in an average higher number of correctly clustered units at optimal similarity thresholds, these thresholds varied substantially among genera. Best-fitted thresholds of low-copy markers were more consistent across genera but frequently lacked species resolution due to low intraspecific variability. At some thresholds, we observed multiple species lumped together, and at the same time, species split into multiple partial clusters, which should be taken into consideration when assessing the best clustering thresholds and taxonomic identity of clusters. To achieve the best taxonomic resolution and improve species detection, we recommend combining different markers and applying additional reference-based sorting of clusters. The current availability of rpb2 and ef1-α reference sequences in public databases is far from being complete for all fungal groups, but a combined marker approach can be used for group-specific studies that can build reference data for their own purposes. |
 | Vizzini, A; Consiglio, G; Adamčíková, K; Setti, L; Adamčík, S IMA Fungus, 16 , pp. e144994, 2025. @article{Vizzini2025, title = {Pseudobaeosporoideae, a new subfamily within the Tricholomataceae for the genus Pseudobaeospora (Agaricales, Tricholomatineae) based on morphological and molecular inference}, author = {A. Vizzini and G. Consiglio and K. Adamčíková and L. Setti and S. Adamčík}, doi = {DOI: 10.3897/imafungus.16.144994}, year = {2025}, date = {2025-03-13}, journal = {IMA Fungus}, volume = {16}, pages = {e144994}, abstract = {Based on molecular and morphological evidence the new subfamily Pseudobaeosporoideae of the Tricholomataceae is established within the Tricholomatineae for accommodating the unique features of Pseudobaeospora such as gymnocarpic mycenoid/collybioid habit, small-sized spores with thick and dextrinoid wall, and presence of crassobasidia. Twenty-six Pseudobaeospora collections corresponding to eleven species (five types) were newly sequenced. Collections morphologically attributable to P. oligophylla (type of the genus) or to P. pillodii are here sequenced for the first time: accordingly, P. oligophylla is considered as a posterior synonym of P. pillodii. Quélet’s original plate is selected as a lectotype for Collybia pillodii and a French collection as its epitype collection. Pseudobaeospora deceptiva is described as a new species from Italy very close to P. pillodii from which it differs mainly by bigger spores and SSU and LSU rDNA sequences. The presence of P. pyrifera in Italy is documented for the first time and P. mutabilis is reduced to its later synonym. A neotype is established for P. jamonii which is here proved to be an independent species. Finally, a critical review of the characters used for interspecific distinctions in Pseudobaeospora was provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Based on molecular and morphological evidence the new subfamily Pseudobaeosporoideae of the Tricholomataceae is established within the Tricholomatineae for accommodating the unique features of Pseudobaeospora such as gymnocarpic mycenoid/collybioid habit, small-sized spores with thick and dextrinoid wall, and presence of crassobasidia. Twenty-six Pseudobaeospora collections corresponding to eleven species (five types) were newly sequenced. Collections morphologically attributable to P. oligophylla (type of the genus) or to P. pillodii are here sequenced for the first time: accordingly, P. oligophylla is considered as a posterior synonym of P. pillodii. Quélet’s original plate is selected as a lectotype for Collybia pillodii and a French collection as its epitype collection. Pseudobaeospora deceptiva is described as a new species from Italy very close to P. pillodii from which it differs mainly by bigger spores and SSU and LSU rDNA sequences. The presence of P. pyrifera in Italy is documented for the first time and P. mutabilis is reduced to its later synonym. A neotype is established for P. jamonii which is here proved to be an independent species. Finally, a critical review of the characters used for interspecific distinctions in Pseudobaeospora was provided. |
 | Raček, M; Adamčíková, K; Peterková, V; Iľko, I Culturable fungal species in a Verticillium nonalfalfae treated and nontreated Ailanthus altissima stand in Slovakia Journal Article Journal of Plant Pathology, 107 (2), pp. 1171-1182, 2025, ISSN: 2239-7264. @article{Raček2025, title = {Culturable fungal species in a Verticillium nonalfalfae treated and nontreated Ailanthus altissima stand in Slovakia}, author = {M. Raček and K. Adamčíková and V. Peterková and I. Iľko}, doi = {10.1007/s42161-025-01893-3}, issn = {2239-7264}, year = {2025}, date = {2025-03-10}, journal = {Journal of Plant Pathology}, volume = {107}, number = {2}, pages = {1171-1182}, abstract = { Ailanthus altissima Mill. (Swingle), one of the worst invasive tree species in the world, represents an ecological burden on native forested ecosystems. In its management, numerous general removal procedures (including mechanical, chemical and biological) can be applied. Biological is one of the recent control strategies and the fungal strains are the most promising organisms in this field. Culturable fungal species from two wilting A. altissima stands in Slovakia were examined: a stand artificially treated with Verticillium nonalfalfae and a non-treated stand. The aim of this work was to a/ confirm the establishment of V. nonalfalfae in the treated stand, b/ investigate the fungi associated with symptomatic trees in the non-treated stand to determine if V. nonalfalfae spread may have occurred, and c/ consider the biological control potential of other isolated fungi identified. In stands, branches and leaves from wilting A. altissima were collected and cultured on agar. Resulting fungal colonies were sub-cultured for morphological and molecular identification. Eighteen fungal species were identified from 36 branch and 3 leaf samples from symptomatic trees within both stands. Verticillium nonalfalfae was isolated only from the treated stand while V. dahliae was isolated only from the non-treated stand. This confirms the establishment of V. nonalfalfae within the treated stand but does not confirm any additional spread. The isolation of V. dahliae, another known wilt-causing pathogen of A. altissima, from the non-treated stand indicates the natural presence of this pathogen. There have been no previous reports of Verticillium wilt caused by either species on A. altissima in Slovakia. A variety of other culturable fungal species were identified including a few species which were detected for the first time from A. altissima and five Diaporthe species, a genus that includes some known plant pathogens.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ailanthus altissima Mill. (Swingle), one of the worst invasive tree species in the world, represents an ecological burden on native forested ecosystems. In its management, numerous general removal procedures (including mechanical, chemical and biological) can be applied. Biological is one of the recent control strategies and the fungal strains are the most promising organisms in this field. Culturable fungal species from two wilting A. altissima stands in Slovakia were examined: a stand artificially treated with Verticillium nonalfalfae and a non-treated stand. The aim of this work was to a/ confirm the establishment of V. nonalfalfae in the treated stand, b/ investigate the fungi associated with symptomatic trees in the non-treated stand to determine if V. nonalfalfae spread may have occurred, and c/ consider the biological control potential of other isolated fungi identified. In stands, branches and leaves from wilting A. altissima were collected and cultured on agar. Resulting fungal colonies were sub-cultured for morphological and molecular identification. Eighteen fungal species were identified from 36 branch and 3 leaf samples from symptomatic trees within both stands. Verticillium nonalfalfae was isolated only from the treated stand while V. dahliae was isolated only from the non-treated stand. This confirms the establishment of V. nonalfalfae within the treated stand but does not confirm any additional spread. The isolation of V. dahliae, another known wilt-causing pathogen of A. altissima, from the non-treated stand indicates the natural presence of this pathogen. There have been no previous reports of Verticillium wilt caused by either species on A. altissima in Slovakia. A variety of other culturable fungal species were identified including a few species which were detected for the first time from A. altissima and five Diaporthe species, a genus that includes some known plant pathogens. |
 | Lukasová, V; Varšová, S; Žatková, L; Adamčíková, - K; Buchholceová, A; Onderka, M; Milovský, R; Bilčík, D; Mináriková, V Indication of the sensitivity of Pinaceae species growing in Eastern Central Europe to ground-level ozone pollution. Journal Article Environmental Science and Pollution Research, 32 (2), pp. 2638-2655, 2025. @article{Lukasová2025, title = {Indication of the sensitivity of Pinaceae species growing in Eastern Central Europe to ground-level ozone pollution.}, author = {V. Lukasová and S. Varšová and L. Žatková and - K. Adamčíková and A. Buchholceová and M. Onderka and R. Milovský and D. Bilčík and V. Mináriková}, doi = {doi.org/10.1007/s11356-025-35905-7}, year = {2025}, date = {2025-01-14}, journal = {Environmental Science and Pollution Research}, volume = {32}, number = {2}, pages = {2638-2655}, abstract = {of oxidative stress simulated in manipulative experiments. Needles were collected from mature individuals of Pinus mugo, Pinus cembra, Pinus sylvestris, Abies alba, and Picea abies at the foothill (FH) and alpine treeline ecotone (ATE) in the High Tatras (Western Carpathians). The injury index (INX), quantified by the modified electrolyte leakage (EL) method, indicated severe needle damage due to exposure to extremely high levels of O3. Ozonation induced changes in the chemical composition of the needles, which were detected via gas chromatography-mass spectrometry. The oxidative stability (OxS) indicator derived from INXs was used to determine the stomatal O3 flux-based critical level CL(OxS), with the threshold value of OxS at -0.05, corresponding to 5% injury to the needles. Assessment of the phytotoxic ozone dose (POD0) under ambient O3 and field environmental conditions during the 2023 growing season via CL(OxS) revealed that the studied species utilised between 18% (Abies alba FH) and 33% (Pinus mugo ATE) of their O3 tolerance potential. These results support our hypothesis that Pinaceae species growing in the High Tatras, which are part of the Alpine biogeographical region of Eastern Central Europe, are vulnerable to O3 concentrations significantly higher than the typical ambient O3 level in the natural environment.}, keywords = {}, pubstate = {published}, tppubtype = {article} } of oxidative stress simulated in manipulative experiments. Needles were collected from mature individuals of Pinus mugo, Pinus cembra, Pinus sylvestris, Abies alba, and Picea abies at the foothill (FH) and alpine treeline ecotone (ATE) in the High Tatras (Western Carpathians). The injury index (INX), quantified by the modified electrolyte leakage (EL) method, indicated severe needle damage due to exposure to extremely high levels of O3. Ozonation induced changes in the chemical composition of the needles, which were detected via gas chromatography-mass spectrometry. The oxidative stability (OxS) indicator derived from INXs was used to determine the stomatal O3 flux-based critical level CL(OxS), with the threshold value of OxS at -0.05, corresponding to 5% injury to the needles. Assessment of the phytotoxic ozone dose (POD0) under ambient O3 and field environmental conditions during the 2023 growing season via CL(OxS) revealed that the studied species utilised between 18% (Abies alba FH) and 33% (Pinus mugo ATE) of their O3 tolerance potential. These results support our hypothesis that Pinaceae species growing in the High Tatras, which are part of the Alpine biogeographical region of Eastern Central Europe, are vulnerable to O3 concentrations significantly higher than the typical ambient O3 level in the natural environment. |
2024 |
|
 | Pastirčáková, K; Baková, K; Adamčíková, K; Barta, M; Lalík, M; Pavlík, M; Kunca, A; Galko, J; Pastirčák, M Fungi associated with ambrosia beetle Xylosandrus germanus in Slovakia Journal Article Biologia, 79 (8), pp. 2387-2400, 2024, ISSN: 0006-3088. @article{Pastirčáková2024, title = {Fungi associated with ambrosia beetle \textit{Xylosandrus germanus} in Slovakia}, author = {K. Pastirčáková and K. Baková and K. Adamčíková and M. Barta and M. Lalík and M. Pavlík and A. Kunca and J. Galko and M. Pastirčák}, doi = {10.1007/s11756-024-01712-7}, issn = {0006-3088}, year = {2024}, date = {2024-08-21}, journal = {Biologia}, volume = {79}, number = {8}, pages = {2387-2400}, abstract = {The black timber bark beetle (Xylosandrus germanus) is a strongly invasive ambrosia beetle and an important forest pest in Slovakia. This pest is closely associated with symbiotic fungi used as its food source. We investigated the fungi associated with X. germanus adults in Slovakia. In this study, Beauveria bassiana, B. pseudobassiana, Clonostachys rosea, Fusarium oxysporum, Ophiostoma quercus, Phaeoacremonium scolyti, and Talaromyces amestolkiae were isolated and identified by morphological and molecular analyses. The fungus Ophiostoma quercus was most frequently isolated from living beetles, while the entomopathogenic Beauveria bassiana was the most commonly isolated from dead beetles. The morphological descriptions of fungi based on isolates from the surface of X. germanus adults are provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The black timber bark beetle (Xylosandrus germanus) is a strongly invasive ambrosia beetle and an important forest pest in Slovakia. This pest is closely associated with symbiotic fungi used as its food source. We investigated the fungi associated with X. germanus adults in Slovakia. In this study, Beauveria bassiana, B. pseudobassiana, Clonostachys rosea, Fusarium oxysporum, Ophiostoma quercus, Phaeoacremonium scolyti, and Talaromyces amestolkiae were isolated and identified by morphological and molecular analyses. The fungus Ophiostoma quercus was most frequently isolated from living beetles, while the entomopathogenic Beauveria bassiana was the most commonly isolated from dead beetles. The morphological descriptions of fungi based on isolates from the surface of X. germanus adults are provided. |
 | Jančovičová, S; Adamčíková, K; Caboň, M; Graddy, M G; Matheny, P B; Noffsinger, Ch. R; Wheeler, T B; Adamčík, S Taxonomic reintroduction of the holarctic saprotrophic fungus Crepidotus cinnamomeus Journal Article Mycological Progress, 23 , pp. 49, 2024, ISSN: 1617-416X. @article{Jančovičová2024, title = {Taxonomic reintroduction of the holarctic saprotrophic fungus Crepidotus cinnamomeus}, author = {S. Jančovičová and K. Adamčíková and M. Caboň and M. G. Graddy and P. B. Matheny and Ch. R. Noffsinger and T. B. Wheeler and S. Adamčík}, doi = {https://doi.org/10.1007/s11557-024-01991-z}, issn = {1617-416X}, year = {2024}, date = {2024-08-16}, journal = {Mycological Progress}, volume = {23}, pages = {49}, abstract = {Crepidotus is a genus of common saprotrophic fungi well known especially in the Northern Hemisphere, but distribution patterns of individual species are not sufficiently understood. We redefined a taxonomic circumscription of Crepidotus cinnamomeus based on morphological and molecular congruencies between the type material and recent collections. The species is well delimited from other similar and currently accepted species of the genus. Crepidotus cinnamomeus was found to have a broad holarctic distribution with occurrences in North America, Europe and Asia where it grows on twigs and branches of deciduous trees and shrubs in preferably cold humid habitats. Here we present the first multilocus phylogeny of the genus, including portions of the RPB2 gene. Our study highlights the importance of sufficient sampling from broader areas supported by sequence data, which is essential for estimation of species delimitation, distribution and correct name assignment for Crepidotus species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Crepidotus is a genus of common saprotrophic fungi well known especially in the Northern Hemisphere, but distribution patterns of individual species are not sufficiently understood. We redefined a taxonomic circumscription of Crepidotus cinnamomeus based on morphological and molecular congruencies between the type material and recent collections. The species is well delimited from other similar and currently accepted species of the genus. Crepidotus cinnamomeus was found to have a broad holarctic distribution with occurrences in North America, Europe and Asia where it grows on twigs and branches of deciduous trees and shrubs in preferably cold humid habitats. Here we present the first multilocus phylogeny of the genus, including portions of the RPB2 gene. Our study highlights the importance of sufficient sampling from broader areas supported by sequence data, which is essential for estimation of species delimitation, distribution and correct name assignment for Crepidotus species. |
Ondrušková, E; Kobza, M; Jánošíková, Z; McDougal, R; Adamčíková, K Which Cyclaneusma minus morphotypes are responsible for needle cast of Pinus sp. in Slovakia? Journal Article Journal of Plant Diseases and Protection, 131 , pp. 1665–1672, 2024, ISSN: 1861-3829. @article{Ondrušková2024, title = {Which Cyclaneusma minus morphotypes are responsible for needle cast of Pinus sp. in Slovakia?}, author = {E. Ondrušková and M. Kobza and Z. Jánošíková and R. McDougal and K. Adamčíková}, doi = {https://doi.org/10.1007/s41348-024-00924-y}, issn = {1861-3829}, year = {2024}, date = {2024-05-10}, journal = {Journal of Plant Diseases and Protection}, volume = {131}, pages = {1665–1672}, abstract = {Cyclaneusma needle cast (CNC) is a needle disease which caused deterioration of vitality and reduction in the growth of pines. The disease is caused by the ascomycetous fungus Cyclaneusma minus, which has two well-described morphotypes; C. minus simile and C. minus verum. The distribution and host range of C. minus simile and verum was determined from needle samples and isolates collected throughout Slovakia from 2014 to 2020. Samples from 111 localities, 11 pine host species and 245 trees collected in different types of planting were analysed. It was found, that both morphotypes are present, but C. minus verum is predominantly responsible for CNC in urban and forest plantings in Slovakia. C. minus verum was positively detected in more than 88% of collected samples, whereas C. minus simile was only in four samples from three localities. Morphotype-specific primers were sufficiently sensitive even for new pine-host species. The host range of C. minus simile and C. minus verum was enriched worldwide. C. minus verum was observed in nine host species, whereas C. minus simile was identified only in one. Cyclaneusma niveum was also recorded and its presence was confirmed through DNA sequencing.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Cyclaneusma needle cast (CNC) is a needle disease which caused deterioration of vitality and reduction in the growth of pines. The disease is caused by the ascomycetous fungus Cyclaneusma minus, which has two well-described morphotypes; C. minus simile and C. minus verum. The distribution and host range of C. minus simile and verum was determined from needle samples and isolates collected throughout Slovakia from 2014 to 2020. Samples from 111 localities, 11 pine host species and 245 trees collected in different types of planting were analysed. It was found, that both morphotypes are present, but C. minus verum is predominantly responsible for CNC in urban and forest plantings in Slovakia. C. minus verum was positively detected in more than 88% of collected samples, whereas C. minus simile was only in four samples from three localities. Morphotype-specific primers were sufficiently sensitive even for new pine-host species. The host range of C. minus simile and C. minus verum was enriched worldwide. C. minus verum was observed in nine host species, whereas C. minus simile was identified only in one. Cyclaneusma niveum was also recorded and its presence was confirmed through DNA sequencing. | |
 | Jánošíková, Z; Kobza, M; Ondrušková, E; Ostrovský, R; Pažitný, J; Adamčíková, K Virulence of Dothistroma septosporum and D. pini on Pinus nigra and P. mugo under conditions of natural infection Journal Article European Journal of Plant Pathology, 168 (2), pp. 775-785, 2024. @article{Jánošíková2024, title = {Virulence of Dothistroma septosporum and D. pini on Pinus nigra and P. mugo under conditions of natural infection}, author = {Z. Jánošíková and M. Kobza and E. Ondrušková and R. Ostrovský and J. Pažitný and K. Adamčíková}, url = {http://ife.sk/wp-content/uploads/2020/09/Fig_edited.jpghttp://ife.sk/wp-content/uploads/2020/09/final-1-2.jpg}, doi = {https://doi.org/10.1007/s10658-023-02799-5}, year = {2024}, date = {2024-04-04}, journal = {European Journal of Plant Pathology}, volume = {168}, number = {2}, pages = {775-785}, abstract = {Dothistroma needle blight (DNB) is a severe needle disease of pines worldwide, caused by two closely related species, Dothistroma septosporum and D. pini. The two fungal species are similar not only in their morphological characteristics, but also cause very similar symptoms in their hosts, and have a similar ecology. The aim of this study was to compare the virulence of the two Dothistroma species in natural infection experiments on 2-year-old seedlings of two DNB susceptible pine species, Pinus nigra and P. mugo, in two seedling stands for each pathogen species. The virulence of the pathogens and presence of symptoms (symptomatic needles, red bands and acervuli) were assessed after 2 years of exposure to inoculum. The incidence of seedlings with DNB symptoms was 65% and 76% for P. nigra and P. mugo, respectively. No difference was found between D. septosporum and D. pini in any of the three DNB symptoms evaluated on seedlings of P. mugo. However, symptoms of disease differed between the two Dothistroma species on P. nigra. Variables, which reflect the intensity of disease development, the number of red bands and acervuli per needle, showed a difference in virulence between D. septosporum and D. pini, but only in the case of the host species P. nigra. The results suggest that the virulence of the two Dothistroma species could be affected by host pine species and that there are differences in susceptibility of individual pine species to D. septosporum and D. pini. Further factors could affect the virulence of these pathogens, including isolate origin, climatic or environmental factors.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a severe needle disease of pines worldwide, caused by two closely related species, Dothistroma septosporum and D. pini. The two fungal species are similar not only in their morphological characteristics, but also cause very similar symptoms in their hosts, and have a similar ecology. The aim of this study was to compare the virulence of the two Dothistroma species in natural infection experiments on 2-year-old seedlings of two DNB susceptible pine species, Pinus nigra and P. mugo, in two seedling stands for each pathogen species. The virulence of the pathogens and presence of symptoms (symptomatic needles, red bands and acervuli) were assessed after 2 years of exposure to inoculum. The incidence of seedlings with DNB symptoms was 65% and 76% for P. nigra and P. mugo, respectively. No difference was found between D. septosporum and D. pini in any of the three DNB symptoms evaluated on seedlings of P. mugo. However, symptoms of disease differed between the two Dothistroma species on P. nigra. Variables, which reflect the intensity of disease development, the number of red bands and acervuli per needle, showed a difference in virulence between D. septosporum and D. pini, but only in the case of the host species P. nigra. The results suggest that the virulence of the two Dothistroma species could be affected by host pine species and that there are differences in susceptibility of individual pine species to D. septosporum and D. pini. Further factors could affect the virulence of these pathogens, including isolate origin, climatic or environmental factors. |
 | Noffsinger, Ch. R; Adamčíková, K; Eberhardt, U; Caboň, M; Bazzicalup, A; Buyck, B; Kaufmann, H; Weholt, Ø; Looney, B P; Matheny, P B; Berbee, M L; Tausan, D; Adamčík, S Three new species in Russula subsection Xerampelinae supported by genealogical and phenotypic coherence Journal Article Mycologia, 116 (2), pp. 322-349, 2024. @article{Noffsinger2024, title = {Three new species in Russula subsection Xerampelinae supported by genealogical and phenotypic coherence}, author = {Ch. R. Noffsinger and K. Adamčíková and U. Eberhardt and M. Caboň and A. Bazzicalup and B. Buyck and H. Kaufmann and Ø. Weholt and B. P. Looney and P. B. Matheny and M. L. Berbee and D. Tausan and S. Adamčík}, doi = {10.1080/00275514.2023.2295957}, year = {2024}, date = {2024-02-16}, journal = {Mycologia}, volume = {116}, number = {2}, pages = {322-349}, abstract = {Xerampelinae is a subsection composed of species of ectomycorrhizal fungi belonging to the hyperdiverse and cosmopolitan genus Russula (Russulales). Species of Xerampelinae are recognized by their fishy or shrimp odor, browning context, and a green reaction to iron sulfate. However, species delimitation has traditionally relied on morphology and analysis of limited molecular data. Prior taxonomic work in Xerampelinae has led to the description of as many as 59 taxa in Europe and 19 in North America. Here we provide the first multilocus phylogeny of European and North American members based on two nrDNA loci and two protein-coding genes. The resulting phylogeny supports the recognition of 17 species-rank Xerampelinae clades; however, higher species richness (~23) is suggested by a more inclusive nuclear rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS barcode) analysis. Phylogenetic and morphological analyses support three new species with restricted geographic distributions: R. lapponica, R. neopascua, and R. olympiana. We confirm that the European species R. subrubens is present in North America and the North American species R. serissima previously known as R. favrei) is present in Europe. Most other Xerampelinae appear restricted to either North America or Eurasia, which indicates a high degree of regional endemism; this includes R. xerampelina, a name widely applied to North American taxa, but a species restricted to Eurasia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Xerampelinae is a subsection composed of species of ectomycorrhizal fungi belonging to the hyperdiverse and cosmopolitan genus Russula (Russulales). Species of Xerampelinae are recognized by their fishy or shrimp odor, browning context, and a green reaction to iron sulfate. However, species delimitation has traditionally relied on morphology and analysis of limited molecular data. Prior taxonomic work in Xerampelinae has led to the description of as many as 59 taxa in Europe and 19 in North America. Here we provide the first multilocus phylogeny of European and North American members based on two nrDNA loci and two protein-coding genes. The resulting phylogeny supports the recognition of 17 species-rank Xerampelinae clades; however, higher species richness (~23) is suggested by a more inclusive nuclear rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS barcode) analysis. Phylogenetic and morphological analyses support three new species with restricted geographic distributions: R. lapponica, R. neopascua, and R. olympiana. We confirm that the European species R. subrubens is present in North America and the North American species R. serissima previously known as R. favrei) is present in Europe. Most other Xerampelinae appear restricted to either North America or Eurasia, which indicates a high degree of regional endemism; this includes R. xerampelina, a name widely applied to North American taxa, but a species restricted to Eurasia. |
2023 |
|
 | Jánošíková, Z; Ondrušková, E; Adamčíková, K Artificial inoculation and susceptibility of Pinus armandii to Dothistroma septosporum Journal Article Forest Pathology, 53 (5), pp. e12826, 2023, ISSN: 1439-0329. @article{Jánošíková2023b, title = {Artificial inoculation and susceptibility of Pinus armandii to Dothistroma septosporum}, author = {Z. Jánošíková and E. Ondrušková and K. Adamčíková}, doi = {DOI: 10.1111/efp.12826 }, issn = {1439-0329}, year = {2023}, date = {2023-08-18}, journal = {Forest Pathology}, volume = {53}, number = {5}, pages = {e12826}, abstract = {Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a serious needle disease of conifers that primarily affects pine species (Pinus spp.). Dothistroma septosporum is one of the DNB pathogens that has a diverse range of host species excluding Pinus armandii. In 15 inoculated P. armandii seedlings, D. septosporum acervuli were observed in 43 infected needles of ten seedlings with a mean disease severity of 1.11% at 25 weeks after inoculations, demonstrating the potential of D. septosporum to cause symptoms on the needles of P. armandii via artificial inoculation. The disease severity of P. armandii was similar to the positive control, Pinus nigra (median 0.75 for P. armandii to 0.70 for P. nigra), thus, P. armandii acts under artificial conditions as a susceptible host species. |
 | Adamčíková, K; Pastirčáková, K; Jánošíková, Z; Ostrovský, R; Pastirčák, M; Pažitný, J; Kobza, M; Adamčík, S; Kádasi-Horáková, M; Ondrušková, E New regional records of Dothistroma needle blight pathogens from Slovakia: distribution, hosts and pathogens characterization Journal Article Annals of Forest Research, 66 (1), pp. 99-111, 2023, ISSN: 1844-8135. @article{Adamčíková2023, title = {New regional records of Dothistroma needle blight pathogens from Slovakia: distribution, hosts and pathogens characterization}, author = {K. Adamčíková and K. Pastirčáková and Z. Jánošíková and R. Ostrovský and M. Pastirčák and J. Pažitný and M. Kobza and S. Adamčík and M. Kádasi-Horáková and E. Ondrušková}, doi = {https://doi.org/10.15287/afr.2023.2427}, issn = {1844-8135}, year = {2023}, date = {2023-07-25}, journal = {Annals of Forest Research}, volume = {66}, number = {1}, pages = {99-111}, abstract = {Dothistroma needle blight is one of the most severe needle diseases of pines caused by two closely related species, Dothistroma septosporum and D. pini. The further spread and distribution of this disease was investigated in Slovakia as well as new hosts and stand types were identified at regional level. Dothistroma septosporum was recorded in a natural stand at higher altitude on Pinus cembra in the High Tatras and the P. uncinata records are new host reports for Slovakia for this pathogen. Moreover, for D. pini, P. cembra as a new host at the country level was recorded and P. armandii was identified as new host species worldwide. Mating types for all collected samples and ITS haplotypes for D. pini isolates were determined. For D. pini, five localities with the presence of both mating types and three ITS haplotypes (Dp_HAP.1, Dp_HAP.2 and Dp_HAP.4) were reported. Samples where both mating types of the pathogens were identified, were selected for the microscopic examination of fruiting bodies aimed to detect sexual reproductive organs. In all inspected needle samples of D. pini, only conidiomata with typical hyaline cylindrical conidia were identified. The sexual state of D. septosporum was recorded in one sample of P. nigra needles.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight is one of the most severe needle diseases of pines caused by two closely related species, Dothistroma septosporum and D. pini. The further spread and distribution of this disease was investigated in Slovakia as well as new hosts and stand types were identified at regional level. Dothistroma septosporum was recorded in a natural stand at higher altitude on Pinus cembra in the High Tatras and the P. uncinata records are new host reports for Slovakia for this pathogen. Moreover, for D. pini, P. cembra as a new host at the country level was recorded and P. armandii was identified as new host species worldwide. Mating types for all collected samples and ITS haplotypes for D. pini isolates were determined. For D. pini, five localities with the presence of both mating types and three ITS haplotypes (Dp_HAP.1, Dp_HAP.2 and Dp_HAP.4) were reported. Samples where both mating types of the pathogens were identified, were selected for the microscopic examination of fruiting bodies aimed to detect sexual reproductive organs. In all inspected needle samples of D. pini, only conidiomata with typical hyaline cylindrical conidia were identified. The sexual state of D. septosporum was recorded in one sample of P. nigra needles. |
 | Kádasi-Horáková, M; Barta, M; Adamčíková, K; Ostrovský, R; Pastirčáková, K Hymenoscyphus fraxineus on Fraxinus excelsior in Slovakia: distribution and mating types Journal Article Biologia, 78 (5), pp. 1219-1230, 2023, ISSN: 0006-3088. @article{Kádasi-Horáková2023, title = {\textit{Hymenoscyphus fraxineus} on \textit{Fraxinus excelsior} in Slovakia: distribution and mating types}, author = {M. Kádasi-Horáková and M. Barta and K. Adamčíková and R. Ostrovský and K. Pastirčáková}, doi = {10.1007/s11756-022-01023-9}, issn = {0006-3088}, year = {2023}, date = {2023-05-10}, journal = {Biologia}, volume = {78}, number = {5}, pages = {1219-1230}, abstract = {Hymenoscyphus fraxineus causes ash dieback in Europe and threatens the future existence of Fraxinus excelsior in large parts of its natural distribution range. In this study, we report the first documented distribution of the pathogen on the most common ash species F. excelsior in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region of rDNA. Analysis of the mating type genes of H. fraxineus isolates revealed the presence of both mating types in Slovakia. Hymenoscyphus fraxineus-positive trees were recorded in 70 localities with different types of management in different parts of the country, mainly in forest stands. The results indicate the widespread distribution of H. fraxineus across the entire country, wherever the host is present.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Hymenoscyphus fraxineus causes ash dieback in Europe and threatens the future existence of Fraxinus excelsior in large parts of its natural distribution range. In this study, we report the first documented distribution of the pathogen on the most common ash species F. excelsior in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region of rDNA. Analysis of the mating type genes of H. fraxineus isolates revealed the presence of both mating types in Slovakia. Hymenoscyphus fraxineus-positive trees were recorded in 70 localities with different types of management in different parts of the country, mainly in forest stands. The results indicate the widespread distribution of H. fraxineus across the entire country, wherever the host is present. |
 | Jánošíková, R; Adamčíková, K Development and changes in pathogens population causing Dothistroma needle blight in Pinus nigra plantation in Strážovské vrchy Mts Journal Article Biologia, 78 (5), pp. 1231-1237, 2023, ISSN: 1336-9563. @article{Jánošíková2023, title = {Development and changes in pathogens population causing Dothistroma needle blight in Pinus nigra plantation in Strážovské vrchy Mts}, author = {R. Jánošíková and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s11756-022-01135-2}, doi = {doi.org/10.1007/s11756-022-01135-2}, issn = {1336-9563}, year = {2023}, date = {2023-05-10}, journal = {Biologia}, volume = {78}, number = {5}, pages = {1231-1237}, abstract = {Dothistroma septosporum and Dothistroma pini are two species of ascomycetous fungi of the genus Dothistroma, which cause needle disease worldwide known as Dothistroma needle blight (DNB). DNB attacks needles mostly from the Pinus species and causes signifcant defoliation on their hosts. Both Dothistroma species produce identical symptoms, the only unambiguous method of distinguishing are molecular identifcation methods. PCR techniques using species and mating-type specifc primers were used to compare the development of the population structures of both fungal pathogens after 12 years. The basis for this comparative analysis was the biological material we collected from the P. nigra plantation in 2018 and the collection of 24 isolates from the same research area collected in 2006. From the whole number of 107 established cultures, we obtained 56 isolates, while the presence of both Dothistroma species was confrmed. However, 12 years ago only D. septosporum occurred at the research area. For the species, D. septosporum, we identifed both mating types with slight dominance of MAT1-2, but still not deviated from a 1:1 ratio (MAT1-1: MAT1-2=26:33), similar results were obtained within samples 12 years before, suggesting sexual reproduction occurs in the population. Whereas single isolate for the species D. pini was identifed as the mating type MAT1-1.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma septosporum and Dothistroma pini are two species of ascomycetous fungi of the genus Dothistroma, which cause needle disease worldwide known as Dothistroma needle blight (DNB). DNB attacks needles mostly from the Pinus species and causes signifcant defoliation on their hosts. Both Dothistroma species produce identical symptoms, the only unambiguous method of distinguishing are molecular identifcation methods. PCR techniques using species and mating-type specifc primers were used to compare the development of the population structures of both fungal pathogens after 12 years. The basis for this comparative analysis was the biological material we collected from the P. nigra plantation in 2018 and the collection of 24 isolates from the same research area collected in 2006. From the whole number of 107 established cultures, we obtained 56 isolates, while the presence of both Dothistroma species was confrmed. However, 12 years ago only D. septosporum occurred at the research area. For the species, D. septosporum, we identifed both mating types with slight dominance of MAT1-2, but still not deviated from a 1:1 ratio (MAT1-1: MAT1-2=26:33), similar results were obtained within samples 12 years before, suggesting sexual reproduction occurs in the population. Whereas single isolate for the species D. pini was identifed as the mating type MAT1-1. |
 | Ondrušková, E; Adamčík, S; Kobza, M; Jánošíková, Z; Ostrovský, R; Pastirčáková, K; Caboň, M; Adamčíková, K Checking the balance between pathogenic and mutualistic pine needle fungi of the genus Lophodermium in forested and urban areas of Slovakia Journal Article Scandinavian Journal of Forest Research, 38 (1-2), pp. 39-48, 2023, ISSN: 1651-1891. @article{Ondrušková2023, title = {Checking the balance between pathogenic and mutualistic pine needle fungi of the genus Lophodermium in forested and urban areas of Slovakia}, author = {E. Ondrušková and S. Adamčík and M. Kobza and Z. Jánošíková and R. Ostrovský and K. Pastirčáková and M. Caboň and K. Adamčíková }, doi = { DOI: 10.1080/02827581.2023.2191004}, issn = {1651-1891}, year = {2023}, date = {2023-03-27}, journal = {Scandinavian Journal of Forest Research}, volume = {38}, number = {1-2}, pages = {39-48}, abstract = {Lophodermium species are well known to be among dominant endophytic fungi colonising pine needles. Occurrence of two species with different life strategies on different Pinus trees across Slovakia was detected by PCR using species specific primers. In general, commensalistic species L. pinastri was more frequent than parasitic L. seditiosum. There were no significant differences observed between urban and natural environments, but natural environments have more frequent colonisation by L. pinastri and less frequent were trees negatively tested to Lophodermium colonisation. Among the most frequently sampled trees were non-native P. nigra and native P. sylvestris. Significant difference in Lophodermium incidence was detected between these two species in natural environment, with increased frequency of L. pinastri on native Scots pine. In addition to one clade of L. pinastri, culture based species identification confirmed presence of L. corconticum and an undescribed clade of L. seditiosum that is not sensitive to used PCR primers for the species detection.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Lophodermium species are well known to be among dominant endophytic fungi colonising pine needles. Occurrence of two species with different life strategies on different Pinus trees across Slovakia was detected by PCR using species specific primers. In general, commensalistic species L. pinastri was more frequent than parasitic L. seditiosum. There were no significant differences observed between urban and natural environments, but natural environments have more frequent colonisation by L. pinastri and less frequent were trees negatively tested to Lophodermium colonisation. Among the most frequently sampled trees were non-native P. nigra and native P. sylvestris. Significant difference in Lophodermium incidence was detected between these two species in natural environment, with increased frequency of L. pinastri on native Scots pine. In addition to one clade of L. pinastri, culture based species identification confirmed presence of L. corconticum and an undescribed clade of L. seditiosum that is not sensitive to used PCR primers for the species detection. |
 | Tubby, K; Adamčíková, K; Adamson, K; Akiba, M; Barnes, I; Boroń, P; Bragança, H; Bulgakov, T; Burgdorf, N; Capretti, P; Cech, T; Cleary, M; Davydenko, K; Drenkhan, R; Elvira-Recuenco, M; Enderle, R; Gardner, J; Georgieva, M; Ghelardini, L; Husson, C; Iturritxa, E; Markovskaja, S; Mesanza, N; Ogris, N; Oskay, F; Piškur, B; Queloz, V; Raitelaityte, K; Raposo, R; Soukainen, M; Strasser, L; Vahalík, P; Vester, M; Mullett, M The increasing threat to European forests from the invasive foliar pine pathogen, Lecanosticta acicola Journal Article Forets Ecology and Management, 536 , pp. 120847, 2023. @article{Tubby2023, title = {The increasing threat to European forests from the invasive foliar pine pathogen, Lecanosticta acicola}, author = {K. Tubby and K. Adamčíková and K. Adamson and M. Akiba and I. Barnes and P. Boroń and H. Bragança and T. Bulgakov and N. Burgdorf and P. Capretti and T. Cech and M. Cleary and K. Davydenko and R. Drenkhan and M. Elvira-Recuenco and R. Enderle and J. Gardner and M. Georgieva and L. Ghelardini and C. Husson and E. Iturritxa and S. Markovskaja and N. Mesanza and N. Ogris and F. Oskay and B. Piškur and V. Queloz and K. Raitelaityte and R. Raposo and M. Soukainen and L. Strasser and P. Vahalík and M. Vester and M. Mullett}, doi = {doi.org/10.1016/j.foreco.2023.120847 }, year = {2023}, date = {2023-03-23}, journal = {Forets Ecology and Management}, volume = {536}, pages = { 120847}, abstract = {European forests are threatened by increasing numbers of invasive pests and pathogens. Over the past century, Lecanosticta acicola, a foliar pathogen predominantly of Pinus spp., has expanded its range globally, and is increasing in impact. Lecanosticta acicola causes brown spot needle blight, resulting in premature defoliation, reduced growth, and mortality in some hosts. Originating from southern regions of North American, it devastated forests in the USA’s southern states in the early twentieth century, and in 1942 was discovered in Spain. Derived from Euphresco project Brownspotrisk,’ this study aimed to establish the current distribution of Lecanosticta species, and assess the risks of L. acicola to European forests. Pathogen reports from the literature, and new/ unpublished survey data were combined into an open-access geo-database (http://www.portaloff orestpathology.com), and used to visualise the pathogen’s range, infer its climatic tolerance, and update its host range. Lecanosticta species have now been recorded in 44 countries, mostly in the northern hemisphere. The type species, L. acicola, has increased its range in recent years, and is present in 24 out of the 26 European countries where data were available. Other species of Lecanosticta are largely restricted to Mexico and Central America, and recently Colombia. The geo-database records demonstrate that L. acicola tolerates a wide range of climates across the northern hemisphere, and indicate its potential to colonise Pinus spp. forests across large swathes of the Europe. Preliminary analyses suggest L. acicola could affect 62% of global Pinus species area by the end of this century, under climate change predictions. Although its host range appears slightly narrower than the similar Dothistroma species, Lecanosticta species were recorded on 70 host taxa, mostly Pinus spp., but including, Cedrus and Picea spp. Twenty-three, including species of critical ecological, environmental and economic significance in Europe, are highly susceptible to L. acicola, suffering heavy defoliation and sometimes mortality. Variation in apparent susceptibility between reports could reflect variation between regions in the hosts’ genetic make-up, but could also reflect the significant variation in L. acicola populations and lineages found across Europe. This study served to highlight significant gaps in our understanding of the pathogen’s behaviour. Lecanosticta acicola has recently been downgraded from an A1 quarantine pest to a regulated non quarantine pathogen, and is now widely distributed across Europe. With a need to consider disease management, this study also explored global BSNB strategies, and used Case Studies to summarise the tactics employed to date in Europe. }, keywords = {}, pubstate = {published}, tppubtype = {article} } European forests are threatened by increasing numbers of invasive pests and pathogens. Over the past century, Lecanosticta acicola, a foliar pathogen predominantly of Pinus spp., has expanded its range globally, and is increasing in impact. Lecanosticta acicola causes brown spot needle blight, resulting in premature defoliation, reduced growth, and mortality in some hosts. Originating from southern regions of North American, it devastated forests in the USA’s southern states in the early twentieth century, and in 1942 was discovered in Spain. Derived from Euphresco project Brownspotrisk,’ this study aimed to establish the current distribution of Lecanosticta species, and assess the risks of L. acicola to European forests. Pathogen reports from the literature, and new/ unpublished survey data were combined into an open-access geo-database (http://www.portaloff orestpathology.com), and used to visualise the pathogen’s range, infer its climatic tolerance, and update its host range. Lecanosticta species have now been recorded in 44 countries, mostly in the northern hemisphere. The type species, L. acicola, has increased its range in recent years, and is present in 24 out of the 26 European countries where data were available. Other species of Lecanosticta are largely restricted to Mexico and Central America, and recently Colombia. The geo-database records demonstrate that L. acicola tolerates a wide range of climates across the northern hemisphere, and indicate its potential to colonise Pinus spp. forests across large swathes of the Europe. Preliminary analyses suggest L. acicola could affect 62% of global Pinus species area by the end of this century, under climate change predictions. Although its host range appears slightly narrower than the similar Dothistroma species, Lecanosticta species were recorded on 70 host taxa, mostly Pinus spp., but including, Cedrus and Picea spp. Twenty-three, including species of critical ecological, environmental and economic significance in Europe, are highly susceptible to L. acicola, suffering heavy defoliation and sometimes mortality. Variation in apparent susceptibility between reports could reflect variation between regions in the hosts’ genetic make-up, but could also reflect the significant variation in L. acicola populations and lineages found across Europe. This study served to highlight significant gaps in our understanding of the pathogen’s behaviour. Lecanosticta acicola has recently been downgraded from an A1 quarantine pest to a regulated non quarantine pathogen, and is now widely distributed across Europe. With a need to consider disease management, this study also explored global BSNB strategies, and used Case Studies to summarise the tactics employed to date in Europe. |
 | van der Nest, A; Wingfield, M J; Sadiković, D; Mullett, M S; Marçais, B; Queloz, V; Adamčíková, K; Davydenko, K; Barnes, I Population structure and diversity of the needle pathogen Dothistroma pini suggests human-mediated movement in Europe Journal Article Frontiers in Genetics, 14 , pp. 1103331, 2023, ISSN: 1338-7014. @article{Bičárová2023b, title = {Population structure and diversity of the needle pathogen Dothistroma pini suggests human-mediated movement in Europe}, author = {A. van der Nest and M. J. Wingfield and D. Sadiković and M. S. Mullett and B. Marçais and V. Queloz and K. Adamčíková and K. Davydenko and I. Barnes}, doi = {doi: 10.3389/fgene.2023.1103331}, issn = {1338-7014}, year = {2023}, date = {2023-02-16}, journal = {Frontiers in Genetics}, volume = {14}, pages = {1103331}, abstract = {Dothistroma needle blight (DNB) is an important disease of Pinus species that can be caused by one of two distinct but closely related pathogens; Dothistroma septosporum and Dothistroma pini. Dothistroma septosporum has a wide geographic distribution and is relatively well-known. In contrast, D. pini is known only from the United States and Europe, and there is a distinct lack of knowledge regarding its population structure and genetic diversity. The recent development of 16 microsatellite markers for D. pini provided an opportunity to investigate the diversity, structure, and mode of reproduction for populations collected over a period of 12 years, on eight different hosts in Europe. In total, 345 isolates from Belgium, the Czech Republic, France, Hungary, Romania, Western Russia, Serbia, Slovakia, Slovenia, Spain, Switzerland, and Ukraine were screened using microsatellite and species-specific mating type markers. A total of 109 unique multilocus haplotypes were identified and structure analyses suggested that the populations are influenced by location rather than host species. Populations from France and Spain displayed the highest levels of genetic diversity followed by the population in Ukraine. Both mating types were detected in most countries, with the exception of Hungary, Russia and Slovenia. Evidence for sexual recombination was supported only in the population from Spain. The observed population structure and several shared haplotypes between non-bordering countries provides good evidence that the movement of D. pini in Europe has been strongly influenced by human activity in Europe.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is an important disease of Pinus species that can be caused by one of two distinct but closely related pathogens; Dothistroma septosporum and Dothistroma pini. Dothistroma septosporum has a wide geographic distribution and is relatively well-known. In contrast, D. pini is known only from the United States and Europe, and there is a distinct lack of knowledge regarding its population structure and genetic diversity. The recent development of 16 microsatellite markers for D. pini provided an opportunity to investigate the diversity, structure, and mode of reproduction for populations collected over a period of 12 years, on eight different hosts in Europe. In total, 345 isolates from Belgium, the Czech Republic, France, Hungary, Romania, Western Russia, Serbia, Slovakia, Slovenia, Spain, Switzerland, and Ukraine were screened using microsatellite and species-specific mating type markers. A total of 109 unique multilocus haplotypes were identified and structure analyses suggested that the populations are influenced by location rather than host species. Populations from France and Spain displayed the highest levels of genetic diversity followed by the population in Ukraine. Both mating types were detected in most countries, with the exception of Hungary, Russia and Slovenia. Evidence for sexual recombination was supported only in the population from Spain. The observed population structure and several shared haplotypes between non-bordering countries provides good evidence that the movement of D. pini in Europe has been strongly influenced by human activity in Europe. |
 | Bičárová, Svetlana; Lukasová, Veronika; Adamčíková, Katarína; Žatková, Lucia; Milovský, Rastislav; Shashikumar, Anumol; Pažitný, Jozef; Buchholcerová, Anna; Bilčík, Dušan Modified electrolyte leakage method for testing the oxidative stability of Pinus mugo Turra under ozone-induced stress Journal Article Folia Oecologica, 50 (1), pp. 1 - 15, 2023. @article{Bičárová2023, title = {Modified electrolyte leakage method for testing the oxidative stability of \textit{Pinus mugo} Turra under ozone-induced stress}, author = {Svetlana Bičárová and Veronika Lukasová and Katarína Adamčíková and Lucia Žatková and Rastislav Milovský and Anumol Shashikumar and Jozef Pažitný and Anna Buchholcerová and Dušan Bilčík}, url = {http://ife.sk/wp-content/uploads/2020/09/foecol-2023-0001.pdf}, doi = {10.2478/foecol-2023-0001}, year = {2023}, date = {2023-01-27}, journal = {Folia Oecologica}, volume = {50}, number = {1}, pages = {1 - 15}, abstract = {Electrolyte leakage (EL) is the method commonly used to test the cell membrane integrity of plants under stress conditions. The cells of the leaf may be damaged by ozone (O3) entering the intercellular space as an oxidative stress agent. The modified EL method was used to test the oxidative stability (OxS) of plant tissue against O3-induced oxidative stress. The modification includes simulation of the artificial oxidative stress by additional ozonation of plant samples in the laboratory chamber. This modified EL method was applied to Pinus mugo Turra needle samples collected in the subalpine zone of the High Tatra Mts (Western Carpathians), in the years 2019 and 2020. Changes in the chemical composition of samples after artificial ozonation were traced by gas chromatography/mass spectrometry (GC/MS) analysis. In addition, O3 uptake through open stomata was estimated by calculation of the modelled ozone dose (MO3D). We also conducted an inspection of visible injury (VIN) on the needle surface focused on the occurrence of O3-induced symptoms and biotic harmful agents. Regarding OxS results as well as VIN indices, P. mugo needles showed relatively low sensitivity to oxidative stress induced by O3. Therefore MO3D in a range between 14 and 16 mmol m−2 can be considered as O3 dose with minor phytotoxic effect on P. mugo growing in the mountains of central-eastern Europe.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Electrolyte leakage (EL) is the method commonly used to test the cell membrane integrity of plants under stress conditions. The cells of the leaf may be damaged by ozone (O3) entering the intercellular space as an oxidative stress agent. The modified EL method was used to test the oxidative stability (OxS) of plant tissue against O3-induced oxidative stress. The modification includes simulation of the artificial oxidative stress by additional ozonation of plant samples in the laboratory chamber. This modified EL method was applied to Pinus mugo Turra needle samples collected in the subalpine zone of the High Tatra Mts (Western Carpathians), in the years 2019 and 2020. Changes in the chemical composition of samples after artificial ozonation were traced by gas chromatography/mass spectrometry (GC/MS) analysis. In addition, O3 uptake through open stomata was estimated by calculation of the modelled ozone dose (MO3D). We also conducted an inspection of visible injury (VIN) on the needle surface focused on the occurrence of O3-induced symptoms and biotic harmful agents. Regarding OxS results as well as VIN indices, P. mugo needles showed relatively low sensitivity to oxidative stress induced by O3. Therefore MO3D in a range between 14 and 16 mmol m−2 can be considered as O3 dose with minor phytotoxic effect on P. mugo growing in the mountains of central-eastern Europe. |
2022 |
|
 | Laas, M; Adamson, K; Barnes, I; Janoušek, J; Mullett, M S; Adamčíková, K; Akiba, M; Beenken, L; Braganca, H; Bulgakov, T S; Capretti, P; Cech, T; Cleary, M; Enderle, R; Ghelardini, L; Jankovský, L; Markovskaja, S; Matsiakh, I; Meyer, J B; Oskay, F; Piškur, B; Raitelaitytė, K; Sadiković, D; Drenkhan, R Diversity, migration routes, and worldwide population genetic structure of Lecanosticta acicola, the causal agent of brown spot needle blight Journal Article Molecular Plant Pathology, 23 (11), pp. 1620-1639, 2022, ISSN: 1364-3703. @article{Laas2022, title = {Diversity, migration routes, and worldwide population genetic structure of Lecanosticta acicola, the causal agent of brown spot needle blight}, author = {M. Laas and K. Adamson and I. Barnes and J. Janoušek and M.S. Mullett and K. Adamčíková and M. Akiba and L. Beenken and H. Braganca and T.S. Bulgakov and P. Capretti and T. Cech and M. Cleary and R. Enderle and L. Ghelardini and L. Jankovský and S. Markovskaja and I. Matsiakh and J.B. Meyer and F. Oskay and B. Piškur and K. Raitelaitytė and D. Sadiković and R. Drenkhan}, url = {http://ife.sk/wp-content/uploads/2020/09/Bez-názvu.tiff}, doi = {DOI: 10.1111/mpp.13257}, issn = {1364-3703}, year = {2022}, date = {2022-10-14}, journal = {Molecular Plant Pathology}, volume = {23}, number = {11}, pages = {1620-1639}, abstract = {Lecanosticta acicola is a pine needle pathogen causing brown spot needle blight that results in premature needle shedding with considerable damage described in North America, Europe, and Asia. Microsatellite and mating type markers were used to study the population genetics, migration history, and reproduction mode of the pathogen, based on a collection of 650 isolates from 27 countries and 26 hosts across the range of L. acicola. The presence of L. acicola in Georgia was confirmed in this study. Migration analyses indicate there have been several introduction events from North America into Europe. However, some of the source populations still appear to remain unknown. The populations in Croatia and western Asia appear to originate from genetically similar populations in North America. Intercontinental movement of the pathogen was reflected in an identical haplotype occurring on two continents, in North America (Canada) and Europe (Germany). Several shared haplotypes between European populations further suggests more local pathogen movement between countries. Moreover, migration analyses indicate that the populations in northern Europe originate from more established populations in central Europe. Overall, the highest genetic diversity was observed in south-eastern USA. In Europe, the highest diversity was observed in France, where the presence of both known pathogen lineages was recorded. Less than half of the observed populations contained mating types in equal proportions. Although there is evidence of some sexual reproduction taking place, the pathogen spreads predominantly asexually and through anthropogenic activity.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Lecanosticta acicola is a pine needle pathogen causing brown spot needle blight that results in premature needle shedding with considerable damage described in North America, Europe, and Asia. Microsatellite and mating type markers were used to study the population genetics, migration history, and reproduction mode of the pathogen, based on a collection of 650 isolates from 27 countries and 26 hosts across the range of L. acicola. The presence of L. acicola in Georgia was confirmed in this study. Migration analyses indicate there have been several introduction events from North America into Europe. However, some of the source populations still appear to remain unknown. The populations in Croatia and western Asia appear to originate from genetically similar populations in North America. Intercontinental movement of the pathogen was reflected in an identical haplotype occurring on two continents, in North America (Canada) and Europe (Germany). Several shared haplotypes between European populations further suggests more local pathogen movement between countries. Moreover, migration analyses indicate that the populations in northern Europe originate from more established populations in central Europe. Overall, the highest genetic diversity was observed in south-eastern USA. In Europe, the highest diversity was observed in France, where the presence of both known pathogen lineages was recorded. Less than half of the observed populations contained mating types in equal proportions. Although there is evidence of some sexual reproduction taking place, the pathogen spreads predominantly asexually and through anthropogenic activity. |
 | Lukasová, V; Bičárová, S; Buchholcerová, A; Adamčíková, K Low sensitivity of Pinus mugo to surface ozone pollution in the subalpine zone of continental Europe Journal Article International Journal of Biometeorology, 66 (11), pp. 2311–2324, 2022, ISSN: 0020-7128. @article{Lukasová2022, title = {Low sensitivity of Pinus mugo to surface ozone pollution in the subalpine zone of continental Europe}, author = {V. Lukasová and S. Bičárová and A. Buchholcerová and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s00484-022-02359-2}, doi = {10.1007/s00484-022-02359-2}, issn = {0020-7128}, year = {2022}, date = {2022-09-15}, journal = {International Journal of Biometeorology}, volume = {66}, number = {11}, pages = {2311–2324}, abstract = {High altitudes have been exposed to enhanced levels of surface ozone (O3) concentrations over recent decades compared to the pre-industrial era. The responses of vegetation to this toxic pollutant are species-specifc and depend on the climate conditions. In this paper, we explored the reaction of Pinus mugo (P. mugo) to O3-induced stress in the continental climate of an ozone-rich mountain area in the High Tatra Mountains (Western Carpathians). The efects of O3 doses modelled by a deposition model, O3 concentrations and other factors on P. mugo were identifed from (a) satellite-based data via NDVI (normalised diferenced vegetation index) over 2000–2020 and (b) visible injury on needle samples gathered from P. mugo individuals at ground-truth sites in 2019 and 2020. Analysing the NDVI trend, we observed non-signifcant changes (p>0.05) in the greenness of P. mugo despite growing in an environment with the average seasonal O3 concentration around 51.6 ppbv, the maximum hourly concentrations more than 90 ppbv and increasing trend of O3 doses by 0.1 mmol m−2 PLA (plant leaf area) year−1. The visible O3 injury of samples collected at study sites was low (mean injury observed on 1–10% of needles’ surface), and the symptoms of injury caused by other biotic and abiotic factors prevailed over those caused by O3. In addition, the correlation analyses between NDVI and the climatic factors indicated a signifcant (p<0.05) and positive relationship with photosynthetic active radiation (R=0.45) in July, and with stomatal conductance (R=0.52) and temperature factor (R=0.43) in August. Therefore, we concluded that the positive efect of climate conditions, which support the growth processes of P. mugo, may suppress the negative efect of the mean O3 doses of 17.8 mmol m−2 PLA accumulated over the growing season.}, keywords = {}, pubstate = {published}, tppubtype = {article} } High altitudes have been exposed to enhanced levels of surface ozone (O3) concentrations over recent decades compared to the pre-industrial era. The responses of vegetation to this toxic pollutant are species-specifc and depend on the climate conditions. In this paper, we explored the reaction of Pinus mugo (P. mugo) to O3-induced stress in the continental climate of an ozone-rich mountain area in the High Tatra Mountains (Western Carpathians). The efects of O3 doses modelled by a deposition model, O3 concentrations and other factors on P. mugo were identifed from (a) satellite-based data via NDVI (normalised diferenced vegetation index) over 2000–2020 and (b) visible injury on needle samples gathered from P. mugo individuals at ground-truth sites in 2019 and 2020. Analysing the NDVI trend, we observed non-signifcant changes (p>0.05) in the greenness of P. mugo despite growing in an environment with the average seasonal O3 concentration around 51.6 ppbv, the maximum hourly concentrations more than 90 ppbv and increasing trend of O3 doses by 0.1 mmol m−2 PLA (plant leaf area) year−1. The visible O3 injury of samples collected at study sites was low (mean injury observed on 1–10% of needles’ surface), and the symptoms of injury caused by other biotic and abiotic factors prevailed over those caused by O3. In addition, the correlation analyses between NDVI and the climatic factors indicated a signifcant (p<0.05) and positive relationship with photosynthetic active radiation (R=0.45) in July, and with stomatal conductance (R=0.52) and temperature factor (R=0.43) in August. Therefore, we concluded that the positive efect of climate conditions, which support the growth processes of P. mugo, may suppress the negative efect of the mean O3 doses of 17.8 mmol m−2 PLA accumulated over the growing season. |
 | Jančovičová, S; Adamčíková, K; Caboň, M; Adamčík, S Phylogeny of Crepidotus applanatus Look-Alikes Reveals a Convergent Morphology Evolution and a New Species C. pini Journal Article Journal of Fungi, 8 (489), 2022, ISSN: 2309-608X. @article{Jančovičová2022, title = {Phylogeny of Crepidotus applanatus Look-Alikes Reveals a Convergent Morphology Evolution and a New Species C. pini}, author = {S. Jančovičová and K. Adamčíková and M. Caboň and S. Adamčík}, url = {http://ife.sk/wp-content/uploads/2020/09/figure-2-5.jpg}, doi = {doi.org/10.3390/jof8050489}, issn = { 2309-608X}, year = {2022}, date = {2022-05-07}, journal = {Journal of Fungi}, volume = {8}, number = {489}, abstract = {Crepidotus applanatus is known as a common wood inhabiting fungus widely distributed throughout the Northern Hemisphere. There have been contrasting opinions about the delimitation and taxonomic treatment of the similar species C. malachius. Our phylogeny did not support the close relationship of these two morphologically similar species and the grouping of collections labelled by both names within each phylogenetic species reflects unreliable species delimitations in the traditional literatures. Both species inhabit the wood of deciduous trees, and our morphological analysis identified the size of basidiospores as a significant difference between them. The collections from Pinus sylvestris are recognised as a new species, C. pini sp. nov., and its morphological identification requires a combination of both basidiospore and cheilocystidia characters.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Crepidotus applanatus is known as a common wood inhabiting fungus widely distributed throughout the Northern Hemisphere. There have been contrasting opinions about the delimitation and taxonomic treatment of the similar species C. malachius. Our phylogeny did not support the close relationship of these two morphologically similar species and the grouping of collections labelled by both names within each phylogenetic species reflects unreliable species delimitations in the traditional literatures. Both species inhabit the wood of deciduous trees, and our morphological analysis identified the size of basidiospores as a significant difference between them. The collections from Pinus sylvestris are recognised as a new species, C. pini sp. nov., and its morphological identification requires a combination of both basidiospore and cheilocystidia characters. |
 | Buyck, B; Eyssartier, G; Armada, F; Corrales, A; Hembrom, M E; Rossi, W; Bellanger, J M; Das, K; Dima, B; Ghosh, A; Noordeloos, M E; Parihar, A; Krisai-Greilhuber, I; M., Leonardi M; Manz, C; Vera, M; Vila, J; Adamčíková, K; Bizio, E; Caboň, M; Hampe, F; Piepenbring, M; Adamčík, S Fungal Biodiversity Profiles 111-120 Journal Article Cryptogamie. Mycologie, 43 (2), pp. 23-61, 2022, ISSN: 1776-100. @article{Buyck2022, title = {Fungal Biodiversity Profiles 111-120}, author = {B. Buyck and G. Eyssartier and F. Armada and A. Corrales and M.E. Hembrom and W. Rossi and J.M. Bellanger and K. Das and B. Dima and A. Ghosh and M.E. Noordeloos and A. Parihar and I. Krisai-Greilhuber and M. Leonardi M. and C. Manz and M. Vera and J. Vila and K. Adamčíková and E. Bizio and M. Caboň and F. Hampe and M. Piepenbring and S. Adamčík }, url = {https://doi.org/10.5252/cryptogamie-mycologie2022v43a2. http://cryptogamie.com/mycologie/43/2}, doi = {doi.org/10.5252/cryptogamie-mycologie2022v43a2. }, issn = { 1776-100}, year = {2022}, date = {2022-03-03}, journal = {Cryptogamie. Mycologie}, volume = {43}, number = {2}, pages = {23-61}, abstract = {In this new series of Fungal Biodiversity Profiles, the authors provide descriptions for ten new taxa: Ilytheomyces uncinatus W. Rossi & M. Leonardi, sp. nov. (Ascomycota, Laboulbeniales) and several Basidiomycota: in family Entolomataceae for Entoloma aurantioalpinum Armada, Vila, Bellanger, Noordel., Krisai & Dima, sp. nov. and E. nigroflavescens Armada, Bellanger, Noordel. & Dima, sp. nov., both from France; in family Inocybaceae for Inocybe hebes Eyssart. & Buyck, sp. nov., I. media Eyssart. & Buyck, sp. nov. and I. leucophaea Eyssart. & Buyck, sp. nov., all three gathered from miombo woodland in Zambia (Africa); in family Russulaceae, Russula ferruginea subsp. ferruginea Corrales & Vera, sp. nov. and subsp. panamanensis Corrales & Manz, subsp. nov. from Central America; in Laetiporaceae Jülich for the Indian Phaeolus sharmae Hembrom, A. Parihar, K. Das & A. Ghosh, sp. nov.; in family Vuilleminiaceae Maire for Vuilleminia tropica Hembrom, A. Ghosh, A. Parihar & K. Das, sp. nov., equally from India. All new species of Basidiomycota are supported by newly provided sequence data or already published phylogenetic analyses of sequence data.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In this new series of Fungal Biodiversity Profiles, the authors provide descriptions for ten new taxa: Ilytheomyces uncinatus W. Rossi & M. Leonardi, sp. nov. (Ascomycota, Laboulbeniales) and several Basidiomycota: in family Entolomataceae for Entoloma aurantioalpinum Armada, Vila, Bellanger, Noordel., Krisai & Dima, sp. nov. and E. nigroflavescens Armada, Bellanger, Noordel. & Dima, sp. nov., both from France; in family Inocybaceae for Inocybe hebes Eyssart. & Buyck, sp. nov., I. media Eyssart. & Buyck, sp. nov. and I. leucophaea Eyssart. & Buyck, sp. nov., all three gathered from miombo woodland in Zambia (Africa); in family Russulaceae, Russula ferruginea subsp. ferruginea Corrales & Vera, sp. nov. and subsp. panamanensis Corrales & Manz, subsp. nov. from Central America; in Laetiporaceae Jülich for the Indian Phaeolus sharmae Hembrom, A. Parihar, K. Das & A. Ghosh, sp. nov.; in family Vuilleminiaceae Maire for Vuilleminia tropica Hembrom, A. Ghosh, A. Parihar & K. Das, sp. nov., equally from India. All new species of Basidiomycota are supported by newly provided sequence data or already published phylogenetic analyses of sequence data. |
 | Kobza, M; Ostrovský, R; Adamčíková, K; Pastirčáková, K Stability of trees infected by wood decay fungi estimated by acoustic tomography: a field survey Journal Article Trees Structure and Function, 36 (1), pp. 103-112, 2022, ISSN: 1432-2285. @article{Kobza2022, title = {Stability of trees infected by wood decay fungi estimated by acoustic tomography: a field survey}, author = {M. Kobza and R. Ostrovský and K. Adamčíková and K. Pastirčáková}, url = {https://doi.org/10.1007/s00468-021-02185-w}, doi = {10.1007/s00468-021-02185-w}, issn = {1432-2285}, year = {2022}, date = {2022-02-08}, journal = {Trees Structure and Function}, volume = {36}, number = {1}, pages = {103-112}, abstract = {In this survey, we assessed the relationships between the presence of visible fruiting bodies and measurements of both wood damage and tree safety factor (SF), the final aim being the determination of whether a tree showing wood decay signs should be felled or not. In general, trees affected by wood-decaying fungi cannot be considered risky prior to determining the impact of the fungi on their structural integrity. Trees of nine genera from 13 localities of Slovakia were included in this survey. In total, nineteen species of fungi were found causing wood decay on 74 individual host trees. Acoustic tomography was done using a Fakopp 3D tomograph, and SF was calculated by ArborSonic 3D software. The most commonly occurring taxon, Fomes fomentarius (L.) Fr., was found on six tree genera. The highest degree of damaged area on trunks was caused by fungi in the genera Fomes, Ganoderma, and Perenniporia (over 70%), while the lowest damage was caused by genera Spongipellis, Cerrena, and Auricularia (up to 30%). The lowest values of SF were measured on trees of the genus Acer infected by Fomes fomentarius; Prunus infected by Phellinus pomaceus (Pers.) Maire; and Fagus infected by Ganoderma pfeifferi Bres. Computation of the SF of individual trees plays a vital part in the stability evaluation of trees affected by wood decay fungi.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In this survey, we assessed the relationships between the presence of visible fruiting bodies and measurements of both wood damage and tree safety factor (SF), the final aim being the determination of whether a tree showing wood decay signs should be felled or not. In general, trees affected by wood-decaying fungi cannot be considered risky prior to determining the impact of the fungi on their structural integrity. Trees of nine genera from 13 localities of Slovakia were included in this survey. In total, nineteen species of fungi were found causing wood decay on 74 individual host trees. Acoustic tomography was done using a Fakopp 3D tomograph, and SF was calculated by ArborSonic 3D software. The most commonly occurring taxon, Fomes fomentarius (L.) Fr., was found on six tree genera. The highest degree of damaged area on trunks was caused by fungi in the genera Fomes, Ganoderma, and Perenniporia (over 70%), while the lowest damage was caused by genera Spongipellis, Cerrena, and Auricularia (up to 30%). The lowest values of SF were measured on trees of the genus Acer infected by Fomes fomentarius; Prunus infected by Phellinus pomaceus (Pers.) Maire; and Fagus infected by Ganoderma pfeifferi Bres. Computation of the SF of individual trees plays a vital part in the stability evaluation of trees affected by wood decay fungi. |
2021 |
|
 | Manz, C; Adamčík, S; Looney, B P; Corrales, A; Ovrebo, C; Adamčíková, K; Hofmann, T A; Hampe, F; Piepenbring, M Four new species of Russula subsection Roseinae from tropical montane forests in western Panama Journal Article PLoS ONE, 16 (10), pp. e0257616, 2021, ISSN: 1932-6203. @article{Manz2021, title = {Four new species of Russula subsection Roseinae from tropical montane forests in western Panama}, author = {C. Manz and S. Adamčík and B. P. Looney and A. Corrales and C. Ovrebo and K. Adamčíková and T. A. Hofmann and F. Hampe and M. Piepenbring }, url = {https://doi.org/10.1371/journal.pone.0257616}, doi = {doi.org/10.1371/journal.pone.0257616}, issn = {1932-6203}, year = {2021}, date = {2021-10-14}, journal = {PLoS ONE}, volume = {16}, number = {10}, pages = {e0257616}, abstract = {Species of the genus Russula are key components of ectomycorrhizal ecosystems worldwide. Nevertheless, their diversity in the tropics is still poorly known. This study aims to contribute to the knowledge of the diversity of Russula species classified in subsection Roseinae based on specimens recently collected in tropical montane rainforests in western Panama. A five gene multilocus phylogeny based on the nuclear markers ITS nrDNA, MCM7, RPB1, RPB2 and TEF-1α was constructed to identify the systematic position of 22 collections from Panama. Four new species, Russula cornicolor, Russula cynorhodon, Russula oreomunneae and Russula zephyrovelutipes are formally described and illustrated. None of the four species are sister species and they are more closely related to North American or Asian species. Two of the newly described species were associated with the ectomycorrhizal tree species Oreomunnea mexicana, while the other two species were associated with Quercus species. All four species are so far only known from mountains in western Panama.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species of the genus Russula are key components of ectomycorrhizal ecosystems worldwide. Nevertheless, their diversity in the tropics is still poorly known. This study aims to contribute to the knowledge of the diversity of Russula species classified in subsection Roseinae based on specimens recently collected in tropical montane rainforests in western Panama. A five gene multilocus phylogeny based on the nuclear markers ITS nrDNA, MCM7, RPB1, RPB2 and TEF-1α was constructed to identify the systematic position of 22 collections from Panama. Four new species, Russula cornicolor, Russula cynorhodon, Russula oreomunneae and Russula zephyrovelutipes are formally described and illustrated. None of the four species are sister species and they are more closely related to North American or Asian species. Two of the newly described species were associated with the ectomycorrhizal tree species Oreomunnea mexicana, while the other two species were associated with Quercus species. All four species are so far only known from mountains in western Panama. |
 | Lange, De R; Adamčík, S; Adamčíková, K; Asselman, P; Borovička, J; Delgat, L; Hampe, F; Verbeken, A Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex Journal Article IMA Fungus, 20 (Article ID 12), pp. 1-31, 2021, ISSN: 2210-6359. @article{Lange2020, title = {Enlightening the black and white: species delimitation and UNITE species hypothesis testing in the Russula albonigra species complex}, author = {R. De Lange and S. Adamčík and K. Adamčíková and P. Asselman and J. Borovička and L. Delgat and F. Hampe and A. Verbeken}, url = {https://imafungus.biomedcentral.com/articles/10.1186/s43008-021-00064-0}, doi = {10.1186/s43008-021-00064-0}, issn = {2210-6359}, year = {2021}, date = {2021-08-02}, journal = {IMA Fungus}, volume = {20}, number = {Article ID 12}, pages = {1-31}, abstract = {Russula albonigra is considered a well-known species, morphologically delimited by the context of the basidiomata blackening without intermediate reddening, and the menthol-cooling taste of the lamellae. It is supposed to have a broad ecological range and a large distribution area. A thorough molecular analysis based on four nuclear markers (ITS, LSU, RPB2 and TEF1-α) shows this traditional concept of R. albonigra s. lat. represents a species complex consisting of at least five European, three North American, and one Chinese species. Morphological study shows traditional characters used to delimit R. albonigra are not always reliable. Therefore, a new delimitation of the R. albonigra complex is proposed and a key to the described European species of R. subgen. Compactae is presented. A lectotype and an epitype are designated for R. albonigra and three new European species are described: R. ambusta, R. nigrifacta, and R. ustulata. Different thresholds of UNITE species hypotheses were tested against the taxonomic data. The distance threshold of 0.5% gives a perfect match to the phylogenetically defined species within the R. albonigra complex. Publicly available sequence data can contribute to species delimitation and increase our knowledge on ecology and distribution, but the pitfalls are short and low quality sequences.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Russula albonigra is considered a well-known species, morphologically delimited by the context of the basidiomata blackening without intermediate reddening, and the menthol-cooling taste of the lamellae. It is supposed to have a broad ecological range and a large distribution area. A thorough molecular analysis based on four nuclear markers (ITS, LSU, RPB2 and TEF1-α) shows this traditional concept of R. albonigra s. lat. represents a species complex consisting of at least five European, three North American, and one Chinese species. Morphological study shows traditional characters used to delimit R. albonigra are not always reliable. Therefore, a new delimitation of the R. albonigra complex is proposed and a key to the described European species of R. subgen. Compactae is presented. A lectotype and an epitype are designated for R. albonigra and three new European species are described: R. ambusta, R. nigrifacta, and R. ustulata. Different thresholds of UNITE species hypotheses were tested against the taxonomic data. The distance threshold of 0.5% gives a perfect match to the phylogenetically defined species within the R. albonigra complex. Publicly available sequence data can contribute to species delimitation and increase our knowledge on ecology and distribution, but the pitfalls are short and low quality sequences. |
 | Adamčíková, K; Jánošíková, Z; Adamčík, S; Ostrovský, R; Pastirčáková, K; Kobza, M; Ondrušková, E Host range, genetic variability, and mating types of Lecanosticta acicola in Slovakia Journal Article Scandinavian Journal of Forest Research, 36 (5), pp. 325-332, 2021, ISSN: 0282-7581. @article{Adamčíková2021b, title = {Host range, genetic variability, and mating types of \textit{Lecanosticta acicola} in Slovakia}, author = {K. Adamčíková and Z. Jánošíková and S. Adamčík and R. Ostrovský and K. Pastirčáková and M. Kobza and E. Ondrušková}, url = {https://doi.org/10.1080/02827581.2021.1941236}, doi = {10.1080/02827581.2021.1941236}, issn = {0282-7581}, year = {2021}, date = {2021-07-16}, journal = {Scandinavian Journal of Forest Research}, volume = {36}, number = {5}, pages = {325-332}, abstract = {In recent years, there has been an apparent Europe-wide emerging presence of brown spot needle blight, a disease of pine species caused by the fungus Lecanosticta acicola. In this study, we report the first well-documented occurrences of the pathogen in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region and TEF1 gene. Among the material collected from 84 locations within the country, L. acicola was present in 17 samples from 13 different locations, mainly distributed in urban environments. Four pine species were identified as hosts, among which, Pinus nigra and P. mugo were found to be the most frequently infected. Analysis of the mating type genes of 24 isolates obtained from two localities revealed the presence of a single mating type in Slovakia. All analyzed ITS sequences of the Slovak isolates were found to be uniform. However, although analysis of the TEF1 gene indicated that all Slovak isolates could be grouped into a single lineage, we detected nucleotide polymorphisms suggestive of a certain degree of genetic diversification within central European populations of the fungus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In recent years, there has been an apparent Europe-wide emerging presence of brown spot needle blight, a disease of pine species caused by the fungus Lecanosticta acicola. In this study, we report the first well-documented occurrences of the pathogen in Slovakia, identified molecularly using species-specific primers and based on sequencing of the ITS region and TEF1 gene. Among the material collected from 84 locations within the country, L. acicola was present in 17 samples from 13 different locations, mainly distributed in urban environments. Four pine species were identified as hosts, among which, Pinus nigra and P. mugo were found to be the most frequently infected. Analysis of the mating type genes of 24 isolates obtained from two localities revealed the presence of a single mating type in Slovakia. All analyzed ITS sequences of the Slovak isolates were found to be uniform. However, although analysis of the TEF1 gene indicated that all Slovak isolates could be grouped into a single lineage, we detected nucleotide polymorphisms suggestive of a certain degree of genetic diversification within central European populations of the fungus. |
 | Adamčíková, K; Jánošíková, Z; van der Nest, A; Adamčík, S; Ondrušková, E; Barnes, I Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia Journal Article Plant Pathology, 70 (8), pp. 1883-1896, 2021, ISSN: 1365-3059. @article{Adamčíková2021c, title = {Population structure and genetic diversity suggest recent introductions of Dothistroma pini in Slovakia}, author = {K. Adamčíková and Z. Jánošíková and A. van der Nest and S. Adamčík and E. Ondrušková and I. Barnes }, url = {https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13428}, doi = {10.1111/ppa.13428}, issn = {1365-3059}, year = {2021}, date = {2021-06-30}, journal = {Plant Pathology}, volume = {70}, number = {8}, pages = {1883-1896}, abstract = {Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma pini is one of two pathogens causing Dothistroma needle blight (DNB), a foliar disease of pines. The species was redefined in 2004 and subsequently recorded in several European countries. In Slovakia, the first report of the pathogen was in 2013. In this study, the population structure, genetic diversity, and reproductive mode of 105 isolates collected from 10 localities and seven hosts were determined in Slovakia. Species-specific mating type markers, ITS haplotype determination, and 16 microsatellite markers were used to characterize and genotype the isolates. Overall, 15 unique multilocus haplotypes (MLHs) based on microsatellite markers and three ITS haplotypes were identified. Three independent methods (DAPC, STRUCTURE, EDENetwork) separated the isolates into two distinct population clusters corresponding with ITS haplotypes. A high level of clonality was recorded suggesting that conidia are the primary source of pathogen dispersal. The low genetic diversity, predominantly asexual reproductive mode of the pathogen, and the fact that most isolates were collected from introduced tree species and native species in artificially planted urban greenery, supports the hypothesis that D. pini has been recently introduced into Slovakia. |
 | Jánošíková, Z; Dutech, C; Ondrušková, E; Adamčíková, K; Mullett, M Population structure and genetic diversity of Dothistroma septosporum in Slovakia Journal Article European Journal of Plant Pathology, 160 (4), pp. 771-787, 2021, ISSN: 1573-8469. @article{Jánošíková2021, title = {Population structure and genetic diversity of Dothistroma septosporum in Slovakia}, author = {Z. Jánošíková and C. Dutech and E. Ondrušková and K. Adamčíková and M. Mullett}, url = {https://link.springer.com/article/10.1007/s10658-021-02266-z}, doi = {doi:10.1007/s10658-021-02266-z}, issn = {1573-8469}, year = {2021}, date = {2021-06-23}, journal = {European Journal of Plant Pathology}, volume = {160}, number = {4}, pages = {771-787}, abstract = {Dothistroma needle blight (DNB) is a serious pine disease present worldwide caused by the ascomycetous fungi Dothistroma septosporum and Dothistroma pini. Based on multiplex PCR analysis of 11 microsatellite markers screened on 253 D. septosporum isolates obtained from 32 sites across Slovakia, a total of 137 unique multilocus haplotypes (MLHs) were detected. The majority of MLHs (n = 91) were represented by a single isolate, but 13 MLHs were found at two different sites, and in some cases, these were separated by large distances. Four population clusters were identified using a discriminant analysis of principal components (DAPC). The genetic clusters obtained from the DAPC were mixed throughout the country and were not restricted to particular host species. Although both mating types of D. septosporum were found to be in an almost perfect 1:1 ratio in both the non-clone-corrected and clone-corrected datasets, random mating was rejected in the entire dataset. Random mating was only supported in the Pinus mugo group planted in urban areas and on a smaller spatial scale in two sites (Kálnica and Litava) using the clonecorrected dataset. These findings suggest a mixed mode of reproduction with an important component of sexual reproduction, although the sexual stage of the fungus (teleomorph) has not been physically observed in Slovakia yet. The examination of spatial relationships using spatial principal component analyses and the presence of isolation-by-distance together with relatively high genetic diversity suggests the pathogen has been long established in Slovakia and spread naturally across the landscape. However, the weak population structure and findings of identical clones at widely separated sites strongly suggests some degree of human assisted dispersal.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a serious pine disease present worldwide caused by the ascomycetous fungi Dothistroma septosporum and Dothistroma pini. Based on multiplex PCR analysis of 11 microsatellite markers screened on 253 D. septosporum isolates obtained from 32 sites across Slovakia, a total of 137 unique multilocus haplotypes (MLHs) were detected. The majority of MLHs (n = 91) were represented by a single isolate, but 13 MLHs were found at two different sites, and in some cases, these were separated by large distances. Four population clusters were identified using a discriminant analysis of principal components (DAPC). The genetic clusters obtained from the DAPC were mixed throughout the country and were not restricted to particular host species. Although both mating types of D. septosporum were found to be in an almost perfect 1:1 ratio in both the non-clone-corrected and clone-corrected datasets, random mating was rejected in the entire dataset. Random mating was only supported in the Pinus mugo group planted in urban areas and on a smaller spatial scale in two sites (Kálnica and Litava) using the clonecorrected dataset. These findings suggest a mixed mode of reproduction with an important component of sexual reproduction, although the sexual stage of the fungus (teleomorph) has not been physically observed in Slovakia yet. The examination of spatial relationships using spatial principal component analyses and the presence of isolation-by-distance together with relatively high genetic diversity suggests the pathogen has been long established in Slovakia and spread naturally across the landscape. However, the weak population structure and findings of identical clones at widely separated sites strongly suggests some degree of human assisted dispersal. |
 | Vera, M; Adamčík, S; Adamčíková, K; Hampe, F; Caboň, M; Manz, C; Overbo, C; Peipenbring, M; Corrales, A Morphological and genetic diversification of Russula floriformis, sp. nov., along the Isthmus of Panama Journal Article Mycologia, 113 (4), pp. 807-827, 2021, ISSN: 1557-2536. @article{Vera2021, title = {Morphological and genetic diversification of Russula floriformis, sp. nov., along the Isthmus of Panama}, author = {M. Vera and S. Adamčík and K. Adamčíková and F. Hampe and M. Caboň and C. Manz and C. Overbo and M. Peipenbring and A. Corrales}, url = {https://www.tandfonline.com/doi/full/10.1080/00275514.2021.1897377}, doi = {DOI: 10.1080/00275514.2021.1897377}, issn = {1557-2536}, year = {2021}, date = {2021-05-27}, journal = {Mycologia}, volume = {113}, number = {4}, pages = {807-827}, abstract = {Species of Russula are ubiquitous members of ectomycorrhizal fungal communities in tropical ecosystems. However, an important part of the total tropical diversity of this genus and its biogeographic patterns is unknown due to the lack of studies on Russula in tropical ecosystems. We combined molecular, morphological, ecological, and biogeographic data to elaborate concepts for two new subspecies of R. floriformis (subsection Substriatinae). Russula floriformis subsp. floriformis and R. floriformis subsp. symphoniae are described as new from montane forest dominated by Quercus and/or Oreomunnea (Fagales) from Colombia and Panama, respectively. Phylogenies were constructed using nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), D1–D2 domains of nuc 28S rDNA (28S), and partial regions of the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-alpha (tef1). Similar environmental conditions, similar morphology, and an ITS sequence similarity higher than 99% with only three different positions indicate that these two subspecies are closely related. Detailed observations of microscopic structures and analyses of further DNA loci, however, revealed morphological and molecular characteristics that allow distinguishing the two subspecies of R. floriformis. Spatial distribution and phylogenetic proximity of the two Russula subspecies and their ectomycorrhizal hosts, i.e., species of Quercus, suggest that their diversification is a result of comigration, adaptation, and geographic isolation along the Isthmus of Panama during the Pliocene and Pleistocene.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Species of Russula are ubiquitous members of ectomycorrhizal fungal communities in tropical ecosystems. However, an important part of the total tropical diversity of this genus and its biogeographic patterns is unknown due to the lack of studies on Russula in tropical ecosystems. We combined molecular, morphological, ecological, and biogeographic data to elaborate concepts for two new subspecies of R. floriformis (subsection Substriatinae). Russula floriformis subsp. floriformis and R. floriformis subsp. symphoniae are described as new from montane forest dominated by Quercus and/or Oreomunnea (Fagales) from Colombia and Panama, respectively. Phylogenies were constructed using nuc rDNA internal transcribed spacer region ITS1-5.8S-ITS2 (ITS), D1–D2 domains of nuc 28S rDNA (28S), and partial regions of the second largest subunit of RNA polymerase II (rpb2) and translation elongation factor 1-alpha (tef1). Similar environmental conditions, similar morphology, and an ITS sequence similarity higher than 99% with only three different positions indicate that these two subspecies are closely related. Detailed observations of microscopic structures and analyses of further DNA loci, however, revealed morphological and molecular characteristics that allow distinguishing the two subspecies of R. floriformis. Spatial distribution and phylogenetic proximity of the two Russula subspecies and their ectomycorrhizal hosts, i.e., species of Quercus, suggest that their diversification is a result of comigration, adaptation, and geographic isolation along the Isthmus of Panama during the Pliocene and Pleistocene. |
 | Adamčíková, K; Ondrušková, E; Pažitný, J; Jánošíková, Z Divergence in culture morphology between two related species, Dothistroma septosporum and D. pini Journal Article Czech Mycology, 73 (1), pp. 109-119, 2021, ISSN: 1805-1421. @article{Adamčíková2021, title = {Divergence in culture morphology between two related species, Dothistroma septosporum and D. pini}, author = {K. Adamčíková and E. Ondrušková and J. Pažitný and Z. Jánošíková}, url = {http://ife.sk/wp-content/uploads/2020/09/fig.-2-rev-5.tif}, doi = {doi.org/10.33585/cmy.73108}, issn = {1805-1421}, year = {2021}, date = {2021-05-24}, journal = {Czech Mycology}, volume = {73}, number = {1}, pages = {109-119}, abstract = {Dothistroma needle blight (DNB) is one of the most important pine needle diseases worldwide. The disease is caused by two fungal species, Dothistroma septosporum and D. pini, which are similar not only in terms of their ecology, causing similar symptoms in hosts of the genus Pinus, but also in terms of their morphological characteristics. The morphologies of 353 cultured Dothistroma isolates from 11 different pine species in Slovakia and their relation to Dothistroma species and/or fungus origin (host) were studied and evaluated by means of the Chi-square test. The cultures were classified into eight categories according to pigmentation. Dothistroma pini isolates were assigned to 4 of the 8 categories, and D. septosporum isolates were assigned to all 8 categories with varying frequencies. The Chi-square test revealed that the culture morphology categories of D. pini and D. septosporum differed significantly. Interactions between the evaluated factors (culture morphology, Dothistroma and host species) were analysed and showed significant differences.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is one of the most important pine needle diseases worldwide. The disease is caused by two fungal species, Dothistroma septosporum and D. pini, which are similar not only in terms of their ecology, causing similar symptoms in hosts of the genus Pinus, but also in terms of their morphological characteristics. The morphologies of 353 cultured Dothistroma isolates from 11 different pine species in Slovakia and their relation to Dothistroma species and/or fungus origin (host) were studied and evaluated by means of the Chi-square test. The cultures were classified into eight categories according to pigmentation. Dothistroma pini isolates were assigned to 4 of the 8 categories, and D. septosporum isolates were assigned to all 8 categories with varying frequencies. The Chi-square test revealed that the culture morphology categories of D. pini and D. septosporum differed significantly. Interactions between the evaluated factors (culture morphology, Dothistroma and host species) were analysed and showed significant differences. |
 | Pastirčáková, K; Adamčík, S; Adamčíková, K; Chater, A O Erysiphe hypophylla, a second powdery mildew (Erysiphales) on oaks in Britain Journal Article Field Mycology, 22 (2), pp. 50-54, 2021, ISSN: 1468-1641. @article{Pastirčáková2021b, title = {\textit{Erysiphe hypophylla}, a second powdery mildew (\textit{Erysiphales}) on oaks in Britain}, author = {K. Pastirčáková and S. Adamčík and K. Adamčíková and A.O. Chater}, doi = {10.1016/j.fldmyc.2021.04.008}, issn = {1468-1641}, year = {2021}, date = {2021-04-20}, journal = {Field Mycology}, volume = {22}, number = {2}, pages = {50-54}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
 | Mullett, M S; Drenkhan, R; Adamson, K; Boroń, P; Lenart-Boroń, A; Barnes, I; Tomšovský, M; Jánošíková, Z; Adamčíková, K; Ondrušková, E; Queloz, V; Piškur, B; Musolin, D L; Davydenko, K; Georgieva, M; Schmitz, S; Kačergius, A; Ghelardini, L; Orlović, Kranjec J; Müller, M; Oskay, F; Hauptman, T; Halász, Á; Markovskaja, S; Solheim, H; Vuorinen, M; Heinzelmann, R; Hamelin, R C; Konečný, A Journal of Fungi, 7 (2), pp. Article number: 111, 2021, ISBN: 2309-608X. @article{Mullett2021, title = {Worldwide Genetic Structure Elucidates the Eurasian Origin and Invasion Pathways of Dothistroma septosporum, Causal Agent of Dothistroma Needle Blight }, author = {M. S. Mullett and R. Drenkhan and K. Adamson and P. Boroń and A. Lenart-Boroń and I. Barnes and M. Tomšovský and Z. Jánošíková and K. Adamčíková and E. Ondrušková and V. Queloz and B. Piškur and D.L. Musolin and K. Davydenko and M. Georgieva and S. Schmitz and A. Kačergius and L. Ghelardini and J. Kranjec Orlović and M. Müller and F. Oskay and T. Hauptman and Á. Halász and S. Markovskaja and H. Solheim and M. Vuorinen and R. Heinzelmann and R.C. Hamelin and A. Konečný}, doi = {doi 10.3390/jof7020111 }, isbn = {2309-608X}, year = {2021}, date = {2021-02-03}, journal = {Journal of Fungi}, volume = {7}, number = {2}, pages = {Article number: 111}, abstract = {Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia. |
 | Sánchez-García, M; Adamčíková, K; Moreau, P -A; Vizzini, A; Jančovičová, S; Kiran, M; Caboň, M; Matheny, P B; Adamčík, S The genus Dermoloma is more diverse than expected and forms a monophyletic lineage in the Tricholomataceae Journal Article Mycological Progress, 20 (1), pp. 11–25, 2021, ISSN: 1617-416X. @article{Sánchez-García2021, title = {The genus Dermoloma is more diverse than expected and forms a monophyletic lineage in the Tricholomataceae}, author = {M. Sánchez-García and K. Adamčíková and P.-A. Moreau and A. Vizzini and S. Jančovičová and M. Kiran and M. Caboň and P. B. Matheny and S. Adamčík}, doi = {doi 10.1007/s11557-020-01651-y}, issn = {1617-416X}, year = {2021}, date = {2021-01-18}, journal = {Mycological Progress}, volume = {20}, number = {1}, pages = {11–25}, abstract = {We present the first phylogenetic evaluation of the genus Dermoloma, which is resolved as monophyletic and closely related to Pseudotricholoma, a poorly known Dermoloma-like lineage within the family Tricholomataceae. The position of Dermoloma is confirmed by the placement of the type species, Dermoloma cuneifolium, represented by multiple samples including the neotype. Based on our phylogenetic analyses, we recognised 25 European operational taxonomic units (OTUs) but could only assign species names to ten of them based on ex-type sequences. Furthermore, only five additional published Dermoloma names of uncertain status are available for the remaining 16 potential European species, thus demonstrating an unexpected amount of taxonomic diversity. Samples from Europe and North America seem to be endemic on a continental scale. North American samples formed six unique OTUs, but only one could be reliably named, Dermoloma hymenocephalum. Dermoloma is morphologically defined by basidiomata with brown, grey and white colours with a farinaceous odour and a pluristratous hymeniderm type of pileipellis. Our phylogenetic analyses support the subdivision of the genus into two subgenera and four sections, and species with inamyloid basidiospores are placed in subg. Dermoloma and those with amyloid basidiospores in subg. Amylospora. Both subgenera are further divided into two sections. The analysis of spore morphology shows that sect. Conica of subg. Dermoloma and sect. Nigrescentia of subg. Amylospora have a very distinctive spore shape. Sect. Atrobrunnea of subg. Amylospora showed relatively high variability of spores among species, but spores of sect. Dermoloma were similar and not useful for species discrimination.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We present the first phylogenetic evaluation of the genus Dermoloma, which is resolved as monophyletic and closely related to Pseudotricholoma, a poorly known Dermoloma-like lineage within the family Tricholomataceae. The position of Dermoloma is confirmed by the placement of the type species, Dermoloma cuneifolium, represented by multiple samples including the neotype. Based on our phylogenetic analyses, we recognised 25 European operational taxonomic units (OTUs) but could only assign species names to ten of them based on ex-type sequences. Furthermore, only five additional published Dermoloma names of uncertain status are available for the remaining 16 potential European species, thus demonstrating an unexpected amount of taxonomic diversity. Samples from Europe and North America seem to be endemic on a continental scale. North American samples formed six unique OTUs, but only one could be reliably named, Dermoloma hymenocephalum. Dermoloma is morphologically defined by basidiomata with brown, grey and white colours with a farinaceous odour and a pluristratous hymeniderm type of pileipellis. Our phylogenetic analyses support the subdivision of the genus into two subgenera and four sections, and species with inamyloid basidiospores are placed in subg. Dermoloma and those with amyloid basidiospores in subg. Amylospora. Both subgenera are further divided into two sections. The analysis of spore morphology shows that sect. Conica of subg. Dermoloma and sect. Nigrescentia of subg. Amylospora have a very distinctive spore shape. Sect. Atrobrunnea of subg. Amylospora showed relatively high variability of spores among species, but spores of sect. Dermoloma were similar and not useful for species discrimination. |
 | Pastirčáková, Katarína; Adamčíková, Katarína; Bacigálová, Kamila; Caboň, Miroslav; Mikušová, Petra; Senko, Dušan; Svitok, Marek; Adamčík, Slavomír Ash Trees (Fraxinus spp.) in Urban Greenery as Possible Invasion Gates of Non-Native Phyllactinia Species Journal Article Forests, 12 (2), pp. 183, 2021, ISSN: 1999-4907. @article{Pastir_kov__2021, title = {Ash Trees (Fraxinus spp.) in Urban Greenery as Possible Invasion Gates of Non-Native Phyllactinia Species}, author = {Katarína Pastirčáková and Katarína Adamčíková and Kamila Bacigálová and Miroslav Caboň and Petra Mikušová and Dušan Senko and Marek Svitok and Slavomír Adamčík}, url = {http://dx.doi.org/10.3390/f12020183}, doi = {10.3390/f12020183}, issn = {1999-4907}, year = {2021}, date = {2021-01-01}, journal = {Forests}, volume = {12}, number = {2}, pages = {183}, publisher = {MDPI AG}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2020 |
|
 | Bičárová, S; Shashikumar, A; Richier, Dalstein- L; Lukasová, V; Adamčíková, K; Pavlendová, H; Sitková, Z; Buchholcerová, A; Bilčík, D The response of Pinus species to ozone uptake in different climate regions of Europe Journal Article Central European Forestry Journal , 66 (4), pp. 255–268, 2020, ISBN: 2454-034X. @article{Bičárová2020, title = {The response of Pinus species to ozone uptake in different climate regions of Europe}, author = {S. Bičárová and A. Shashikumar and L. Dalstein- Richier and V. Lukasová and K. Adamčíková and H. Pavlendová and Z. Sitková and A. Buchholcerová and D. Bilčík}, doi = {DOI: 10.2478/forj-2020-0022}, isbn = {2454-034X}, year = {2020}, date = {2020-12-04}, journal = {Central European Forestry Journal }, volume = {66}, number = {4}, pages = {255–268}, abstract = {This study is focused on the research of selected Pinus species exposed to high ozone concentrations in the mountain environment. We noticed different values of modelled ozone doses (MOD) up-taken by Mountain pine (Pinus mugo Turra) in the High Tatra Mts (SK–HTMts) and Swiss stone pine (Pinus cembra L.) in the Alpes-Mercantour (FR–AlpMar) during the growing season 2019. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. Testing has shown that ozone input based on passive sampling may be used in MOD modelling for sites situated in the subalpine zone where the operation of active monitors is limited. . Presented results confirmed the assumption regarding stomatal ozone flux reduction due to the occurrence of soil drought in hot and dry summer weather typical for the Mediterranean climate region. Despite the limitation of stomatal flux, foliar ozone specific injury on two years needles of P. cembra was substantially higher in comparison to the incidence of ozone injury symptoms observed on two years needles of P. mugo in SK–HTMts. It may suggest low phytotoxicity of given MOD or efficient resistance of P. mugo against oxidative stress. In addition, the visible injury index (VINX) covering the broad effect of biotic and abiotic harmful agents was appraised on P. mugo. Percentage of affected surface indicated moderate deterioration of needle injury at the end of the growing season, particularly due to traces of mechanical damage.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study is focused on the research of selected Pinus species exposed to high ozone concentrations in the mountain environment. We noticed different values of modelled ozone doses (MOD) up-taken by Mountain pine (Pinus mugo Turra) in the High Tatra Mts (SK–HTMts) and Swiss stone pine (Pinus cembra L.) in the Alpes-Mercantour (FR–AlpMar) during the growing season 2019. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. The MOD values were obtained by multiplicative DO3SE model, while we also tested a new approach based on modification of input ozone data. Testing has shown that ozone input based on passive sampling may be used in MOD modelling for sites situated in the subalpine zone where the operation of active monitors is limited. . Presented results confirmed the assumption regarding stomatal ozone flux reduction due to the occurrence of soil drought in hot and dry summer weather typical for the Mediterranean climate region. Despite the limitation of stomatal flux, foliar ozone specific injury on two years needles of P. cembra was substantially higher in comparison to the incidence of ozone injury symptoms observed on two years needles of P. mugo in SK–HTMts. It may suggest low phytotoxicity of given MOD or efficient resistance of P. mugo against oxidative stress. In addition, the visible injury index (VINX) covering the broad effect of biotic and abiotic harmful agents was appraised on P. mugo. Percentage of affected surface indicated moderate deterioration of needle injury at the end of the growing season, particularly due to traces of mechanical damage. |
 | Adamčíková, K; Jánošíková, Z; Pažitný, J Inhibition effect of selected inorganic metal ions on the mycelial growth of Cryphonectria parasitica Journal Article Journal of Plant Protection Research, 60 (4), pp. 399-405, 2020, ISSN: 1427-4345. @article{Adamčíková2020, title = {Inhibition effect of selected inorganic metal ions on the mycelial growth of Cryphonectria parasitica}, author = {K. Adamčíková and Z. Jánošíková and J. Pažitný}, doi = {DOI: 10.24425/jppr.2020.134915}, issn = {1427-4345}, year = {2020}, date = {2020-12-02}, journal = {Journal of Plant Protection Research}, volume = {60}, number = {4}, pages = {399-405}, abstract = {In the current study the antifungal activity of inorganic reagents was tested against Cryphonectria parasitica in vitro in a mycelial growth inhibition test. Three reagents, each consisting of chloride silver (AgCl) in combination with (1) aluminum oxide, (2) zinc oxide − ZnO, and (3) aluminum oxide and titanium dioxide were tested. Significant differences of the tested reagents on the growth of C. parasitica were recorded. The study demonstrated that silver in mixture with ZnO had an antifungal effect and significantly reduced the mycelial growth of C. parasitica in vitro. The mixture of AgCl with the other two combinations of inorganic metal oxides had no inhibition effect on the growth of the pathogen. It was confirmed that ZnO (applied in a single compound test) is responsible for inhibition of C. parasitica mycelium growth. A preliminary in planta assay was performed but statistically significant differences were not recorded in the average increment of canker length.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the current study the antifungal activity of inorganic reagents was tested against Cryphonectria parasitica in vitro in a mycelial growth inhibition test. Three reagents, each consisting of chloride silver (AgCl) in combination with (1) aluminum oxide, (2) zinc oxide − ZnO, and (3) aluminum oxide and titanium dioxide were tested. Significant differences of the tested reagents on the growth of C. parasitica were recorded. The study demonstrated that silver in mixture with ZnO had an antifungal effect and significantly reduced the mycelial growth of C. parasitica in vitro. The mixture of AgCl with the other two combinations of inorganic metal oxides had no inhibition effect on the growth of the pathogen. It was confirmed that ZnO (applied in a single compound test) is responsible for inhibition of C. parasitica mycelium growth. A preliminary in planta assay was performed but statistically significant differences were not recorded in the average increment of canker length. |
 | Ondrušková, Emília; Ostrovský, Radovan; Jánošíková, Zuzana; Adamčíková, Katarína; Kobza, Marek Selected climatic variables in Slovakia are favourable to the development of Dothistroma needle blight Journal Article Folia Oecologica, 47 (2), pp. 144-152, 2020. @article{Ondrušková2020b, title = {Selected climatic variables in Slovakia are favourable to the development of Dothistroma needle blight}, author = {Emília Ondrušková and Radovan Ostrovský and Zuzana Jánošíková and Katarína Adamčíková and Marek Kobza}, url = {http://ife.sk/wp-content/uploads/2020/09/foecol-2020-0008.pdf}, doi = {10.2478/foecol-2020-0017}, year = {2020}, date = {2020-11-24}, journal = {Folia Oecologica}, volume = {47}, number = {2}, pages = {144-152}, abstract = {Dothistroma needle blight (DNB) is a disease of pine needles. It causes significant defoliation of affected trees; intensive attacks lead to damages of the whole stand. The relationship of trends in disease severity and intensity with climatic variables were studied in three Austrian pine plantations (Jahodná, Kálnica, Litava) during 2014–2018. During the monitoring period, the greatest variability in disease severity was observed in the top third of the crowns, which showed the highest correlation with the variants of the most important climatic conditions (temperature and relative humidity) tested. For the spread of DNB, based on statistical assessment, a higher number of intervals of environmentally favourable climatic conditions is crucial. Both Dothistroma species (D. septosporum and D. pini), which are causal agents of the disease, were identified in Jahodná. In Kálnica and Litava, only D. septosporum was present.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dothistroma needle blight (DNB) is a disease of pine needles. It causes significant defoliation of affected trees; intensive attacks lead to damages of the whole stand. The relationship of trends in disease severity and intensity with climatic variables were studied in three Austrian pine plantations (Jahodná, Kálnica, Litava) during 2014–2018. During the monitoring period, the greatest variability in disease severity was observed in the top third of the crowns, which showed the highest correlation with the variants of the most important climatic conditions (temperature and relative humidity) tested. For the spread of DNB, based on statistical assessment, a higher number of intervals of environmentally favourable climatic conditions is crucial. Both Dothistroma species (D. septosporum and D. pini), which are causal agents of the disease, were identified in Jahodná. In Kálnica and Litava, only D. septosporum was present. |
 | Drenkhan, R; Ganley, B; Martín-García, J; Vahalík, P; Adamson, K; Adamčíková, K; Ahumada, R; Blank, L; Bragança, H; Capretti, P; Cleary, M; Cornejo, C; Davydenko, K; Diez, J J; Lehtijärvi, Doğmuş H T; Dvořák, M; Enderle, R; Fourie, G; Georgieva, M; Ghelardini, L; Hantula, J; Ioos, R; Iturritxa, E; Kanetis, L; Karpun, N N; Koltay, A; Landeras, E; Markovskaja, S; Mesanza, N; Milenković, I; Musolin, D L; Nikolaou, K; Nowakowska, J A; Ogris, N; Oskay, F; Oszako, T; Papazova-Anakieva, I; Paraschiv, M; Pasquali, M; Pecori, F; Rafoss, T; Raitelaitytė, K; Raposo, R; Robin, C; Rodas, C A; A., Santini A; Sanz-Ros, A V; Selikhovkin, A V; Solla, A; Soukainen, M; Soulioti, N; Steenkamp, E T; Tsopelas, P; Vemić, A; Vettraino, A M; Wingfield, M J; Woodward, S; C., Zamora-Ballesteros C; Mullett, M S Global Geographic Distribution and Host Range of Fusarium circinatum, the Causal Agent of Pine Pitch Canker Journal Article Forests, 11 (7), 2020, ISSN: 1999-4907. @article{Drenkhan2020, title = {Global Geographic Distribution and Host Range of Fusarium circinatum, the Causal Agent of Pine Pitch Canker}, author = {R. Drenkhan and B. Ganley and J. Martín-García and P. Vahalík and K. Adamson and K. Adamčíková and R. Ahumada and L. Blank and H. Bragança and P. Capretti and M. Cleary and C. Cornejo and K. Davydenko and J.J. Diez and H.T. Doğmuş Lehtijärvi and M. Dvořák and R. Enderle and G. Fourie and M. Georgieva and L. Ghelardini and J. Hantula and R. Ioos and E. Iturritxa and L. Kanetis and N.N. Karpun and A. Koltay and E. Landeras and S. Markovskaja and N. Mesanza and I. Milenković and D.L. Musolin and K. Nikolaou and J.A. Nowakowska and N. Ogris and F. Oskay and T. Oszako and I. Papazova-Anakieva and M. Paraschiv and M. Pasquali and F. Pecori and T. Rafoss and K. Raitelaitytė and R. Raposo and C. Robin and C.A. Rodas and A. Santini A. and A.V. Sanz-Ros and A.V. Selikhovkin and A. Solla and M. Soukainen and N. Soulioti and E.T. Steenkamp and P. Tsopelas and A. Vemić and A.M. Vettraino and M.J. Wingfield and S. Woodward and C. Zamora-Ballesteros C. and M.S. Mullett }, doi = {doi:10.3390/f11070724}, issn = {1999-4907}, year = {2020}, date = {2020-07-01}, journal = {Forests}, volume = {11}, number = {7}, abstract = {Fusarium circinatum, the causal agent of pine pitch canker (PPC), is currently one of the most important threats of Pinus spp. globally. This pathogen is known in many pine-growing regions, including natural and planted forests, and can affect all life stages of trees, from emerging seedlings to mature trees. Despite the importance of PPC, the global distribution of F. circinatum is poorly documented, and this problem is also true of the hosts within countries that are affected. The aim of this study was to review the global distribution of F. circinatum, with a particular focus on Europe. We considered (1) the current and historical pathogen records, both positive and negative, based on confirmed reports from Europe and globally; (2) the genetic diversity and population structure of the pathogen; (3) the current distribution of PPC in Europe, comparing published models of predicted disease distribution; and (4) host susceptibility by reviewing literature and generating a comprehensive list of known hosts for the fungus. These data were collated from 41 countries and used to compile a specially constructed geo-database (http://bit.do/phytoportal). A review of 6297 observation records showed that F. circinatum and the symptoms it causes on conifers occurred in 14 countries, including four in Europe, and is absent in 28 countries. Field observations and experimental data from 138 host species revealed 106 susceptible host species including 85 Pinus species, 6 non-pine tree species and 15 grass and herb species. Our data confirm that susceptibility to F. circinatum varies between different host species, tree ages and environmental characteristics. Knowledge on the geographic distribution, host range and the relative susceptibility of different hosts is essential for disease management, mitigation and containment strategies. The findings reported in this review will support countries that are currently free of F. circinatum in implementing effective procedures and restrictions and prevent further spread of the pathogen.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Fusarium circinatum, the causal agent of pine pitch canker (PPC), is currently one of the most important threats of Pinus spp. globally. This pathogen is known in many pine-growing regions, including natural and planted forests, and can affect all life stages of trees, from emerging seedlings to mature trees. Despite the importance of PPC, the global distribution of F. circinatum is poorly documented, and this problem is also true of the hosts within countries that are affected. The aim of this study was to review the global distribution of F. circinatum, with a particular focus on Europe. We considered (1) the current and historical pathogen records, both positive and negative, based on confirmed reports from Europe and globally; (2) the genetic diversity and population structure of the pathogen; (3) the current distribution of PPC in Europe, comparing published models of predicted disease distribution; and (4) host susceptibility by reviewing literature and generating a comprehensive list of known hosts for the fungus. These data were collated from 41 countries and used to compile a specially constructed geo-database (http://bit.do/phytoportal). A review of 6297 observation records showed that F. circinatum and the symptoms it causes on conifers occurred in 14 countries, including four in Europe, and is absent in 28 countries. Field observations and experimental data from 138 host species revealed 106 susceptible host species including 85 Pinus species, 6 non-pine tree species and 15 grass and herb species. Our data confirm that susceptibility to F. circinatum varies between different host species, tree ages and environmental characteristics. Knowledge on the geographic distribution, host range and the relative susceptibility of different hosts is essential for disease management, mitigation and containment strategies. The findings reported in this review will support countries that are currently free of F. circinatum in implementing effective procedures and restrictions and prevent further spread of the pathogen. |
 | Jančovičová, S; Adamčíková, K; Caboň, M; Adamčík, S How variable is Crepidotus variabilis? Journal Article Phytotaxa, 449 (3), pp. 243-264, 2020, ISSN: 1179-3155. @article{Jančovičová2020, title = {How variable is Crepidotus variabilis?}, author = {S. Jančovičová and K. Adamčíková and M. Caboň and S. Adamčík}, url = {http://ife.sk/wp-content/uploads/2016/10/fig-phytotaxa-sona-300x206.jpg}, doi = {doi.org/10.11646/phytotaxa.449.3.4}, issn = {1179-3155}, year = {2020}, date = {2020-06-23}, journal = {Phytotaxa}, volume = {449}, number = {3}, pages = {243-264}, abstract = {This study evaluates the morphological concept of Crepidotus variabilis and similar taxa based on basidiospore and cheilocystidia characters. A phylogenetic analysis of the ITS region clearly distinguished C. variabilis var. variabilis from the two other distinct taxa of the same lineage, C. variabilis var. trichocystis and C. neotrichocystis. We also recognised one additional morphologically similar and undescribed species that belongs to the C. variabilis lineage. All four taxa can also be recognised morphologically using basidiospore and cheilocystidia characters. Our detailed statistical comparison of these characters improves the knowledge of their variability and specifies more precisely the morphological circumscription of the taxa within this group.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study evaluates the morphological concept of Crepidotus variabilis and similar taxa based on basidiospore and cheilocystidia characters. A phylogenetic analysis of the ITS region clearly distinguished C. variabilis var. variabilis from the two other distinct taxa of the same lineage, C. variabilis var. trichocystis and C. neotrichocystis. We also recognised one additional morphologically similar and undescribed species that belongs to the C. variabilis lineage. All four taxa can also be recognised morphologically using basidiospore and cheilocystidia characters. Our detailed statistical comparison of these characters improves the knowledge of their variability and specifies more precisely the morphological circumscription of the taxa within this group. |
 | Pastirčáková, K; Adamčíková, K; Barta, M; Pažitný, J; Hoťka, P; Sarvašová, I; Kádasi-Horáková, M Host range of Hymenoscyphus fraxineus in Slovak arboreta Journal Article Forests, 11 (5), pp. 1-18, Article Number: 596, 2020, ISSN: 1999-4907. @article{Pastirčáková25.0, title = {Host range of \textit{Hymenoscyphus fraxineus} in Slovak arboreta}, author = {K. Pastirčáková and K. Adamčíková and M. Barta and J. Pažitný and P. Hoťka and I. Sarvašová and M. Kádasi-Horáková}, doi = {10.3390/f11050596}, issn = {1999-4907}, year = {2020}, date = {2020-05-25}, journal = {Forests}, volume = {11}, number = {5}, pages = {1-18, Article Number: 596}, abstract = {The health of 34 different Fraxinus taxa in association with the pathogenic fungus Hymenoscyphus fraxineus was assessed in four Slovak arboreta. Averaged across all arboreta, nearly one-quarter (24.9%) of all evaluated trees showed ash dieback symptoms. The damage was most serious on the common ash F. excelsior, a native species. The percentage of dead trees did not exceed 2% for all evaluated trees. Generally, ash trees of all ages were affected, though the intensity of the damage varied among the sites. The identity of H. fraxineus was confirmed by conventional PCR targeting the internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA, as well as the 18S gene/ITS-2 region of the rDNA operon. In Slovakia, the pathogen has expanded its host range from native species not only to their ornamental cultivars, but also to introduced North American (F. cinerea, F. latifolia, F. pennsylvanica, F. quadrangulata) and Asian (F. bungeana, F. chinensis ssp. rhynchophylla, F. mandshurica) ash species. H. fraxineus was also observed on the previous year’s leaf petioles of the native European species F. ornus, considered a weakly susceptible host. In Slovak arboreta, H. fraxineus was found on 23 Fraxinus taxa; 21 of them represent first records for the country. F. bungeana is recorded as a new host species of H. fraxineus.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The health of 34 different Fraxinus taxa in association with the pathogenic fungus Hymenoscyphus fraxineus was assessed in four Slovak arboreta. Averaged across all arboreta, nearly one-quarter (24.9%) of all evaluated trees showed ash dieback symptoms. The damage was most serious on the common ash F. excelsior, a native species. The percentage of dead trees did not exceed 2% for all evaluated trees. Generally, ash trees of all ages were affected, though the intensity of the damage varied among the sites. The identity of H. fraxineus was confirmed by conventional PCR targeting the internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA, as well as the 18S gene/ITS-2 region of the rDNA operon. In Slovakia, the pathogen has expanded its host range from native species not only to their ornamental cultivars, but also to introduced North American (F. cinerea, F. latifolia, F. pennsylvanica, F. quadrangulata) and Asian (F. bungeana, F. chinensis ssp. rhynchophylla, F. mandshurica) ash species. H. fraxineus was also observed on the previous year’s leaf petioles of the native European species F. ornus, considered a weakly susceptible host. In Slovak arboreta, H. fraxineus was found on 23 Fraxinus taxa; 21 of them represent first records for the country. F. bungeana is recorded as a new host species of H. fraxineus. |
 | Buyck, B; Wang, X H; Adamčíková, K; Caboň, M; Jančovičová, S; Hofstetter, V; Adamčík, S One step closer to unravelling the origin of Russula: subgenus Glutinosae subg. nov. Journal Article Mycosphere, 11 (1), pp. 285–304, 2020, ISSN: 2077 7019. @article{Buyck2020, title = {One step closer to unravelling the origin of Russula: subgenus Glutinosae subg. nov.}, author = {B. Buyck and X. H. Wang and K. Adamčíková and M. Caboň and S Jančovičová and V. Hofstetter and S. Adamčík }, doi = {Doi 10.5943/mycosphere/11/1/6}, issn = {2077 7019}, year = {2020}, date = {2020-01-31}, journal = {Mycosphere}, volume = {11}, number = {1}, pages = {285–304}, abstract = {This study reports on the discovery of a new subgenus, Russula subg. Glutinosae, having an Eastern North American – East Asian distribution. A multigene phylogeny places this new subgenus sister with strong support to a well-supported clade composed of subgenera Compactae and Archaeae. It holds only two very rare, northern hemisphere species, the North American R. glutinosa and the Asian R. glutinosoides sp. nov., thereby adding support to a northern hemisphere origin of the genus. Russula fattoensis is here shown to be a synonym of R. glutinosa. Detailed morphological descriptions and illustrations of holotype collections are provided and potential affinities and similarities with other subgenera are discussed. The new subgenus is a perfect illustration of the fact that nBLAST of nrITS does not always provide the appropriate sampling for phylogenetic analyses.}, keywords = {}, pubstate = {published}, tppubtype = {article} } This study reports on the discovery of a new subgenus, Russula subg. Glutinosae, having an Eastern North American – East Asian distribution. A multigene phylogeny places this new subgenus sister with strong support to a well-supported clade composed of subgenera Compactae and Archaeae. It holds only two very rare, northern hemisphere species, the North American R. glutinosa and the Asian R. glutinosoides sp. nov., thereby adding support to a northern hemisphere origin of the genus. Russula fattoensis is here shown to be a synonym of R. glutinosa. Detailed morphological descriptions and illustrations of holotype collections are provided and potential affinities and similarities with other subgenera are discussed. The new subgenus is a perfect illustration of the fact that nBLAST of nrITS does not always provide the appropriate sampling for phylogenetic analyses. |
 | Adamčík, S; Dima, B; Adamčíková, K; Corriol, G; Læssøe, T; Moreau, P -A; Caboň, M; Jančovičová, S Hodophilus phaeophyllus complex (Clavariaceae, Agaricales) is defined as new phylogenetic lineage in Europe Journal Article Mycological Progress, 19 (2), pp. 111-125, 2020, ISSN: 1617-416X. @article{Adamčík2020, title = {Hodophilus phaeophyllus complex (Clavariaceae, Agaricales) is defined as new phylogenetic lineage in Europe}, author = {S. Adamčík and B. Dima and K. Adamčíková and G. Corriol and T. Læssøe and P.-A. Moreau and M. Caboň and S. Jančovičová }, doi = {DOI 10.1007/s11557-019-01544-9}, issn = {1617-416X}, year = {2020}, date = {2020-01-30}, journal = {Mycological Progress}, volume = {19}, number = {2}, pages = {111-125}, abstract = {The study deals with the last unexplored morphological group of the genus Hodophilus defined by absence of distinct odours, absence of yellow colours and absence of darker dots on the stipe. The phylogenetic reconstruction of the whole genus based on nrITS, nrLSU and RPB2 sequences placed all European members having these morphological characters in a monophyletic group defined here as a new section H. sect. Phaeophylli. The remaining European members of the genus are placed in two additional groups classified as section H. sect. Hodophilus and the new section H. sect. Micacei. Five species are recognised within section Phaeophylli which is typified by H. phaeophyllus that is lecto- and epitypified. Three new species belonging to this section are described: H. carpathicus, H. decurrentior and H. stramineus. Hodophilus decurrentior is the only species showing distinct morphological differences under the microscope. The identification of other species of the section depends mainly on the colour of basidiomata. An updated key to all European members of the genus is provided.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The study deals with the last unexplored morphological group of the genus Hodophilus defined by absence of distinct odours, absence of yellow colours and absence of darker dots on the stipe. The phylogenetic reconstruction of the whole genus based on nrITS, nrLSU and RPB2 sequences placed all European members having these morphological characters in a monophyletic group defined here as a new section H. sect. Phaeophylli. The remaining European members of the genus are placed in two additional groups classified as section H. sect. Hodophilus and the new section H. sect. Micacei. Five species are recognised within section Phaeophylli which is typified by H. phaeophyllus that is lecto- and epitypified. Three new species belonging to this section are described: H. carpathicus, H. decurrentior and H. stramineus. Hodophilus decurrentior is the only species showing distinct morphological differences under the microscope. The identification of other species of the section depends mainly on the colour of basidiomata. An updated key to all European members of the genus is provided. |
 | Elvira-Recuenco, M; Cacciola, S O; Sanz-Ros, A V; Garbelotto, M; Aguayo, J; Solla, A; Mullett, M; Drenkhan, T; Oskay, F; Kaya, A G A; Iturritxa, E; Cleary, M; Witzell, J; Georgieva, M; Papazova-Anakieva, I; Chira, D; Paraschiv, M; Musolin, D L; Selikhovkin, A V; Varentsova, Yu. E; Adamčíková, K; Markovskaja, S; Mesanza, N; Davydenko, K; Capretti, P; Scanu, B; Gonthier, P; Tsopelas, P; Martín-García, J; Morales-Rodríguez, C; Lehtijärvi, A; Lehtijärvi, Doğmuş H T; Oszako, T; Nowakowska, J A; Bragança, H; Fernández-Fernández, M; Hantula, J; Díez, J J Potential Interactions Between Invasive Fusarium circinatum and Other Pine Pathogens in Europe Journal Article Forests, 11 (1), 2020, ISSN: 1999-4907. @article{Elvira-Recuenco2019, title = {Potential Interactions Between Invasive Fusarium circinatum and Other Pine Pathogens in Europe}, author = {M. Elvira-Recuenco and S. O. Cacciola and A. V. Sanz-Ros and M. Garbelotto and J. Aguayo and A. Solla and M. Mullett and T. Drenkhan and F. Oskay and A. G. A. Kaya and E. Iturritxa and M. Cleary and J. Witzell and M. Georgieva and I. Papazova-Anakieva and D. Chira and M. Paraschiv and D.L. Musolin and A.V. Selikhovkin and E. Yu. Varentsova and K. Adamčíková and S. Markovskaja and N. Mesanza and K. Davydenko and P. Capretti and B. Scanu and P. Gonthier and P. Tsopelas and J. Martín-García and C. Morales-Rodríguez and A. Lehtijärvi and H. T. Doğmuş Lehtijärvi and T. Oszako and J.A. Nowakowska and H. Bragança and M. Fernández-Fernández and J. Hantula and J.J. Díez }, doi = {doi:10.3390/f11010007}, issn = {1999-4907}, year = {2020}, date = {2020-01-18}, journal = {Forests}, volume = {11}, number = {1}, abstract = {Pines are major components of native forests and plantations in Europe, where they have both economic significance and an important ecological role. Diseases of pines are mainly caused by fungal and oomycete pathogens, and can significantly reduce the survival, vigor, and yield of both individual trees and entire stands or plantations. Pine pitch canker (PPC), caused by Fusarium circinatum (Nirenberg and O’Donnell), is among the most devastating pine diseases in the world, and is an example of an emergent invasive disease in Europe. The effects of microbial interactions on plant health, as well as the possible roles plant microbiomes may have in disease expression, have been the focus of several recent studies. Here, we describe the possible effects of co-infection with pathogenic fungi and oomycetes with F. circinatum on the health of pine seedlings and mature plants, in an attempt to expand our understanding of the role that biotic interactions may play in the future of PPC disease in European nurseries and forests. The available information on pine pathogens that are able to co-occur with F. circinatum in Europe is here reviewed and interpreted to theoretically predict the effects of such co-occurrences on pine survival, growth, and yield. Beside the awareness that F. circinatum may co-occurr on pines with other pathogens, an additional outcome from this review is an updating of the literature, including the so-called grey literature, to document the geographical distribution of the relevant pathogens and to facilitate differential diagnoses, particularly in nurseries, where some of them may cause symptoms similar to those induced by F. circinatum. An early and accurate diagnosis of F. circinatum, a pathogen that has been recently introduced and that is currently regulated in Europe, is essential to prevent its introduction and spread in plantings and forests.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Pines are major components of native forests and plantations in Europe, where they have both economic significance and an important ecological role. Diseases of pines are mainly caused by fungal and oomycete pathogens, and can significantly reduce the survival, vigor, and yield of both individual trees and entire stands or plantations. Pine pitch canker (PPC), caused by Fusarium circinatum (Nirenberg and O’Donnell), is among the most devastating pine diseases in the world, and is an example of an emergent invasive disease in Europe. The effects of microbial interactions on plant health, as well as the possible roles plant microbiomes may have in disease expression, have been the focus of several recent studies. Here, we describe the possible effects of co-infection with pathogenic fungi and oomycetes with F. circinatum on the health of pine seedlings and mature plants, in an attempt to expand our understanding of the role that biotic interactions may play in the future of PPC disease in European nurseries and forests. The available information on pine pathogens that are able to co-occur with F. circinatum in Europe is here reviewed and interpreted to theoretically predict the effects of such co-occurrences on pine survival, growth, and yield. Beside the awareness that F. circinatum may co-occurr on pines with other pathogens, an additional outcome from this review is an updating of the literature, including the so-called grey literature, to document the geographical distribution of the relevant pathogens and to facilitate differential diagnoses, particularly in nurseries, where some of them may cause symptoms similar to those induced by F. circinatum. An early and accurate diagnosis of F. circinatum, a pathogen that has been recently introduced and that is currently regulated in Europe, is essential to prevent its introduction and spread in plantings and forests. |
2019 |
|
Adamčík, S; Looney, B; Caboň, M; Jančovičová, S; Adamčíková, K; Avis, P G; Barajas, M; Bhatt, R P; Corrales, A; Das, K; Hampe, F; Ghosh, A; Gates, G; Kälviäinen, V; Khalid, A N; Kiran, M; Lange, De R; Lee, H; Lim, Y W; Kong, A; Manz, C; Ovrebo, C; Saba, M; Taipale, T; Verbeken, A; Wisitrassameewong, K; Buyck, B The quest for a globally comprehensible Russula language Journal Article Fungal Diversity, 99 (1), pp. 369-449, 2019, ISSN: 1560-2745. @article{Adamčík2019, title = {The quest for a globally comprehensible Russula language}, author = {S. Adamčík and B. Looney and M. Caboň and S. Jančovičová and K. Adamčíková and P. G. Avis and M. Barajas and R. P. Bhatt and A. Corrales and K. Das and F. Hampe and A. Ghosh and G. Gates and V. Kälviäinen and A. N. Khalid and M. Kiran and R. De Lange and H. Lee and Y. W. Lim and A. Kong and C. Manz and C. Ovrebo and M. Saba and T. Taipale and A. Verbeken and K. Wisitrassameewong and B. Buyck}, doi = {doi.org/10.1007/s13225-019-00437-2}, issn = {1560-2745}, year = {2019}, date = {2019-10-24}, journal = {Fungal Diversity}, volume = {99}, number = {1}, pages = {369-449}, abstract = {Since 2007, the quality of Russula descriptions has improved and the use of molecular support for species delimitation and the number of published new species has increased. However, the description style is not consistent and has regional or author-specific patterns. Most recent publications still favour descriptions of spores compared to hymenium and pileipellis elements, and usually only the spore size is provided with statistical support. This study proposes standards for descriptions of the microscopic structure of Russula species (Russulaceae, Agaricomycetes). We present the description template, the template measurements table, the specific terminology and the essential chemical reagents. The proposed standards were tested by mycologists from 11 countries who met at the Russula Microscopy Workshop in Slovakia. Descriptions of 26 species from 9 countries and four continents were prepared, among them R. amarissima, R. castanopsidis, R. seperina and R. subtilis are re-described and 15 species are introduced as new: R. abietiphila, R. amerorecondita, R. aurantioflava, R. echidna, R. flavobrunnescens, R. fluvialis, R. fortunae, R. garyensis, R. gemmata, R. laevis, R. madrensis, R. olivaceohimalayensis, R. purpureogracilis, R. sancti-pauli and R. wielangtae. Seven descriptions for candidate new species are provided without a formal name assignment. Pairwise comparison of species described in this study with available similar descriptions of related species suggests that microscopic characters from all parts of the basidiomata can be equally important for species recognition and they deserve the same treatment including number of measurements and statistics. The majority of recent studies does not recognise differences between the pileus margin and centre, but more than one-third of the species described in this study show distinct differences between the pileus areas, emphasizing the importance to specify the origin of pileipellis observations. This study proved that there is frequently insufficient difference in the ITS barcode between closely related species and that it is necessary to use more genetic markers combined with ecological and geographical data.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Since 2007, the quality of Russula descriptions has improved and the use of molecular support for species delimitation and the number of published new species has increased. However, the description style is not consistent and has regional or author-specific patterns. Most recent publications still favour descriptions of spores compared to hymenium and pileipellis elements, and usually only the spore size is provided with statistical support. This study proposes standards for descriptions of the microscopic structure of Russula species (Russulaceae, Agaricomycetes). We present the description template, the template measurements table, the specific terminology and the essential chemical reagents. The proposed standards were tested by mycologists from 11 countries who met at the Russula Microscopy Workshop in Slovakia. Descriptions of 26 species from 9 countries and four continents were prepared, among them R. amarissima, R. castanopsidis, R. seperina and R. subtilis are re-described and 15 species are introduced as new: R. abietiphila, R. amerorecondita, R. aurantioflava, R. echidna, R. flavobrunnescens, R. fluvialis, R. fortunae, R. garyensis, R. gemmata, R. laevis, R. madrensis, R. olivaceohimalayensis, R. purpureogracilis, R. sancti-pauli and R. wielangtae. Seven descriptions for candidate new species are provided without a formal name assignment. Pairwise comparison of species described in this study with available similar descriptions of related species suggests that microscopic characters from all parts of the basidiomata can be equally important for species recognition and they deserve the same treatment including number of measurements and statistics. The majority of recent studies does not recognise differences between the pileus margin and centre, but more than one-third of the species described in this study show distinct differences between the pileus areas, emphasizing the importance to specify the origin of pileipellis observations. This study proved that there is frequently insufficient difference in the ITS barcode between closely related species and that it is necessary to use more genetic markers combined with ecological and geographical data. | |
 | K. Adamčíková E. Ondrušková, Kobza M Hypovirulence in chestnut blight fungus, Cryphonectria parasitica, in Slovakia Journal Article Biocontrol Science and Technology, 29 (9), pp. 840-851, 2019, ISSN: 0958-3157. @article{Adamčíková2019, title = {Hypovirulence in chestnut blight fungus, Cryphonectria parasitica, in Slovakia}, author = {K. Adamčíková, E. Ondrušková, M. Kobza}, doi = {DOI: 10.1080/09583157.2019.1608509}, issn = {0958-3157}, year = {2019}, date = {2019-07-19}, journal = {Biocontrol Science and Technology}, volume = {29}, number = {9}, pages = {840-851}, abstract = {The recovery of the European chestnut from chestnut blight in Europe is attributed to hypovirulence, a viral disease that reduces the virulence of its host – Cryphonectria parasitica. Hypovirulence has been used in biocontrol programmes throughout Europe with the aim of not only treating individual chestnut blight cankers but causing the natural spread and establishment of viruses in C. parasitica populations. Almost 40 years after the first appearance of chestnut blight and more than 20 years after the first biocontrol treatment implementation in Slovakia the establishment and natural spread of hypovirulence were studied. The morphological characteristics of 179 chestnut blight cankers and 161 C. parasitica strains were evaluated for the presence of hypovirus from three chestnut cultivation sites in Slovakia. Nineteen cankers and isolates were considered hypovirulent based on visual assessment of their morphological characteristics. The hypovirus presence by RNA extraction was confirmed in 6 cankers/strains.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The recovery of the European chestnut from chestnut blight in Europe is attributed to hypovirulence, a viral disease that reduces the virulence of its host – Cryphonectria parasitica. Hypovirulence has been used in biocontrol programmes throughout Europe with the aim of not only treating individual chestnut blight cankers but causing the natural spread and establishment of viruses in C. parasitica populations. Almost 40 years after the first appearance of chestnut blight and more than 20 years after the first biocontrol treatment implementation in Slovakia the establishment and natural spread of hypovirulence were studied. The morphological characteristics of 179 chestnut blight cankers and 161 C. parasitica strains were evaluated for the presence of hypovirus from three chestnut cultivation sites in Slovakia. Nineteen cankers and isolates were considered hypovirulent based on visual assessment of their morphological characteristics. The hypovirus presence by RNA extraction was confirmed in 6 cankers/strains. |
 | Pažitný, J; Kobza, M; Adamčíková, K Variance in leaf spot susceptibility in chestnut trees of different species and hybrids Journal Article Journal of Plant Disease and Protection, 126 (2), pp. 169-172, 2019, ISSN: 1861-3829. @article{Pažitný2019, title = {Variance in leaf spot susceptibility in chestnut trees of different species and hybrids}, author = {J. Pažitný and M. Kobza and K. Adamčíková}, url = {https://link.springer.com/article/10.1007/s41348-019-00209-9}, doi = {DOI 10.1007/s41348-019-00209-9}, issn = {1861-3829}, year = {2019}, date = {2019-02-19}, journal = {Journal of Plant Disease and Protection}, volume = {126}, number = {2}, pages = {169-172}, abstract = {Prior to the first occurrence of chestnut blight in 1976, leaf spot caused by Cylindrosporium castaneae was one of the most important diseases of European chestnut trees in Slovakia. In 2011, extensive damage of leaves by leaf spot was recorded in young chestnut trees in an experimental chestnut orchard in Pribelce, Slovakia, where seedlings and grafts of different taxonomic origin (Castanea sativa, Castanea crenata, and C. sativa × C. crenata) exhibited visible differences in the extent of leaf spot. Digital images of particular leaves were evaluated using Quick Micro Photo software and the ratio of infected leaf area was calculated. Morphological observations of the fungal species causing leaf spot were conducted and the disease symptoms were described and discussed. Taxonomic origin of studied trees showed a significant effect on the proportion of leaf spot area, indicating that the response to fungal attacks has a strong genetic determinism, with resistance being clearly influenced by taxonomic origin. The lowest leaf spot infection was recorded in C. crenata seedlings. Significant differences were noted between the individual trees tested and between the studied years.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Prior to the first occurrence of chestnut blight in 1976, leaf spot caused by Cylindrosporium castaneae was one of the most important diseases of European chestnut trees in Slovakia. In 2011, extensive damage of leaves by leaf spot was recorded in young chestnut trees in an experimental chestnut orchard in Pribelce, Slovakia, where seedlings and grafts of different taxonomic origin (Castanea sativa, Castanea crenata, and C. sativa × C. crenata) exhibited visible differences in the extent of leaf spot. Digital images of particular leaves were evaluated using Quick Micro Photo software and the ratio of infected leaf area was calculated. Morphological observations of the fungal species causing leaf spot were conducted and the disease symptoms were described and discussed. Taxonomic origin of studied trees showed a significant effect on the proportion of leaf spot area, indicating that the response to fungal attacks has a strong genetic determinism, with resistance being clearly influenced by taxonomic origin. The lowest leaf spot infection was recorded in C. crenata seedlings. Significant differences were noted between the individual trees tested and between the studied years. |
2018 |
|
 | Bolvanský, M; Pažitný, J; Adamčíková, K Grading of blight resistance in different chestnut accessions. Journal Article Acta Horticulturae, 1220 , pp. 87-94, 2018, ISSN: 05677572. @article{M.2018, title = {Grading of blight resistance in different chestnut accessions.}, author = {M. Bolvanský and J. Pažitný and K. Adamčíková}, url = {https://www.actahort.org/members/showpdf?booknrarnr=1220_13}, doi = {10.17660/ActaHortic.2018.1220.13}, issn = {05677572}, year = {2018}, date = {2018-11-01}, journal = {Acta Horticulturae}, volume = {1220}, pages = {87-94}, abstract = {Five chestnut trees (three hybrids Castanea sativa × C. crenata, one C. sativa and one C. crenata) with different levels of blight resistance were subjected to the screening by two virulent and one hypovirulent strains of Cryphonectria parasitica in order to examine reliability of ex situ tests for grading of blight resistance in chestnut. Branches 2-3 cm in diameter were harvested from the trees, in early April and end of September and stored at about 10°C for either 2 weeks (for branch sections) or 4 weeks (for bark- and wood-tissue sections). Branch sections and bark- and wood-tissue sections were inoculated with the C. parasitica strains and incubated in the dark at 25°C with 95% humidity for 4 weeks and/or 4 days, respectively. In branch sections, superficial cankers were very rare so only occurrence of pycnidia formed around the inoculating holes were evaluated. Pycnidia occurred more frequently in branch sections collected from the less resistant accessions in October trial. The size of necrotic areas reflected the resistance ranking of accessions in April/May better than in October inoculation trials. Results of inoculations on excised wood tissue were more consistent with the real blight resistance level than data obtained from inoculations on excised bark tissue. Occasional inconsistence between results of inoculations carried out at different dates or in different trees might be caused by different physiological state of collected branches. Based on these results it can be concluded that the end of dormant period seems to be the most suitable time to carry out both pathogenicity and resistance tests in chestnuts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Five chestnut trees (three hybrids Castanea sativa × C. crenata, one C. sativa and one C. crenata) with different levels of blight resistance were subjected to the screening by two virulent and one hypovirulent strains of Cryphonectria parasitica in order to examine reliability of ex situ tests for grading of blight resistance in chestnut. Branches 2-3 cm in diameter were harvested from the trees, in early April and end of September and stored at about 10°C for either 2 weeks (for branch sections) or 4 weeks (for bark- and wood-tissue sections). Branch sections and bark- and wood-tissue sections were inoculated with the C. parasitica strains and incubated in the dark at 25°C with 95% humidity for 4 weeks and/or 4 days, respectively. In branch sections, superficial cankers were very rare so only occurrence of pycnidia formed around the inoculating holes were evaluated. Pycnidia occurred more frequently in branch sections collected from the less resistant accessions in October trial. The size of necrotic areas reflected the resistance ranking of accessions in April/May better than in October inoculation trials. Results of inoculations on excised wood tissue were more consistent with the real blight resistance level than data obtained from inoculations on excised bark tissue. Occasional inconsistence between results of inoculations carried out at different dates or in different trees might be caused by different physiological state of collected branches. Based on these results it can be concluded that the end of dormant period seems to be the most suitable time to carry out both pathogenicity and resistance tests in chestnuts. |
 | Adamčíková, K; Pažitný, J; Pastirčáková, K Individual resistance of Fraxinus angustifolia and F. excelsior clones to Hymenoscyphus fraxineus Journal Article Journal of Plant Protection Research, 58 (3), pp. 227-233, 2018, ISSN: 1427-4345. @article{Adamčíková2018, title = {Individual resistance of \textit{Fraxinus angustifolia} and \textit{F. excelsior} clones to \textit{Hymenoscyphus fraxineus}}, author = {K. Adamčíková and J. Pažitný and K. Pastirčáková}, doi = {10.24425/122937}, issn = {1427-4345}, year = {2018}, date = {2018-10-22}, journal = {Journal of Plant Protection Research}, volume = {58}, number = {3}, pages = {227-233}, abstract = {Ash dieback, caused by Hymenoscyphus fraxineus, is a serious disease of common and narrow-leaved ash in Europe. The resistance of individual trees seems to be important for the maintenance of ash in European forests. In this in situ wound inoculation study, the susceptibility and differences in resistance to H. fraxineus between Fraxinus excelsior and F. angustifolia clones were assessed. Neither of the tested clones revealed total resistance to ash dieback; variety between the tested clones was observed. Differences in necroses lengths were significant between clones and between two ash species. Longer necroses were formed in F. angustifolia than in F. excelsior. Some clones exhibiting some resistance to the pathogen were identified. }, keywords = {}, pubstate = {published}, tppubtype = {article} } Ash dieback, caused by Hymenoscyphus fraxineus, is a serious disease of common and narrow-leaved ash in Europe. The resistance of individual trees seems to be important for the maintenance of ash in European forests. In this in situ wound inoculation study, the susceptibility and differences in resistance to H. fraxineus between Fraxinus excelsior and F. angustifolia clones were assessed. Neither of the tested clones revealed total resistance to ash dieback; variety between the tested clones was observed. Differences in necroses lengths were significant between clones and between two ash species. Longer necroses were formed in F. angustifolia than in F. excelsior. Some clones exhibiting some resistance to the pathogen were identified. |
 | Pastirčáková, K; Adamčíková, K; Pastirčák, M; Zach, P; Galko, J; Kováč, M; Laco, J Two blue-stain fungi colonizing Scots pine (Pinus sylvestris) trees infested by bark beetles in Slovakia, Central Europe Journal Article Biologia, 73 (11), pp. 1053-1066, 2018, ISSN: 0006-3088. @article{Pastirčáková2018c, title = {Two blue-stain fungi colonizing Scots pine (\textit{Pinus sylvestris}) trees infested by bark beetles in Slovakia, Central Europe}, author = {K. Pastirčáková and K. Adamčíková and M. Pastirčák and P. Zach and J. Galko and M. Kováč and J. Laco}, doi = {10.2478/s11756-018-0114-6}, issn = {0006-3088}, year = {2018}, date = {2018-09-17}, journal = {Biologia}, volume = {73}, number = {11}, pages = {1053-1066}, abstract = {Excessive mortality of Scots pine (Pinus sylvestris) trees has recently been observed in the Záhorská nížina lowland in western Slovakia, Central Europe. Continuous drought, bark beetles and blue-stain fungi are likely to play a crucial role in this dieback of pines in a warm sandy area. Two blue-stain fungi, Ophiostoma ips and Ophiostoma minus, were found here on Scots pine trunks infested by bark beetles. These fungi were isolated and identified based on morphological properties and DNA sequences (ITS, LSU, β-tubulin). They colonized the inner bark, phloem and blue-stained sapwood, and were recorded in the galleries of Orthotomicus longicollis and other species of this genus. The occurrence of O. minus was massive; the fungus was found to produce perithecia abundantly in dying pines. Detailed descriptions, illustrations and global distribution of the two fungal species on host pine species are provided. The records of O. ips and those of O. minus are new for the mycoflora of Slovakia. Undetermined mycophagous nematodes were found inside the perithecia of O. ips. The role of bark beetles as real and potential vectors (Ips spp., Orthotomicus spp., Tomicus spp., Hylurgus ligniperda, Hylastes sp.) of blue-stain fungi in a declining pine forest is discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Excessive mortality of Scots pine (Pinus sylvestris) trees has recently been observed in the Záhorská nížina lowland in western Slovakia, Central Europe. Continuous drought, bark beetles and blue-stain fungi are likely to play a crucial role in this dieback of pines in a warm sandy area. Two blue-stain fungi, Ophiostoma ips and Ophiostoma minus, were found here on Scots pine trunks infested by bark beetles. These fungi were isolated and identified based on morphological properties and DNA sequences (ITS, LSU, β-tubulin). They colonized the inner bark, phloem and blue-stained sapwood, and were recorded in the galleries of Orthotomicus longicollis and other species of this genus. The occurrence of O. minus was massive; the fungus was found to produce perithecia abundantly in dying pines. Detailed descriptions, illustrations and global distribution of the two fungal species on host pine species are provided. The records of O. ips and those of O. minus are new for the mycoflora of Slovakia. Undetermined mycophagous nematodes were found inside the perithecia of O. ips. The role of bark beetles as real and potential vectors (Ips spp., Orthotomicus spp., Tomicus spp., Hylurgus ligniperda, Hylastes sp.) of blue-stain fungi in a declining pine forest is discussed. |